2,4-Dichloroaniline in freshwater and marine water

​​Toxicant default guideline values for protecting aquatic ecosystems

October 2000

Extracted from Section 8.3.7 ‘Detailed descriptions of chemicals’ of the ANZECC & ARMCANZ (2000) guidelines.

The default guideline values (previously known as ‘trigger values’) and associated information in this technical brief should be used in accordance with the detailed guidance provided in the Australian and New Zealand Guidelines for Fresh and Marine Water Quality.

Description of chemical

2,4-Dichloroaniline (CAS 55-40-07)

Aquatic toxicology

Freshwater fish: five species, 96-h LC50, 6900-48,000 µg/L. Chronic NOEC data were available for Gasterosteus aculeatus (35 d, growth and mortality) of 330 and 580 µg/L respectively, giving an acute-to-chronic ratio (ACR) up to 28.

Freshwater crustaceans: one species, Daphnia magna, 48-h LC50 or EC50 immobilisation 500-2700 µg/L. Chronic NOEC (16 d, growth) of 15 µg/L.

Freshwater algae, diatoms and blue–green algae: five species, 96-h EC50 (growth) of 400-14,000 µg/L.

Marine diatom: one species, 72-h EC50 (population growth) of 1100 µg/L.


A freshwater moderate reliability trigger value of 7 µg/L was derived for 2,4-DCA using the statistical distribution method (95% protection) and an ACR of 32. In the absence of marine data, this figure was adopted as a marine low reliability trigger value to be used only as an indicative interim working level. This figure is around or slightly below the current analytical practical quantitation limit (PQL).


ANZECC & ARMCANZ 2000. Australian and New Zealand Guidelines for Fresh and Marine Water Quality, Australian and New Zealand Environment and Conservation Council and Agriculture and Resource Management Council of Australia and New Zealand, Canberra.