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[null,null,null,null,"Ecotoxicity Data Quality Assessment Method V2"]
[null,null,null,null,null,"To be used to assess the quality of data used for the Australian and New Zealand Guidelines for Fresh and Marine Water Quality (including sediment) and the Australian National Environment Protection (Assessment of Site Contamination) Measure"]
[]
[]
[]
[null,null,null,null,null,null,"Developed by "]
[]
[]
[null,null,null,null,null,null,"Zoe Zhang"]
[null,null,null,null,null,null,"   Dr. Michael Warne"]
[null,null,null,null,null,null,"Alison Vieritz "]
[]
[]
[]
[null,null,null,null,null,null,"Water Quality & Investigations "]
[null,null,null,null,null,"                 Department of Science,Information Technology and Innovation"]

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["Chemical:","Iron"]
["Medium","Freshwater"]
["Category","Paper and data unique identifier(eg. 1-1,10-150)","Total score","Quality Score","Comment ","Species (Scientific name)","Lifestage","Exposure duration","Measure of toxicity","Toxicity endpoint","Toxicity value","Taxonomic group","Phylum","Class","Media","Temperature ","Acute Or Chronic ","PH","Salinity/Conductivity","Citation","Citation No."]
["Freshwater/metal/plant","150-1",67,0.69072164948453596,"The highest possible grade for this datum is acceptable.","Lemna disperma","frond","7 days","EC50","growth","100521 (nominal)","Macrophyte","Charophyta","Equisetopsida","OECD SIS Lemna growth media","24°C","Chronic",7,"N.A.","Abdul JM, Colville A, Lim R, Vigneswaran S and Kandasamy J (2012). Use of duckweed (Lemna disperma) to assess the phytotoxicity of the products of Fenton oxidation of metsulfuron methyl. Ecotoxicology and Environmental Safety, 83, 89-95. ","150","Note: converted toxicity value from 1.8 mM Fe2SO4.7H2O"]
["Freshwater/metal/non- plant","151-1",48,0.48,"The highest possible grade for this datum is acceptable.","Cyprinus carpio","juvenile","96 h","LC50","Mortality","560 (nominal)","Fish","Chordata","Actinopterygii","Freshwater","15°C","Acute ","Not Reported","N.A.","Alam MK and Maughan OE (1992). The effect of malathion, diazinon, and various concentrations of zinc, copper, nickel, lead, iron, and mercury on fish. Biological Trace Element Research, 34(3), 225-236.","151"]
["Freshwater/metal/non- plant","151-2",48,0.48,"The highest possible grade for this datum is acceptable.","Cyprinus carpio","juvenile","96 h","LC50","Mortality","1360 (nominal)","Fish","Chordata","Actinopterygii","Freshwater","15°C","Acute ","Not Reported","N.A.","Alam MK and Maughan OE (1992). The effect of malathion, diazinon, and various concentrations of zinc, copper, nickel, lead, iron, and mercury on fish. Biological Trace Element Research, 34(3), 225-236.","151"]
["Freshwater/metal/non- plant","151-3",48,0.48,"The highest possible grade for this datum is acceptable.","Cyprinus carpio","juvenile","96 h","LC50","Mortality","1220 (nom)","Fish","Chordata","Actinopterygii","Freshwater","15°C","Acute ","Not Reported","N.A.","Alam MK and Maughan OE (1992). The effect of malathion, diazinon, and various concentrations of zinc, copper, nickel, lead, iron, and mercury on fish. Biological Trace Element Research, 34(3), 225-236.","151"]
["Freshwater/metal/non- plant","151-4",48,0.48,"The highest possible grade for this datum is acceptable.","Cyprinus carpio","juvenile","96 h","LC50","Mortality","2250 (nom)","Fish","Chordata","Actinopterygii","Freshwater","15°C","Acute ","Not Reported","N.A.","Alam MK and Maughan OE (1992). The effect of malathion, diazinon, and various concentrations of zinc, copper, nickel, lead, iron, and mercury on fish. Biological Trace Element Research, 34(3), 225-236.","151"]
["Freshwater/metal/non- plant","152-1",54,0.54,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonate","32 h","Threshold conc","Immobility","44759 (nom)","Microinvertebrate","Arthropoda","Branchiopoda","Lake Erie water","25°C","Acute ","Not Reported","N.A.","Anderson BG (1944). The toxicity thresholds of various substances found in industrial wastes as determined by the use of Daphnia magna. Sewage Works Jour, 16((6)), 1156-1165. ","152","No QA acceptability criteria",null,null,"pH of all solutions above 63.4 mg/L were lower than 7. No effect seen at concentrations higher than this so pH was not an effect."]
["Freshwater/metal/non- plant","152-2",54,0.54,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonate","32 h","Threshold conc","Immobility","< 55880 (nom)","Microinvertebrate","Arthropoda","Branchiopoda","Lake Erie water","25°C","Acute ","Not Reported","N.A.","Anderson BG (1944). The toxicity thresholds of various substances found in industrial wastes as determined by the use of Daphnia magna. Sewage Works Jour, 16((6)), 1156-1165. ","152","No QA acceptability criteria",null,null,"pH of all solutions above 63.4 mg/L were lower than 7. No effect seen at concentrations higher than this so pH was not an effect."]
["Freshwater/metal/plant","153-1",63,0.64948453608247425,"The highest possible grade for this datum is acceptable.","Phragmites australis","seedling","64 d","NOEC","Growth (root length)","1000 (nom)","Macrophyte","Tracheophyta","Liliopsida","10% Rorison's solution","14 (night) 20 (day)°C","Chronic",6,"N.A.","Batty LC and Younger PL (2002). Critical role of macrophytes in achieving low iron concentrations in mine water treatment wetlands. Environmental Science & Technology, 36(18), 3997-4002. ","153","No QA acceptability criteria"]
["Freshwater/metal/plant","153-2",63,0.64948453608247425,"The highest possible grade for this datum is acceptable.","Phragmites australis","seedling","64 d","NOEC","Growth (root:shoot ratio)","1000 (nominal)","Macrophyte","Tracheophyta","Liliopsida","10% Rorison's solution","14 (night) 20 (day)°C","Chronic",6,"N.A.","Batty LC and Younger PL (2002). Critical role of macrophytes in achieving low iron concentrations in mine water treatment wetlands. Environmental Science & Technology, 36(18), 3997-4002. ","153","No QA acceptability criteria"]
["Freshwater/metal/plant","153-3",63,0.64948453608247425,"The highest possible grade for this datum is acceptable.","Phragmites australis","seedling","64 d","NOEC","Growth (dry weight, shoots)","2000 (nom)","Macrophyte","Tracheophyta","Liliopsida","10% Rorison's solution","14 (night) 20 (day)°C","Chronic",6,"N.A.","Batty LC and Younger PL (2002). Critical role of macrophytes in achieving low iron concentrations in mine water treatment wetlands. Environmental Science & Technology, 36(18), 3997-4002. ","153","No QA acceptability criteria"]
["Freshwater/metal/plant","153-4",63,0.64948453608247425,"The highest possible grade for this datum is acceptable.","Phragmites australis","seedling","64 d","NOEC","Growth (dry weight, roots)","2000 (nom)","Macrophyte","Tracheophyta","Liliopsida","10% Rorison's solution","14 (night) 20 (day)°C","Chronic",6,"N.A.","Batty LC and Younger PL (2002). Critical role of macrophytes in achieving low iron concentrations in mine water treatment wetlands. Environmental Science & Technology, 36(18), 3997-4002. ","153","No QA acceptability criteria"]
["Freshwater/metal/plant","153-5",63,0.64948453608247425,"The highest possible grade for this datum is acceptable.","Phragmites australis","seedling","64 d","NOEC","Growth (dry weight, rhizomes)","2000 (nom)","Macrophyte","Tracheophyta","Liliopsida","10% Rorison's solution","14 (night) 20 (day)°C","Chronic",6,"N.A.","Batty LC and Younger PL (2002). Critical role of macrophytes in achieving low iron concentrations in mine water treatment wetlands. Environmental Science & Technology, 36(18), 3997-4002. ","153","No QA acceptability criteria"]
["Freshwater/metal/plant","154-1",56,0.57731958762886593,"The highest possible grade for this datum is acceptable.","Alatospora acuminata","Not reported","21 d","NOEC","Growth (dry weight)",">6900 (nom)","Fungi","Ascomycota","Leotiomycetes","Basal medium","15°C","Chronic",7,"N.A.","Bermingham S, Maltby L and Cooke RC (1996). Effects of a coal mine effluent on aquatic hyphomycetes .2. Laboratory toxicity experiments. Journal of Applied Ecology, 33(6), 1322-1328. ","154","No QA acceptability criteria"]
["Freshwater/metal/plant","154-2",56,0.57731958762886593,"The highest possible grade for this datum is acceptable.","Tetrachaetum elegans","Not reported","21 d","NOEC","Growth (dry weight)",">6900 (nom)","Fungi","Ascomycota","Ascomycetes","Basal medium","15°C","Chronic",7,"N.A.","Bermingham S, Maltby L and Cooke RC (1996). Effects of a coal mine effluent on aquatic hyphomycetes .2. Laboratory toxicity experiments. Journal of Applied Ecology, 33(6), 1322-1328. ","154","No QA acceptability criteria"]
["Freshwater/metal/plant","154-3",56,0.57731958762886593,"The highest possible grade for this datum is acceptable.","Articulospora tetracladia","Not reported","21 d","NOEC","Growth (dry weight)",">6900 (nom)","Fungi","Ascomycota","Leotiomycetes","Basal medium","15°C","Chronic",7,"N.A.","Bermingham S, Maltby L and Cooke RC (1996). Effects of a coal mine effluent on aquatic hyphomycetes .2. Laboratory toxicity experiments. Journal of Applied Ecology, 33(6), 1322-1328. ","154","No QA acceptability criteria"]
["Freshwater/metal/non- plant","155-1",60,0.6,"The highest possible grade for this datum is acceptable.","Tetrahymena pyriformis","exponentially growing","1 h","IC50","esterase activity","2500 (nominal)","Protozoan","Ciliophora","Oligohymenophorea","Proteose-peptone yeast salts ","28°C","Acute ","5.5-9","N.A.","Bogaerts P, Bohatier J and Bonnemoy F (2001). Use of the ciliated protozoan Tetrahymena pyriformis for the assessment of toxicity and quantitative structure-activity relationships of xenobiotics: Comparison with the Microtox test. Ecotoxicology and Environmental Safety, 49(3), 293-301. ","155","No QA acceptability criteria"]
["Freshwater/metal/non- plant","155-2",62,0.62,"The highest possible grade for this datum is acceptable.","Tetrahymena pyriformis","exponentially growing","9 h","IC50","Growth (relative generation time)",">50000 (nominal)","Protozoan","Ciliophora","Oligohymenophorea","Proteose-peptone yeast salts ","28°C","Chronic","Not Reported","N.A.","Bogaerts P, Bohatier J and Bonnemoy F (2001). Use of the ciliated protozoan Tetrahymena pyriformis for the assessment of toxicity and quantitative structure-activity relationships of xenobiotics: Comparison with the Microtox test. Ecotoxicology and Environmental Safety, 49(3), 293-301. ","155","No QA acceptability criteria",null,null,null,"Note: this data is from 156-1, where more detail given, inlcuding Control growth results"]
["Freshwater/metal/non- plant","155-3",62,0.62,"The highest possible grade for this datum is acceptable.","Tetrahymena pyriformis","exponentially growing","9 h","IC50","Growth (relative generation time)",">52200 (nominal)","Protozoan","Ciliophora","Oligohymenophorea","Proteose-peptone yeast salts ","28°C","Chronic","Not Reported","N.A.","Bogaerts P, Bohatier J and Bonnemoy F (2001). Use of the ciliated protozoan Tetrahymena pyriformis for the assessment of toxicity and quantitative structure-activity relationships of xenobiotics: Comparison with the Microtox test. Ecotoxicology and Environmental Safety, 49(3), 293-301. ","155","No QA acceptability criteria"]
["Freshwater/metal/non- plant","155-4",57,0.56999999999999995,"The highest possible grade for this datum is acceptable.","Tetrahymena pyriformis","exponentially growing","1 h","IC50","esterase activity",">100000 (nominal)","Protozoan","Ciliophora","Oligohymenophorea","Proteose-peptone yeast salts ","28°C","Acute ","5.5-9","N.A.","Bogaerts P, Bohatier J and Bonnemoy F (2001). Use of the ciliated protozoan Tetrahymena pyriformis for the assessment of toxicity and quantitative structure-activity relationships of xenobiotics: Comparison with the Microtox test. Ecotoxicology and Environmental Safety, 49(3), 293-301. ","155","No QA acceptability criteria"]
["Freshwater/metal/non- plant","155-5",46,0.46,"The highest possible grade for this datum is acceptable.","Vibrio fisheri","N.A.","30 min","EC50","luminescence inhibition","81000 (nominal)","Protozoan","Ciliophora","Oligohymenophorea","Proteose-peptone yeast salts ","28°C","Acute ","Not Reported","N.A.","Bogaerts P, Bohatier J and Bonnemoy F (2001). Use of the ciliated protozoan Tetrahymena pyriformis for the assessment of toxicity and quantitative structure-activity relationships of xenobiotics: Comparison with the Microtox test. Ecotoxicology and Environmental Safety, 49(3), 293-301. ","155","No QA acceptability criteria"]
["Freshwater/metal/non- plant","155-6",46,0.46,"The highest possible grade for this datum is acceptable.","Vibrio fisheri","N.A.","30 min","EC50","luminescence inhibition","28000 (nominal)","Protozoan","Ciliophora","Oligohymenophorea","Proteose-peptone yeast salts ","28°C","Acute ","Not Reported","N.A.","Bogaerts P, Bohatier J and Bonnemoy F (2001). Use of the ciliated protozoan Tetrahymena pyriformis for the assessment of toxicity and quantitative structure-activity relationships of xenobiotics: Comparison with the Microtox test. Ecotoxicology and Environmental Safety, 49(3), 293-301. ","155","No QA acceptability criteria"]
["Freshwater/metal/non- plant","156-1",69,0.69,"The highest possible grade for this datum is acceptable.","Tetrahymena pyriformis","exponentially growing","1 h","EC50","esterase activity","2500 (nominal)","Protozoan","Ciliophora","Oligohymenophorea","Proteose-peptone yeast salts ","25°C","Acute ",7,"N.A.","Bogaerts P, Senaud J and Bohatier J (1998). Bioassay technique using nonspecific esterase activities of Tetrahymena pyriformis for screening and assessing cytotoxicity of xenobiotics. Environmental Toxicology and Chemistry, 17(8), 1600-1605. ","156"]
["Freshwater/metal/non- plant","157-1",89,0.89,"","Hyalella azteca","1-11 d old","7 d","LC50","Mortality",">15 (diss), >1000 (total)","macroinvertebrate","Arthropoda","Maxillopoda","Freshwater","25°C","Acute ",7.4,"N.A.","Borgmann U, Couillard Y, Doyle P and Dixon DG (2005). Toxicity of sixty‐three metals and metalloids to Hyalella azteca at two levels of water hardness. Environmental Toxicology and Chemistry, 24(3), 641-652. ","157"]
["Freshwater/metal/non- plant","157-2",87,0.87,"The highest possible grade for this datum is acceptable.","Hyalella azteca","1-11 d old","7 d","LC50","Mortality",">3150 (total)","macroinvertebrate","Arthropoda","Maxillopoda","Freshwater","25°C","Acute ",8.4,"N.A.","Borgmann U, Couillard Y, Doyle P and Dixon DG (2005). Toxicity of sixty‐three metals and metalloids to Hyalella azteca at two levels of water hardness. Environmental Toxicology and Chemistry, 24(3), 641-652. ","157"]
["Freshwater/metal/non- plant","158-1",57,0.56999999999999995,"","Austro-potamobius pallipes pallipes","19-32 mm body length, stage C4 of intermoult","96 h","LC50","Immobility","18500 (total?)","macroinvertebrate","Arthropoda","Malocostraca","Pond water","16°C","Acute ",7,"N.A.","Boutet C and Chaisemartin C (1973). Specific Toxic Properties of Metallic Salts in Austro-potamobius pallipes pallipes and Orconectes limosus (Proprietes Toxiques Specifiques des sels Metalliques chez Austropotamobius pallipes pallipes et Orconectes limosu). Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales, 167(12), 1933-1938. ","158"]
["Freshwater/metal/non- plant","158-2",57,0.56999999999999995,"","Austropotamobius pallipes","19-32 mm body length, stage C4 of intermoult","30 d","EC50","Immobility","13900 (total?)","macroinvertebrate","Arthropoda","Malocostraca","Pond water","16°C","Chronic",7,"N.A.","Boutet C and Chaisemartin C (1973). Specific Toxic Properties of Metallic Salts in Austro-potamobius pallipes pallipes and Orconectes limosus (Proprietes Toxiques Specifiques des sels Metalliques chez Austropotamobius pallipes pallipes et Orconectes limosu). Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales, 167(12), 1933-1938. ","158"]
["Freshwater/metal/non- plant","158-3",57,0.56999999999999995,"","Austropotamobius pallipes","19-32 mm body length, stage C4 of intermoult","30 d","EC50","Immobility","13200 (total?)","macroinvertebrate","Arthropoda","Malocostraca","Pond water","16°C","Chronic",7,"N.A.","Boutet C and Chaisemartin C (1973). Specific Toxic Properties of Metallic Salts in Austro-potamobius pallipes pallipes and Orconectes limosus (Proprietes Toxiques Specifiques des sels Metalliques chez Austropotamobius pallipes pallipes et Orconectes limosu). Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales, 167(12), 1933-1938. ","158"]
["Freshwater/metal/non- plant","158-4",57,0.56999999999999995,"","Orconectes limosus","19-32 mm body length, stage C4 of intermoult","30 d","EC50","Immobility","22000 (total?)","macroinvertebrate","Arthropoda","Malocostraca","Pond water","16°C","Chronic",7,"N.A.","Boutet C and Chaisemartin C (1973). Specific Toxic Properties of Metallic Salts in Austro-potamobius pallipes pallipes and Orconectes limosus (Proprietes Toxiques Specifiques des sels Metalliques chez Austropotamobius pallipes pallipes et Orconectes limosu). Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales, 167(12), 1933-1938. ","158"]
["Freshwater/metal/non- plant","158-5",57,0.56999999999999995,"","Orconectes limosus","19-32 mm body length, stage C4 of intermoult","30 d","EC50","Immobility","21000 (total?)","macroinvertebrate","Arthropoda","Malocostraca","Pond water","16°C","Chronic",7,"N.A.","Boutet C and Chaisemartin C (1973). Specific Toxic Properties of Metallic Salts in Austro-potamobius pallipes pallipes and Orconectes limosus (Proprietes Toxiques Specifiques des sels Metalliques chez Austropotamobius pallipes pallipes et Orconectes limosu). Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales, 167(12), 1933-1938. ","158"]
["Freshwater/metal/non- plant","158-6",57,0.56999999999999995,"","Orconectes limosus","19-32 mm body length, stage C4 of intermoult","96 h","EC50","Immobility","32000 (total?)","macroinvertebrate","Arthropoda","Malocostraca","Pond water","16°C","Acute",7,"N.A.","Boutet C and Chaisemartin C (1973). Specific Toxic Properties of Metallic Salts in Austro-potamobius pallipes pallipes and Orconectes limosus (Proprietes Toxiques Specifiques des sels Metalliques chez Austropotamobius pallipes pallipes et Orconectes limosu). Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales, 167(12), 1933-1938. ","158"]
["Freshwater/metal/non- plant","159-1",64,0.64,"","Philodina acuticornis","mixed age","24 h","EC50","Immobility","19100 (total?)","Rotifer","Rotifera","Eurotatoria","Freshwater","20°C","Acute ",7.5,"N.A.","Buikema Jr A, See C and Cairns Jr J (1977). Rotifer sensitivity to combinations of inorganic water pollutants. Bulletin (Virginia Polytechnic Institute and State University. Water Resources Research Center), 92, 42. ","159","Note - refers to APHA, but questionable if followed exact method, so not sure about QA criteria"]
["Freshwater/metal/non- plant","159-2",64,0.64,"","Philodina acuticornis","mixed age","48 h","EC50","Immobility","11300 (total?)","Rotifer","Rotifera","Eurotatoria","Freshwater","20°C","Acute ",7.5,"N.A.","Buikema Jr A, See C and Cairns Jr J (1977). Rotifer sensitivity to combinations of inorganic water pollutants. Bulletin (Virginia Polytechnic Institute and State University. Water Resources Research Center), 92, 42. ","159","Note - refers to APHA, but questionable if followed exact method, so not sure about QA criteria"]
["Freshwater/metal/non- plant","159-3",64,0.64,"","Philodina acuticornis","mixed age","96 h","EC50","Immobility","14800 (total?)","Rotifer","Rotifera","Eurotatoria","Freshwater","20°C","Acute ",7.5,"N.A.","Buikema Jr A, See C and Cairns Jr J (1977). Rotifer sensitivity to combinations of inorganic water pollutants. Bulletin (Virginia Polytechnic Institute and State University. Water Resources Research Center), 92, 42. ","159","Note - refers to APHA, but questionable if followed exact method, so not sure about QA criteria"]
["Freshwater/metal/non- plant","160-1",62,0.62,"","Oryzias latipes","embryo","7 d","NOEC","Mortality","48600 (total)","Fish","Chordata","Actinopterygii","Embryo rearing medium","26°C","Chronic",7.2,"N.A.","Chen P-J, Wu W-L and Wu KC-W (2013). The zerovalent iron nanoparticle causes higher developmental toxicity than its oxidation products in early life stages of medaka fish. Water Research, 47(12), 3899-3909. ","160"]
["Freshwater/metal/non- plant","160-2",66,0.66,"","Oryzias latipes","embryo","7 d","LOEC","heart rate","25600 (total)","Fish","Chordata","Actinopterygii","Embryo rearing medium","26°C","Chronic",7.2,"N.A.","Chen P-J, Wu W-L and Wu KC-W (2013). The zerovalent iron nanoparticle causes higher developmental toxicity than its oxidation products in early life stages of medaka fish. Water Research, 47(12), 3899-3909. ","160"]
["Freshwater/metal/non- plant","160-3",66,0.66,"","Oryzias latipes","embryo","7 d","LOEC","hatching time","25600 (total)","Fish","Chordata","Actinopterygii","Embryo rearing medium","26°C","Chronic",7.2,"N.A.","Chen P-J, Wu W-L and Wu KC-W (2013). The zerovalent iron nanoparticle causes higher developmental toxicity than its oxidation products in early life stages of medaka fish. Water Research, 47(12), 3899-3909. ","160"]
["Freshwater/metal/non- plant","161-1",66,0.66,"","Oryzias latipes","larvae","14 days","NOEC","Mortality","25000 (total)","Fish","Chordata","Actinopterygii","Embryo rearing medium","26°C","Chronic",7.2,"N.A.","Chen PJ, Su CH, Tseng CY, Tan SW and Cheng CH (2011). Toxicity assessments of nanoscale zerovalent iron and its oxidation products in medaka (Oryzias latipes) fish. Marine Pollution Bulletin, 63(5-12), 339-346. ","161"]
["Freshwater/metal/non- plant","162-1",72,0.72,"","Oryzias latipes","larvae","72 h","NOEC","Mortality","24800 (total)","Fish","Chordata","Actinopterygii","Embryo rearing medium","26°C","Acute ",7.2,"N.A.","Chen PJ, Tan SW and Wu WL (2012). Stabilization or Oxidation of Nanoscale Zerovalent Iron at Environmentally Relevant Exposure Changes Bioavailability and Toxicity in Medaka Fish. Environmental Science & Technology, 46(15), 8431-8439. ","162"]
["Freshwater/metal/non- plant","163-1",96,0.96,"","Ceriodaphnia dubia","neonate","7 d","EC50","Reproduction","2012 (total)","Microinvertebrate","Arthropoda","Branchiopoda","EPA media","25°C","Chronic",6.3,"N.A.","CIMM (ed.) (2011) Iron (III) sulfate hydrate: Ceriodaphnia dubia survival and reproduction test at pH 6.3. Chilean Mining and Metallurgy Research Center (CIMM). ","163"]
["Freshwater/metal/non- plant","163-2",96,0.96,"","Ceriodaphnia dubia","neonate","7 d","EC10","Reproduction","570 (total)","Microinvertebrate","Arthropoda","Branchiopoda","EPA media","25°C","Chronic",6.3,"N.A.","CIMM (ed.) (2011) Iron (III) sulfate hydrate: Ceriodaphnia dubia survival and reproduction test at pH 6.3. Chilean Mining and Metallurgy Research Center (CIMM). ","163"]
["Freshwater/metal/non- plant","163-3",96,0.96,"","Ceriodaphnia dubia","neonate","7 d","EC10","Reproduction","1133 (total)","Microinvertebrate","Arthropoda","Branchiopoda","EPA media","25°C","Chronic",6.3,"N.A.","CIMM (ed.) (2011) Iron (III) sulfate hydrate: Ceriodaphnia dubia survival and reproduction test at pH 6.3. Chilean Mining and Metallurgy Research Center (CIMM). ","163"]
["Freshwater/metal/non- plant","163-4",96,0.96,"","Ceriodaphnia dubia","neonate","7 d","EC10","Reproduction","123 (dissolved)","Microinvertebrate","Arthropoda","Branchiopoda","EPA media","25°C","Chronic",6.3,"N.A.","CIMM (ed.) (2011) Iron (III) sulfate hydrate: Ceriodaphnia dubia survival and reproduction test at pH 6.3. Chilean Mining and Metallurgy Research Center (CIMM). ","163"]
["Freshwater/metal/non- plant","163-5",96,0.96,"","Ceriodaphnia dubia","neonate","7 d","EC50","Reproduction","6344 (total)","Microinvertebrate","Arthropoda","Branchiopoda","EPA media","25°C","Chronic",6.3,"N.A.","CIMM (ed.) (2011) Iron (III) sulfate hydrate: Ceriodaphnia dubia survival and reproduction test at pH 6.3. Chilean Mining and Metallurgy Research Center (CIMM). ","163"]
["Ptabluatedapers not assessed/:","163-6",96,0.96,"","Ceriodaphnia dubia","neonate","7 d","EC50","Reproduction","<281 (dissolved)","Microinvertebrate","Arthropoda","Branchiopoda","EPA media","25°C","Chronic",6.3,"N.A.","CIMM (ed.) (2011) Iron (III) sulfate hydrate: Ceriodaphnia dubia survival and reproduction test at pH 6.3. Chilean Mining and Metallurgy Research Center (CIMM). ","163"]
["Freshwater/metal/non- plant","163-7",96,0.96,"","Ceriodaphnia dubia","neonate","7 d","EC50","Reproduction","2283 (total)","Microinvertebrate","Arthropoda","Branchiopoda","EPA media","25°C","Chronic",6.3,"N.A.","CIMM (ed.) (2011) Iron (III) sulfate hydrate: Ceriodaphnia dubia survival and reproduction test at pH 6.3. Chilean Mining and Metallurgy Research Center (CIMM). ","163"]
["Freshwater/metal/non- plant","163-8",96,0.96,"","Ceriodaphnia dubia","neonate","7 d","EC10","Reproduction","1186 (total)","Microinvertebrate","Arthropoda","Branchiopoda","EPA media","25°C","Chronic",6.3,"N.A.","CIMM (ed.) (2011) Iron (III) sulfate hydrate: Ceriodaphnia dubia survival and reproduction test at pH 6.3. Chilean Mining and Metallurgy Research Center (CIMM). ","163"]
["Freshwater/metal/non- plant","163-9",96,0.96,"","Ceriodaphnia dubia","neonate","7 d","EC50","Reproduction","7486 (total)","Microinvertebrate","Arthropoda","Branchiopoda","EPA media","25°C","Chronic",6.3,"N.A.","CIMM (ed.) (2011) Iron (III) sulfate hydrate: Ceriodaphnia dubia survival and reproduction test at pH 6.3. Chilean Mining and Metallurgy Research Center (CIMM). ","163"]
["Freshwater/metal/non- plant","163-10",96,0.96,"","Ceriodaphnia dubia","neonate","7 d","EC10","Reproduction","6035 (total)","Microinvertebrate","Arthropoda","Branchiopoda","EPA media","25°C","Chronic",6.3,"N.A.","CIMM (ed.) (2011) Iron (III) sulfate hydrate: Ceriodaphnia dubia survival and reproduction test at pH 6.3. Chilean Mining and Metallurgy Research Center (CIMM). ","163"]
["Freshwater/metal/non- plant","163-11",96,0.96,"","Ceriodaphnia dubia","neonate","7 d","EC50","Reproduction","2256 (total)","Microinvertebrate","Arthropoda","Branchiopoda","EPA media","25°C","Chronic",6.3,"N.A.","CIMM (ed.) (2011) Iron (III) sulfate hydrate: Ceriodaphnia dubia survival and reproduction test at pH 6.3. Chilean Mining and Metallurgy Research Center (CIMM). ","163"]
["Freshwater/metal/non- plant","163-12",96,0.96,"","Ceriodaphnia dubia","neonate","7 d","EC10","Reproduction","919 (total)","Microinvertebrate","Arthropoda","Branchiopoda","EPA media","25°C","Chronic",6.3,"N.A.","CIMM (ed.) (2011) Iron (III) sulfate hydrate: Ceriodaphnia dubia survival and reproduction test at pH 6.3. Chilean Mining and Metallurgy Research Center (CIMM). ","163"]
["Freshwater/metal/non- plant","163-13",96,0.96,"","Ceriodaphnia dubia","neonate","7 d","EC50","Reproduction","3921 (total)","Microinvertebrate","Arthropoda","Branchiopoda","EPA media","25°C","Chronic",6.3,"N.A.","CIMM (ed.) (2011) Iron (III) sulfate hydrate: Ceriodaphnia dubia survival and reproduction test at pH 6.3. Chilean Mining and Metallurgy Research Center (CIMM). ","163"]
["Freshwater/metal/non- plant","163-14",96,0.96,"","Ceriodaphnia dubia","neonate","7 d","EC10","Reproduction","1776 (total)","Microinvertebrate","Arthropoda","Branchiopoda","EPA media","25°C","Chronic",6.3,"N.A.","CIMM (ed.) (2011) Iron (III) sulfate hydrate: Ceriodaphnia dubia survival and reproduction test at pH 6.3. Chilean Mining and Metallurgy Research Center (CIMM). ","163"]
["Freshwater/metal/non- plant","164-1",69,0.69,"","Daphnia magna","neonate","48 h","LC50","Immobility","9600 (total)","Microinvertebrate","Arthropoda","Branchiopoda","Unfiltered Lake Superior water","18°C","Acute ",7.7,"N.A.","Biesinger KE and Christensen GM (1972). Effects of various metals on survival, growth, reproduction, and metabolism of daphnia-magna.Journal of the Fisheries Research Board of Canada, 29(12), 1691-1700. ","164","No QA acceptability criteria, but was used in EPRI as only chronic values for iron. They assessed it as meeting with current USEPA guidelines."]
