Optimisation of the US EPA (1991) 200.1 pH 1.75 ± 0.1 extraction method for measuring potentially bioavailable chromium (III) - 2025

2025-11-21

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This document describes the validation study performed to optimise the US EPA (1991) method for the measurement of potentially bioavailable chromium using extractions at pH 1.75 ± 0.1 from freshwater and seawater samples. The recommended method provides reasonable recovery of chromium from all types of water tested. The method is applicable for measuring dissolved and precipitated/lightly adsorbed fractions of chromium in fresh and marine water. It should be noted that in an aquatic environment, some of this chromium may ultimately not be readily accessible for aquatic organisms.

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ANZG report - the optimisation method for measuring chromium (III) - 2025 (PDF 2.3 MB)
ANZG report - the optimisation method for measuring chromium (III) – 2025 (DOCX 2.1 MB)

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