["Freshwater/metal/non- plant","164-2",74,0.74,"","Daphnia magna","neonate","21 d","EC16","Reproduction","4380 (total)","Microinvertebrate","Arthropoda","Branchiopoda","Unfiltered Lake Superior water","18°C","Chronic",7.7,"N.A.","Biesinger KE and Christensen GM (1972). Effects of various metals on survival, growth, reproduction, and metabolism of daphnia-magna.Journal of the Fisheries Research Board of Canada, 29(12), 1691-1700. ","164","No QA acceptability criteria, but was used in EPRI as only chronic values for iron. They assessed it as meeting with current USEPA guidelines."]
["Freshwater/metal/non- plant","164-3",74,0.74,"","Daphnia magna","neonate","21 d","EC50","Reproduction","5200 (total)","Microinvertebrate","Arthropoda","Branchiopoda","Unfiltered Lake Superior water","18°C","Chronic",7.7,"N.A.","Biesinger KE and Christensen GM (1972). Effects of various metals on survival, growth, reproduction, and metabolism of daphnia-magna.Journal of the Fisheries Research Board of Canada, 29(12), 1691-1700. ","164","No QA acceptability criteria, but was used in EPRI as only chronic values for iron. They assessed it as meeting with current USEPA guidelines."]
["Freshwater/metal/non- plant","164-4",69,0.69,"","Daphnia magna","neonate","21 d","EC50","Immobility","5900 (total)","Microinvertebrate","Arthropoda","Branchiopoda","Unfiltered Lake Superior water","18°C","Chronic",7.7,"N.A.","Biesinger KE and Christensen GM (1972). Effects of various metals on survival, growth, reproduction, and metabolism of daphnia-magna.Journal of the Fisheries Research Board of Canada, 29(12), 1691-1700. ","164","No QA acceptability criteria, but was used in EPRI as only chronic values for iron. They assessed it as meeting with current USEPA guidelines."]
["Freshwater/metal/non- plant","165-1",74,0.74,"","Salmo trutta","fingerlings","96 h","LC50","Mortality","47,000 (total)","Fish","Chordata","Actinopterygii","Freshwater","15°C","Acute ",6.2,"N.A.","Dalzell D and Macfarlane N (1999). The toxicity of iron to brown trout and effects on the gills: a comparison of two grades of iron sulphate. Journal of Fish Biology, 55(2), 301-315. ","165","No QA acceptability criteria",null,null,"Authors suggested effects due to precipitation."]
["Freshwater/metal/non- plant","165-2",74,0.74,"","Salmo trutta","fingerlings","96 h","LC50","Mortality","240 (dissolved)","Fish","Chordata","Actinopterygii","Freshwater","15°C","Acute ",6.2,"N.A.","Dalzell D and Macfarlane N (1999). The toxicity of iron to brown trout and effects on the gills: a comparison of two grades of iron sulphate. Journal of Fish Biology, 55(2), 301-315. ","165","No QA acceptability criteria"]
["Freshwater/metal/non- plant","166-1",58,0.57999999999999996,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonate","14 days","NOEC","Reproduction","128 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","21°C","Chronic",8,"N.A.","Dave G (1984). Effects of waterborne iron on growth, reproduction, survival and hemoglobin in daphnia-magna. Comparative Biochemistry and Physiology C-Pharmacology Toxicology & Endocrinology, 78(2), 433-438. ","166","Hardness = 250 mg/L"]
["Freshwater/metal/non- plant","166-2",58,0.57999999999999996,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonate","21 d","NOEC","Reproduction","128 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","21°C","Chronic",8,"N.A.","Dave G (1984). Effects of waterborne iron on growth, reproduction, survival and hemoglobin in daphnia-magna. Comparative Biochemistry and Physiology C-Pharmacology Toxicology & Endocrinology, 78(2), 433-438. ","166"]
["Freshwater/metal/non- plant","167-1",43,0.43,"The highest possible grade for this datum is acceptable.","Brachydanio rerio","embryo","16 days","N.A.","N.A.","no value","Fish","Chordata","Actinopterygii","Aged tap water","25°C","Chronic","N.A.","N.A.","Dave G (1985). The influence of pH on the toxicity of aluminum, cadmium, and iron to eggs and larvae of the zebrafish,< i> Brachydanio rerio</i>. Ecotoxicology and Environmental Safety, 10(2), 253-267. ","167"]
["Freshwater/metal/plant","168-1",26,0.26804123711340205,"The highest possible grade for this datum is acceptable.","Chlorella vulgaris","Not reported","3-4 months","NOEC","growth","3000 (nominal)","Microalga","Chlorophyta","Trebouxiophyceae","Freshwater","N.A.","Chronic","N.A.","N.A.","De Jong LED (1965). Tolerance of Chlorella vulgaris for metallic and non-metallic ions. Antonie Van Leeuwenhoek, 31(3), 301-313. ","168","No QA acceptability criteria",null,null,"note: pH of highest test conc of ~280 mg/L Fe(III) = pH 1.5"]
["Freshwater/metal/non- plant","169-1",70,0.7,"","Salmo gairdneri","sperm (dilution of 10^-2)","40 min","NOEC","fertilisation","80 (dissolved) 5000 (total)","Fish","Chordata","Actinopterygii","Freshwater","10°C","Acute",9,"N.A.","Billard R and Roubaud P (1985). The effect of metals and cyanide on fertilization in rainbow-trout (salmo-gairdneri). Water Research, 19(2), 209-214. ","169","No QA acceptability criteria"]
["Freshwater/metal/non- plant","169-2",70,0.7,"","Salmo gairdneri","sperm (dilution of 10^-1)","40 min","NOEC","fertilisation","<5 (dissolved) <1000 (total)","Fish","Chordata","Actinopterygii","Freshwater","10°C","Acute",9,"N.A.","Billard R and Roubaud P (1985). The effect of metals and cyanide on fertilization in rainbow-trout (salmo-gairdneri). Water Research, 19(2), 209-214. ","169","No QA acceptability criteria"]
["Freshwater/metal/non- plant","169-3",65,0.65,"","Salmo gairdneri","unfertilised egg","40 min","NOEC","fertilisation","80 (dissolved), 5000 (total)","Fish","Chordata","Actinopterygii","Freshwater","10°C","Acute",9,"N.A.","Billard R and Roubaud P (1985). The effect of metals and cyanide on fertilization in rainbow-trout (salmo-gairdneri). Water Research, 19(2), 209-214. ","169","No QA acceptability criteria"]
["Freshwater/metal/non- plant","170-1",56,0.56000000000000005,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonate","48 h","EC84","Immobility","8800 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","22 °C","Acute ","7.2-7.6","N.A.","Fjallborg B, Li B, Nilsson E and Dave G (2006). Toxicity identification evaluation of five metals performed with two organisms (Daphnia magna and Lactuca sativa). Archives of Environmental Contamination and Toxicology, 50(2), 196-204. ","170"]
["Freshwater/metal/non- plant","170-2",56,0.56000000000000005,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonate","48 h","EC84","Immobility","8800 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","22 °C","Acute ","8.0-8.2","N.A.","Fjallborg B, Li B, Nilsson E and Dave G (2006). Toxicity identification evaluation of five metals performed with two organisms (Daphnia magna and Lactuca sativa). Archives of Environmental Contamination and Toxicology, 50(2), 196-204. ","170"]
["Freshwater/metal/non- plant","171-1",67,0.67,"","Selvelinus fontinalis","Adult","96 h","LC50","Mortality","1750 (dissolved)","Fish","Chordata","Actinopterygii","Freshwater","11°C","Acute ",7,"N.A.","Decker C and Menendez R (1974). Acute toxicity of iron and aluminum to brook trout. Proceedings of the West Virginia Academy of Science, 46(2), 159-167. ","171"]
["Freshwater/metal/non- plant","171-2",67,0.67,"","Selvelinus fontinalis","Adult","96 h","LC50","Mortality","480 (dissolved)","Fish","Chordata","Actinopterygii","Freshwater","11°C","Acute ",6,"N.A.","Decker C and Menendez R (1974). Acute toxicity of iron and aluminum to brook trout. Proceedings of the West Virginia Academy of Science, 46(2), 159-167. ","171"]
["Freshwater/metal/non- plant","171-3",67,0.67,"","Selvelinus fontinalis","Adult","96 h","LC50","Mortality","410 (dissolved)","Fish","Chordata","Actinopterygii","Freshwater","11°C","Acute ",5.5,"N.A.","Decker C and Menendez R (1974). Acute toxicity of iron and aluminum to brook trout. Proceedings of the West Virginia Academy of Science, 46(2), 159-167. ","171"]
["Freshwater/metal/non- plant","172-1",66,0.66,"The highest possible grade for this datum is acceptable.","Ceriodaphnia dubia","neonate","48 h","EC50","Mortality","36690 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","N.R.","Acute ","7.0-7.3","N.A.","Fort DJ and Stover EL (1995). Impact of toxicities and potential interactions of flocculants and coagulant aids on whole effluent toxicity testing. Water Environment Research, 67(6), 921-925. ","172","mean of 2 definitive tests"]
["Freshwater/metal/non- plant","172-2",66,0.66,"The highest possible grade for this datum is acceptable.","Ceriodaphnia dubia","neonate","48 h","EC50","Mortality","30060 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","N.R.","Acute ","7.0-7.3","N.A.","Fort DJ and Stover EL (1995). Impact of toxicities and potential interactions of flocculants and coagulant aids on whole effluent toxicity testing. Water Environment Research, 67(6), 921-925. ","172","additional test done alongside a mixture test"]
["Freshwater/metal/non- plant","173-1",47,0.5,"","Leptophlebia marginata","larvae","30 days","NOEC","Mortality","20000 (total)","macroinvertebrate","Arthropoda","Insecta","stream water","11°C","Chronic",4.5,"N.A.","Gerhardt A (1992). Effects of subacute doses of iron (Fe) on Leptophelebia marginata (Insecta: Ephemeroptera). Freshwater Biology, 27, 79-84. ","173","No QA acceptability criteria",null,null,null,"Low quality paper"]
["Freshwater/metal/non- plant","173-2",47,0.5,null,"Leptophlebia marginata","larvae","30 days","NOEC","Mortality",">50000 (total)","macroinvertebrate","Arthropoda","Insecta","stream water","11°C","Chronic",7.5,"N.A.","Gerhardt A (1992). Effects of subacute doses of iron (Fe) on Leptophelebia marginata (Insecta: Ephemeroptera). Freshwater Biology, 27, 79-84. ","173","No QA acceptability criteria",null,null,null,"Low quality paper"]
["Freshwater/metal/non- plant","173-3",50,0.5,"","Leptophlebia marginata","larvae","30 days","NOEC","Immobility","<10000 (total)","macroinvertebrate","Arthropoda","Insecta","Freshwater","11°C","Chronic",4.5,"N.A.","Gerhardt A (1992). Effects of subacute doses of iron (Fe) on Leptophelebia marginata (Insecta: Ephemeroptera). Freshwater Biology, 27, 79-84. ","173","No QA acceptability criteria",null,null,null,"Low quality paper"]
["Freshwater/metal/non- plant","173-4",50,0.5,"","Leptophlebia marginata","larvae","30 days","NOEC","Immobility",">50000 (total)","macroinvertebrate","Arthropoda","Insecta","Freshwater","11°C","Chronic",7.5,"N.A.","Gerhardt A (1992). Effects of subacute doses of iron (Fe) on Leptophelebia marginata (Insecta: Ephemeroptera). Freshwater Biology, 27, 79-84. ","173","No QA acceptability criteria",null,null,null,"Low quality paper"]
["Freshwater/metal/non- plant","174-1",68,0.68,"","Leptophlebia marginata","nymph","96 h","LC50","Mortality","106300 (nominal)","macroinvertebrate","Arthropoda","Insecta","Freshwater","10°C","Acute ","6.1-6.7","N.A.","Gerhardt A (1994). Short term toxicity of iron (Fe) and lead (Pb) to the mayfly Leptophlebia marginata (L.) (Insecta) in relation to freshwater acidification. Hydrobiologia, 284(2), 157-168. ","174","No QA acceptability criteria",null,null,null,"measured values but did not use in stats"]
["Freshwater/metal/non- plant","174-2",68,0.68,"","Leptophlebia marginata","nymph","5 d","LC50","Mortality","106300 (nominal)","macroinvertebrate","Arthropoda","Insecta","Freshwater","10°C","Acute ","6.1-6.7","N.A.","Gerhardt A (1994). Short term toxicity of iron (Fe) and lead (Pb) to the mayfly Leptophlebia marginata (L.) (Insecta) in relation to freshwater acidification. Hydrobiologia, 284(2), 157-168. ","174","No QA acceptability criteria",null,null,null,"measured values but did not use in stats"]
["Freshwater/metal/non- plant","174-3",73,0.73,"","Leptophlebia marginata","nymph","96 h","EC50","escape behaviour","70000 (nominal)","macroinvertebrate","Arthropoda","Insecta","Freshwater","10°C","Acute ","6.1-6.7","N.A.","Gerhardt A (1994). Short term toxicity of iron (Fe) and lead (Pb) to the mayfly Leptophlebia marginata (L.) (Insecta) in relation to freshwater acidification. Hydrobiologia, 284(2), 157-168. ","174","No QA acceptability criteria",null,null,null,"measured values but did not use in stats",null,null,null,"not ecol rel?"]
["Freshwater/metal/non- plant","174-4",73,0.73,"","Leptophlebia marginata","nymph","5 d","EC50","escape behaviour","70000 (nominal)","macroinvertebrate","Arthropoda","Insecta","Freshwater","10°C","Acute ","6.1-6.7","N.A.","Gerhardt A (1994). Short term toxicity of iron (Fe) and lead (Pb) to the mayfly Leptophlebia marginata (L.) (Insecta) in relation to freshwater acidification. Hydrobiologia, 284(2), 157-168. ","174","No QA acceptability criteria",null,null,null,"measured values but did not use in stats",null,null,null,"not ecol rel?"]
["Freshwater/metal/non- plant","174-5",68,0.68,"","Leptophlebia marginata","nymph","96 h","LC50","Mortality","89500 (nominal)","macroinvertebrate","Arthropoda","Insecta","Freshwater","10°C","Acute ","4.4-4.6","N.A.","Gerhardt A (1994). Short term toxicity of iron (Fe) and lead (Pb) to the mayfly Leptophlebia marginata (L.) (Insecta) in relation to freshwater acidification. Hydrobiologia, 284(2), 157-168. ","174","No QA acceptability criteria",null,null,null,"measured values but did not use in stats"]
["Freshwater/metal/non- plant","174-6",68,0.68,"","Leptophlebia marginata","nymph","5 d","LC50","Mortality","65300 (nominal)","macroinvertebrate","Arthropoda","Insecta","Freshwater","10°C","Acute ","4.4-4.6","N.A.","Gerhardt A (1994). Short term toxicity of iron (Fe) and lead (Pb) to the mayfly Leptophlebia marginata (L.) (Insecta) in relation to freshwater acidification. Hydrobiologia, 284(2), 157-168. ","174","No QA acceptability criteria",null,null,null,"measured values but did not use in stats"]
["Freshwater/metal/non- plant","174-7",73,0.73,"","Leptophlebia marginata","nymph","96 h","EC50","escape behaviour","63900 (nominal)","macroinvertebrate","Arthropoda","Insecta","Freshwater","10°C","Acute ","4.4-4.6","N.A.","Gerhardt A (1994). Short term toxicity of iron (Fe) and lead (Pb) to the mayfly Leptophlebia marginata (L.) (Insecta) in relation to freshwater acidification. Hydrobiologia, 284(2), 157-168. ","174","No QA acceptability criteria",null,null,null,"measured values but did not use in stats",null,null,null,"not ecol rel?"]
["Freshwater/metal/non- plant","174-8",73,0.73,"","Leptophlebia marginata","nymph","5 d","EC50","escape behaviour","40200 (nominal)","macroinvertebrate","Arthropoda","Insecta","Freshwater","10°C","Acute ","4.4-4.6","N.A.","Gerhardt A (1994). Short term toxicity of iron (Fe) and lead (Pb) to the mayfly Leptophlebia marginata (L.) (Insecta) in relation to freshwater acidification. Hydrobiologia, 284(2), 157-168. ","174","No QA acceptability criteria",null,null,null,"measured values but did not use in stats",null,null,null,"not ecol rel?"]
["Freshwater/metal/non- plant","175-1",61,0.61,"The highest possible grade for this datum is acceptable.","Brachionus calyciflorus","<20 hours old","24 h","LC50","Mortality","230000 (nominal)","Microinvertebrate","Rotifera","Eurotatoria","Freshwater","25°C","Acute ",5.5,"N.A.","Calleja MC and Persoone G (1992). Cyst-based toxicity tests: 4. The potential of ecotoxicological tests for the prediction of acute toxicity in man as evaluated on the first ten chemicals of the MEIC programme. ATLA. Alternatives to Laboratory Animals, 20(3), 396-405. ","175","pH adjusted to 5.5 from initial low pH of ferrous sulphate solution"]
["Freshwater/metal/non- plant","175-2",60,0.6,"The highest possible grade for this datum is acceptable.","Streptocephalus proboscideus","Instar","24 h","LC50","Mortality","1420000 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","25°C","Acute ","Not Reported","N.A.","Calleja MC and Persoone G (1992). Cyst-based toxicity tests: 4. The potential of ecotoxicological tests for the prediction of acute toxicity in man as evaluated on the first ten chemicals of the MEIC programme. ATLA. Alternatives to Laboratory Animals, 20(3), 396-405. ","175"]
["Freshwater/metal/non- plant","176-1",35,0.35,"The highest possible grade for this datum is acceptable.","Asellus aquaticus","Not reported","96 h","LC50","Mortality","81080 (nominal)","macroinvertebrate","Arthropoda","Malocostraca","Freshwater","20°C","Acute ","Not Reported","N.A.","Furmanska M (1979). Effect of copper zinc and iron on the biotic components of aquatic ecosystems. Polskie Archiwum Hydrobiologii, 26(1-2), 213-220. ","176","No QA acceptability criteria"]
["Freshwater/metal/non- plant","176-2",35,0.35,"The highest possible grade for this datum is acceptable.","Lebistes reticulatus","Not reported","96 h","LC50","Mortality","73170 (nominal)","Fish","Chordata","Actinopterygii","Freshwater","20°C","Acute ","Not Reported","N.A.","Furmanska M (1979). Effect of copper zinc and iron on the biotic components of aquatic ecosystems. Polskie Archiwum Hydrobiologii, 26(1-2), 213-220. ","176","No QA acceptability criteria"]
["Freshwater/metal/non- plant","176-3",35,0.35,"The highest possible grade for this datum is acceptable.","Planorbarius","Not reported","96 h","LC50","Mortality","117180 (nominal)","Fish","Chordata","Actinopterygii","Freshwater","20°C","Acute ","Not Reported","N.A.","Furmanska M (1979). Effect of copper zinc and iron on the biotic components of aquatic ecosystems. Polskie Archiwum Hydrobiologii, 26(1-2), 213-220. ","176","No QA acceptability criteria"]
["Freshwater/metal/non- plant","177-1",70,0.7,"","Leptophlebia marginata","nymph","24 d","EC50","escape behaviour","19840 (total)","macroinvertebrate","Arthropoda","Insecta","Freshwater","N.R.","Chronic",4.5,"N.A.","Gerhardt A (1995). Joint and single toxicity of Cd and Fe related to metal uptake in the mayfly Leptophlebia marginata (L.) (Insecta). Hydrobiologia, 306(3), 229-240. ","177","No QA acceptability criteria",null,null,null,"low quality paper. ",null,null,null,"not ecol rel?"]
["Freshwater/metal/non- plant","178-1",68,0.68,"","Selvelinus fontinalis","Yearling","96 h","LC50","Mortality","1900 (? See notes)","Fish","Chordata","Actinopterygii","Artificial freshwater","14-15°C","Acute ","5.8-6.0","N.A.","Gonzalez RJ, Grippo RS and Dunson WA (1990).The disruption of sodium-balance in brook charr, Salvelinus-fontinalis (Mitchill), by manganese and iron. Journal of Fish Biology, 37(5), 765-774. ","178","No QA acceptability criteria. Synthetic water was 250 uM K+, Ca2+ and 450uM Na+ as chloride salts in deionized water",null,null,null,"low quality paper. ",null,"measured concentrations but no reported results for measurements. Very low on detail of toxicity tests."]
["Freshwater/metal/non- plant","178-2",68,0.68,"","Selvelinus fontinalis","Yearling","96 h","LC50","Mortality","900 (? See notes)","Fish","Chordata","Actinopterygii","Artificial freshwater","14-15°C","Acute ","5.8-6.0","N.A.","Gonzalez RJ, Grippo RS and Dunson WA (1990).The disruption of sodium-balance in brook charr, Salvelinus-fontinalis (Mitchill), by manganese and iron. Journal of Fish Biology, 37(5), 765-774. ","178","No QA acceptability criteria. Synthetic water was 250 uM K+, Ca2+ and 450uM Na+ as chloride salts in deionized water",null,null,null,"low quality paper. ",null,"measured concentrations but no reported results for measurements. Very low on detail of toxicity tests."]
["Freshwater/metal/non- plant","178-3",68,0.68,"","Selvelinus fontinalis","Yearling","96 h","LC50","Mortality","1300 (? See notes)","Fish","Chordata","Actinopterygii","Artificial freshwater","14-15°C","Acute ","5.8-6.0","N.A.","Gonzalez RJ, Grippo RS and Dunson WA (1990).The disruption of sodium-balance in brook charr, Salvelinus-fontinalis (Mitchill), by manganese and iron. Journal of Fish Biology, 37(5), 765-774. ","178","No QA acceptability criteria. Synthetic water was 250 uM K+, Ca2+ and 450uM Na+ as chloride salts in deionized water",null,null,null,"low quality paper. ",null,"measured concentrations but no reported results for measurements. Very low on detail of toxicity tests."]
["Freshwater/metal/non- plant","179-1",46,0.46,"The highest possible grade for this datum is acceptable.","Ceriodaphnia dubia","neonate","24 h","LC50","Mortality","46000 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","25°C","Acute ","reported to be 'low'","N.A.","Hockett JR and Mount DR (1996). Use of metal chelating agents to differentiate among sources of acute aquatic toxicity. Environmental Toxicology and Chemistry, 15(10), 1687-1693. ","179"]
["Freshwater/metal/plant","180-1",40,0.41237113402061853,"The highest possible grade for this datum is acceptable.","Pseudokirchneriella subcapitata","Not reported","96 h","LOEC","growth","5000 (nominal, Fe2+)","Microalga","Chlorophyta","Chlorophyceae","Freshwater","20°C","Chronic","N.R.","N.A.","Keller AA, Garner K, Miller RJ and Lenihan HS (2012). Toxicity of Nano-Zero Valent Iron to Freshwater and Marine Organisms. PLoS ONE, 7(8). ","180","No QA acceptability criteria"]
["Freshwater/metal/plant","180-2",40,0.41237113402061853,"The highest possible grade for this datum is acceptable.","Pseudokirchneriella subcapitata","Not reported","96 h","LOEC","growth","25000 (nominal, Fe3+)","Microalga","Chlorophyta","Chlorophyceae","Freshwater","20°C","Chronic","N.R.","N.A.","Keller AA, Garner K, Miller RJ and Lenihan HS (2012). Toxicity of Nano-Zero Valent Iron to Freshwater and Marine Organisms. PLoS ONE, 7(8). ","180","No QA acceptability criteria"]
["Freshwater/metal/non- plant","180-3",40,0.4,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonate","96 h","LOEC","Mortality","1000 (nominal Fe2+)","Fish","Chordata","Actinopterygii","Freshwater","N.R.","Acute ","Not Reported","N.A.","Keller AA, Garner K, Miller RJ and Lenihan HS (2012). Toxicity of Nano-Zero Valent Iron to Freshwater and Marine Organisms. PLoS ONE, 7(8). ","180"]
["Freshwater/metal/non- plant","180-4",40,0.4,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonate","96 h","LOEC","Mortality","15000 (nominal Fe3+)","Fish","Chordata","Actinopterygii","Freshwater","N.R.","Acute ","Not Reported","N.A.","Keller AA, Garner K, Miller RJ and Lenihan HS (2012). Toxicity of Nano-Zero Valent Iron to Freshwater and Marine Organisms. PLoS ONE, 7(8). ","180"]
["Freshwater/metal/non- plant","181-1",76,0.76,"The highest possible grade for this datum is acceptable.","Cherax destructor","juvenile","96 h","LC50","Mortality","51000 (nominal)","macroinvertebrate","Arthropoda","Malocostraca","Freshwater","21°C","Acute ","7 ± 1","N.A.","Khan S and Nugegoda D (2007). Sensitivity of juvenile freshwater crayfish Cherax destructor (Decapoda: Parastacidae) to trace metals. Ecotoxicology and Environmental Safety, 68(3), 463-469. ","181"]
["Freshwater/metal/non- plant","181-2",76,0.76,"The highest possible grade for this datum is acceptable.","Cherax destructor","juvenile","72 h","LC50","Mortality","71000 (nominal)","macroinvertebrate","Arthropoda","Malocostraca","Freshwater","21°C","Acute ","7 ± 1","N.A.","Khan S and Nugegoda D (2007). Sensitivity of juvenile freshwater crayfish Cherax destructor (Decapoda: Parastacidae) to trace metals. Ecotoxicology and Environmental Safety, 68(3), 463-469. ","181"]
["Freshwater/metal/non- plant","181-3",76,0.76,"The highest possible grade for this datum is acceptable.","Cherax destructor","juvenile","48 h","LC50","Mortality","117000 (nominal)","macroinvertebrate","Arthropoda","Malocostraca","Freshwater","21°C","Acute ","7 ± 1","N.A.","Khan S and Nugegoda D (2007). Sensitivity of juvenile freshwater crayfish Cherax destructor (Decapoda: Parastacidae) to trace metals. Ecotoxicology and Environmental Safety, 68(3), 463-469. ","181"]
["Freshwater/metal/non- plant","181-4",76,0.76,"The highest possible grade for this datum is acceptable.","Cherax destructor","juvenile","24 h","LC50","Mortality","177000 (nominal)","macroinvertebrate","Arthropoda","Malocostraca","Freshwater","21°C","Acute ","7 ± 1","N.A.","Khan S and Nugegoda D (2007). Sensitivity of juvenile freshwater crayfish Cherax destructor (Decapoda: Parastacidae) to trace metals. Ecotoxicology and Environmental Safety, 68(3), 463-469. ","181"]
["Freshwater/metal/non- plant","182-1",74,0.74,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","24 h","LC50","Mortality","112980 (nominal)","macroinvertebrate","Annelida","Clitellata","Freshwater","30°C","Acute ",7.6,"N.A.","Khangarot BS (1991). Toxicity of metals to a fresh-water tubificid worm, Tubifex-tubifex (muller).Bulletin of Environmental Contamination and Toxicology, 46(6), 906-912. ","182","pH measured in controls only"]
["Freshwater/metal/non- plant","182-2",74,0.74,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","48 h","LC50","Mortality","101840 (nominal)","macroinvertebrate","Annelida","Clitellata","Freshwater","30°C","Acute ",7.6,"N.A.","Khangarot BS (1991). Toxicity of metals to a fresh-water tubificid worm, Tubifex-tubifex (muller).Bulletin of Environmental Contamination and Toxicology, 46(6), 906-912. ","182"]
["Freshwater/metal/non- plant","182-3",74,0.74,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","96 h","LC50","Mortality","101840 (nominal)","macroinvertebrate","Annelida","Clitellata","Freshwater","30°C","Acute ",7.6,"N.A.","Khangarot BS (1991). Toxicity of metals to a fresh-water tubificid worm, Tubifex-tubifex (muller).Bulletin of Environmental Contamination and Toxicology, 46(6), 906-912. ","182"]
["Freshwater/metal/non- plant","183-1",81,0.81,"The highest possible grade for this datum is acceptable.","Cypris subglobosa","Not reported","48 h","EC50","Immobility","222700 (nominal)","Microinvertebrate","Arthropoda","Ostracoda","Freshwater","21°C","Acute ",7.6,"N.A.","Khangarot BS and Das S (2009). Acute toxicity of metals and reference toxicants to a freshwater ostracod, Cypris subglobosa Sowerby, 1840 and correlation to EC50 values of other test models. Journal of Hazardous Materials, 172(2-3), 641-649. ","183"]
["Freshwater/metal/non- plant","183-2",81,0.81,"The highest possible grade for this datum is acceptable.","Cypris subglobosa","Not reported","48 h","EC50","Immobility","115200 (nominal)","Microinvertebrate","Arthropoda","Ostracoda","Freshwater","21°C","Acute ",7.6,"N.A.","Khangarot BS and Das S (2009). Acute toxicity of metals and reference toxicants to a freshwater ostracod, Cypris subglobosa Sowerby, 1840 and correlation to EC50 values of other test models. Journal of Hazardous Materials, 172(2-3), 641-649. ","183"]
["Freshwater/metal/non- plant","184-1",75,0.75,"The highest possible grade for this datum is acceptable.","Daphnia magna","Not reported","24 h","LC50","Mortality","24500 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Well water","13°C","Acute ",7.6,"N.A.","Khangarot BS and Ray PK (1989). Investigation of correlation between physicochemical properties of metals and their toxicity to the water flea Daphnia-magna Straus. Ecotoxicology and Environmental Safety, 18(2), 109-120. ","184","hardness=240 mg/L, No QA acceptability criteria"]
["Freshwater/metal/non- plant","184-2",75,0.75,"The highest possible grade for this datum is acceptable.","Daphnia magna","Not reported","48 h","LC50","Mortality","7200 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Well water","13°C","Acute ",7.6,"N.A.","Khangarot BS and Ray PK (1989). Investigation of correlation between physicochemical properties of metals and their toxicity to the water flea Daphnia-magna Straus. Ecotoxicology and Environmental Safety, 18(2), 109-120. ","184","hardness=240 mg/L, No QA acceptability criteria"]
["Freshwater/metal/non- plant","185-1",75,0.75,"The highest possible grade for this datum is acceptable.","Rana hexadactyla","tadpoles","24 h","LC50","Mortality","24910 (nominal)","Amphibian","Chordata","Amphibia","Freshwater","15°C","Acute ",6.1,"N.A.","Khangarot BS, Sehgal A and Bhasin MK (1985). Man and biosphere - studies on the Sikkim Himalayas .5. Acute toxicity of selected heavy-metals on tadpoles of Rana-hexadactyla. Acta Hydrochimica Et Hydrobiologica, 13(2), 259-263. ","185","No QA acceptability criteria"]
["Freshwater/metal/non- plant","185-2",75,0.75,"The highest possible grade for this datum is acceptable.","Rana hexadactyla","tadpoles","48 h","LC50","Mortality","22390 (nominal)","Amphibian","Chordata","Amphibia","Freshwater","15°C","Acute ",6.1,"N.A.","Khangarot BS, Sehgal A and Bhasin MK (1985). Man and biosphere - studies on the Sikkim Himalayas .5. Acute toxicity of selected heavy-metals on tadpoles of Rana-hexadactyla. Acta Hydrochimica Et Hydrobiologica, 13(2), 259-263. ","185","No QA acceptability criteria"]
["Freshwater/metal/non- plant","185-3",75,0.75,"The highest possible grade for this datum is acceptable.","Rana hexadactyla","tadpoles","72 h","LC50","Mortality","19700 (nominal)","Amphibian","Chordata","Amphibia","Freshwater","15°C","Acute ",6.1,"N.A.","Khangarot BS, Sehgal A and Bhasin MK (1985). Man and biosphere - studies on the Sikkim Himalayas .5. Acute toxicity of selected heavy-metals on tadpoles of Rana-hexadactyla. Acta Hydrochimica Et Hydrobiologica, 13(2), 259-263. ","185","No QA acceptability criteria"]
["Freshwater/metal/non- plant","185-4",75,0.75,"The highest possible grade for this datum is acceptable.","Rana hexadactyla","tadpoles","96 h","LC50","Mortality","17620 (nominal)","Amphibian","Chordata","Amphibia","Freshwater","15°C","Acute ",6.1,"N.A.","Khangarot BS, Sehgal A and Bhasin MK (1985). Man and biosphere - studies on the Sikkim Himalayas .5. Acute toxicity of selected heavy-metals on tadpoles of Rana-hexadactyla. Acta Hydrochimica Et Hydrobiologica, 13(2), 259-263. ","185","No QA acceptability criteria"]
["Freshwater/metal/non- plant","186-1",43,0.43,"The highest possible grade for this datum is acceptable.","Bursaphelenchus xylophilus","Not reported","14 days","LC50","Immobility","20100 (nominal)","macroinvertebrate","Nematoda","Secernentea","Freshwater","25°C","Acute ","Not Reported","N.A.","Kobamoto N and Izumi Y (1984). The nematocidal effect of metal-ions on the pine wood nematode, Bursaphelenchus-xylophilus. Journal of Pesticide Science, 9(3), 527-529.","186","No QA acceptability criteria"]
["Freshwater/metal/non- plant","187-1",96,0.96,"","Pimephales promelas","larvae","7 d","NOEC","Biomass (dry weight)","128 (total) ","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187","Note: authors aimed to test at 0, 2, 4 mg DOC/L, but measured DOC 350 mL/L greater than these due to use of buffers to adjust pH."]
["Freshwater/metal/non- plant","187-2",96,0.96,"","Pimephales promelas","larvae","7 d","LOEC","Biomass (dry weight)","257 (total) ","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187","Also, dissolved Fe analysed but difficult to interpret."]
["Freshwater/metal/non- plant","187-3",96,0.96,"","Pimephales promelas","larvae","7 d","EC10","Biomass (dry weight)","192.1 (total) ","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-4",96,0.96,"","Pimephales promelas","larvae","7 d","EC20","Biomass (dry weight)","371.0 (total) ","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-5",96,0.96,"","Pimephales promelas","larvae","7 d","EC50","Biomass (dry weight)","2660 (total) ","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-6",93,0.93,"","Pimephales promelas","larvae","7 d","NOEC","Biomass (dry weight)","198.1 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-6",93,0.93,"","Pimephales promelas","larvae","7 d","LOEC","Biomass (dry weight)","387.7 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-7",93,0.93,"","Pimephales promelas","larvae","7 d","EC10","Biomass (dry weight)","293.6 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-8",93,0.93,"","Pimephales promelas","larvae","7 d","EC20","Biomass (dry weight)","1453.3 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-9",93,0.93,"","Pimephales promelas","larvae","7 d","EC50","Biomass (dry weight)","34725 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-10",96,0.96,"","Pimephales promelas","larvae","7 d","NOEC","Biomass (dry weight)","376.4 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-11",96,0.96,"","Pimephales promelas","larvae","7 d","LOEC","Biomass (dry weight)","751.3 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-12",96,0.96,"","Pimephales promelas","larvae","7 d","EC10","Biomass (dry weight)","316.0 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-13",96,0.96,"","Pimephales promelas","larvae","7 d","EC20","Biomass (dry weight)","584.9 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-14",96,0.96,"","Pimephales promelas","larvae","7 d","EC50","Biomass (dry weight)","1983.5 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-18",96,0.96,"","Pimephales promelas","larvae","7 d","NOEC","Biomass (dry weight)","385 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-19",96,0.96,"","Pimephales promelas","larvae","7 d","LOEC","Biomass (dry weight)","1914 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-20",96,0.96,"","Pimephales promelas","larvae","7 d","EC10","Biomass (dry weight)","332.3 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-21",96,0.96,"","Pimephales promelas","larvae","7 d","EC20","Biomass (dry weight)","857.6 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-22",96,0.96,"","Pimephales promelas","larvae","7 d","EC50","Biomass (dry weight)","5627 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-23",96,0.96,"","Pimephales promelas","larvae","7 d","NOEC","Biomass (dry weight)","3451 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-24",96,0.96,"","Pimephales promelas","larvae","7 d","LOEC","Biomass (dry weight)","8494.4 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-25",96,0.96,"","Pimephales promelas","larvae","7 d","EC10","Biomass (dry weight)","2782 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",6,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-26",93,0.93,"","Pimephales promelas","larvae","7 d","EC10","Biomass (dry weight)","532.7 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",8,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-28",96,0.96,"","Pimephales promelas","larvae","7 d","EC10","Biomass (dry weight)","27085 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",8,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-29",94,0.94,"","Pimephales promelas","larvae","7 d","EC10","Biomass (dry weight)","1704 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",8,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-30",96,0.96,"","Pimephales promelas","larvae","7 d","EC10","Biomass (dry weight)","15476 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",8,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","187-31",96,0.96,"","Pimephales promelas","larvae","7 d","EC10","Biomass (dry weight)","972.8 (total)","Fish","Chordata","Actinopterygii","Synthetic water","25°C","Chronic",8,"N.A.","Laboratory OSUAT (2013). Toxicity of Iron to the Fathead Minnow, Pimephales promelas, under varying pH, hardness, and dissolved organic carbon (DOC) conditions. Prepared by Oregon State University  Aquatic Toxicology Laboratory, Albany, Oregon, USA, February 2013.","187"]
["Freshwater/metal/non- plant","188-1",57,0.56999999999999995,"The highest possible grade for this datum is acceptable.","Daphnia pulex","neonate","24 h","EC50","Immobility","36858 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","OECD standard reference water","20°C","Acute ",7.6,"N.A.","Lilius H, Hastbacka T and Isomaa B (1995). A comparison of the toxicity of 30 reference chemicals to daphnia-magna and Daphnia-pulex. Environmental Toxicology and Chemistry, 14(12), 2085-2088. ","188"]
["Freshwater/metal/non- plant","189-1",56,0.56000000000000005,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonate","24 h","EC50","Immobility","5249 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","OECD standard reference water","21°C","Acute ",7.6,"N.A.","Lilius H, Isomaa B and Holmstrom T (1994). A comparison of the toxicity of 50 reference chemicals to freshly isolated rainbow-trout hepatocytes and Daphnia-magna. Aquatic Toxicology, 30(1), 47-60. ","189"]
["Freshwater/metal/non- plant","190-1",40,0.4,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonate","24 h","LC50","Mortality","16865 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","OECD standard reference water","N.R.","Acute ",5.5,"N.A.","Calleja MC, Persoone G and Geladi P (1993). The predictive potential of a battery of ecotoxicological tests for human acute toxicity, as evaluated with the 1st 50 MEIC chemicals. Atla-Alternatives to Laboratory Animals, 21(3), 330-349. ","190","Unsure of quality of this paper - very few dteails for methods. All referred to other papers."]
["Freshwater/metal/non- plant","191-1",69,0.69,"","Asellus aquaticus","6-10 mm males","52 h","LC50","Mortality","299800 (total Fe II)","macroinvertebrate","Arthropoda","Malocostraca","Freshwater","15°C","Acute ",4.5,"N.A.","Maltby L, Snart JOH and Calow P (1987). Acute toxicity tests on the fresh-water isopod, Asellus-aquaticus using FeSO4.7H2O, with special reference to techniques and the possibility of intraspecific variation. Environmental Pollution, 43(4), 271-279. ","191","Measured by a Hach kit - no precipitation (i.e. soluble - likely to be colloidal Fe due to use of citrate and tartrate buffers)"]
["Freshwater/metal/non- plant","191-2",69,0.69,"","Asellus aquaticus","6-10 mm males","55 h","LC50","Mortality","430500 (total Fe II)","macroinvertebrate","Arthropoda","Malocostraca","Freshwater","15°C","Acute ",6,"N.A.","Maltby L, Snart JOH and Calow P (1987). Acute toxicity tests on the fresh-water isopod, Asellus-aquaticus using FeSO4.7H2O, with special reference to techniques and the possibility of intraspecific variation. Environmental Pollution, 43(4), 271-279. ","191","Measured by a Hach kit - no precipitation (i.e. soluble - likely to be colloidal Fe due to use of citrate and tartrate buffers)"]
["Freshwater/metal/non- plant","192-1",67,0.67,"The highest possible grade for this datum is acceptable.","Gambusia affinis","Adult","96 h","LC50","Mortality","20000 (nominal)","Fish","Chordata","Actinopterygii","Freshwater","22-28","Acute ","6.9-7.5","N.A.","Mowbray DL (1988). Assessment of the biological impact of Ok Tedi mine tailings, cyanide and heavy metals through toxicity testing. In: Pernetta JC (ed.) Potential impacts of mining on the Fly River. UNEP, Nairobi, 45-74.","192","pH only measured in Controls"]
["Freshwater/metal/non- plant","192-2",67,0.67,"The highest possible grade for this datum is acceptable.","Gambusia affinis","Adult","96 h","LC50","Mortality","34000 (nominal)","Fish","Chordata","Actinopterygii","Freshwater","22-28","Acute ","6.9-7.5","N.A.","Mowbray DL (1988). Assessment of the biological impact of Ok Tedi mine tailings, cyanide and heavy metals through toxicity testing. In: Pernetta JC (ed.) Potential impacts of mining on the Fly River. UNEP, Nairobi, 45-74.","192","pH only measured in Controls"]
["Freshwater/metal/non- plant","192-3",67,0.67,"The highest possible grade for this datum is acceptable.","Caridina nilotica","22 mm","96 h","LC50","Mortality","8450 (nominal)","macroinvertebrate","Arthropoda","Malocostraca","Freshwater","22-28","Acute ","6.9-7.5","N.A.","Mowbray DL (1988). Assessment of the biological impact of Ok Tedi mine tailings, cyanide and heavy metals through toxicity testing. In: Pernetta JC (ed.) Potential impacts of mining on the Fly River. UNEP, Nairobi, 45-74.","192","pH only measured in Controls"]
["Freshwater/metal/non- plant","193-1",56,0.56000000000000005,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonate","48 h","EC50","Immobility","17 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","21°C","Acute ","Not Reported","N.A.","Oda S, Tatarazako N, Watanabe H, Morita M and Iguchi T (2006). Genetic differences in the production of male neonates in Daphnia magna exposed to juvenile hormone analogs. Chemosphere, 63(9), 1477-1484. ",193,"Assume units incorrect in paper? These tests were part of a suite of ref tox tests alongside a reproduction test. Good detail of media pH and phys-chem given for reproduction test. Assume same for Acute test, but not stated. REJECT paper."]
["Freshwater/metal/non- plant","193-2",56,0.56000000000000005,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonate","48 h","EC50","Immobility","19 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","21°C","Acute ","Not Reported","N.A.","Oda S, Tatarazako N, Watanabe H, Morita M and Iguchi T (2006). Genetic differences in the production of male neonates in Daphnia magna exposed to juvenile hormone analogs. Chemosphere, 63(9), 1477-1484. ","193","Assume units incorrect in paper? These tests were part of a suite of ref tox tests alongside a reproduction test. Good detail of media pH and phys-chem given for reproduction test. Assume same for Acute test, but not stated. REJECT paper."]
["Freshwater/metal/non- plant","193-3",56,0.56000000000000005,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonate","48 h","EC50","Immobility","19 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","21°C","Acute ","Not Reported","N.A.","Oda S, Tatarazako N, Watanabe H, Morita M and Iguchi T (2006). Genetic differences in the production of male neonates in Daphnia magna exposed to juvenile hormone analogs. Chemosphere, 63(9), 1477-1484. ","193","Assume units incorrect in paper? These tests were part of a suite of ref tox tests alongside a reproduction test. Good detail of media pH and phys-chem given for reproduction test. Assume same for Acute test, but not stated. REJECT paper."]
["Freshwater/metal/non- plant","194-1",72,0.72,"","Oryzias latipes","4 months old","96 h","LC50","Mortality","46600 (total)","Fish","Chordata","Actinopterygii","Freshwater","24°C","Acute ","3.5-7.42","N.A.","OECD (2004). Screening Information Data Sets (SIDS) Initial Assessment Report on Iron Dichloride, Organisation for Economic Cooperation and Development (OECD), Paris, France.","194","Day 0  pH 5.7 (100 mg/L), 7.42 (Control). By 96h, measured concs of 34400 and 62600 pH 4.1 and 3.5 respectively. Rest was >6.8. So at EC50, pH was around 4. And effects due to pH confirmed."]
["Freshwater/metal/plant","194-2",63,0.64948453608247425,"","Selenastrum capricornutum","N.A.","72 h","EC50","growth rate","6900 (total)","Microalga","Chlorophyta","Chlorophyceae","Freshwater","22-24","Chronic","7.5 at EC50","N.A.","OECD (2004). Screening Information Data Sets (SIDS) Initial Assessment Report on Iron Dichloride, Organisation for Economic Cooperation and Development (OECD), Paris, France.","194","pH 7.04-8.0 on Day 0, 4.74-7.64 on Day 2. 22 mg/L = pH 5.48 and 44 mg/L = pH 4.74 on Day 2. But at EC50, pH around 7.5 or less."]
["Freshwater/metal/plant","194-3",63,0.64948453608247425,"","Selenastrum capricornutum","N.A.","72 h","EC50","growth (biomass)","3800 (total)","Microalga","Chlorophyta","Chlorophyceae","Freshwater","22-24","Chronic","7.5 at EC50","N.A.","OECD (2004). Screening Information Data Sets (SIDS) Initial Assessment Report on Iron Dichloride, Organisation for Economic Cooperation and Development (OECD), Paris, France.","194","pH 7.04-8.0 on Day 0, 4.74-7.64 on Day 2. 22 mg/L = pH 5.48 and 44 mg/L = pH 4.74 on Day 2. But at EC50, pH around 7.5 or less."]
["Freshwater/metal/plant","194-3",63,0.64948453608247425,"","Selenastrum capricornutum","N.A.","72 h","NOEC","growth rate","3800 (total)","Microalga","Chlorophyta","Chlorophyceae","Freshwater","22-24","Chronic","7.5 at EC50","N.A.","OECD (2004). Screening Information Data Sets (SIDS) Initial Assessment Report on Iron Dichloride, Organisation for Economic Cooperation and Development (OECD), Paris, France.","194","pH 7.04-8.0 on Day 0, 4.74-7.64 on Day 2. 22 mg/L = pH 5.48 and 44 mg/L = pH 4.74 on Day 2. But at EC50, pH around 7.5 or less."]
["Freshwater/metal/plant","194-4",63,0.64948453608247425,"","Selenastrum capricornutum","N.A.","72 h","NOEC","growth (biomass)","1100 (total)","Microalga","Chlorophyta","Chlorophyceae","Freshwater","22-24","Chronic","7.5 at EC50","N.A.","OECD (2004). Screening Information Data Sets (SIDS) Initial Assessment Report on Iron Dichloride, Organisation for Economic Cooperation and Development (OECD), Paris, France.","194","pH 7.04-8.0 on Day 0, 4.74-7.64 on Day 2. 22 mg/L = pH 5.48 and 44 mg/L = pH 4.74 on Day 2. But at EC50, pH around 7.5 or less."]
["Freshwater/metal/non- plant","195-1",62,0.62,"The highest possible grade for this datum is acceptable.","Diaptomus forbesi","Not reported","48 h","LC50","Mortality","86500 (n ominal)","Microinvertebrate","Arthropoda","Maxillopoda","Freshwater","24-26","Acute ",7,"N.A.","Mullick S and Konar SK (1991). Combined effects of zinc, copper, iron and lead on plankton. Environment & Ecology, 9(1), 187-198. ","195","pH measured in controls only. Conc response was normal until 15 mg/L, when 52% effect observed. At concs of 30, 100, 125, 150, 175, 200 mg/L, mortality was 52, 58, 54, 76, 56%. Therefore, LC50 of 86 may not be accurate. Cls will go from 15 to 200 mg/L. Precipitate described at higher concentrations. Copepods swam to 'clear zone' to escape expposure in higher concentrations. Higher concentrations had quicker settling time for precipitate. Lower concentrations had colloidal Fe homogenous, ie no 'clear zone'."]
["Freshwater/metal/non- plant","195-2",62,0.62,"The highest possible grade for this datum is acceptable.","Diaptomus forbesi","Not reported","48 h","LC5","Mortality","0.00000075 (nominal, checked)","Microinvertebrate","Arthropoda","Maxillopoda","Freshwater","24-26","Acute ",7,"N.A.","Mullick S and Konar SK (1991). Combined effects of zinc, copper, iron and lead on plankton. Environment & Ecology, 9(1), 187-198. ","195","pH measured in controls only. Conc response was normal until 15 mg/L, when 52% effect observed. At concs of 30, 100, 125, 150, 175, 200 mg/L, mortality was 52, 58, 54, 76, 56%. Therefore, LC50 of 86 may not be accurate. Cls will go from 15 to 200 mg/L. Precipitate described at higher concentrations. Copepods swam to 'clear zone' to escape expposure in higher concentrations. Higher concentrations had quicker settling time for precipitate. Lower concentrations had colloidal Fe homogenous, ie no 'clear zone'."]
["Freshwater/metal/non- plant","196-1",50,0.5,"The highest possible grade for this datum is acceptable.","Tilapia mossambica","Fry","96 h","LC50","Mortality","83200 (nominal)","Fish","Chordata","Actinopterygii","Unchlorinated borehole water",27,"Acute ",7,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196","pHs adjusted using phosphate buffers. No comment on precipitation. Results for fish dubious, since LC5, LC50 and LC95 for each pH indicate flat level response at each pH."]
["Freshwater/metal/non- plant","196-2",50,0.5,"The highest possible grade for this datum is acceptable.","Tilapia mossambica","Fry","96 h","LC5","Mortality","73600 (nominal)","Fish","Chordata","Actinopterygii","Unchlorinated borehole water",27,"Acute ",7,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-3",50,0.5,"The highest possible grade for this datum is acceptable.","Tilapia mossambica","Fry","96 h","LC50","Mortality","119600 (nominal)","Fish","Chordata","Actinopterygii","Unchlorinated borehole water",27,"Acute ",6.5,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-4",50,0.5,"The highest possible grade for this datum is acceptable.","Tilapia mossambica","Fry","96 h","LC5","Mortality","110800 (nominal)","Fish","Chordata","Actinopterygii","Unchlorinated borehole water",27,"Acute ",6.5,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-5",50,0.5,"The highest possible grade for this datum is acceptable.","Tilapia mossambica","Fry","96 h","LC50","Mortality","118000 (nominal)","Fish","Chordata","Actinopterygii","Unchlorinated borehole water",27,"Acute ",8.5,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-6",50,0.5,"The highest possible grade for this datum is acceptable.","Tilapia mossambica","Fry","96 h","LC5","Mortality","99400 (nominal)","Fish","Chordata","Actinopterygii","Unchlorinated borehole water",27,"Acute ",8.5,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-7",45,0.45,"The highest possible grade for this datum is acceptable.","Cyclops viridis","Not reported","96 h","LC50","Mortality","33200 (nominal)","Microinvertebrate","Arthropoda","Maxillopoda","Unchlorinated borehole water",27,"Acute ",7,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196","Appears to be dose response curves for copepod and worm data, based on LC5, 50 and 95s. Data more trustworthy than fish, even though failed and not measured."]
["Freshwater/metal/non- plant","196-8",45,0.45,"The highest possible grade for this datum is acceptable.","Cyclops viridis","Not reported","96 h","LC5","Mortality","15600 (nominal)","Microinvertebrate","Arthropoda","Maxillopoda","Unchlorinated borehole water",27,"Acute ",7,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-9",45,0.45,"The highest possible grade for this datum is acceptable.","Cyclops viridis","Not reported","96 h","LC50","Mortality","35200 (nominal)","Microinvertebrate","Arthropoda","Maxillopoda","Unchlorinated borehole water",27,"Acute ",6.5,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-10",45,0.45,"The highest possible grade for this datum is acceptable.","Cyclops viridis","Not reported","96 h","LC5","Mortality","11800 (nominal)","Microinvertebrate","Arthropoda","Maxillopoda","Unchlorinated borehole water",27,"Acute ",6.5,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-11",45,0.45,"The highest possible grade for this datum is acceptable.","Cyclops viridis","Not reported","96 h","LC50","Mortality","36000 (nominal)","Microinvertebrate","Arthropoda","Maxillopoda","Unchlorinated borehole water",27,"Acute ",8.5,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-12",45,0.45,"The highest possible grade for this datum is acceptable.","Cyclops viridis","Not reported","96 h","LC5","Mortality","17400 (nominal)","Microinvertebrate","Arthropoda","Maxillopoda","Unchlorinated borehole water",27,"Acute ",8.5,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-13",45,0.45,"The highest possible grade for this datum is acceptable.","Branchiura sowerbyi","Not reported","96 h","LC50","Mortality","560000 (nominal)","macroinvertebrate","Annelida","Clitellata","Unchlorinated borehole water",27,"Acute ",7,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-14",45,0.45,"The highest possible grade for this datum is acceptable.","Branchiura sowerbyi","Not reported","96 h","LC5","Mortality","290000 (nominal)","macroinvertebrate","Annelida","Clitellata","Unchlorinated borehole water",27,"Acute ",7,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-15",45,0.45,"The highest possible grade for this datum is acceptable.","Branchiura sowerbyi","Not reported","96 h","LC50","Mortality","580000 (nominal)","macroinvertebrate","Annelida","Clitellata","Unchlorinated borehole water",27,"Acute ",6.5,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-16",45,0.45,"The highest possible grade for this datum is acceptable.","Branchiura sowerbyi","Not reported","96 h","LC5","Mortality","250000 (nominal)","macroinvertebrate","Annelida","Clitellata","Unchlorinated borehole water",27,"Acute ",6.5,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-17",45,0.45,"The highest possible grade for this datum is acceptable.","Branchiura sowerbyi","Not reported","96 h","LC50","Mortality","446000 (nominal)","macroinvertebrate","Annelida","Clitellata","Unchlorinated borehole water",27,"Acute ",8.5,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","196-18",45,0.45,"The highest possible grade for this datum is acceptable.","Branchiura sowerbyi","Not reported","96 h","LC5","Mortality","55000 (nominal)","macroinvertebrate","Annelida","Clitellata","Unchlorinated borehole water",27,"Acute ",8.5,"N.A.","Mukhopadhyay MK and Konar SK (1984). Toxicity of copper zinc and iron to fish Tilapia-mossambica plankton Cyclops-viridis and worm Brachium-sowerbyi. Geobios (Jodhpur), 11(5), 204-207. ","196"]
["Freshwater/metal/non- plant","197-1",75,0.75,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonate","48 h","LC50","Mortality","10220 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","13°C","Acute ",7.6,"N.A.","Khangarot BS and Ray PK (1988).The confirmation of a mammalian poison classification using a water flea (Daphnia-magna) screening method. Archiv fur Hydrobiologie, 113(3), 447-455. ","197","Authors state the pH range in all test solutions was 7.4-7.8."]
["Freshwater/metal/non- plant","198-1",47,0.47,"","Oncorhynchus mykiss","Not reported","96 h","LC50","not reported","15200 (total?)","Fish","Chordata","Actinopterygii","Freshwater","15°C","Acute ",">5","N.A.","Phippen BW, Horvath C, Nordin R and Nagpal N (2008). Ambient water quality guidelines for iron. February 28, 2008. Prepared for Science and Information Branch Water Stewardship Division Ministry of Environment. Ministry of Environment Province of British Columbia.","198","British Columbia report. Toxicity studies done by PESC specifically to derive Fe guideline from. Report does not however contain details of the testing, or results. So, although phys-chem and Fe measured beginning and end of test, how this data was used is not reported. I would assume the data to be of high quality, however I don't know if the values reported are total or dissolved. Also all test treatments were above a pH of 5 at test commencement, but no pH data beyond that. ",null,null,null,null,null,null,null,null,null,null,"Have requested more information from Monistry of Environment, BC."]
["Freshwater/metal/non- plant","198-2",47,0.47,"","Hyalella azteca","Not reported","96 h","LC50","not reported","3500  (total?)","Microinvertebrate","Arthropoda","Malocostraca","Freshwater","23°C","Acute ",">5","N.A.","Phippen BW, Horvath C, Nordin R and Nagpal N (2008). Ambient water quality guidelines for iron. February 28, 2008. Prepared for Science and Information Branch Water Stewardship Division Ministry of Environment. Ministry of Environment Province of British Columbia.","198",null,null,null,null,null,null,null,null,null,null,null,"Also, no QA criteria."]
["Freshwater/metal/non- plant","198-3",47,0.47,"","Daphnia magna","Not reported","21 d","NOEC","not reported","5300  (total?)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","20°C","Chronic",">5","N.A.","Phippen BW, Horvath C, Nordin R and Nagpal N (2008). Ambient water quality guidelines for iron. February 28, 2008. Prepared for Science and Information Branch Water Stewardship Division Ministry of Environment. Ministry of Environment Province of British Columbia.","198"]
["Freshwater/metal/plant","198-4",45,0.46391752577319589,"","Selenastrum capricornutum","Not reported","96 h","LC50","Not reported","3600 (total?)","Microalga","Chlorophyta","Chlorophyceae","Freshwater","not reported","Chronic",">5","N.A.","Phippen BW, Horvath C, Nordin R and Nagpal N (2008). Ambient water quality guidelines for iron. February 28, 2008. Prepared for Science and Information Branch Water Stewardship Division Ministry of Environment. Ministry of Environment Province of British Columbia.","198"]
["Freshwater/metal/non- plant","199-1",53,0.53,"","Chironomus riparius","larvae","96 h","NOEC","Growth (length)","1000 (total)","macroinvertebrate","Arthropoda","Insecta","Freshwater","20°C","Acute ",7.8,"N.A.","Postma JF, Kyed M and Admiraal W (1995). Site specific differentiation in metal tolerance in the midge Chironomus riparius (Diptera, Chironomidae). Hydrobiologia, 315(2), 159-165. ","199"]
["Freshwater/metal/non- plant","200-1",29,0.28999999999999998,"The highest possible grade for this datum is acceptable.","Daphnia longispina","Not reported","48 h","NOEC","Mortality","8000 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","20°C","Acute ","7-8","N.A.","Randall S, Harper D and Brierley B (1999). Ecological and ecophysiological impacts of ferric dosing in reservoirs. Hydrobiologia, 395, 355-364. ","200","8 mg/L NOEC described as having no particulate Fe, but 10 mg/L and greater = particulates and toxicity."]
["Freshwater/metal/non- plant","200-2",38,0.38,"The highest possible grade for this datum is acceptable.","Daphnia longispina","Not reported","48 h","ED50 (EC50)","Mortality","11480  (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","20°C","Acute ","7-8","N.A.","Randall S, Harper D and Brierley B (1999). Ecological and ecophysiological impacts of ferric dosing in reservoirs. Hydrobiologia, 395, 355-364. ","200"]
["Freshwater/metal/non- plant","200-3",33,0.33,"The highest possible grade for this datum is acceptable.","Daphnia longispina","Not reported","21 d","NOEC","Immobility","2000 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","20°C","Chronic","7-8","N.A.","Randall S, Harper D and Brierley B (1999). Ecological and ecophysiological impacts of ferric dosing in reservoirs. Hydrobiologia, 395, 355-364. ","200"]
["Freshwater/metal/non- plant","200-4",38,0.38,"The highest possible grade for this datum is acceptable.","Daphnia longispina","Not reported","21 d","ED50 (EC50)","Immobility","4490 (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","20°C","Chronic","7-8","N.A.","Randall S, Harper D and Brierley B (1999). Ecological and ecophysiological impacts of ferric dosing in reservoirs. Hydrobiologia, 395, 355-364. ","200"]
["Freshwater/metal/non- plant","201-1",78,0.78,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","96 h","EC50","Mortality","86090 (nominal)","macroinvertebrate","Annelida","Clitellata","Freshwater","15°C","Acute ",7.5,"N.A.","Rathore RS and Khangarot BS (2002). Effects of temperature on the sensitivity of sludge worm Tubifex tubifex Muller to selected heavy metals. Ecotoxicology and Environmental Safety, 53(1), 27-36. ","201","pH vlaues noted to have decreased in highesr concentrations of Zn and Pb, but nothing noted for Fe."]
["Freshwater/metal/non- plant","201-2",78,0.78,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","96 h","EC50","Mortality","125420 (nominal)","macroinvertebrate","Annelida","Clitellata","Freshwater","20°C","Acute ",7.5,"N.A.","Rathore RS and Khangarot BS (2002). Effects of temperature on the sensitivity of sludge worm Tubifex tubifex Muller to selected heavy metals. Ecotoxicology and Environmental Safety, 53(1), 27-36. ","201"]
["Freshwater/metal/non- plant","201-3",78,0.78,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","96 h","EC50","Mortality","95350 (nominal)","macroinvertebrate","Annelida","Clitellata","Freshwater","25°C","Acute ",7.5,"N.A.","Rathore RS and Khangarot BS (2002). Effects of temperature on the sensitivity of sludge worm Tubifex tubifex Muller to selected heavy metals. Ecotoxicology and Environmental Safety, 53(1), 27-36. ","201"]
["Freshwater/metal/non- plant","201-4",78,0.78,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","96 h","EC50","Mortality","71260 (nominal)","macroinvertebrate","Annelida","Clitellata","Freshwater","30°C","Acute ",7.5,"N.A.","Rathore RS and Khangarot BS (2002). Effects of temperature on the sensitivity of sludge worm Tubifex tubifex Muller to selected heavy metals. Ecotoxicology and Environmental Safety, 53(1), 27-36. ","201"]
["Freshwater/metal/non- plant","202-1",86,0.86,"","Lepomis macrochirus","Not reported","96 h","LC50","Mortality","20260 (total)","Fish","Chordata","Actinopterygii","Freshwater","22°C","Acute ","6.3 ± 1.5","N.A.","Birge, W.J., Black, J.A., Westerman, A.G., Short, T.M., Taylor, S.B., Bruser, D.M., Wallingford, E.D. (1985). Reccomendations on numerical values for regulating iron and chloride concentrations for the purpose of protecting warmwater species of aquatic life in the Commonwealth of Kentucky. Memorandum of Agreement No 5429, Kentucky Natural Resources and Environmental Protection Cabinet, Kentucky, USA. 73 pp.",202,"Note pH range was 6.3 ± 1.1. Effort was made to renew solutions every 12 h, so likley kept the solutions slightly acidic. No mention of pH effects on animals"]
["Freshwater/metal/non- plant","202-2",90,0.9,"","Pimephales promelas","Not reported","96 h","LC50","Mortality","21840 (total)","Fish","Chordata","Actinopterygii","Freshwater","22 °C","Acute ","6.7 ± 0.1","N.A.","Birge, W.J., Black, J.A., Westerman, A.G., Short, T.M., Taylor, S.B., Bruser, D.M., Wallingford, E.D. (1985). Reccomendations on numerical values for regulating iron and chloride concentrations for the purpose of protecting warmwater species of aquatic life in the Commonwealth of Kentucky. Memorandum of Agreement No 5429, Kentucky Natural Resources and Environmental Protection Cabinet, Kentucky, USA. 73 pp.",202,"Dissolved was also measured, but fluctuated significantly with renewals etc."]
["Freshwater/metal/non- plant","202-3",90,0.9,"","Lirceus fontinalis","Not reported","96 h","LC50","Mortality","80480 (total)","macroinvertebrate","Arthropoda","Malocostraca","Freshwater","21°C","Acute ","4.9 ± 2.0","N.A.","Birge, W.J., Black, J.A., Westerman, A.G., Short, T.M., Taylor, S.B., Bruser, D.M., Wallingford, E.D. (1985). Reccomendations on numerical values for regulating iron and chloride concentrations for the purpose of protecting warmwater species of aquatic life in the Commonwealth of Kentucky. Memorandum of Agreement No 5429, Kentucky Natural Resources and Environmental Protection Cabinet, Kentucky, USA. 73 pp.",202,"Note low pH"]
["Freshwater/metal/non- plant","202-4",90,0.9,"","Physa gyrina","Not reported","96 h","LC50","Mortality","12090 (total)","macroinvertebrate","Mollusca","Gastropoda","Freshwater","21°C","Acute ","7.2 ± 1.3","N.A.","Birge, W.J., Black, J.A., Westerman, A.G., Short, T.M., Taylor, S.B., Bruser, D.M., Wallingford, E.D. (1985). Reccomendations on numerical values for regulating iron and chloride concentrations for the purpose of protecting warmwater species of aquatic life in the Commonwealth of Kentucky. Memorandum of Agreement No 5429, Kentucky Natural Resources and Environmental Protection Cabinet, Kentucky, USA. 73 pp.",202]
["Freshwater/metal/non- plant","202-5",90,0.9,"","Daphnia pulex","First Instar stage","48 h","LC50","Mortality","12930 (total)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","21°C","Acute ","6.1 ± 1.4","N.A.","Birge, W.J., Black, J.A., Westerman, A.G., Short, T.M., Taylor, S.B., Bruser, D.M., Wallingford, E.D. (1985). Reccomendations on numerical values for regulating iron and chloride concentrations for the purpose of protecting warmwater species of aquatic life in the Commonwealth of Kentucky. Memorandum of Agreement No 5429, Kentucky Natural Resources and Environmental Protection Cabinet, Kentucky, USA. 73 pp.",202,"Note pH low"]
["Freshwater/metal/non- plant","202-6",90,0.9,"","Daphnia pulex","neonate","21 d","NOEC","Reproduction","700 (total)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","20°C","Chronic","7.6 ± 0.2","N.A.","Birge, W.J., Black, J.A., Westerman, A.G., Short, T.M., Taylor, S.B., Bruser, D.M., Wallingford, E.D. (1985). Reccomendations on numerical values for regulating iron and chloride concentrations for the purpose of protecting warmwater species of aquatic life in the Commonwealth of Kentucky. Memorandum of Agreement No 5429, Kentucky Natural Resources and Environmental Protection Cabinet, Kentucky, USA. 73 pp.",202,"Use this one for D pulex. Note: good pH range. Also measured endpoints of offspring/female and body length which also had same NOEC. "]
["Freshwater/metal/non- plant","202-7",90,0.9,"","Daphnia pulex","neonate","48 h","LC50","Mortality","10620 (total)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","20°C","Acute","6.4 ± 1.4","N.A.","Birge, W.J., Black, J.A., Westerman, A.G., Short, T.M., Taylor, S.B., Bruser, D.M., Wallingford, E.D. (1985). Reccomendations on numerical values for regulating iron and chloride concentrations for the purpose of protecting warmwater species of aquatic life in the Commonwealth of Kentucky. Memorandum of Agreement No 5429, Kentucky Natural Resources and Environmental Protection Cabinet, Kentucky, USA. 73 pp.",202,"Aged 0 test solution"]
["Freshwater/metal/non- plant","202-8",90,0.9,"","Daphnia pulex","neonate","48 h","LC50","Mortality","15810 (total, 3-d aged sol)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","20°C","Acute","5.9 ± 1.7","N.A.","Birge, W.J., Black, J.A., Westerman, A.G., Short, T.M., Taylor, S.B., Bruser, D.M., Wallingford, E.D. (1985). Reccomendations on numerical values for regulating iron and chloride concentrations for the purpose of protecting warmwater species of aquatic life in the Commonwealth of Kentucky. Memorandum of Agreement No 5429, Kentucky Natural Resources and Environmental Protection Cabinet, Kentucky, USA. 73 pp.","202"]
["Freshwater/metal/non- plant","202-9",90,0.9,"","Daphnia pulex","neonate","48 h","LC50","Mortality","17350 (total, 6-d aged sol)","Microinvertebrate","Arthropoda","Branchiopoda","Freshwater","20°C","Acute","6.1 ± 1.8","N.A.","Birge, W.J., Black, J.A., Westerman, A.G., Short, T.M., Taylor, S.B., Bruser, D.M., Wallingford, E.D. (1985). Reccomendations on numerical values for regulating iron and chloride concentrations for the purpose of protecting warmwater species of aquatic life in the Commonwealth of Kentucky. Memorandum of Agreement No 5429, Kentucky Natural Resources and Environmental Protection Cabinet, Kentucky, USA. 73 pp.","202"]
["Freshwater/metal/non- plant","202-10",85,0.85,"","Pimephales promelas","6-12h post fertilisation","33 d","NOEC","Mortality","320 (total)","Fish","Chordata","Actinopterygii","Freshwater","20°C","Chronic","7.6 ± 0.2","N.A.","Birge, W.J., Black, J.A., Westerman, A.G., Short, T.M., Taylor, S.B., Bruser, D.M., Wallingford, E.D. (1985). Reccomendations on numerical values for regulating iron and chloride concentrations for the purpose of protecting warmwater species of aquatic life in the Commonwealth of Kentucky. Memorandum of Agreement No 5429, Kentucky Natural Resources and Environmental Protection Cabinet, Kentucky, USA. 73 pp.","202","Use this value for P. promelas. Endpoints of wet weight (NOEC 1.6 mg/L), length (1.01 mg/L NOEC) not as sensitive as survival. Survival test done twice."]
["Freshwater/metal/non- plant","203-1",71,0.71,"","Chironomus riparius","Third Instar Larvae","48 h","NOEC","Mortality","200 000 µg/L (nominal - measured in stock)","Insect","Arthropoda","Insecta","Reconstituted softwater","22 °C","Acute ",4,"N.A.","Rousch JM, Simmons TW, Kerans BL and Smith BP (1997). Relative acute effects of low pH and high iron on the hatching and survival of the water mite (Arrenurus manubriator) and the aquatic insect (Chironomus riparius). Environmental Toxicology and Chemistry, 16(10), 2144-2150. ","203"]
["Freshwater/metal/non- plant","203-2",71,0.71,"","Arrenurus manubriator","Adult male","48 h","NOEC","Mortality","800 000 µg/L (nominal -measured in stock)","Mite","Arthropoda","Arachnida","Reconstituted softwater","22 °C","Acute ",4,"N.A.","Rousch JM, Simmons TW, Kerans BL and Smith BP (1997). Relative acute effects of low pH and high iron on the hatching and survival of the water mite (Arrenurus manubriator) and the aquatic insect (Chironomus riparius). Environmental Toxicology and Chemistry, 16(10), 2144-2150. ","203"]
["Freshwater/metal/non- plant","204-1",57,0.56999999999999995,"The highest possible grade for this datum is acceptable.","Asplanchna brightwellii","<24 h old","24 h","NOEC","Mortality","500 µg/L (nominal)","Rotifer","Rotifer","Eurotatoria","EPA media","25°C","Acute ",7.5,"N.A.","Santos-Medrano GE and Rico-Martínez R (2013). Lethal effects of five metals on the freshwater rotifers Asplanchna brigthwellii and Brachionus calyciflorus. Hidrobiologica, 23(1), 82-86. ","204"]
["Freshwater/metal/non- plant","204-2",57,0.56999999999999995,"The highest possible grade for this datum is acceptable.","Asplanchna brightwellii","<24 h old","24 h","LC50","Mortality","358 µg/L (nominal)","Rotifer","Rotifer","Eurotatoria","EPA media","25°C","Acute ",7.5,"N.A.","Santos-Medrano GE and Rico-Martínez R (2013). Lethal effects of five metals on the freshwater rotifers Asplanchna brigthwellii and Brachionus calyciflorus. Hidrobiologica, 23(1), 82-86. ","204"]
["Freshwater/metal/non- plant","204-3",57,0.56999999999999995,"The highest possible grade for this datum is acceptable.","Brachionus calyciflorus","<24 h old","24 h","NOEC","Mortality","100 µg/L (nominal)","Rotifer","Rotifer","Eurotatoria","EPA media","25°C","Acute ",7.5,"N.A.","Santos-Medrano GE and Rico-Martínez R (2013). Lethal effects of five metals on the freshwater rotifers Asplanchna brigthwellii and Brachionus calyciflorus. Hidrobiologica, 23(1), 82-86. ","204"]
["Freshwater/metal/non- plant","204-4",57,0.56999999999999995,"The highest possible grade for this datum is acceptable.","Brachionus calyciflorus","<24 h old","24 h","LC50","Mortality","232 µg/L (nominal)","Rotifer","Rotifer","Eurotatoria","EPA media","25°C","Acute ",7.5,"N.A.","Santos-Medrano GE and Rico-Martínez R (2013). Lethal effects of five metals on the freshwater rotifers Asplanchna brigthwellii and Brachionus calyciflorus. Hidrobiologica, 23(1), 82-86. ","204"]
["Freshwater/metal/non- plant","205-1",52,0.52,"The highest possible grade for this datum is acceptable.","Tetrahymena pyriformis","exponentially growing","9 h","IC50","Growth (relative generation time)","105000 µg/L (nominal)","Protozoan","Ciliophora","Oligohymenophorea","PPYS synthetic media","28°C","Chronic","Not Reported","N.A.","Sauvant MP, Pepin D, Bohatier J and Groliere CA (1995). MICROPLATE TECHNIQUE FOR SCREENING AND ASSESSING CYTOTOXICITY OF XENOBIOTICS WITH TETRAHYMENA-PYRIFORMIS. Ecotoxicology and Environmental Safety, 32(2), 159-165. ","205","Poor quality paper and failed Q7 on QAQC"]
["Freshwater/metal/non- plant","206-1",85,0.85,"","Nais elinguis","Adult","24 h","LC50","Mortality","337 (µg/L) (total)","Annelid","Annelida","Clitellata","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Nadzifah Y, Umirah NS and Ahmad AK (2012). Toxicity of Metals to an Aquatic Worm, Nais elinguis (Oligochaeta, Naididae). Research Journal of Environmental Toxicology, 6(4), 122-132.","206","Free swimming worm, hardness of 18 mg/L as CaCO3"]
["Freshwater/metal/non- plant","206-2",85,0.85,"","Nais elinguis","Adult","48 h","LC50","Mortality","239 (µg/L) (total)","Annelid","Annelida","Clitellata","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Nadzifah Y, Umirah NS and Ahmad AK (2012). Toxicity of Metals to an Aquatic Worm, Nais elinguis (Oligochaeta, Naididae). Research Journal of Environmental Toxicology, 6(4), 122-132.","206","Free swimming worm, hardness of 18 mg/L as CaCO3"]
["Freshwater/metal/non- plant","206-3",85,0.85,"","Nais elinguis","Adult","72 h","LC50","Mortality","176 (µg/L) (total)","Annelid","Annelida","Clitellata","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Nadzifah Y, Umirah NS and Ahmad AK (2012). Toxicity of Metals to an Aquatic Worm, Nais elinguis (Oligochaeta, Naididae). Research Journal of Environmental Toxicology, 6(4), 122-132.","206","Free swimming worm, hardness of 18 mg/L as CaCO3"]
["Freshwater/metal/non- plant","206-4",85,0.85,"","Nais elinguis","Adult","96 h","LC50","Mortality","123 (µg/L) (total)","Annelid","Annelida","Clitellata","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Nadzifah Y, Umirah NS and Ahmad AK (2012). Toxicity of Metals to an Aquatic Worm, Nais elinguis (Oligochaeta, Naididae). Research Journal of Environmental Toxicology, 6(4), 122-132.","206","Free swimming worm, hardness of 18 mg/L as CaCO3"]
["Freshwater/metal/non- plant","207-1",82,0.82,"","Stenocypris major","Adult","24 h","LC50","Mortality","911.14 µg/L (total)","Ostracod","Arthropoda","Ostracoda","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Ramle N-A and Abas A (2011). Toxicity of metals to a freshwater ostracod: Stenocypris major. Journal of toxicology, 2011. ","207"]
["Freshwater/metal/non- plant","207-2",82,0.82,"","Stenocypris major","Adult","48 h","LC50","Mortality","644.39 µg/L (total)","Ostracod","Arthropoda","Ostracoda","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Ramle N-A and Abas A (2011). Toxicity of metals to a freshwater ostracod: Stenocypris major. Journal of toxicology, 2011. ","207"]
["Freshwater/metal/non- plant","207-3",82,0.82,"","Stenocypris major","Adult","72 h","LC50","Mortality","521.24 µg/L (total)","Ostracod","Arthropoda","Ostracoda","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Ramle N-A and Abas A (2011). Toxicity of metals to a freshwater ostracod: Stenocypris major. Journal of toxicology, 2011. ","207"]
["Freshwater/metal/non- plant","207-4",78,0.78,"","Stenocypris major","Adult","96 h","LC50","Mortality","278.9 µg/L (total)","Ostracod","Arthropoda","Ostracoda","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Ramle N-A and Abas A (2011). Toxicity of metals to a freshwater ostracod: Stenocypris major. Journal of toxicology, 2011. ","207"]
["Freshwater/metal/non- plant","208-1",79,0.79,"","Duttaphrynus melanostictus","tadpoles","24 h","LC50","Mortality","1100 µg/L (total)","Amphibian","Chordata","Amphibia","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Nadzifah Y, Umirah NS and Ahmad AK (2012). Toxicity of metals to tadpoles of the common Sunda toad, Duttaphrynus melanostictus. Toxicological and Environmental Chemistry, 94(2), 364-376. ","208"]
["Freshwater/metal/non- plant","208-2",81,0.81,"","Duttaphrynus melanostictus","tadpoles","48 h","LC50","Mortality","600 µg/L (total)","Amphibian","Chordata","Amphibia","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Nadzifah Y, Umirah NS and Ahmad AK (2012). Toxicity of metals to tadpoles of the common Sunda toad, Duttaphrynus melanostictus. Toxicological and Environmental Chemistry, 94(2), 364-376. ","208"]
["Freshwater/metal/non- plant","208-3",81,0.81,"","Duttaphrynus melanostictus","tadpoles","72 h","LC50","Mortality","500 µg/L (total)","Amphibian","Chordata","Amphibia","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Nadzifah Y, Umirah NS and Ahmad AK (2012). Toxicity of metals to tadpoles of the common Sunda toad, Duttaphrynus melanostictus. Toxicological and Environmental Chemistry, 94(2), 364-376. ","208"]
["Freshwater/metal/non- plant","208-4",77,0.77,"","Duttaphrynus melanostictus","tadpoles","96 h","LC50","Mortality","400 µg/L (total)","Amphibian","Chordata","Amphibia","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Nadzifah Y, Umirah NS and Ahmad AK (2012). Toxicity of metals to tadpoles of the common Sunda toad, Duttaphrynus melanostictus. Toxicological and Environmental Chemistry, 94(2), 364-376. ","208"]
["Freshwater/metal/non- plant","209-1",74,0.74,"","Chironomus javanus","Fourth Instar Larvae","24 h","LC50","Mortality","3230 µg/L (total)","Insect","Arthropoda","Insecta","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Umirah NS and Abas A (2011). Toxicity of eight metals to Malaysian freshwater midge larvae Chironomus javanus (Diptera, Chironomidae). Toxicology and Industrial Health, 27(10), 879-886. ","209"]
["Freshwater/metal/non- plant","209-2",76,0.76,"","Chironomus javanus","Fourth Instar Larvae","48 h","LC50","Mortality","1650 µg/L (total)","Insect","Arthropoda","Insecta","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Umirah NS and Abas A (2011). Toxicity of eight metals to Malaysian freshwater midge larvae Chironomus javanus (Diptera, Chironomidae). Toxicology and Industrial Health, 27(10), 879-886. ","209"]
["Freshwater/metal/non- plant","209-3",74,0.74,"","Chironomus javanus","Fourth Instar Larvae","72 h","LC50","Mortality","830 µg/L (total)","Insect","Arthropoda","Insecta","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Umirah NS and Abas A (2011). Toxicity of eight metals to Malaysian freshwater midge larvae Chironomus javanus (Diptera, Chironomidae). Toxicology and Industrial Health, 27(10), 879-886. ","209"]
["Freshwater/metal/non- plant","209-4",76,0.76,"","Chironomus javanus","Fourth Instar Larvae","96 h","LC50","Mortality","620 µg/L (total)","Insect","Arthropoda","Insecta","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Umirah NS and Abas A (2011). Toxicity of eight metals to Malaysian freshwater midge larvae Chironomus javanus (Diptera, Chironomidae). Toxicology and Industrial Health, 27(10), 879-886. ","209"]
["Freshwater/metal/non- plant","210-1",83,0.83,"","Rasbora sumatrana ","Adult","24 h","LC50","Mortality","97100 µg/L (total)","Fish","Chordata","Actinopterygii","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Ramle NA and Abas A (2015). Comparative toxicity of eight metals on freshwater fish. Toxicology and Industrial Health, 31(9), 773-782. ","210"]
["Freshwater/metal/non- plant","210-2",81,0.81,"","Rasbora sumatrana ","Adult","48 h","LC50","Mortality","72100 µg/L (total)","Fish","Chordata","Actinopterygii","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Ramle NA and Abas A (2015). Comparative toxicity of eight metals on freshwater fish. Toxicology and Industrial Health, 31(9), 773-782. ","210"]
["Freshwater/metal/non- plant","210-3",81,0.81,"","Rasbora sumatrana ","Adult","72 h","LC50","Mortality","4310 µg/L (total)","Fish","Chordata","Actinopterygii","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Ramle NA and Abas A (2015). Comparative toxicity of eight metals on freshwater fish. Toxicology and Industrial Health, 31(9), 773-782. ","210"]
["Freshwater/metal/non- plant","210-4",81,0.81,"","Rasbora sumatrana ","Adult","96 h","LC50","Mortality","1710 µg/L (total)","Fish","Chordata","Actinopterygii","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Ramle NA and Abas A (2015). Comparative toxicity of eight metals on freshwater fish. Toxicology and Industrial Health, 31(9), 773-782. ","210"]
["Freshwater/metal/non- plant","210-5",79,0.79,"","Poecilia reticulata","Adult","24 h","LC50","Mortality","13940 µg/L (total)","Fish","Chordata","Actinopterygii","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Ramle NA and Abas A (2015). Comparative toxicity of eight metals on freshwater fish. Toxicology and Industrial Health, 31(9), 773-782. ","210"]
["Freshwater/metal/non- plant","210-6",81,0.81,"","Poecilia reticulata","Adult","48 h","LC50","Mortality","9180 µg/L (total)","Fish","Chordata","Actinopterygii","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Ramle NA and Abas A (2015). Comparative toxicity of eight metals on freshwater fish. Toxicology and Industrial Health, 31(9), 773-782. ","210"]
["Freshwater/metal/non- plant","210-7",81,0.81,"","Poecilia reticulata","Adult","72 h","LC50","Mortality","3070 µg/L (total)","Fish","Chordata","Actinopterygii","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Ramle NA and Abas A (2015). Comparative toxicity of eight metals on freshwater fish. Toxicology and Industrial Health, 31(9), 773-782. ","210"]
["Freshwater/metal/non- plant","210-8",81,0.81,"","Poecilia reticulata","Adult","96 h","LC50","Mortality","1460 µg/L (total)","Fish","Chordata","Actinopterygii","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Yakub N, Ramle NA and Abas A (2015). Comparative toxicity of eight metals on freshwater fish. Toxicology and Industrial Health, 31(9), 773-782. ","210"]
["Freshwater/metal/non- plant","211-1",79,0.79,"","Melanoides tuberculata","Adult","24 h","LC50","Mortality","42120 µg/L (total)","Snail","Mollusca","Gastropoda","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Nur-Amalina R and Nadzifah Y (2012). Toxicity of Metals to a Freshwater Snail, Melanoides tuberculata. Scientific World Journal. ","211"]
["Freshwater/metal/non- plant","211-2",81,0.81,"","Melanoides tuberculata","Adult","48 h","LC50","Mortality","21780 µg/L (total)","Snail","Mollusca","Gastropoda","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Nur-Amalina R and Nadzifah Y (2012). Toxicity of Metals to a Freshwater Snail, Melanoides tuberculata. Scientific World Journal. ","211"]
["Freshwater/metal/non- plant","211-3",81,0.81,"","Melanoides tuberculata","Adult","72 h","LC50","Mortality","13290 µg/L (total)","Snail","Mollusca","Gastropoda","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Nur-Amalina R and Nadzifah Y (2012). Toxicity of Metals to a Freshwater Snail, Melanoides tuberculata. Scientific World Journal. ","211"]
["Freshwater/metal/non- plant","211-4",81,0.81,"","Melanoides tuberculata","Adult","96 h","LC50","Mortality","8490 µg/L (total)","Snail","Mollusca","Gastropoda","Dechlorinated tap water","28 - 30 oC","Acute ",6.5,"N.A.","Shuhaimi-Othman M, Nur-Amalina R and Nadzifah Y (2012). Toxicity of Metals to a Freshwater Snail, Melanoides tuberculata. Scientific World Journal. ","211"]
["Freshwater/metal/non- plant","212-1",73,0.73,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","24 h","LC50","Mortality",">1000000 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",6.6,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","12 mg/L Hardness - benthic species"]
["Freshwater/metal/non- plant","212-2",73,0.73,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","48 h","LC50","Mortality","100000 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",6.6,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","12 mg/L Hardness  - benthic species"]
["Freshwater/metal/non- plant","212-3",77,0.77,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","72 h","LC50","Mortality","43220 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",6.6,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","12 mg/L Hardness  - benthic species"]
["Freshwater/metal/non- plant","212-4",77,0.77,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","96 h","LC50","Mortality","17800 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",6.6,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","12 mg/L Hardness  - benthic species"]
["Freshwater/metal/non- plant","212-5",77,0.77,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","24 h","LC50","Mortality","35010 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",7.3,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","45 mg/L Hardness  - benthic species"]
["Freshwater/metal/non- plant","212-6",77,0.77,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","48 h","LC50","Mortality","27790 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",7.3,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","45 mg/L Hardness - benthic species"]
["Freshwater/metal/non- plant","212-7",77,0.77,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","72 h","LC50","Mortality","26490 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",7.3,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","45 mg/L Hardness - benthic species"]
["Freshwater/metal/non- plant","212-8",77,0.77,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","96 h","LC50","Mortality","25130 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",7.3,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","45 mg/L Hardness - benthic species"]
["Freshwater/metal/non- plant","212-9",77,0.77,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","24 h","LC50","Mortality","68220 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",7.8,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","173 mg/L Hardness - benthic species"]
["Freshwater/metal/non- plant","212-10",77,0.77,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","48 h","LC50","Mortality","56490 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",7.8,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","173 mg/L Hardness - benthic species"]
["Freshwater/metal/non- plant","212-11",77,0.77,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","72 h","LC50","Mortality","37500 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",7.8,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","173 mg/L Hardness - benthic species"]
["Freshwater/metal/non- plant","212-12",77,0.77,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","96 h","LC50","Mortality","37500 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",7.8,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","173 mg/L Hardness - benthic species"]
["Freshwater/metal/non- plant","212-13",77,0.77,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","24 h","LC50","Mortality","119750 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",8.1999999999999993,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","305 mg/L Hardness - benthic species"]
["Freshwater/metal/non- plant","212-14",77,0.77,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","48 h","LC50","Mortality","119750 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",8.1999999999999993,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","305 mg/L Hardness - benthic species"]
["Freshwater/metal/non- plant","212-15",77,0.77,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","72 h","LC50","Mortality","108820 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",8.1999999999999993,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","305 mg/L Hardness - benthic species"]
["Freshwater/metal/non- plant","212-16",77,0.77,"The highest possible grade for this datum is acceptable.","Tubifex tubifex","Not reported","96 h","LC50","Mortality","108820 µg/L (nominal)","Annelid","Annelida","Clitellata","Synthetic water","20°C","Acute ",8.1999999999999993,"N.A.","Rathore RS and Khangarot BS (2003). Effects of water hardness and metal concentration on a freshwater Tubifex tubifex muller. Water, Air, and Soil Pollution, 142(1-4), 341-356. ","212","305 mg/L Hardness - benthic species"]
["Freshwater/metal/non- plant","213-1",53,0.53,"The highest possible grade for this datum is acceptable.","Daphnia magna","Not reported","24 h","EC50","Immobility","16000 µg/L (nominal)","Microinvertebrate","Arthropoda","Branchiopoda","ISO 6341 synthetic water","25°C","Acute ","Not Reported","N.A.","Sorvari J and Sillanpaa M (1996). Influence of metal complex formation on heavy metal and free EDTA and DTPA acute toxicity determined by Daphnia magna. Chemosphere, 33(6), 1119-1127. ","213","Weak reference "]
["Freshwater/metal/non- plant","214-1",48,0.48,"The highest possible grade for this datum is acceptable.","Margaritifera margaritifera","glochidia","48 h","NOEC","Mortality","500 µg/L (nominal)","Mussel","Mollusca","Bivalve","Unmodified River water","6 oC","Acute ",6.8,"N.A.","Taskinen J, Berg P, Saarinen-Valta M, Valila S, Maenpaa E, Myllynen K and Pakkala J (2011). Effect of pH, iron and aluminum on survival of early life history stages of the endangered freshwater pearl mussel, Margaritifera margaritifera. Toxicological and Environmental Chemistry, 93(9), 1764-1777.","214","Weak reference, pH of river water diluent reported - no hardness"]
["Freshwater/metal/non- plant","215-1",58,0.57999999999999996,"","Lecane quadridentata","neonate","48 h","NOEC","Immobility","10 µg/L (total)","Rotifer","Rotifer","Eurotatoria","EPA media","25°C","Acute ","Not Reported","N.A.","Torres Guzman F, Avelar Gonzalez FJ and Rico Martinez R (2010). Implementing Lecane quadridentata acute toxicity tests to assess the toxic effects of selected metals (Al, Fe and Zn). Ecotoxicology and Environmental Safety, 73(3), 287-295. ","215"]
["Freshwater/metal/non- plant","215-2",58,0.57999999999999996,"","Lecane quadridentata","neonate","48 h","LC50","Immobility","539 µg/L (total)","Rotifer","Rotifer","Eurotatoria","EPA media","25°C","Acute ","Not Reported","N.A.","Torres Guzman F, Avelar Gonzalez FJ and Rico Martinez R (2010). Implementing Lecane quadridentata acute toxicity tests to assess the toxic effects of selected metals (Al, Fe and Zn). Ecotoxicology and Environmental Safety, 73(3), 287-295. ","215"]
["Freshwater/metal/plant","216-1",50,0.51546391752577314,"The highest possible grade for this datum is acceptable.","Lemna minor","frond","96 h","EC50","Growth (frond number)","3700 µg/L (nominal)","Macrophyte","Tracheophyta","Liliopsida","Plant nutrient solution","27 oC","Acute ","not recorded","N.A.","Wang W (1986). Toxicity tests of aquatic pollutants by using common duckweed. Environmental Pollution Series B, Chemical and Physical, 11(1), 1-14. ","216","Weak paper, bioassay development but without a lot of QAQC"]
["Freshwater/metal/non- plant","217-1",64,0.64,"","Ephemerella subvaria","larvae","96 h","LC50","Mortality","320 µg/L (total initial)","Mayfly","Arthropoda","Insecta","Lake Superior tap water","18°C","Acute ","7.25 - 8.2","N.A.","Warnick SL and Bell HL (1969). The acute toxicity of some heavy metals to different species of aquatic insects. Journal Water Pollution Control Federation, 41(2P1), 280-&. ","217","Hardness = 44 mg/L. Physchem considered, metals measured, one of few papers on mayflies"]
["Freshwater/metal/non- plant","217-2",64,0.64,"","Acroneuria lycorias","larvae","9 d","LC50","Mortality","16000 ug/L (total)","macroinvertebrate","Arthropoda","Insecta","Lake Superior carbon filtered water","18°C","Chronic","7.25 - 7.7","N.A.","Warnick SL and Bell HL (1969). The acute toxicity of some heavy metals to different species of aquatic insects. Journal Water Pollution Control Federation, 41(2 PartI), 280-284. \n","217","Hardness = 44 mg/L. Physchem considered, metals measured, one of few papers on stoneflies"]
["Freshwater/metal/non- plant","217-3",64,0.64,"","Hydropsyche betteni","larvae","7 d","LC50","Mortality","16000 ug/L (total)","macroinvertebrate","Arthropoda","Insecta","Lake Superior carbon filtered water","18°C","Chronic","7.25 - 8.1","N.A.","Warnick SL and Bell HL (1969). The acute toxicity of some heavy metals to different species of aquatic insects. Journal Water Pollution Control Federation, 41(2 PartI), 280-284. \n","217","Hardness = 44 mg/L. Physchem considered, metals measured, one of few papers on caddisflies"]
["Freshwater/metal/non- plant","218-1",62,0.62,"","Pimephales promelas","3 months old","5 months","LC10","Mortality","9822 µg/L (total)","Fish","Chordata","Actinopterygii","Well water","24°C","Chronic","6.86 - 7.16","N.A.","Sykora JL, Smith EJ, Shapiro MA and Synak N (1972). Chronic effect of ferric hydroxide on certain species of aquatic animals. In Proc. 4th Symp. on Coal Mine Drainage Research, Mellon Institute, Pittsburgh, Pennsylvania. pp. 347-369.","218","Hardness = 139-238 mg/L.  Used raw data from the appendix to calculate this value in Toxcalc i.e. it was not reported in the paper.  DID NOT PASS QAQC Q7"]
["Freshwater/metal/non- plant","218-2",62,0.62,"","Pimephales promelas","3 months old","5 months","LC50","Mortality","35180 µg/L (total)","Fish","Chordata","Actinopterygii","Well water","24°C","Chronic","6.86 - 7.16","N.A.","Sykora JL, Smith EJ, Shapiro MA and Synak N (1972). Chronic effect of ferric hydroxide on certain species of aquatic animals. In Proc. 4th Symp. on Coal Mine Drainage Research, Mellon Institute, Pittsburgh, Pennsylvania. pp. 347-369.","218","Hardness = 139-238 mg/L.  Used raw data from the appendix to calculate this value in Toxcalc i.e. it was not reported in the paper.  DID NOT PASS QAQC Q7"]
["Freshwater/metal/non- plant","218-3",57,0.56999999999999995,"","Salvelinus fontinalis","3 months old","2 years","N.A.","Mortality","None calculated","Fish","Chordata","Actinopterygii","Well water","15°C","Chronic","7.0 - 7.3","N.A.","Sykora JL, Smith EJ, Shapiro MA and Synak N (1972). Chronic effect of ferric hydroxide on certain species of aquatic animals. In Proc. 4th Symp. on Coal Mine Drainage Research, Mellon Institute, Pittsburgh, Pennsylvania. pp. 347-369.\n","218","Hardness = 128 - 214 mg/L. DID NOT PASS QAQC Q7"]
["Freshwater/metal/non- plant","218-4",57,0.56999999999999995,"","Salvelinus fontinalis","3 months old","2 years","N.A.","Growth (length)","None calculated","Fish","Chordata","Actinopterygii","Well water","15°C","Chronic","7.0 - 7.3","N.A.","Sykora JL, Smith EJ, Shapiro MA and Synak N (1972). Chronic effect of ferric hydroxide on certain species of aquatic animals. In Proc. 4th Symp. on Coal Mine Drainage Research, Mellon Institute, Pittsburgh, Pennsylvania. pp. 347-369.\n","218","Hardness = 128 - 214 mg/L. DID NOT PASS QAQC Q7"]
["Freshwater/metal/non- plant","218-5",57,0.56999999999999995,"","Salvelinus fontinalis","3 months old","2 years","N.A.","Growth (weight)","None calculated","Fish","Chordata","Actinopterygii","Well water","15°C","Chronic","7.0 - 7.3","N.A.","Sykora JL, Smith EJ, Shapiro MA and Synak N (1972). Chronic effect of ferric hydroxide on certain species of aquatic animals. In Proc. 4th Symp. on Coal Mine Drainage Research, Mellon Institute, Pittsburgh, Pennsylvania. pp. 347-369.\n","218","Hardness = 128 - 214 mg/L. DID NOT PASS QAQC Q7"]
["Freshwater/metal/non- plant","218-6",57,0.56999999999999995,"","Gammarus minus","Adult","14 weeks","N.A.","Mortality","None calculated","macroinvertebrate","Arthropoda","Malacostraca","Well water","18°C","Chronic","7.1 - 7.6","N.A.","Sykora JL, Smith EJ, Shapiro MA and Synak N (1972). Chronic effect of ferric hydroxide on certain species of aquatic animals. In Proc. 4th Symp. on Coal Mine Drainage Research, Mellon Institute, Pittsburgh, Pennsylvania. pp. 347-369.\n","218","Hardness = 112 - 256 mg/L. DID NOT PASS QAQC  Q7"]
["Freshwater/metal/non- plant","218-7",47,0.47,"","Cheumatopsyche","Third Instar Larvae","N.R.","NR","Emergence","None calculated","Insect","Arthropoda","Insecta","Well water","18°C","Chronic","7.1 - 7.6","N.A.","Sykora JL, Smith EJ, Shapiro MA and Synak N (1972). Chronic effect of ferric hydroxide on certain species of aquatic animals. In Proc. 4th Symp. on Coal Mine Drainage Research, Mellon Institute, Pittsburgh, Pennsylvania. pp. 347-369.\n","218","Hardness = 112 - 256 mg/L. DID NOT PASS QAQC  Q7"]
["Freshwater/metal/non- plant","219-1",61,0.61,"","Salvelinus fontinalis","3 months old","35 weeks","NOEC","Growth (length)","13420 µg/L (total)","Fish","Chordata","Actinopterygii","Well water","8 - 21.6 oC","Chronic","6.5 - 7.9","N.A.","Sykora JL, Smith EJ and Synak M (1972). Effect of lime neutralized iron hydroxide suspensions on juvenile brook trout (salvelinus fontinalis, mitchill). Water Research, 6(8), 935-950. \n","219","Hardnesss  = 120 - 236 mg/L. DID NOT PASS QAQC Q7"]
["Freshwater/metal/non- plant","220-1",57,0.56999999999999995,"","Pimephales promelas","3 months old","12 months","NOEC","Growth (length)","1500 ug/L (total)","Fish","Chordata","Actinopterygii","Well water","22.6 - 24.8","Chronic","6.9 - 7.2","N.A.","Smith EJ, Sykora JL and Shapiro MA (1973). Effect of Lime Neutralized Iron Hydroxide Suspensions on Survival, Growth, and Reproduction of the Fathead Minnow (Pimephales promelas). Journal of the Fisheries Research Board of Canada, 30(8), 1147-1153. \n","220","Hardness  = 139 - 238 mg/L gradient . DID NOT PASS QAQC Q7"]
["Freshwater/metal/non- plant","220-2",57,0.56999999999999995,"","Pimephales promelas","3 months old","5 months","NR","Mortality","effects at 25 mg/L after 1 month and 6 mg/L after 5 months","Fish","Chordata","Actinopterygii","Well water","22.6 - 24.8","Chronic","6.9 - 7.2","N.A.","Smith EJ, Sykora JL and Shapiro MA (1973). Effect of Lime Neutralized Iron Hydroxide Suspensions on Survival, Growth, and Reproduction of the Fathead Minnow (Pimephales promelas). Journal of the Fisheries Research Board of Canada, 30(8), 1147-1153. \n","220","Hardness  = 139 - 238 mg/L gradient . DID NOT PASS QAQC Q7"]
["Freshwater/metal/non- plant","220-3",57,0.56999999999999995,"","Pimephales promelas","3 months old","12 months","NR","hatching success","effects at 2 mg/L (total Fe)","Fish","Chordata","Actinopterygii","Well water","22.6 - 24.8","Chronic","6.9 - 7.2","N.A.","Smith EJ, Sykora JL and Shapiro MA (1973). Effect of Lime Neutralized Iron Hydroxide Suspensions on Survival, Growth, and Reproduction of the Fathead Minnow (Pimephales promelas). Journal of the Fisheries Research Board of Canada, 30(8), 1147-1153. \n","220","Hardness  = 139 - 238 mg/L gradient . DID NOT PASS QAQC Q7"]
["Freshwater/metal/non- plant","220-4",57,0.56999999999999995,"","Pimephales promelas","30-d post hatch","30 days","NR","Mortality","effects at 2 mg/L (total Fe)","Fish","Chordata","Actinopterygii","Well water","22.6 - 24.8","Chronic","6.9 - 7.2","N.A.","Smith EJ, Sykora JL and Shapiro MA (1973). Effect of Lime Neutralized Iron Hydroxide Suspensions on Survival, Growth, and Reproduction of the Fathead Minnow (Pimephales promelas). Journal of the Fisheries Research Board of Canada, 30(8), 1147-1153. \n","220","Hardness  = 139 - 238 mg/L gradient . DID NOT PASS QAQC Q7"]
["Freshwater/metal/non- plant","221-1",54,0.54,"","Salvelinus fontinalis","3 months old","19 months","NR","Mortality","7800 - 13400 ug/L (total)","Fish","Chordata","Actinopterygii","Well water","10.8 - 11.5 oC","Chronic","7 - 7.3","N.A.","Sykora JL, Smith EJ, Synak M and Shapiro MA (1975). Some observations on spawning of brook trout (Salvelinus Fontinalis, mitchill) in lime neutralized iron hydroxide suspensions. Water Research, 9(4), 451-458. \n","221","Hardness  = 128 - 228 mg/L. DID NOT PASS QAQC Q7"]
["Freshwater/metal/non- plant","221-2",54,0.54,"","Salvelinus fontinalis","Yearling","5 months","NR","Growth (length)","7500 - 12500 ug/L (total)","Fish","Chordata","Actinopterygii","Well water","10.8 - 11.5 oC","Chronic","7 - 7.3","N.A.","Sykora JL, Smith EJ, Synak M and Shapiro MA (1975). Some observations on spawning of brook trout (Salvelinus Fontinalis, mitchill) in lime neutralized iron hydroxide suspensions. Water Research, 9(4), 451-458. \n","221","Hardness  = 128 - 228 mg/L. DID NOT PASS QAQC Q7"]
["Freshwater/metal/non- plant","222-1",54,0.54,"","Oncorhynchus kisutch","30-d post hatch","30 days","NR","Mortality","> 3000 ug/L (total)","Fish","Chordata","Actinopterygii","Well water","10.9 - 11.6 oC","Chronic","7.76 - 8.12","N.A.","Smith EJ and Sykora JL (1976). Early developmental effects of lime-neutralized iron hydroxide suspensions on Brook Trout and Coho Salmon. Transactions of the American Fisheries Society, 105(2), 308-312. ","222","Hardenss = 159 - 180 mg/L. Gradient due to neutralisation with CaSO4. DID NOT PASS QAQC Q7"]

{"sheet":"Instruction","index":3,"n_rows":21,"n_cols":10,"rows_kept":21,"cut":false,"hidden":false,"merged":[[27,6,27,16],[14,6,14,7],[5,4,5,7]]}
[]
[]
[null,"Click here to see \"All Result\" -->","All Result"]
[]
[]
[null,null,null,null,"Freshwater "]
[]
[null,"Tabs contain questionaries -->           ","Freshwater/metal/nonplant",null,"Freshwater/non-metal/non-plant ",null,null,"Freshwater/metal/plant",null,"Freshwater/non-metal/plant"]
[]
[]
[]
[null,"Tabs contain score of paper and short answers to questions -->","Freshwater/metal/nonplant Worksheet",null,"Freshwater/non-metal/non-plant Worksheet",null,null,"Freshwater/metal/plant Worksheet",null,"Freshwater/non-metal/plant/ Worksheet"]
[]
[]
[null,null,"Marine ",null,null,null,"Terrestrial",null,null,"Sediment "]
[]
[null,"Tab contain questionaries -->","Marine or estuarine/non-plant",null,"Marine or estuarine/Plant",null,null,"Terrestrial Data QA",null,"Sediment Data QA"]
[]
[]
[]
[null,"Tab Contains all scores of paper --> ","Marine or estuarine/non-Plant Worksheet",null,"Marine or estuarine/Plant_Worksheet",null,null,"Terrestrial Quality",null,"Sediment Quality"]

{"sheet":"Sheet1","index":4,"n_rows":70,"n_cols":29,"rows_kept":70,"cut":false,"hidden":true,"merged":[]}
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[null,null,null,{"$f":"IF(AU3=\"\",0,1)","v":1},null,{"$f":"IF(T3=\"\",0,1)","v":1},"Toxicity endpoint",null,null,"Acute Or Chronic ",null,null,null,null,"Asplanchna brightwellii",null,null,"1-11 d old",null,null,"13 days",null,null,"EC20",null,null,"esterase activity",null,null,"Insect",null,null,"Chlorophyta",null,null,"Branchiopoda",null,null,"Not Reported",null,null,"13°C",null,null,null,null,null,6.1]
[null,null,null,null,null,{"$f":"IF(W3=\"\",0,1)","v":1},"Toxicity value","> 3000 ug/L (total)",null,"PH",null,null,null,null,"Australian Bass",null,null,"22 mm",null,null,"14 days",null,null,"EC50",null,null,"fertilisation",null,null,"macroinvertebrate",null,null,"Chordata",null,null,"Branchiopoda",null,null,"Reconstituted softwater",null,null,"14-15°C",null,null,null,null,null,6.2]
[null,2,"Select the appropriate answer option to each question. Comments can be added to the cells in column H.",null,null,{"$f":"IF(Z3=\"\",0,1)","v":1},null,null,null,"Salinity/Conductivity","N.A.",null,null,null,"Austro-potamobius pallipes pallipes",null,null,"3 months old",null,null,"14 weeks",null,null,"EC84",null,null,"Growth (length)",null,null,"Mayfly",null,null,"Ciliophora",null,null,"Chlorophyceae",null,null,"Well water",null,null,"15°C",null,null,null,null,null,6.3]
[null,"*","If the selected answer option does not work try clicking on any other cell first and then click back on the answer option again.",null,null,{"$f":"IF(AC3=\"\",0,1)","v":1},null,null,null,null,null,null,null,null,"Brachionus calyciflorus",null,null,"30-d post hatch",null,null,"16 days",null,null,"ED50",null,null,"Growth (relative generation time)",null,null,"Microalga",null,null,"Insecta",null,null,"Clitellata",null,null,null,null,null,"16°C",null,null,null,null,null,6.5]
[null,"No.","Question","Marks Awarded","Answer Options","Option References","Scores","Answers",null,null,null,null,null,null,"Brachionus clacyflorus",null,null,"4 months old",null,null,"19 months",null,null,"ED50 (EC50)",null,null,"Growth (weight)",null,null,"Microinvertebrate",null,null,"Mollusca",null,null,"Clitellata",null,null,null,null,null,"18°C",null,null,null,null,null,6.6]
[null,null,"Was the duration of the exposure stated (e.g. 48 or 96 h)?",10,null,1,{"$f":"IF(F15=0,0,INDEX($D12:$D13,F12,1))","v":10},"30 d post-hatch",null,null,null,null,null,null,"Brachydanio rerio",null,null,"6-10 mm males",null,null,"21 d",null,null,"IC50",null,null,"hatching success",null,null,"Mite",null,null,"N.A.",null,null,"Eurotatoria",null,null,null,null,null,"20°C",null,null,null,null,null,6.8]
[null,1,null,0,null,null,null,null,null,null,null,null,null,null,"Branchiura sowerbyi",null,null,"6-12h post fertilisation",null,null,"24 d",null,null,"LC10",null,null,"hatching time",null,null,"Mussel",null,null,"Nematoda",null,null,"Gastropoda",null,null,null,null,null,"21°C",null,null,null,null,null,7]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Bursaphelenchus xylophilus",null,null,"69.9 mm",null,null,"24 h",null,null,"LC5",null,null,"heart rate",null,null,"N.A.",null,null,"Rotifer",null,null,"Insecta",null,null,null,null,null,"22 °C",null,null,null,null,null,7.2]
[null,null,"Was the biological endpoint (e.g. immobilization or population growth) stated and defined (10 marks)? Award 5 marks if the biological endpoint is only stated.",10,null,3,{"$f":"IF(F15=0,0,INDEX($D15:$D17,F15,1))","v":0},null,null,null,null,null,null,null,"Caridina nilotica",null,null,"Adult",null,null,"30 d",null,null,"LC50",null,null,"Immobility",null,null,"Oligochaete",null,null,"Tracheophyta",null,null,"Malocostraca",null,null,null,null,null,"22.6 - 24.8",null,null,null,null,null,7.3]
[null,2,null,5,null,null,null,null,null,null,null,null,null,null,"Ceriodaphnia dubia",null,null,"Adult male",null,null,"30 d post hatch",null,null,"LC84",null,null,"luminescence inhibition",null,null,"Ostracod",null,null,null,null,null,"Maxillopoda",null,null,null,null,null,"22-28",null,null,null,null,null,7.4]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Cherax destructor",null,null,"embryo",null,null,"30 days",null,null,"LOEC",null,null,"Mortality",null,null,"Protozoan",null,null,null,null,null,"N.A.",null,null,null,null,null,"23°C",null,null,null,null,null,7.5]
[null,null,"Was the biological effect stated (e.g. LC or NOEC)?",5,null,2,{"$f":"IF(F18=0,0,INDEX($D18:$D19,F18,1))","v":0},null,null,null,null,null,null,null,"Cheumatopsyche",null,null,"exponentially growing",null,null,"30 min",null,null,"LT50",null,null,"N.A.",null,null,"Rotifer",null,null,null,null,null,"Oligohymenophorea",null,null,null,null,null,"24°C",null,null,null,null,null,7.6]
[null,3,null,0,null,null,null,null,null,null,null,null,null,null,"Chironomus javanus",null,null,"fingerlings",null,null,"32 h",null,null,"N.A.",null,null,"not reported",null,null,"Snail",null,null,null,null,null,"Ostracoda",null,null,null,null,null,"24-26",null,null,null,null,null,7.7]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Chironomus riparius",null,null,"First Instar stage",null,null,"33 d",null,null,"NOAEC",null,null,"Reproduction",null,null,null,null,null,null,null,null,"Secernentea",null,null,null,null,null,"25°C",null,null,null,null,null,7.8]
[null,4,"Was the biological effect quantified (e.g. 50% effect, 25% effect)? \nNote: The effect for NOEC and LOEC data must be quantified.",5,null,2,{"$f":"IF(F21=0,0,INDEX($D21:$D22,F21,1))","v":0},null,null,null,null,null,null,null,"Cyclops viridis",null,null,"Fourth Instar Larvae",null,null,"35 weeks",null,null,"NOEC",null,null,"reproduction",null,null,null,null,null,null,null,null,null,null,null,null,null,null,"26°C",null,null,null,null,null,7.8]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Cyprinus carpio",null,null,"Fry",null,null,"40 min",null,null,"NR",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"28 - 30 oC",null,null,null,null,null,8]
[null,5,"Were appropriate controls (e.g. a no-toxicant control and/or solvent control) used?",5,null,1,{"$f":"IF(F23=0,0,INDEX($D23:$D24,F23,1))","v":5},null,null,null,null,null,null,null,"Cypris subglobosa",null,null,"gamete",null,null,"48 h",null,null,"Threshold conc",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"28°C",null,null,null,null,null,8.1999999999999993]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Daphnia longispina",null,null,"glochidia",null,null,"5 d",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"30°C",null,null,null,null,null,8.4]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Daphnia magna",null,null,"Instar",null,null,"5 months",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"6 oC",null,null,null,null,null,8.5]
[null,6,"Was each control and contaminant concentration at least duplicated?",5,null,1,{"$f":"IF(F26=0,0,INDEX($D26:$D27,F26,1))","v":5},"2 replicates",null,null,null,null,null,null,"Daphnia pulex",null,null,"juvenile",null,null,"50 h",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"8 - 21.6 oC",null,null,null,null,null,9]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Diaptomus forbesi",null,null,"larvae",null,null,"52 h",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"N.A.",null,null,null,null,null,84]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Duttaphrynus melanostictus",null,null,"mixed age",null,null,"55 h",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"N.R,.",null,null,null,null,null,"2015-07-08"]
[null,7,"Were test acceptability criteria stated (e.g. mortality in controls must not exceed a certain percentage) (5 marks)?\nOR Were test acceptability criteria inferred (e.g. test method used [USEPA, OECD, ASTM etc.]) (award 2 marks). \nNote: Data that fail the acceptability criteria are automatically deemed to be of unacceptable quality and must not be used. ",5,null,3,{"$f":"IF(F29=0,0,INDEX($D29:$D31,F29,1))","v":0},null,null,null,null,null,null,null,"Ephemerella subvaria",null,null,"N.A.",null,null,"7 d",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"N.R.",null,null,null,null,null,">5"]
[null,null,null,2,null,null,null,null,null,null,null,null,null,null,"Gambusia affinis",null,null,"neonate",null,null,"72 h",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"10.9 - 11.6 oC",null,null,null,null,null,"3.5-7.42"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Gammarus minus",null,null,"Not reported",null,null,"9 d",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"4.4-4.6"]
[null,8,"Were the characteristics of the test organism (e.g. length, mass, age) stated?",5,null,1,{"$f":"IF(F32=0,0,INDEX($D32:$D34,F32,1))","v":5},"30 d post hatch",null,null,null,null,null,null,"Hyalella azteca",null,null,"nymph",null,null,"9 h",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"4.9 ± 2.0"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Hydropsyche betteni",null,null,"sperm",null,null,"96 h",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"5.5-9"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Lebistes reticulatus",null,null,"sperm and eggs together",null,null,"First Instar stage",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"5.8-6.0"]
[null,9,"Was the type of test media used stated?",5,null,1,{"$f":"IF(F35=0,0,INDEX($D35:$D37,F35,1))","v":5},"well water",null,null,null,null,null,null,"Lecane quadridentata",null,null,"tadpoles",null,null,"N.A.",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"5.9 ± 1.7"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Lepomis macrochirus",null,null,"Third Instar Larvae",null,null,"N.R.",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"5.92-8.41"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Leptophlebia marginata",null,null,"unfertilised egg",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"6.1 ± 1.8"]
[null,null,"Was the type of exposure (e.g. static, flow through) stated? ",4,null,1,{"$f":"IF(F38=0,0,INDEX($D38:$D40,F38,1))","v":4},"flow-through",null,null,null,null,null,null,"Lirceus fontinalis",null,null,"Yearling",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"6.1-6.7"]
[null,10,null,0,null,null,null,null,null,null,null,null,null,null,"Margaritifera margaritifera",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"6.3 ± 1.1"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Melanoides tuberculata",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"6.4 ± 1.4"]
[null,null,"Were the contaminant concentrations measured at the beginning and end of the exposure (4 marks)? Award 2 marks if they were measured only once during the test.\nNote: If the concentrations were not measured then automatically the data cannot be high quality. ",4,null,1,{"$f":"IF(F41=0,0,INDEX($D41:$D43,F41,1))","v":4},"iron speciation (dissolved, total, Fe2+)",null,null,null,null,null,null,"N.A.",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"6.5 - 7.9"]
[null,11,null,2,null,null,null,null,null,null,null,null,null,null,"Nais elinguis",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"6.7 ± 0.1"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Oncorhynchus mykiss",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"6.86 - 7.16"]
[null,null,"Were parallel reference toxicant toxicity tests conducted?",4,null,2,{"$f":"IF(F44=0,0,INDEX($D44:$D46,F44,1))","v":0},null,null,null,null,null,null,null,"Orconectes limosus",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"6.9 - 7.2"]
[null,12,null,0,null,null,null,null,null,null,null,null,null,null,"Oryzias latipes",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"6.9-7.5"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Philodina acuticornis",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"7 - 7.3"]
[null,null,"Was there a concentration–response relationship either observable or stated?",4,null,1,{"$f":"IF(F47=0,0,INDEX($D47:$D49,F47,1))","v":4},null,null,null,null,null,null,null,"Physa gyrina",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"7 ± 1"]
[null,13,null,0,null,null,null,null,null,null,null,null,null,null,"Pimephales promelas",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"7.0 - 7.3"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Planorbarius",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"7.0-7.3"]
[null,null,"Was an appropriate statistical method or model used to determine the toxicity?\nNote: They should be accepted by a recognised national or international regulatory body (e.g. USEPA, OECD, ASTM). ",4,null,2,{"$f":"IF(F50=0,0,INDEX($D50:$D52,F50,1))","v":0},"no statistics used",null,null,null,null,null,null,"Plsnibarius sp",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"7.1 - 7.6"]
[null,14,null,0,null,null,null,null,null,null,null,null,null,null,"Poecilia reticulata",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"7.25 - 7.7"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Rana hexadactyla",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"7.25 - 8.1"]
[null,15,"For LC/EC/BEC data was an estimate of variability provided? \nOR \nFor NO EC/LOEC data was the significance level 0.05 or less?  \n\n",4,null,2,{"$f":"IF(F53=0,0,INDEX($D53:$D55,F53,1))","v":0},null,null,null,null,null,null,null,"Rasbora sumatrana ",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"7.25 - 8.2"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Salmo gairdneri",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"7.2-7.6"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Salmo trutta",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"7.6 ± 0.2"]
[null,16,"Were the following parameters measured and stated? (3 marks if measured and stated, 1 mark if just measured).",null,null,null,null,null,null,null,null,null,null,null,"Salvelinus fontinalis",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"7.6 ± 0.2"]
[null,null,null,3,null,1,{"$f":"IF(F57=0,0,INDEX($D57:$D59,F57,1))","v":3},"7.76 - 8.12",null,null,null,null,null,null,"Selvelinus fontinalis",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"7-8"]
[null,16.100000000000001,"pH",1,null,null,null,null,null,null,null,null,null,null,"Stenocypris major",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"8.0-8.2"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Streptocephalus proboscideus",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"N.A."]
[null,null,null,3,null,1,{"$f":"IF(F60=0,0,INDEX($D60:$D62,F60,1))","v":3},"159 - 180 mg/L gradient as a result of neutralizing Fe with Ca(OH)2",null,null,null,null,null,null,"Tetrahymena pyriformis",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Not Reported"]
[null,16.2,"Hardness",1,null,null,null,null,null,null,null,null,null,null,"Tetrahymena pyriformis",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"PH11"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Tetrahymena thermophila",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"PH7.0"]
[null,null,null,3,null,3,{"$f":"IF(F63=0,0,INDEX($D63:$D65,F63,1))","v":0},null,null,null,null,null,null,null,"Tilapia mossambica",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"reported to be 'low'"]
[null,16.3,"Alkalinity",1,null,null,null,null,null,null,null,null,null,null,"Tubifex tubifex",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"7.76 - 8.12"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Vibrio fisheri"]
[null,null,null,3,null,3,{"$f":"IF(F66=0,0,INDEX($D66:$D68,F66,1))","v":0},null,null,null,null,null,null,null,"Oncorhynchus kisutch"]
[null,16.399999999999999,"Organic carbon concentration",1]
[null,null,null,0]
[null,null,null,3,null,1,{"$f":"IF(F69=0,0,INDEX($D69:$D71,F69,1))","v":3},"93.6 - 100 % saturation"]
[null,16.5,"Dissolved Oxygen",1]
[null,null,null,0]
[null,null,"Was the temperature measured and stated (3 marks)? Award 1 mark if the temperature was measured but not stated or if only the temperature settings of the room or chamber are stated.",3,null,1,{"$f":"IF(F72=0,0,INDEX($D72:$D74,F72,1))","v":3},"10.9 - 11.6 oC"]
[null,17,null,1]
[null,null,null,0]
[null,null,"Were test solutions, blanks and/or controls tested for contamination OR were analytical reagent grade chemicals used or the highest possible purity chemicals used for the experiment? (3 marks).",3,null,2,{"$f":"IF(F75=0,0,INDEX($D75:$D76,F75,1))","v":0}]
[null,18,null,0]
[]
[null,null,null,null,null,null,null,null,{"$f":"(COUNTIF(F12:F76,\"=0\"))+COUNTIF(F2:F10,\"=0\")+COUNTIF(D2:D7,\"=0\")","v":0}]
[]
[]
[null,null,"Total score",null,null,null,{"$f":"IF(SUM(G12:G75)=0,\"\",SUM(G12:G75))","v":54}]
[null,null,"Total Max. possible score for the various types of data and contaminants ",null,{"$f":"D12+D15+D18+D21+D23+D26+D29+D32+D35+D38+D41+D44+D47+D50+D53+D57+D66+D69+D72+D75+D63+D60","v":100}]
[null,null,"Quality Score",null,{"$f":"\"= [Total score / Total possible score] * 100 = \"","v":"= [Total score / Total possible score] * 100 = "},null,{"$f":"IF(ISERROR(G81/E82),#N/A,G81/E82)","v":0.54},{"$f":"IF(F41=3,\"The highest possible grade for this datum is acceptable.\",\"\")","v":""}]
[]
[null,null,"H = high quality                           Quality score ≥ 80%     "]
[null,null,"A = acceptable quality         > 50% Quality score < 80%  "]
[null,null,"U = unacceptable quality              Quality score ≤ 50% "]
[]
[3,null,"Click the 'Save & Update' button to save and export the answers to the 'All results' worksheet. "]
[null,null,"Select the 'Reset Answers' button if you wish to clear all answers."]

{"sheet":"FW metal nonplant_WS","index":6,"n_rows":779,"n_cols":26,"rows_kept":779,"cut":false,"hidden":false,"merged":[]}
[]
["Question No.",1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16.100000000000001,16.2,16.3,16.399999999999999,16.600000000000001,17,18,"Total Score","Quality Score"]
["Short Questions","Duration of the exposure ","Biological endpoint","Biological effect stated or not","Biological effect quantified or not","Appropriate controls","Control & contaminant concentration","Test acceptability criteria","Characteristics of the test organism"," Type of test media","Type of exposure","Contaminant concentrations","Parallel reference toxicant toxicity tests","Concentration–response relationship","Determine the toxicity","NOEC/LOEC or LC/EC/BEC","pH","Hardness","Alkalinity","Organic carbon concentration","Dissolved Oxygen","Temperature","Grade or purity"]
["Marks Awarded ","10 or 0","10,5 or 0","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","4 or 0","4 or 2 or 0","4 or 2","4 or 2","4 or 2","4 or 2","3 or 1 or 0","3 or 1 or 0","3 or 1 or 0","3 or 1 or 0","3 or 1 or 0","3 or 0","3 or 0 "]
["Max. Possible Score",10,10,5,5,5,5,5,5,5,4,4,4,4,4,4,3,3,3,3,3,3,3,{"$f":"SUM(B5:W5)","v":100}]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","151-1"]
["Scores",10,5,5,5,5,0,0,5,0,4,0,0,4,4,0,0,0,0,0,0,1,0,48,0.48,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","Mortality","LC",50,"non-toxicant controls","10 fish per aquaria","","3.2 cm","","Daily or bi-daily renewal","","","Observed in Figures","Probit and Trimmed Spearman Karber","","","","","","","15 °C",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","151-2"]
["Scores",10,5,5,5,5,0,0,5,0,4,0,0,4,4,0,0,0,0,0,0,1,0,48,0.48,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","Mortality","LC",50,"non-toxicant controls","10 fish per aquaria","","3.2 cm","","Daily or bi-daily renewal","","","Observed in Figures","Probit and Trimmed Spearman Karber","","","","","","","15 °C",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","151-3"]
["Scores",10,5,5,5,5,0,0,5,0,4,0,0,4,4,0,0,0,0,0,0,1,0,48,0.48,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","Mortality","LC",50,"non-toxicant controls","10 fish per aquaria","","6.0 cm","","Daily or bi-daily renewal","","","Observed in Figures","Probit and Trimmed Spearman Karber","","","","","","","15 °C",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","151-4"]
["Scores",10,5,5,5,5,0,0,5,0,4,0,0,4,4,0,0,0,0,0,0,1,0,48,0.48,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","Mortality","LC",50,"non-toxicant controls","10 fish per aquaria","","6.0 cm","","Daily or bi-daily renewal","","","Observed in Figures","Probit and Trimmed Spearman Karber","","","","","","","15 °C",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","152-1"]
["Scores",10,10,5,5,5,0,0,5,5,4,0,0,0,4,0,0,0,0,0,0,1,0,54,0.54,"The highest possible grade for this datum is acceptable."]
["Answers","32 h","Immobility determined by placing the rack of bottles containing animals in front of a lighted translucent screen.","Threshold concentration (akin to NOEC)","Highest concentration that does not cause immobilisation.","Lake water Controls","","","<8h old","Lake water","Static","","","","Graphical estimation","","","","","","","25°C",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","152-2"]
["Scores",10,10,5,5,5,0,0,5,5,4,0,0,0,4,0,0,0,0,0,0,1,0,54,0.54,"The highest possible grade for this datum is acceptable."]
["Answers","32 h","Immobility determined by placing the rack of bottles containing animals in front of a lighted translucent screen.","Threshold concentration (akin to NOEC)","Highest concentration that does not cause immobilisation.","Lake water Controls","","","<8h old","Lake water","Static","","","","Graphical estimation","","","","","","","25°C","FeSO4 salt used. Value for toxicity converted from ppm FeSO4 to µg/L Fe"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","155-1"]
["Scores",10,5,5,5,5,5,0,5,5,4,0,0,0,4,0,3,0,0,0,0,1,3,60,0.6,"The highest possible grade for this datum is acceptable."]
["Answers","1 h","FDA-esterase test. Measured by spectrofluorimetric reader.","IC",50,"non-toxicant controls","Experiments were repeated 6-8 times. ","","Exponentially growing cells","Volvic mineral water","Static","","","Table of IC50 values for 34 chemcials, but no mention of dose-response relationship","Regression analyses after logarithmic transformation","","5.5-9.0","","","","","28°C",">90% purity chemicals, FeCl3.6H2O used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","155-2"]
["Scores",10,10,5,5,5,5,0,5,5,4,0,0,0,4,0,0,0,0,0,0,1,3,62,0.62,"The highest possible grade for this datum is acceptable."]
["Answers","9 h","Generation time. i.e. time required for the Dx/D0 ratio (Dx = final cell density,D0 = initial) to increase from 1 to 2. Relative generation time : RGT = ((GTT-GTC)/GTCx100)","IC",50,"non-toxicant controls","triplicate","","Exponentially growing cells","Proteose-peptone yeast salts-defined medium (Plesner et al, 1964)","Static","","","Table of IC50 values for 34 chemcials, but no mention of dose-response relationship","Regression analyses after logarithmic transformation","","","","","","","28°C",">90% purity chemicals,  FeCl3.6H2O used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","155-3"]
["Scores",10,10,5,5,5,5,0,5,5,4,0,0,0,4,0,0,0,0,0,0,1,3,62,0.62,"The highest possible grade for this datum is acceptable."]
["Answers","9 h","Generation time. i.e. time required for the Dx/D0 ratio (Dx = final cell density,D0 = initial) to increase from 1 to 2. Relative generation time : RGT = ((GTT-GTC)/GTCx100)","IC",50,"non-toxicant controls","triplicate","","Exponentially growing cells","Proteose-peptone yeast salts-defined medium (Plesner et al, 1964)","Static","","","Table of IC50 values for 34 chemcials, but no mention of dose-response relationship","Regression analyses after logarithmic transformation","","","","","","","28°C",">90% purity chemicals, FeSO4.7H2O used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","155-4"]
["Scores",10,5,5,5,5,5,0,5,5,4,0,0,0,4,0,0,0,0,0,0,1,3,57,0.56999999999999995,"The highest possible grade for this datum is acceptable."]
["Answers","1 h","Esterase activity measured by spectrophotometric methods","IC",50,"non-toxicant controls","repeated 6-8 times","","Exponentially growing cells","Volvic mineral water","Static","","","Table of IC50 values for 34 chemcials, but no mention of dose-response relationship","Regression analyses after logarithmic transformation","","","","","","","28°C",">90% purity chemicals, FeSO4.7H2O used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","155-5"]
["Scores",10,5,5,5,5,5,0,0,0,4,0,0,0,4,0,0,0,0,0,0,0,3,46,0.46,"The highest possible grade for this datum is acceptable."]
["Answers","30 min","referred to standard Microtox methods","EC",50,"non-toxicant controls","triplicate","","","","Static","","","Table of IC50 values for 34 chemcials, but no mention of dose-response relationship","Microtox statistics program","","","","","","","",">90% purity chemicals, FeSO4.7H2O used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","155-6"]
["Scores",10,5,5,5,5,5,0,0,0,4,0,0,0,4,0,0,0,0,0,0,0,3,46,0.46,"The highest possible grade for this datum is acceptable."]
["Answers","30 min","referred to standard Microtox methods","EC",50,"non-toxicant controls","triplicate","","","","Static","","","Table of IC50 values for 34 chemcials, but no mention of dose-response relationship","Microtox statistics program","","","","","","","",">90% purity chemicals, FeCl3.6H2O used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","156-1"]
["Scores",10,10,5,5,5,5,2,5,5,4,0,0,0,4,4,1,0,0,0,0,1,3,69,0.69,"The highest possible grade for this datum is acceptable."]
["Answers","1 h","Enzyme activity in cells, measured by FDA fluorescence, spectrophotometric method.","EC",50,"non toxicant controls","Experiments repeated 6-8 times","No growth in Controls.","Exponentially growing cells","Volvic mineral water","Static","","","not stated.","Regression analyses, StatView SE 1.03. Concentrations were transformed into logarithms to fit regression model.","CV of 46%, SD of 1.1 mg/L","25°C","","","","","25°C",">98% purity chemicals, FeCl3.6H2O used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","157-1"]
["Scores",10,5,5,5,5,5,5,5,5,4,2,0,4,4,4,3,3,3,3,3,3,3,89,0.89,""]
["Answers","7 d","Survival/mortality","LC",50,"non-toxicant controls and acid controls","Minimum of two repeat results per test treatment. Modified test design, where 1-3 concentrations of metal was run in each test (since so many metals tested). Data were then pooled and analyzed as if they had all been obtained from a single test.","Only test data with >/= 80% survival in Controls used.","1-11 d old","Synthetic soft water","Static, non-renewal","Highest test conc of 1000µg/L nominal = 15 µg/L measured dissolved Fe in softwater.","","Description of method involves observing the response of the animals to toxicant and ensuring 0 (Control) and 25%-75% effects.","Trimmed Spearman Karber","CVs provided, but for Fe, toxicity was>highest test concentration","Controls 7.4 (mean ) 6.8-7.8 (range); test concentrations 7.4 (mean), 6.4-8.5 (range)","18 mg CaCO3/L","14 mg/L CaCO3","0.28 mg/L","7-10 mg/L","24.7 (mean) 24-25 (range)","Controls were analysed for contamination, AAS standards used for Fe."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","157-2"]
["Scores",10,5,5,5,5,5,5,5,5,4,0,0,4,4,4,3,3,3,3,3,3,3,87,0.87,"The highest possible grade for this datum is acceptable."]
["Answers","7 d","Survival/mortality","LC",50,"non-toxicant controls and acid controls","Minimum of two repeat results per test treatment. Modified test design, where 1-3 concentrations of metal was run in each test (since so many metals tested). Data were then pooled and analyzed as if they had all been obtained from a single test.","Only test data with >/= 80% survival in Controls used.","1-11 d old","Tap water","Static, non-renewal","Other metals measured, but due to low toxicity Fe in tap water not measured (or at least not reported).","","Description of method involves observing the response of the animals to toxicant and ensuring 0 (Control) and 25%-75% effects.","Trimmed Spearman Karber","CVs provided, but for Fe, toxicity was>highest test concentration","Controls 8.4 (mean ) 8.1-8.8 (range); test concentrations 8.2 (mean), 7.2-8.3 (range)","124 mg CaCO3/L","84 mg/L CaCO3","1.1 mg/L","7-10 mg/L","24.7 (mean) 24-25 (range)","Controls were analysed for contamination, AAS standards used for Fe."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","158-1"]
["Scores",10,10,5,5,5,0,2,5,0,4,2,0,4,0,0,3,0,0,0,1,1,0,57,0.56999999999999995,""]
["Answers","96 h","Immobilisation - full immobilisation of body parts, but often heart still beating","LC",50,"Assumed, as it followed APHA method","Not stated (although French aticle, so may be missed)","APHA 1960 method","Individuals with rostrum length beween 19-32 mm at the stage of exoskeletal stability","","Static","Measured by AAS","","In graphs","Unclear, although probably followed APHA methods.","","7 ± 0.3","","","","stated to be at saturation","16 ± 1°C",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","158-2"]
["Scores",10,10,5,5,5,0,2,5,0,4,2,0,4,0,0,3,0,0,0,1,1,0,57,0.56999999999999995,""]
["Answers","30 d","Immobilisation - full immobilisation of body parts, but often heart still beating. ","EC (note no food added)",50,"Assumed, as it followed APHA method","Not stated (although French aticle, so may be missed)","APHA 1960 method","Individuals with rostrum length beween 19-32 mm at the stage of exoskeletal stability","","Static","Measured by AAS","","In graphs","Unclear, although probably followed APHA methods.","","7 ± 0.3","","","","stated to be at saturation","16 ± 1°C","FeCL3.6H2O used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","158-3"]
["Scores",10,10,5,5,5,0,2,5,0,4,2,0,4,0,0,3,0,0,0,1,1,0,57,0.56999999999999995,""]
["Answers","30 d","Immobilisation - full immobilisation of body parts, but often heart still beating. ","EC (note food added)",50,"Assumed, as it followed APHA method","Not stated (although French aticle, so may be missed)","APHA 1960 method","Individuals with rostrum length beween 19-32 mm at the stage of exoskeletal stability","","Static","Measured by AAS","","In graphs","Unclear, although probably followed APHA methods.","","7 ± 0.3","","","","stated to be at saturation","16 ± 1°C","FeCL3.6H2O used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","158-4"]
["Scores",10,10,5,5,5,0,2,5,0,4,2,0,4,0,0,3,0,0,0,1,1,0,57,0.56999999999999995,""]
["Answers","30 d","Immobilisation - full immobilisation of body parts, but often heart still beating. ","EC (note food added)",50,"Assumed, as it followed APHA method","Not stated (although French aticle, so may be missed)","APHA 1960 method","Individuals with rostrum length beween 19-32 mm at the stage of exoskeletal stability","","Static","Measured by AAS","","In graphs","Unclear, although probably followed APHA methods.","","7 ± 0.3","","","","stated to be at saturation","16 ± 1°C","FeCL3.6H2O used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","158-5"]
["Scores",10,10,5,5,5,0,2,5,0,4,2,0,4,0,0,3,0,0,0,1,1,0,57,0.56999999999999995,""]
["Answers","30 d","Immobilisation - full immobilisation of body parts, but often heart still beating. ","EC (note, NO food added)",50,"Assumed, as it followed APHA method","Not stated (although French aticle, so may be missed)","APHA 1960 method","Individuals with rostrum length beween 19-32 mm at the stage of exoskeletal stability","","Static","Measured by AAS","","In graphs","Unclear, although probably followed APHA methods.","","7 ± 0.3","","","","stated to be at saturation","16 ± 1°C","FeCL3.6H2O used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","158-6"]
["Scores",10,10,5,5,5,0,2,5,0,4,2,0,4,0,0,3,0,0,0,1,1,0,57,0.56999999999999995,""]
["Answers","96 h","Immobilisation - full immobilisation of body parts, but often heart still beating. ","EC ",50,"Assumed, as it followed APHA method","Not stated (although French aticle, so may be missed)","APHA 1960 method","Individuals with rostrum length beween 19-32 mm at the stage of exoskeletal stability","","Static","Measured by AAS","","In graphs","Unclear, although probably followed APHA methods.","","7 ± 0.3","","","","stated to be at saturation","16 ± 1°C","FeCL3.6H2O used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","159-1"]
["Scores",10,10,5,5,5,5,2,0,5,4,2,0,0,4,0,1,1,1,0,0,1,3,64,0.64,""]
["Answers","24 h","The criterion for \"affected\" was no visible internal or external motion of an animal when exposed to bright light.","EC",50,"Non-toxicant controls (seen on graphs)","triplicate","[American Public Health Association, 1973].","","Synthetic softwater","Static","Measured by AAS.","","EC50 values in Table","EC-50 values were determined by the straight line estimation method [American Public Health Association, 1973].","",7.5,"40 mg/L CaCO3","35 mg/L CaCO3","","","20 ± 0.3°C","FeSO4.7H2O used. Controls may have been measured, but it is not stated. ACS grade chemicals used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","159-2"]
["Scores",10,10,5,5,5,5,2,0,5,4,2,0,0,4,0,1,1,1,0,0,1,3,64,0.64,""]
["Answers","24 h","The criterion for \"affected\" was no visible internal or external motion of an animal when exposed to bright light.","EC",50,"Non-toxicant controls (seen on graphs)","triplicate","[American Public Health Association, 1973].","","Synthetic softwater","Static","Measured by AAS.","","EC50 values in Table","EC-50 values were determined by the straight line estimation method [American Public Health Association, 1973].","",7.5,"40 mg/L CaCO3","35 mg/L CaCO3","","","20 ± 0.3°C","FeSO4.7H2O used. Controls may have been measured, but it is not stated. ACS grade chemicals used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","159-3"]
["Scores",10,10,5,5,5,5,2,0,5,4,2,0,0,4,0,1,1,1,0,0,1,3,64,0.64,""]
["Answers","24 h","The criterion for \"affected\" was no visible internal or external motion of an animal when exposed to bright light.","EC",50,"Non-toxicant controls (seen on graphs)","triplicate","[American Public Health Association, 1973].","","Synthetic softwater","Static","Measured by AAS.","","EC50 values in Table","EC-50 values were determined by the straight line estimation method [American Public Health Association, 1973].","",7.5,"40 mg/L CaCO3","35 mg/L CaCO3","","","20 ± 0.3°C","FeSO4.7H2O used. Controls may have been measured, but it is not stated. ACS grade chemicals used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","160-1"]
["Scores",10,10,0,5,5,5,2,5,5,0,2,0,0,4,4,1,0,0,0,0,1,3,62,0.62,""]
["Answers","7 d","Mortality of embryos or larvae was determined by examining movement,\nheartbeat and blood circulation. ","But inferred from data","NOEC from Table of data and significnace testing. ","Media-only (ERM) control","96 well plates, 1 embryo per well. Experiment also repeated at least twice.","No mortalities in controls.","Embryo at late stage 10, early blastula","ERM, embryo rearing media, recipe given in paper.","Assumed to be static","Total Iron measured","","Slight concentration response effect seen, but full curve not tested, i.e. concentrations were not high enough to cause more than 28% effect. Also an interruption to the conc effect.","ANOVA, Duncans test, 0.05 significnace level.","0.05 significance level.",7.2,"Could be clauclated from Mg and Ca in recipe for media","","","","26 ± 1 °C","All test concs were measured for total iron, inlcuding Controls. 0 mg/L in Controls."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","160-2"]
["Scores",10,10,0,5,5,5,2,5,5,0,2,0,4,4,4,1,0,0,0,0,1,3,66,0.66,""]
["Answers","7 d","Heart rate in embryos 5 days post fertilisation","But inferred from data","LOEC from Figure 3 of data and significnace testing. ","Media-only (ERM) control","96 well plates, 1 embryo per well. Experiment also repeated at least twice.","No mortalities in controls.","Embryo at late stage 10, early blastula. 5 dpf","ERM, embryo rearing media, recipe given in paper.","Assumed to be static","Total Iron measured","","Stated in text and seen in Figure","ANOVA, Duncans test, 0.05 significnace level.","0.05 significance level.",7.2,"Could be clauclated from Mg and Ca in recipe for media","","","","26 ± 1 °C","All test concs were measured for total iron, inlcuding Controls. 0 mg/L in Controls."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","160-3"]
["Scores",10,10,0,5,5,5,2,5,5,0,2,0,4,4,4,1,0,0,0,0,1,3,66,0.66,""]
["Answers","7 d","Hatching success was determined as the breakdown and exiting of larval fish from the egg chorion.","But inferred from data","LOEC from Figure 3 of data and significnace testing. ","Media-only (ERM) control","96 well plates, 1 embryo per well. Experiment also repeated at least twice.","No mortalities in controls.","Embryo at late stage 10, early blastula. 5 dpf","ERM, embryo rearing media, recipe given in paper.","Assumed to be static","Total Iron measured","","Stated in text and seen in Figure","ANOVA, Duncans test, 0.05 significnace level.","0.05 significance level.",7.2,"Could be clauclated from Mg and Ca in recipe for media","","","","26 ± 1 °C","All test concs were measured for total iron, inlcuding Controls. 0 mg/L in Controls."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","161-1"]
["Scores",10,5,0,5,5,5,2,5,5,4,2,0,4,0,4,3,0,0,0,3,1,3,66,0.66,""]
["Answers","14 d","Mortality","But inferred from data","NOEC atainable from Graph and text","ERM controls","Four replciates and experiment carried out at least twice.",">90% survival in controls from Figure.","7 days old larvae","ERM, recipe given","daily renewal","Measurements done but not stated, other than for a 100 mg/L solution, where all was present as Fe(II).","","Described and on graph","Students T test was done. ","0.05 level of significance","pH plotted over time in Controls and 2 concentrations of Fe(II). pH in Controls = 6.5-7. pH in 25 mg/L = 5.5-7. pH in 100 mg/L was 5.3-7 (read from graph so not completely accurate).","","","","Plotted on graph. DO in Controls, 25 and 100 mg/L Fe(II) was around 7 mg/L","26 ± 2°C","AR Grade, Fe(II), Ferrous sulfate"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","162-1"]
["Scores",10,5,0,5,5,5,2,5,5,4,4,0,4,4,4,3,0,0,0,3,1,3,72,0.72,""]
["Answers","72-h","Mortality","But inferred from dta in graphs","NOEC readable from data in Figure 1.","ERM Controls.","4-12 replicates","0% mortality in graph","7-d old","ERM (embryo rearling media) recipe provided and reference","Daily renewal","Measured every day in each test solution before and after renewal as total Fe. Filterable (20 nm) Fe also determined for 100 mg/L solution on Day 0 and Day 1. >90% of Fe is Fe(II) filterable Fe.","","Seen on graphs","ANOVA followed by Dunacns test.","0.05 level of significance","In ERM Controls, pH around 6.5-7.5","","","","In ERM Controls, >7.5 mg/L.","26 ± 2°C","Note: Fe(II) measured in 100 mg/L solution. >90% was present as Fe(II) 'filterable' Fe (20nm). "]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","163-1"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The effect on the reproduction was followed by the number of neonates produced per adult individual, by counting the total number of neonates produced until either the death of the adult or the end of the experiment.","EC",50,"Non-toxicant Controls","10 replicates","Full Table of acceptability criteria","<24h old neonates","EPA synthetic water","Static, renewal daily.","Measured on Day 1, 4, 7. Total and dissolved. Value given for EC50 is total. Dissolved was<DL.","","Observed","USEPA database software, linear interpolation, probit, dunnets.","95% CLs","Across all treatments = 6.3-6.4","25 mg/L. ","17 mg/L",0,"across all treatments = 8.03-9.8 mg/L",25,"Controls measured for Fe total and dissolved concentrations. >80% purity Fe2(SO4)3.H2O used"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","163-2"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The effect on the reproduction was followed by the number of neonates produced per adult individual, by counting the total number of neonates produced until either the death of the adult or the end of the experiment.","EC",10,"Non-toxicant Controls","10 replicates","Full Table of acceptability criteria","<24h old neonates","EPA synthetic water","Static, renewal daily.","Measured on Day 1, 4, 7. Total and dissolved. Value given for EC50 is total. Dissolved was<DL.","","Observed","USEPA database software, linear interpolation, probit, dunnets.","95% CLs","Across all treatments = 6.3-6.4","25 mg/L. ","17 mg/L",0,"across all treatments = 8.03-9.8 mg/L",25,"Controls measured for Fe total and dissolved concentrations. >80% purity Fe2(SO4)3.H2O used"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","163-3"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The effect on the reproduction was followed by the number of neonates produced per adult individual, by counting the total number of neonates produced until either the death of the adult or the end of the experiment.","EC",10,"Non-toxicant Controls","10 replicates","Full Table of acceptability criteria","<24h old neonates","EPA synthetic water","Static, renewal daily.","Measured on Day 1, 4, 7. Total and dissolved. Value given for EC50 is total. Dissolved was<DL.","","Observed","USEPA database software, linear interpolation, probit, dunnets.","95% CLs","Across all treatments = 6.2-6.4","25 mg/L. ","17 mg/L",4,"across all treatments = 8.5-9.2 mg/L",25,"Controls measured for Fe total and dissolved concentrations. >80% purity Fe2(SO4)3.H2O used"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","163-4"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The effect on the reproduction was followed by the number of neonates produced per adult individual, by counting the total number of neonates produced until either the death of the adult or the end of the experiment.","EC",10,"Non-toxicant Controls","10 replicates","Full Table of acceptability criteria","<24h old neonates","EPA synthetic water","Static, renewal daily.","Measured on Day 1, 4, 7. Total and dissolved. Value given for EC50 is total. Dissolved was<DL.","","Observed","USEPA database software, linear interpolation, probit, dunnets.","95% CLs","Across all treatments = 6.2-6.4","25 mg/L. ","17 mg/L",4,"across all treatments = 8.5-9.2 mg/L",25,"Controls measured for Fe total and dissolved concentrations. >80% purity Fe2(SO4)3.H2O used"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","163-5"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The effect on the reproduction was followed by the number of neonates produced per adult individual, by counting the total number of neonates produced until either the death of the adult or the end of the experiment.","EC",50,"Non-toxicant Controls","10 replicates","Full Table of acceptability criteria","<24h old neonates","EPA synthetic water","Static, renewal daily.","Measured on Day 1, 4, 7. Total and dissolved. Value given for EC50 is total. Dissolved was<DL.","","Observed","USEPA database software, linear interpolation, probit, dunnets.","95% CLs","Across all treatments = 6.2-6.4","25 mg/L. ","17 mg/L",4,"across all treatments = 8.5-9.2 mg/L",25,"Controls measured for Fe total and dissolved concentrations. >80% purity Fe2(SO4)3.H2O used"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","163-6"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The effect on the reproduction was followed by the number of neonates produced per adult individual, by counting the total number of neonates produced until either the death of the adult or the end of the experiment.","EC",50,"Non-toxicant Controls","10 replicates","Full Table of acceptability criteria","<24h old neonates","EPA synthetic water","Static, renewal daily.","Measured on Day 1, 4, 7. Total and dissolved. Value given for EC50 is total. Dissolved was<DL.","","Observed","USEPA database software, linear interpolation, probit, dunnets.","95% CLs","Across all treatments = 6.2-6.4","25 mg/L. ","17 mg/L",4,"across all treatments = 8.5-9.2 mg/L",25,"Controls measured for Fe total and dissolved concentrations. >80% purity Fe2(SO4)3.H2O used"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","163-7"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The effect on the reproduction was followed by the number of neonates produced per adult individual, by counting the total number of neonates produced until either the death of the adult or the end of the experiment.","EC",50,"Non-toxicant Controls","10 replicates","Full Table of acceptability criteria","<24h old neonates","EPA synthetic water","Static, renewal daily.","Measured on Day 1, 4, 7. Total and dissolved. Value given for EC50 is total. Dissolved was<DL.","","Observed","USEPA database software, linear interpolation, probit, dunnets.","95% CLs","Across all treatments = 6.3-6.4","84 mg/L. ","17 mg/L",0,"across all treatments = 7.5-9.1 mg/L",25,"Controls measured for Fe total and dissolved concentrations. >80% purity Fe2(SO4)3.H2O used"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","163-8"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The effect on the reproduction was followed by the number of neonates produced per adult individual, by counting the total number of neonates produced until either the death of the adult or the end of the experiment.","EC",10,"Non-toxicant Controls","10 replicates","Full Table of acceptability criteria","<24h old neonates","EPA synthetic water","Static, renewal daily.","Measured on Day 1, 4, 7. Total and dissolved. Value given for EC50 is total. Dissolved was<DL.","","Observed","USEPA database software, linear interpolation, probit, dunnets.","95% CLs","Across all treatments = 6.3-6.4","84 mg/L. ","17 mg/L",0,"across all treatments = 7.5-9.1 mg/L",25,"Controls measured for Fe total and dissolved concentrations. >80% purity Fe2(SO4)3.H2O used"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","163-9"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The effect on the reproduction was followed by the number of neonates produced per adult individual, by counting the total number of neonates produced until either the death of the adult or the end of the experiment.","EC",50,"Non-toxicant Controls","10 replicates","Full Table of acceptability criteria","<24h old neonates","EPA synthetic water","Static, renewal daily.","Measured on Day 1, 4, 7. Total and dissolved. Value given for EC50 is total. Dissolved was<DL.","","Observed","USEPA database software, linear interpolation, probit, dunnets.","95% CLs","Across all treatments = 6.3-6.4","84 mg/L. ","17 mg/L",4,"across all treatments = 7.5-8.6 mg/L",25,"Controls measured for Fe total and dissolved concentrations. >80% purity Fe2(SO4)3.H2O used"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","163-10"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The effect on the reproduction was followed by the number of neonates produced per adult individual, by counting the total number of neonates produced until either the death of the adult or the end of the experiment.","EC",10,"Non-toxicant Controls","10 replicates","Full Table of acceptability criteria","<24h old neonates","EPA synthetic water","Static, renewal daily.","Measured on Day 1, 4, 7. Total and dissolved. Value given for EC50 is total. Dissolved was<DL.","","Observed","USEPA database software, linear interpolation, probit, dunnets.","95% CLs","Across all treatments = 6.3-6.4","84 mg/L. ","17 mg/L",4,"across all treatments = 7.5-8.6 mg/L",25,"Controls measured for Fe total and dissolved concentrations. >80% purity Fe2(SO4)3.H2O used"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","163-11"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The effect on the reproduction was followed by the number of neonates produced per adult individual, by counting the total number of neonates produced until either the death of the adult or the end of the experiment.","EC",50,"Non-toxicant Controls","10 replicates","Full Table of acceptability criteria","<24h old neonates","EPA synthetic water","Static, renewal daily.","Measured on Day 1, 4, 7. Total and dissolved. Value given for EC50 is total. Dissolved was<DL.","","Observed","USEPA database software, linear interpolation, probit, dunnets.","95% CLs","Across all treatments = 6.3-6.4","252 mg/L. ","17 mg/L",0,"across all treatments = 8.0-9.2 mg/L",25,"Controls measured for Fe total and dissolved concentrations. >80% purity Fe2(SO4)3.H2O used"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","163-12"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The effect on the reproduction was followed by the number of neonates produced per adult individual, by counting the total number of neonates produced until either the death of the adult or the end of the experiment.","EC",10,"Non-toxicant Controls","10 replicates","Full Table of acceptability criteria","<24h old neonates","EPA synthetic water","Static, renewal daily.","Measured on Day 1, 4, 7. Total and dissolved. Value given for EC50 is total. Dissolved was<DL.","","Observed","USEPA database software, linear interpolation, probit, dunnets.","95% CLs","Across all treatments = 6.3-6.4","252 mg/L. ","17 mg/L",0,"across all treatments = 8.0-9.2 mg/L",25,"Controls measured for Fe total and dissolved concentrations. >80% purity Fe2(SO4)3.H2O used"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","163-13"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The effect on the reproduction was followed by the number of neonates produced per adult individual, by counting the total number of neonates produced until either the death of the adult or the end of the experiment.","EC",50,"Non-toxicant Controls","10 replicates","Full Table of acceptability criteria","<24h old neonates","EPA synthetic water","Static, renewal daily.","Measured on Day 1, 4, 7. Total and dissolved. Value given for EC50 is total. Dissolved was<DL.","","Observed","USEPA database software, linear interpolation, probit, dunnets.","95% CLs","Across all treatments = 6.2-6.4","252 mg/L","17 mg/L",4,"across all treatments = 7.7-9.0 mg/L",25,"Controls measured for Fe total and dissolved concentrations. >80% purity Fe2(SO4)3.H2O used"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","163-14"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The effect on the reproduction was followed by the number of neonates produced per adult individual, by counting the total number of neonates produced until either the death of the adult or the end of the experiment.","EC",10,"Non-toxicant Controls","10 replicates","Full Table of acceptability criteria","<24h old neonates","EPA synthetic water","Static, renewal daily.","Measured on Day 1, 4, 7. Total and dissolved. Value given for EC50 is total. Dissolved was<DL.","","Observed","USEPA database software, linear interpolation, probit, dunnets.","95% CLs","Across all treatments = 6.2-6.4","252 mg/L","17 mg/L",4,"across all treatments = 7.7-9.0 mg/L",25,"Controls measured for Fe total and dissolved concentrations. >80% purity Fe2(SO4)3.H2O used"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","164-1"]
["Scores",10,5,5,5,5,5,0,5,5,4,2,0,0,4,0,3,3,3,0,1,1,3,69,0.69,""]
["Answers","48 h","Immobilisation","LC",50,"non-toxicant controls","Duplicate","","12 ± 12h old","Lake superior water","Static","AAS","","","Litchfield and Wolcoxen 1949","","7.7 (mean) 7.4-8.2 (range)","45.3 mg/L (mean), 44-53 (range)","42.3 (mean), 41-50 (range) mg/L","","9 mg/L","18°C","reagent grade, FeCl3.6H2O"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","164-2"]
["Scores",10,10,5,5,5,5,0,5,5,4,2,0,0,4,0,3,3,3,0,1,1,3,74,0.74,""]
["Answers","21 d","Reproduction. Number of offspring produced each week.","EC",16,"non-toxicant controls","four replicates","","12 ± 12h old","Lake superior water","Static","AAS","","","Litchfield and Wolcoxen 1949","","7.7 (mean) 7.4-8.2 (range)","45.3 mg/L (mean), 44-53 (range)","42.3 (mean), 41-50 (range) mg/L","","9 mg/L","18°C","reagent grade, FeCl3.6H2O"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","164-3"]
["Scores",10,10,5,5,5,5,0,5,5,4,2,0,0,4,0,3,3,3,0,1,1,3,74,0.74,""]
["Answers","21 d","Reproduction. Number of offspring produced each week.","EC",50,"non-toxicant controls","four replicates","","12 ± 12h old","Lake superior water","Static","AAS","","","Litchfield and Wolcoxen 1949","","7.7 (mean) 7.4-8.2 (range)","45.3 mg/L (mean), 44-53 (range)","42.3 (mean), 41-50 (range) mg/L","","9 mg/L","18°C","reagent grade, FeCl3.6H2O"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","164-4"]
["Scores",10,5,5,5,5,5,0,5,5,4,2,0,0,4,0,3,3,3,0,1,1,3,69,0.69,""]
["Answers","21 d","Immobility","LC",50,"non-toxicant controls","four replicates","","12 ± 12h old","Lake superior water","Static","AAS","","","Litchfield and Wolcoxen 1949","","7.7 (mean) 7.4-8.2 (range)","45.3 mg/L (mean), 44-53 (range)","42.3 (mean), 41-50 (range) mg/L","","9 mg/L","18°C","reagent grade, FeCl3.6H2O"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","165-1"]
["Scores",10,10,5,5,5,0,0,5,5,4,4,0,0,4,4,3,3,3,0,0,1,3,74,0.74,""]
["Answers","96 h","Death was defined at the point where ventilation had ceased, the fish were overturned and unresponsive to external stimuli.","LC",50,"non-toxicant controls","10 fish per aquaria in a flow through system.","","15-30 g","Nottingham mains water. Analysis of constituents given.","Flow through","Measured throughout for both total and dissolved","","Table of LC50s provided but no description of concentrations response curve.","Statistical analyses recommended by Fowler & Cohen (1993) were carried out where appropriate using Student’s t-test and two-way analysis of variance (ANOVA) for lethal tests, and the Mann–Whitney U-test for sublethal tests.","95% CLs","6.23 in Controls but ranged from 5.74-6.27 in Fe treatments.","287 mg CaCO3/L in Controls. measured throughout exposure","132 mg CaCO3/L in Controls. measured throughout exposure","","","15°C","AR Grade Fe(III) solution produced from oxidising iron (II) sulfate in AR grade sulphuric acid and AR grade hyudrogen peroxide. Controls also measured for metals."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","165-2"]
["Scores",10,10,5,5,5,0,0,5,5,4,4,0,0,4,4,3,3,3,0,0,1,3,74,0.74,""]
["Answers","96 h","Death was defined at the point where ventilation had ceased, the fish were overturned and unresponsive to external stimuli.","LC",50,"non-toxicant controls","10 fish per aquaria in a flow through system.","","15-30 g","Nottingham mains water. Analysis of constituents given.","Flow through","Measured throughout for both total and dissolved","","Table of LC50s provided but no description of concentrations response curve.","Statistical analyses recommended by Fowler & Cohen (1993) were carried out where appropriate using Student’s t-test and two-way analysis of variance (ANOVA) for lethal tests, and the Mann–Whitney U-test for sublethal tests.","95% CLs","6.23 in Controls but ranged from 5.74-6.27 in Fe treatments.","287 mg CaCO3/L in Controls. measured throughout exposure","132 mg CaCO3/L in Controls. measured throughout exposure","","","15°C","AR Grade Fe(III) solution produced from oxidising iron (II) sulfate in AR grade sulphuric acid and AR grade hyudrogen peroxide. Controls also measured for metals."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","166-1"]
["Scores",10,5,0,5,5,0,2,5,5,4,0,0,4,0,0,3,1,0,0,3,3,3,58,0.57999999999999996,"The highest possible grade for this datum is acceptable."]
["Answers","14 d","Reproduction, progeny per female. Referred to previous publication for details Dave, 1984.","","NOEC can be estimated from data in Tables","Non-toxicant controls","Not stated","Control data presented in Tables. >90% surviavl in 14d, >80% survival in 21 d.","6-24h old","Reconsitituted water (ISO, 1982) supplemented with 1 in 25 parts seawter","Static renewal, Mon, Wed, Fri renewal.","","","Observable from data in Tables.","No statistics","","7.0-8.0 throughout test","250 mg/L CaCO3","","","50-100% saturation","20.6 ± 0.9 °C","pro anlaysi = reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","166-2"]
["Scores",10,5,0,5,5,0,2,5,5,4,0,0,4,0,0,3,1,0,0,3,3,3,58,0.57999999999999996,"The highest possible grade for this datum is acceptable."]
["Answers","14 d","Reproduction, progeny per female. Referred to previous publication for details Dave, 1984.","","NOEC can be estimated from data in Tables","Non-toxicant controls","Not stated","Control data presented in Tables. >90% surviavl in 14d, >80% survival in 21 d.","6-24h old","Reconsitituted water (ISO, 1982) supplemented with 1 in 25 parts seawter","Static renewal, Mon, Wed, Fri renewal.","","","Observable from data in Tables.","No statistics","","7.0-8.0 throughout test","250 mg/L CaCO3","","","50-100% saturation","20.6 ± 0.9 °C","pro anlaysi = reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","167-1"]
["Scores",10,5,0,0,5,0,0,5,5,4,0,0,0,0,0,3,1,0,0,1,1,3,43,0.43,"The highest possible grade for this datum is acceptable."]
["Answers","16 days","Survival, hatchability","","Data on graphs but impoosible to determine any toxicity values.","non-toxicant controls","","","eggs, 1.1-1.2 days post-spawning","Aerated aged municipal water. Constituents described.","Static renewal, daily.","","","No concentration response observable","log probit paper. And 'microcomputer according to Davies, 1991'","","article investigated effects of pH on metal toxicity, so several buffesr used and pH adjusted and measured throughout. Diluent (7.4-7.9), 5 (5.0-5.4), 6 (6.0-7.4), 7 (7-7.5), 8 (7.8-8.0), 9 (8.0-9.0).","40 mg CaCO3/L","","","Stated to be 'close to saturation'","25°C","AR Grade FeCl3.6H2O"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","169-1"]
["Scores",10,10,5,5,5,5,0,5,5,4,2,0,4,4,4,1,0,0,0,0,1,0,70,0.7,""]
["Answers","40 min","Percentage of eyes-eggs 10 days after fertilsiation test (eggs were transferred from exposure concentrations to diluent) were counted to represent fertilisation rate.","Stated as concentration at which effect occurred (i.e. akin to LOEC), so NOEC taken to be next concentration down in the series","LOEC data can be determined from tabulkated data of 'concentration at which metals affect... (ovum, sperm or fertilisation)'","Diluent controls","duplicate","","sperm diluted using 1 in 10","Conservation diluent: NaCl diluted in water to somotic pressure of 250mOsm/kg plus added KCl","Static","Dissolved and total Fe measured.","","Stated and observed in graphs","variance anlayses, transformation. (ANOVA assumed)","tested at 0.05, 0.01, 0.005",9,"","","","","10 ± 1°C","not stated"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","169-2"]
["Scores",10,10,5,5,5,5,0,5,5,4,2,0,4,4,4,1,0,0,0,0,1,0,70,0.7,""]
["Answers","40 min","Percentage of eyes-eggs 10 days after fertilsiation test (eggs were transferred from exposure concentrations to diluent) were counted to represent fertilisation rate.","Stated as concentration at which effect occurred (i.e. akin to LOEC), so NOEC taken to be next concentration down in the series","LOEC data can be determined from tabulkated data of 'concentration at which metals affect... (ovum, sperm or fertilisation)'","Diluent controls","duplicate","","sperm diluted using 1 in 100","Conservation diluent: NaCl diluted in water to somotic pressure of 250mOsm/kg plus added KCl","Static","Dissolved and total Fe measured.","","Stated and observed in graphs","variance anlayses, transformation. (ANOVA assumed)","tested at 0.05, 0.01, 0.005",9,"","","","","10 ± 1°C","not stated"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","169-3"]
["Scores",10,10,5,5,5,5,0,0,5,4,2,0,4,4,4,1,0,0,0,0,1,0,65,0.65,""]
["Answers","40 min","Percentage of eyes-eggs 10 days after fertilsiation test (eggs were transferred from exposure concentrations to diluent) were counted to represent fertilisation rate.","Stated as concentration at which effect occurred (i.e. akin to LOEC), so NOEC taken to be next concentration down in the series","LOEC data can be determined from tabulkated data of 'concentration at which metals affect... (ovum, sperm or fertilisation)'","Diluent controls","duplicate","","","Conservation diluent: NaCl diluted in water to somotic pressure of 250mOsm/kg plus added KCl","Static","Dissolved and total Fe measured.","","Stated and observed in graphs","variance anlayses, transformation. (ANOVA assumed)","tested at 0.05, 0.01, 0.005",9,"","","","","10 ± 1°C","not stated"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","170-1"]
["Scores",10,5,5,5,5,5,2,0,5,0,0,4,0,4,0,1,1,0,0,0,1,3,56,0.56000000000000005,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Immobilisation","EC",84,"Diluent controls","duplicate","Reference toxicant toxicity reported, no immobility on controls, reference to ISO 1996 method.","States 'newborn'","ISO reconstituted water (ISO 1996), softwater","","","potassium dichromate 24h EC50 was 1.1 mg/L","not stated","EC84 values for Daphnia magna (48-h immobility) were determined approximately or by the moving average method according to Peltier and Weber (1985). An ANOVA test was performed to determine the impact of metal (N = 5), replicate (N = 2) treatments (N = 8) on bioassay responses. \n","","7.2-7.6","45 mg CaCO3/L","","","","22 ± 2°C","AR Grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","170-2"]
["Scores",10,5,5,5,5,5,2,0,5,0,0,4,0,4,0,1,1,0,0,0,1,3,56,0.56000000000000005,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Immobilisation","EC",84,"Diluent controls","duplicate","Reference toxicant toxicity reported, no immobility on controls, reference to ISO 1996 method.","States 'newborn'","ISO reconstituted water (ISO 1996), hard water","","","potassium dichromate 24h EC50 was 1.1 mg/L","not stated","EC84 values for Daphnia magna (48-h immobility) were determined approximately or by the moving average method according to Peltier and Weber (1985). An ANOVA test was performed to determine the impact of metal (N = 5), replicate (N = 2) treatments (N = 8) on bioassay responses. \n","","8.0-8.2","250 mg CaCO3/L","","","","22 ± 2°C","AR Grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","171-1"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,1,1,0,1,3,0,67,0.67,""]
["Answers","96 h","Survival","Stated as 'LD', however by current descriptions is equivalent to LC. Described in the text as 'concentration at which 50% of the test fish survival'.",50,"Diluent controls","Large flow through system so only pseudoreplication possible. 10 fish per concentration","APHA method 1971, Control survival also reported for each test (100%).","14 months old, 210 mm total length, 130 g","Filtered water from Tygart River, via the City of Elkins, West Virginia. Carbon filtered and refrigeration system to maintain temperature.","Flow through, 90% exchange every 9h.","Measured every 24 h. ","","Observable from tables and graphs.","Graphical method.","",7,"Measured but not stated","Measured but not stated","","Measured but not stated","11°C","Not stated. Salt used was FeSO4."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","171-2"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,1,1,0,1,3,0,67,0.67,""]
["Answers","96 h","Survival","Stated as 'LD', however by current descriptions is equivalent to LC. Described in the text as 'concentration at which 50% of the test fish survival'.",50,"Diluent controls","Large flow through system so only pseudoreplication possible. 10 fish per concentration","APHA method 1971, Control survival also reported for each test (100%).","14 months old, 210 mm total length, 130 g","Filtered water from Tygart River, via the City of Elkins, West Virginia. Carbon filtered and refrigeration system to maintain temperature.","Flow through, 90% exchange every 9h.","Measured every 24 h. ","","Observable from tables and graphs.","Graphical method.","",6,"Measured but not stated","Measured but not stated","","Measured but not stated","11°C","Not stated. Salt used was FeSO4."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","171-3"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,1,1,0,1,3,0,67,0.67,""]
["Answers","96 h","Survival","Stated as 'LD', however by current descriptions is equivalent to LC. Described in the text as 'concentration at which 50% of the test fish survival'.",50,"Diluent controls","Large flow through system so only pseudoreplication possible. 10 fish per concentration","APHA method 1971, Control survival also reported for each test (100%).","14 months old, 210 mm total length, 130 g","Filtered water from Tygart River, via the City of Elkins, West Virginia. Carbon filtered and refrigeration system to maintain temperature.","Flow through, 90% exchange every 9h.","Measured every 24 h. ","","Observable from tables and graphs.","Graphical method.","",5.5,"Measured but not stated","Measured but not stated","","Measured but not stated","11°C","Not stated. Salt used was FeSO4."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","172-1"]
["Scores",10,5,5,5,5,5,2,5,5,4,0,0,0,4,4,3,1,0,0,3,0,0,66,0.66,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Survival","LC",50,"Diluent controls","duplicate, also test repeated","EPA method, Peltier and Weber 1990","<24h old","Moderately hard synthetic water","Static non-renewal","","","Not stated","EPA probit analysis Peltier and Weber 1990","95% CLs of 26610-46910","7.0-7.3 in all dilutions","Not stated, but EPA method and recipe stated - which would make water with hardness 80-100 mg CaCO3/L","","","7.8-8.2 mg/L in all treatments","","Commercial grade. Not measured."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","172-2"]
["Scores",10,5,5,5,5,5,2,5,5,4,0,0,0,4,4,3,1,0,0,3,0,0,66,0.66,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Survival","LC",50,"Diluent controls","duplicate, also test repeated","EPA method, Peltier and Weber 1990","<24h old","Moderately hard synthetic water","Static non-renewal","","","Not stated","EPA probit analysis Peltier and Weber 1990","95% CLs of 26610-46910","7.0-7.3 in all dilutions","Not stated, but EPA method and recipe stated - which would make water with hardness 80-100 mg CaCO3/L","","","7.8-8.2 mg/L in all treatments","","Commercial grade. Not measured."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","173-1"]
["Scores",10,5,0,5,5,0,0,5,0,4,2,0,4,0,0,3,0,0,0,3,1,3,45,0.5,""]
["Answers","30 days","Survival","","From figures.","Diluent control","Not stated. 60 individuals exposed to each concentration, but not reported how the expsures were done.","","Larvae","Just states 'stream water'","Water renewed weekly","Measured throughout, but not all values reported","","","","","4.5 ± 0.3","","","","60-70% in all treatments","11 ± 2°C","Grade not listed, but Controls measured."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","173-2"]
["Scores",10,5,0,5,5,0,0,5,0,4,2,0,4,0,0,3,0,0,0,3,1,3,45,0.5,""]
["Answers","30 days","Survival","","From figures.","Diluent control","Not stated. 60 individuals exposed to each concentration, but not reported how the expsures were done.","","Larvae","Just states 'stream water'","Water renewed weekly","Measured throughout, but not all values reported","","","","","7.5 ± 0.7","","","","60-70% in all treatments","11 ± 2°C","Grade not listed, but Controls measured."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","173-3"]
["Scores",10,10,0,5,5,0,0,0,0,4,2,0,4,0,0,3,0,0,0,3,1,3,50,0.5,""]
["Answers","30 days","measured as locomotion after mechanical stimulas (yes/no)","","From figures.","Diluent control","Not stated. 60 individuals exposed to each concentration, but not reported how the expsures were done.","","","Just states 'stream water'","Water renewed weekly","Measured throughout, but not all values reported","","","","","4.5 ± 0.3","","","","60-70% in all treatments","11 ± 2°C","Grade not listed, but Controls measured."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","173-4"]
["Scores",10,10,0,5,5,0,0,0,0,4,2,0,4,0,0,3,0,0,0,3,1,3,50,0.5,""]
["Answers","30 days","measured as locomotion after mechanical stimulas (yes/no)","","From figures.","Diluent control","Not stated. 60 individuals exposed to each concentration, but not reported how the expsures were done.","","","Just states 'stream water'","Water renewed weekly","Measured throughout, but not all values reported","","","","","7.5 ± 0.7","","","","60-70% in all treatments","11 ± 2°C","Grade not listed, but Controls measured."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","174-1"]
["Scores",10,5,5,5,5,5,0,0,5,4,2,0,4,4,4,3,0,0,3,0,1,3,68,0.68,""]
["Answers","96 h","Survival","LC",50,"Diluent Controls","Duplicate","","","Unfiltered streamwater, phys-chem measured.","Static, renewed daily","Measured throughout, but not used in calculation of LC50.","","Graphs","","95% CLs 111300-101500","6.1-6.7, measured throughout in all treatments","","","21.6 mg CaCO3/L","","10 ± 1°C","Grade not described, but controls measured. FeSO4.7H2) salt used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","174-2"]
["Scores",10,5,5,5,5,5,0,0,5,4,2,0,4,4,4,3,0,0,3,0,1,3,68,0.68,""]
["Answers","5 d","Survival","LC",50,"Diluent Controls","Duplicate","","","Unfiltered streamwater, phys-chem measured.","Static, renewed daily","Measured throughout, but not used in calculation of LC50.","","Graphs","","95% CLs 111300-101500","6.1-6.7, measured throughout in all treatments","","","21.6 mg CaCO3/L","","10 ± 1°C","Grade not described, but controls measured. FeSO4.7H2) salt used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","174-3"]
["Scores",10,10,5,5,5,5,0,0,5,4,2,0,4,4,4,3,0,0,3,0,1,3,73,0.73,""]
["Answers","96 h","if the mayflies moved a distance of more than one body length away from the stimulus, irrespective of the kind of movement (swimming, creeping).","EC",50,"Diluent Controls","Duplicate","","","Unfiltered streamwater, phys-chem measured.","Static, renewed daily","Measured throughout, but not used in calculation of LC50.","","Graphs","","95% CLs 47100-103900","6.1-6.7, measured throughout in all treatments","","","21.6 mg CaCO3/L","","10 ± 1°C","Grade not described, but controls measured. FeSO4.7H2) salt used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","174-4"]
["Scores",10,10,5,5,5,5,0,0,5,4,2,0,4,4,4,3,0,0,3,0,1,3,73,0.73,""]
["Answers","5 d","if the mayflies moved a distance of more than one body length away from the stimulus, irrespective of the kind of movement (swimming, creeping).","EC",50,"Diluent Controls","Duplicate","","","Unfiltered streamwater, phys-chem measured.","Static, renewed daily","Measured throughout, but not used in calculation of LC50.","","Graphs","","95% CLs 47100-103900","6.1-6.7, measured throughout in all treatments","","","21.6 mg CaCO3/L","","10 ± 1°C","Grade not described, but controls measured. FeSO4.7H2) salt used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","174-5"]
["Scores",10,5,5,5,5,5,0,0,5,4,2,0,4,4,4,3,0,0,3,0,1,3,68,0.68,""]
["Answers","96 h","survival","LC",50,"Diluent Controls","Duplicate","","","Unfiltered streamwater, phys-chem measured.","Static, renewed daily","Measured throughout, but not used in calculation of LC50.","","Graphs","","95% CLs 78500-101100","4.4-4.6, measured throughout in all treatments","","","21.6 mg CaCO3/L","","10 ± 1°C","Grade not described, but controls measured. FeSO4.7H2) salt used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","174-6"]
["Scores",10,5,5,5,5,5,0,0,5,4,2,0,4,4,4,3,0,0,3,0,1,3,68,0.68,""]
["Answers","5d","survival","LC",50,"Diluent Controls","Duplicate","","","Unfiltered streamwater, phys-chem measured.","Static, renewed daily","Measured throughout, but not used in calculation of LC50.","","Graphs","","95% CLs 64900-65700","4.4-4.6, measured throughout in all treatments","","","21.6 mg CaCO3/L","","10 ± 1°C","Grade not described, but controls measured. FeSO4.7H2) salt used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","174-7"]
["Scores",10,10,5,5,5,5,0,0,5,4,2,0,4,4,4,3,0,0,3,0,1,3,73,0.73,""]
["Answers","96 h","if the mayflies moved a distance of more than one body length away from the stimulus, irrespective of the kind of movement (swimming, creeping).","LC",50,"Diluent Controls","Duplicate","","","Unfiltered streamwater, phys-chem measured.","Static, renewed daily","Measured throughout, but not used in calculation of LC50.","","Graphs","","95% CLs 51000-80200","4.4-4.6, measured throughout in all treatments","","","21.6 mg CaCO3/L","","10 ± 1°C","Grade not described, but controls measured. FeSO4.7H2) salt used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","174-8"]
["Scores",10,10,5,5,5,5,0,0,5,4,2,0,4,4,4,3,0,0,3,0,1,3,73,0.73,""]
["Answers","5 d","if the mayflies moved a distance of more than one body length away from the stimulus, irrespective of the kind of movement (swimming, creeping).","EC",50,"Diluent Controls","Duplicate","","","Unfiltered streamwater, phys-chem measured.","Static, renewed daily","Measured throughout, but not used in calculation of LC50.","","Graphs","","95% CLs 24200-66700","4.4-4.6, measured throughout in all treatments","","","21.6 mg CaCO3/L","","10 ± 1°C","Grade not described, but controls measured. FeSO4.7H2) salt used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","175-1"]
["Scores",10,0,5,5,5,5,2,5,5,0,0,4,0,4,4,1,1,1,0,0,1,3,61,0.61,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","","LC",50,"Diluent controls","duplicate and repeated 3 times","ROTOXKIT methods","Cysts hatched and used 18-20 h later","EPA media","Assume static","","potassium dichromate","","Trimmed spearman karber","95% CLs (± 45900)","Adjusted to 5.5 ± 0.2","Based on EPA medium, hardness is 80-100 mg CaCO3/L","Based on EPA medium, hardness is 60-70 mg CaCO3/L","","","25 ± 1°C","AR Grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","175-2"]
["Scores",10,0,5,5,5,5,2,5,5,0,0,4,0,4,4,0,1,1,0,0,1,3,60,0.6,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","","LC",50,"Diluent controls","duplicate and repeated 3 times","STREPTOXKIT methods","Instar II-III","EPA media","Assume static","","potassium dichromate","","Trimmed spearman karber","95% CLs (± 46000)","Just states no pH adjustment done","Based on EPA medium, hardness is 80-100 mg CaCO3/L","Based on EPA medium, hardness is 60-70 mg CaCO3/L","","","25 ± 1°C","AR Grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","176-1"]
["Scores",10,0,5,5,5,5,0,0,0,4,0,0,0,0,0,0,0,0,0,0,1,0,35,0.35,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",50,"Diluent controls","triplicate","","","","Static non-renewal","","","","Unclear","","","","","","","20°C","reported as mg/L Fe+++"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","176-2"]
["Scores",10,0,5,5,5,5,0,0,0,4,0,0,0,0,0,0,0,0,0,0,1,0,35,0.35,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",50,"Diluent controls","triplicate","","","","Static non-renewal","","","","Unclear","","","","","","","20°C","reported as mg/L Fe+++"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","176-3"]
["Scores",10,0,5,5,5,5,0,0,0,4,0,0,0,0,0,0,0,0,0,0,1,0,35,0.35,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",50,"Diluent controls","triplicate","","","","Static non-renewal","","","","Unclear","","","","","","","20°C","reported as mg/L Fe+++"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","177-1"]
["Scores",10,10,5,5,5,5,0,0,5,4,4,0,4,4,4,1,1,0,0,0,0,3,70,0.7,""]
["Answers","24 days","positive escape reaction was recorded if the mayflies moved more than one body length away from the stimulus","EC",50,"Diluent controls","duplicate","","","streamwater - pH, hardness Cd and Fe content characterised.","static renewal. Renewed twice per week.","Measured throughout","","","","95% CLs 14700-16700","4.5 ± 0.2. adjusted to this pH daily.","0.27 mM","","","","","Control measured for Fe content."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","178-1"]
["Scores",10,10,5,5,5,5,0,5,5,4,2,0,0,4,4,3,0,0,0,0,1,0,68,0.68,""]
["Answers","96 h","A fish was considered dead when opercular movement had ceased and they no longer responded to prodding.","LC",50,"Diluent controls","duplicate","","yearling","artificial test water - 250 µM K+ and Ca2+, and 450 µM Na+","flow through","Measured throughout, but not reported anywhere. Unsure if LC50s are total or dissolved or nominal","","","probit","95% CLs","5.8-6.0","Ca = 0.05 mM","","","","14-15°C","Not stated"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","178-2"]
["Scores",10,10,5,5,5,5,0,5,5,4,2,0,0,4,4,3,0,0,0,0,1,0,68,0.68,""]
["Answers","96 h","A fish was considered dead when opercular movement had ceased and they no longer responded to prodding.","LC",50,"Diluent controls","duplicate","","yearling","artificial test water - 250 µM K+ and Ca2+, and 450 µM Na+","flow through","Measured throughout, but not reported anywhere. Unsure if LC50s are total or dissolved or nominal","","","probit","95% CLs","5.8-6.0","Ca = 0.25 mM","","","","14-15°C","Not stated"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","178-3"]
["Scores",10,10,5,5,5,5,0,5,5,4,2,0,0,4,4,3,0,0,0,0,1,0,68,0.68,""]
["Answers","96 h","A fish was considered dead when opercular movement had ceased and they no longer responded to prodding.","LC",50,"Diluent controls","duplicate","","yearling","artificial test water - 250 µM K+ and Ca2+, and 450 µM Na+","flow through","Measured throughout, but not reported anywhere. Unsure if LC50s are total or dissolved or nominal","","","probit","95% CLs","5.8-6.0","Ca = 2.5 mM","","","","14-15°C","Not stated"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","179-1"]
["Scores",10,5,5,5,5,0,2,5,0,4,0,0,0,4,0,0,0,0,0,0,1,0,46,0.46,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Survival","LC",50,"Diluent controls","not stated. Although followed USEPA methods so should be","USEPA methods","<24h old","DMW and hardwater stated - but not clear which was used for  Fe exposures","static","","","","","","But stated that pH was low where mortality seen and likley not effect of Fe, rather pH.","Unclear if DMW or Hardwater","Unclear if DMW or Hardwater","Unclear if DMW or Hardwater","","25°C",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","180-3"]
["Scores",10,5,0,5,5,0,2,0,5,0,0,0,4,4,0,0,0,0,0,0,0,0,40,0.4,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Survival","","From table","non toxicant control","only at some concentrations","EPA method","","EPA medium","","","","From figures","","not stated","7.5-8.1, however, unclear if this is in Fe tests or nanoFe tests only. Table of data for Nano-Fe only. No pH data for Fe exposure","","","","","",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","180-4"]
["Scores",10,5,0,5,5,0,2,0,5,0,0,0,4,4,0,0,0,0,0,0,0,0,40,0.4,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Survival","","From table","non toxicant control","only at some concentrations","EPA method","","EPA medium","","","","From figures","","not stated","7.5-8.1, however, unclear if this is in Fe tests or nanoFe tests only. Table of data for Nano-Fe only. No pH data for Fe exposure","","","","","",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","181-1"]
["Scores",10,10,5,5,5,5,2,5,5,4,0,0,0,4,4,3,0,0,0,3,3,3,76,0.76,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","Mortality, defined as lack of movement upon gentle prodding","LC",50,"Diluent Controls","triplicate","ASTM methods 1998","4-week old juveniles","Carbon filtered water","static non-renewal","","","","","95% CLs (43000-58000)","7±1","","","","8±0.5 mg/L","21 ± 2","AR grade chemicals"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","181-2"]
["Scores",10,10,5,5,5,5,2,5,5,4,0,0,0,4,4,3,0,0,0,3,3,3,76,0.76,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","Mortality, defined as lack of movement upon gentle prodding","LC",50,"Diluent Controls","triplicate","ASTM methods 1998","4-week old juveniles","Carbon filtered water","static non-renewal","","","","","95% CLs (61000-84000)","7±1","","","","8±0.5 mg/L","21 ± 2","AR grade chemicals"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","181-3"]
["Scores",10,10,5,5,5,5,2,5,5,4,0,0,0,4,4,3,0,0,0,3,3,3,76,0.76,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","Mortality, defined as lack of movement upon gentle prodding","LC",50,"Diluent Controls","triplicate","ASTM methods 1998","4-week old juveniles","Carbon filtered water","static non-renewal","","","","","95% CLs (97000155000)","7±1","","","","8±0.5 mg/L","21 ± 2","AR grade chemicals"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","181-4"]
["Scores",10,10,5,5,5,5,2,5,5,4,0,0,0,4,4,3,0,0,0,3,3,3,76,0.76,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","Mortality, defined as lack of movement upon gentle prodding","LC",50,"Diluent Controls","triplicate","ASTM methods 1998","4-week old juveniles","Carbon filtered water","static non-renewal","","","","","95% CLs (141000-287000)","7±1","","","","8±0.5 mg/L","21 ± 2","AR grade chemicals"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","182-1"]
["Scores",10,10,5,5,5,5,2,0,5,4,0,0,0,4,4,3,3,3,0,0,3,3,74,0.74,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Test worms were considered dead when there was complete immobilization and no response to pressing with a blunt glass rod. Death was further confirmed by transferring worms back to fresh control tubewell water.","LC",50,"Tubewell water","triplicate","APHA method, also: 'In control tests, tubificid worms remained active during the test period.","","Tubewell water, phys-chem described","renewed every 24h ","","","","","95% CLs (102500-123680)","30°C mean (control water only)","245 mg CaCO3/L","400 mg CaCO3/L","","","30 °C (range 29.5-31)","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","182-2"]
["Scores",10,10,5,5,5,5,2,0,5,4,0,0,0,4,4,3,3,3,0,0,3,3,74,0.74,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Test worms were considered dead when there was complete immobilization and no response to pressing with a blunt glass rod. Death was further confirmed by transferring worms back to fresh control tubewell water.","LC",50,"Tubewell water","triplicate","APHA method, also: 'In control tests, tubificid worms remained active during the test period.","","Tubewell water, phys-chem described","renewed every 24h ","","","","","95% CLs (91830-111570)","30°C mean (control water only)","245 mg CaCO3/L","400 mg CaCO3/L","","","30 °C (range 29.5-31)","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","182-3"]
["Scores",10,10,5,5,5,5,2,0,5,4,0,0,0,4,4,3,3,3,0,0,3,3,74,0.74,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Test worms were considered dead when there was complete immobilization and no response to pressing with a blunt glass rod. Death was further confirmed by transferring worms back to fresh control tubewell water.","LC",50,"Tubewell water","triplicate","APHA method, also: 'In control tests, tubificid worms remained active during the test period.","","Tubewell water, phys-chem described","renewed every 24h ","","","","","95% CLs (91830-111570)","30°C mean (control water only)","245 mg CaCO3/L","400 mg CaCO3/L","","","30 °C (range 29.5-31)","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","183-1"]
["Scores",10,10,5,5,5,5,2,0,5,4,0,4,0,4,4,3,3,3,0,3,3,3,81,0.81,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Immobility, i.e. the inhibition to swim within 15 second after gently agitation of the test  container. Immobility implies the organism usually swim helplessly at the bottom of the container and use their limbs to crawled with difficulty.","Lc",50,"Diluent Controls","triplicate and test repeated","In control tests ostracods remain active throughout test period.","","Well water and physico-chem described.","static renewal, 24h renewal.","","","not stated. Only LC50s tabulated","moving average angle method","95% CLs (193600-252000)","7.4-7.7 (Control water)","245 (230-250) mg CaCO3/L","400 (390-410) mg CaCO3/L","","5.4 (5.1-6.1) mg/L","21 (20-22)","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","183-2"]
["Scores",10,10,5,5,5,5,2,0,5,4,0,4,0,4,4,3,3,3,0,3,3,3,81,0.81,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Immobility, i.e. the inhibition to swim within 15 second after gently agitation of the test  container. Immobility implies the organism usually swim helplessly at the bottom of the container and use their limbs to crawled with difficulty.","Lc",50,"Diluent Controls","triplicate and test repeated","In control tests ostracods remain active throughout test period.","","Well water and physico-chem described.","static renewal, 24h renewal.","","","not stated. Only LC50s tabulated","moving average angle method","95% CLs (98510-130000)","7.4-7.7 (Control water)","245 (230-250) mg CaCO3/L","400 (390-410) mg CaCO3/L","","5.4 (5.1-6.1) mg/L","21 (20-22)","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","184-1"]
["Scores",10,10,5,5,5,5,0,0,5,4,0,0,0,4,4,3,3,3,0,3,3,3,75,0.75,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","dead when there was complete immobilization (no heart beat) and no response to pressing with a blunt glass rod. Death was further confirmed by transferring daphnids back to fresh control tubewell water.","LC",50,"Diluent controls","triplicate","","","Tubewell water, phys-chem described","Static","","","","moving angle average","95% CLs (19850-32340)","7.6 (.2-7.8) in control water","240 (235-260) mg CaCO3/L","400 (390-415) mg CaCO3/L","","5.6 (5.2-6.5) mg/L","13 (11.5-14.5)","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","184-2"]
["Scores",10,10,5,5,5,5,0,0,5,4,0,0,0,4,4,3,3,3,0,3,3,3,75,0.75,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","dead when there was complete immobilization (no heart beat) and no response to pressing with a blunt glass rod. Death was further confirmed by transferring daphnids back to fresh control tubewell water.","LC",50,"Diluent controls","triplicate","","","Tubewell water, phys-chem described","Static","","","","moving angle average","95% CLs (6150-9260)","7.6 (.2-7.8) in control water","240 (235-260) mg CaCO3/L","400 (390-415) mg CaCO3/L","","5.6 (5.2-6.5) mg/L","13 (11.5-14.5)","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","185-1"]
["Scores",10,5,5,5,5,5,0,5,5,4,0,0,0,4,4,3,3,3,0,3,3,3,75,0.75,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Mortality","LC",50,"Diluent controls","triplicate","","averaged 20 mm (range of 15 - 25 mm) in length and 500 mg (350 - 800 nig) in wet weight","Name not provided but detailed physico-chemistry provided","24h renewals","","","","moving agerage angle method","95% CLs (23250-26540)","6.1 (6.1-6.7) - all treatments","20 (13-80) mg CaCO3/L","27 (24-40) mg CaCO3/L","","6.4 (6.2-7.0) mg/L","15 (13-16)°C","Reagent grade, FeSO4.7H2O"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","185-2"]
["Scores",10,5,5,5,5,5,0,5,5,4,0,0,0,4,4,3,3,3,0,3,3,3,75,0.75,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Mortality","LC",50,"Diluent controls","triplicate","","averaged 20 mm (range of 15 - 25 mm) in length and 500 mg (350 - 800 nig) in wet weight","Name not provided but detailed physico-chemistry provided","24h renewals","","","","moving agerage angle method","95% CLs (20690-24010)","6.1 (6.1-6.7) - all treatments","20 (13-80) mg CaCO3/L","27 (24-40) mg CaCO3/L","","6.4 (6.2-7.0) mg/L","15 (13-16)°C","Reagent grade, FeSO4.7H2O"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","185-3"]
["Scores",10,5,5,5,5,5,0,5,5,4,0,0,0,4,4,3,3,3,0,3,3,3,75,0.75,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Mortality","LC",50,"Diluent controls","triplicate","","averaged 20 mm (range of 15 - 25 mm) in length and 500 mg (350 - 800 nig) in wet weight","Name not provided but detailed physico-chemistry provided","24h renewals","","","","moving agerage angle method","95% CLs (18430-21180)","6.1 (6.1-6.7) - all treatments","20 (13-80) mg CaCO3/L","27 (24-40) mg CaCO3/L","","6.4 (6.2-7.0) mg/L","15 (13-16)°C","Reagent grade, FeSO4.7H2O"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","185-4"]
["Scores",10,5,5,5,5,5,0,5,5,4,0,0,0,4,4,3,3,3,0,3,3,3,75,0.75,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Mortality","LC",50,"Diluent controls","triplicate","","averaged 20 mm (range of 15 - 25 mm) in length and 500 mg (350 - 800 nig) in wet weight","Name not provided but detailed physico-chemistry provided","24h renewals","","","","moving agerage angle method","95% CLs (16390-18320)","6.1 (6.1-6.7) - all treatments","20 (13-80) mg CaCO3/L","27 (24-40) mg CaCO3/L","","6.4 (6.2-7.0) mg/L","15 (13-16)°C","Reagent grade, FeSO4.7H2O"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","186-1"]
["Scores",10,10,5,5,5,0,0,0,0,0,0,0,4,0,0,0,0,0,0,0,1,3,43,0.43,"The highest possible grade for this datum is acceptable."]
["Answers","14 d","A dead nematode was defined as an immobile one which gave no response to mechanical stimuli with a needle.","LC (although named LD)",50,"distilled water controls","not stated","","","","","","","Graph","","","","","","","","25°C","Analytical grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-1"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,94,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","NOEC","0.05 significance level","Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","0.05 significance level","pH-adjusted each day to pH 6","12 mg/L CaCO3","116 mg CaCO3/L","354 mg/L (nominal 0, but buffer added and this brought up DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-2"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,94,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","LOEC","0.05 significance level","Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","0.05 significance level","pH-adjusted each day to pH 6","12 mg/L CaCO3","116 mg CaCO3/L","354 mg/L (nominal 0, but buffer added and this brought up DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-3"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,94,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",10,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs (13.1-2820.7","pH-adjusted each day to pH 6","12 mg/L CaCO3","116 mg CaCO3/L","354 mg/L (nominal 0, but buffer added and this brought up DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-4"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,94,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",20,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs (34.8-3943.4)","pH-adjusted each day to pH 6","12 mg/L CaCO3","116 mg CaCO3/L","354 mg/L (nominal 0, but buffer added and this brought up DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-5"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,94,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",50,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs (381-18582)","pH-adjusted each day to pH 6","12 mg/L CaCO3","116 mg CaCO3/L","354 mg/L (nominal 0, but buffer added and this brought up DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-6"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,93,0.93,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","NOEC","0.05 significance","Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","",0.05,"pH-adjusted each day to pH 6","8 mg/L CaCO3","120 mg CaCO3/L","measured without buffer m= 1.7 mg/L, but all other solutions show that buffer added 350 mg/L DOC",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-6"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,93,0.93,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","LOEC","0.05 significance","Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","",0.05,"pH-adjusted each day to pH 6","8 mg/L CaCO3","120 mg CaCO3/L","measured without buffer m= 1.7 mg/L, but all other solutions show that buffer added 350 mg/L DOC",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-7"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,93,0.93,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",10,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs (72.8-1184.5)","pH-adjusted each day to pH 6","8 mg/L CaCO3","120 mg CaCO3/L","measured without buffer m= 1.7 mg/L, but all other solutions show that buffer added 350 mg/L DOC",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-8"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,93,0.93,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",20,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs (674-3133.8))","pH-adjusted each day to pH 6","8 mg/L CaCO3","120 mg CaCO3/L","measured without buffer m= 1.7 mg/L, but all other solutions show that buffer added 350 mg/L DOC",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-9"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,93,0.93,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",50,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs (3464-348111)","pH-adjusted each day to pH 6","8 mg/L CaCO3","120 mg CaCO3/L","measured without buffer m= 1.7 mg/L, but all other solutions show that buffer added 350 mg/L DOC",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-10"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","NOEC","0.05 level","Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","",0.05,"pH-adjusted each day to pH 6","75 mg/L CaCO3","108 mg CaCO3/L","332 mg/L (nominal = 0 mg/L, but buffer used to adjust pH changed DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-11"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","LOEC","0.05 level","Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","",0.05,"pH-adjusted each day to pH 6","75 mg/L CaCO3","108 mg CaCO3/L","332 mg/L (nominal = 0 mg/L, but buffer used to adjust pH changed DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-12"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",10,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs (152.6-654.4)","pH-adjusted each day to pH 6","75 mg/L CaCO3","108 mg CaCO3/L","332 mg/L (nominal = 0 mg/L, but buffer used to adjust pH changed DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-13"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",20,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs (333.6-1025.5)","pH-adjusted each day to pH 6","75 mg/L CaCO3","108 mg CaCO3/L","332 mg/L (nominal = 0 mg/L, but buffer used to adjust pH changed DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-14"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",50,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs (1376-2859)","pH-adjusted each day to pH 6","75 mg/L CaCO3","108 mg CaCO3/L","332 mg/L (nominal = 0 mg/L, but buffer used to adjust pH changed DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-18"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","NOEC","0.05 significance","Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","",0.05,"pH-adjusted each day to pH 6","232 mg/L CaCO3","116 mg CaCO3/L","366 mg/L (nominal = 0 mg/L, but buffer used to adjust pH changed DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-19"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","LOEC","0.05 significance","Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","",0.05,"pH-adjusted each day to pH 6","232 mg/L CaCO3","116 mg CaCO3/L","366 mg/L (nominal = 0 mg/L, but buffer used to adjust pH changed DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-20"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",10,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs (21.8-5061.7)","pH-adjusted each day to pH 6","232 mg/L CaCO3","116 mg CaCO3/L","366 mg/L (nominal = 0 mg/L, but buffer used to adjust pH changed DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-21"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",20,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs (128.7-5715.0))","pH-adjusted each day to pH 6","232 mg/L CaCO3","116 mg CaCO3/L","366 mg/L (nominal = 0 mg/L, but buffer used to adjust pH changed DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-22"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",50,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs (2627-12054)","pH-adjusted each day to pH 6","232 mg/L CaCO3","116 mg CaCO3/L","366 mg/L (nominal = 0 mg/L, but buffer used to adjust pH changed DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-23"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","NOEC",0.05,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","",0.05,"pH-adjusted each day to pH 6","240 mg/L CaCO3","120 mg CaCO3/L","370 mg/L (nominal = 2 mg/L, but buffer used to adjust pH changed DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-24"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","LOEC",0.05,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","",0.05,"pH-adjusted each day to pH 6","240 mg/L CaCO3","120 mg CaCO3/L","370 mg/L (nominal = 2 mg/L, but buffer used to adjust pH changed DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-25"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",10,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs (256-30283)","pH-adjusted each day to pH 6","240 mg/L CaCO3","120 mg CaCO3/L","370 mg/L (nominal = 2 mg/L, but buffer used to adjust pH changed DOC)",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-26"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,93,0.93,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",10,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs","pH-adjusted each day to pH 8 using buffers","10 mg/L CaCO3","228 mg CaCO3/L","<0.5 measured without buffer, but other treatments show buffer added around 350 mg/LK DOC. Nominla DOC 0.",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-28"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",10,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs","pH-adjusted each day to pH 8 using buffers","12 mg/L CaCO3","228 mg CaCO3/L","393 mg/L, due to buffer addition. Nominal = 4 mg/L",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-29"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,1,3,3,3,3,94,0.94,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",10,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs","pH-adjusted each day to pH 8 using buffers","84 mg/L CaCO3","16 (? lower than all rest) mg CaCO3/L","407 mg/L, due to buffer addition. Nominal = 0 mg/L",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-30"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",10,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs","pH-adjusted each day to pH 8 using buffers","79 mg/L CaCO3","196 mg CaCO3/L","455 mg/L, due to buffer addition. Nominal = 4 mg/L",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","187-31"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,3,3,3,3,96,0.96,""]
["Answers","7 d","The fish from each replicate were then placed into a pre-weighed pan and dried at 105°C for over 12 hours and then re-weighed to the nearest 0.01 mg to obtain a dry weight.","EC",10,"Diluent controls","four replicates","USEPA methods, all test acceptability criteria were met.","<24h old","reconstituted lab water, USEPA methods. To various hardnesses.","static renewal. Renewed on days 2,4.","Measured throughout for total and dissolved. Final values expressed as total only.","","Observable from tables","","95% CLs","pH-adjusted each day to pH 8 using buffers","244 mg/L CaCO3","200 mg CaCO3/L","490 mg/L, due to buffer addition. Nominal = 0 mg/L",">/= 60%","25±2°C","Control analysed for metals total and dissolved"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","188-1"]
["Scores",10,5,5,5,5,5,2,5,5,0,0,0,0,4,4,1,0,0,0,0,1,0,57,0.56999999999999995,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Immobilisation","EC",50,"Diluent Controls","Entire test repeated 3 times.","OECD guidelines","<24 h","standard reference water. Recipe given for salts.","Assume static","","","","Regression analyses, OECD","SD",7.6,"","","","","20 ± 2","not stated"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","189-1"]
["Scores",10,5,5,5,5,5,2,5,5,0,0,0,0,4,0,1,0,0,0,0,1,3,56,0.56000000000000005,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Immobilisation","EC",50,"diluent controls","20 animals per treatments. Test repeated three times. ","OECD 1980","<24h old","SRW - recipe given","assume static","","","","Regression analyses and log transformations, GraphPad 4.","",7.6,"","","","","21 ± 1","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","190-1"]
["Scores",10,0,5,5,5,5,2,0,0,0,0,0,0,4,0,1,0,0,0,0,0,3,40,0.4,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Only in Tables does it state LC50, but he measured endpoint is assumed to be mortality.","LC",50,"Not stated but likley","triplicate","OECD guidelines","assume neonate","Previous publication referred to in this article states EPA freshwater medium","","","not stated","","Trimmed spearman Karber.","","5.5, adjusted using Hcl.","","","","","","99.6% purity."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","191-1"]
["Scores",10,5,5,5,5,5,2,5,5,0,4,0,4,4,4,1,0,0,0,1,1,3,69,0.69,""]
["Answers","52 h","Mortality","LC",50,"Artificial pond water controls.  pH control series also tested, as were buffer range controls. No effect due to pH.","triplicate","Control values are provided - 94% survival in pond wateer. N = 11.","6-10 mm males only, collected upstream of iron-polluted site. Measured contaminants at site are low.","artificial pond water. Some constituents are provided in uM concentrations.","Assume static","Measured using phenanthroline technique\n(Johnson, 1955). Fe II measured. For low pH (4.5) test, initial Fe II used as it did not change much over the 52 h.","","Observed in table of results","Probit and regression analyses. Probit data tabulated here only.","95% CLs","Stated as adjusted to pH 4.5 using Hcl. But no mention if adjusted daily or just at test commencement. Also not clear what the pH was in each treatment.","","","","stated as being 'at saturation' due to use of aeration. However aeration also caused Fe III formation.","15 +/- 1","Analar FeSO4.7H2O"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","191-2"]
["Scores",10,5,5,5,5,5,2,5,5,0,4,0,4,4,4,1,0,0,0,1,1,3,69,0.69,""]
["Answers","52 h","Mortality","LC",50,"Artificial pond water","triplicate","Control values are provided - 94% survival in pond wateer. N = 11. pH control series also tested, as were buffer range controls. No effect due to pH.","6-10 mm males only, collected upstream of iron-polluted site. Measured contaminants at site are low.","artificial pond water. Some constituents are provided in uM concentrations.","Assume static","Measured using phenanthroline technique\n(Johnson, 1955). Fe II measured. For buffered pH 6 tests, median concentration between initial and final concentration of Fe(II) as there was a decrease in Fe(II) over the test period.","","Observed in table of results and sicussed in text","Probit and regression analyses. Probit data tabulated here only.","95% CLs","Stated as adjusted using buffers to pH 6. Not clear if adjusted daily or just at test commencement. Also not clear what the pH was in each treatment.","","","","stated as being 'at saturation' due to use of aeration. However aeration also caused Fe III formation.","15 +/- 1","Analar FeSO4.7H2O"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","192-1"]
["Scores",10,10,5,5,5,5,2,5,5,4,0,0,0,4,4,1,1,0,0,0,1,0,67,0.67,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","Considered dead when they failed to respond to physical stimulus","LC",50,"Port Moresby controls","Unclear, but test repeated","Control mortalities discussed. Only 96h data acceptable. Unacceptable mortalities occurred beyond 96h exposure. Therefore up to 9 day data not tabulated.","females, 20-40 mm (mean 28 mm, SD 4 mm)","Port Moresby tap water","Static","","","","Finney et al","95% CLs in appendix tables","Measured in tap water 6.9-7.5. Also measured in tailings concentrations but not reported for metals tests.","40 mg CaCO3/L","","","","22-28",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","192-2"]
["Scores",10,10,5,5,5,5,2,5,5,4,0,0,0,4,4,1,1,0,0,0,1,0,67,0.67,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","Considered dead when they failed to respond to physical stimulus","LC",50,"Port Moresby controls","Unclear, but test repeated","Control mortalities discussed. Only 96h data acceptable. Unacceptable mortalities occurred beyond 96h exposure. Therefore up to 9 day data not tabulated.","females, 20-40 mm (mean 28 mm, SD 4 mm)","Port Moresby tap water","Static","","","","Finney et al","95% CLs in appendix tables","Measured in tap water 6.9-7.5. Also measured in tailings concentrations but not reported for metals tests.","40 mg CaCO3/L","","","","22-28",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","192-3"]
["Scores",10,10,5,5,5,5,2,5,5,4,0,0,0,4,4,1,1,0,0,0,1,0,67,0.67,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","Considered dead when they failed to respond to physical stimulus","LC",50,"Port Moresby controls","Unclear, but test repeated","Control mortalities always <10%","mean 22 mm, SD 3 mm","Port Moresby tap water","Static","","","","Finney et al","95% CLs in appendix tables","Measured in tap water 6.9-7.5. Also measured in tailings concentrations but not reported for metals tests.","40 mg CaCO3/L","","","","22-28",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","193-1"]
["Scores",10,5,5,5,5,5,2,5,5,0,0,4,0,4,0,0,0,0,0,0,1,0,56,0.56000000000000005,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Immobility - not defined.","EC",50,"Diluent control, solvent control (for sample tests)","four replicates","OECD 1984","<24h old females","Tap water filtered through charcoal","","","Fe was a ref tox test, alongside Cu, LAS, PCP, Zn","","Probit in R","","Details given for reproduction tests, but not for acute tests.","Details given for reproduction tests, but not for acute tests.","","Details given for reproduction tests, but not for acute tests.","Details given for reproduction tests, but not for acute tests.","21 ± 1 °C","not stated."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","193-2"]
["Scores",10,5,5,5,5,5,2,5,5,0,0,4,0,4,0,0,0,0,0,0,1,0,56,0.56000000000000005,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Immobility - not defined.","EC",50,"Diluent control, solvent control (for sample tests)","four replicates","OECD 1984","<24h old females","Tap water filtered through charcoal","","","Fe was a ref tox test, alongside Cu, LAS, PCP, Zn","","Probit in R","","Details given for reproduction tests, but not for acute tests.","Details given for reproduction tests, but not for acute tests.","","Details given for reproduction tests, but not for acute tests.","Details given for reproduction tests, but not for acute tests.","21 ± 1 °C","not stated."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","193-3"]
["Scores",10,5,5,5,5,5,2,5,5,0,0,4,0,4,0,0,0,0,0,0,1,0,56,0.56000000000000005,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Immobility - not defined.","EC",50,"Diluent control, solvent control (for sample tests)","four replicates","OECD 1984","<24h old females","Tap water filtered through charcoal","","","Fe was a ref tox test, alongside Cu, LAS, PCP, Zn","","Probit in R","","Details given for reproduction tests, but not for acute tests.","Details given for reproduction tests, but not for acute tests.","","Details given for reproduction tests, but not for acute tests.","Details given for reproduction tests, but not for acute tests.","21 ± 1 °C","not stated."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","194-1"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,4,3,1,1,0,1,1,3,72,0.72,""]
["Answers","96 h","Mortality","LC",50,"Diluent controls","","OECD 203","Age: 4 months, Length: 2.9 ± 0.1 cm, Weight: 0.22 ± 0.03 g, ","tap water passed through activated carbon filter, phys-chem provided","static","total Fe","not stated","In tables","Probit method (USEPA/600/4-85/13)","95% CLs of 36100-61500","Day 0 5.7 (100 mg/L) - 7.42 (Control). By 96h, measured concs of 34400 and 62600 pH 3.5-4.1. Rest was >6.8.","34 mg/L CACO3/L","24 mg/L CaCO3/L","","65-97%","23.7 ± 0.3","98% purity, FeCl2"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","195-1"]
["Scores",10,5,5,5,5,5,2,0,5,0,0,0,4,4,4,1,1,1,0,1,1,3,62,0.62,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Mortality","LC",50,"Diluent controls","5 reps","APHA methods","","24-h aged borehole water, physico-chem provided.","Assume static","","","Conc response was normal until 15 mg/L, when 52% effect observed. At concs of 30, 100, 125, 150, 175, 200 mg/L, mortality was 52, 58, 54, 76, 56%. Therefore, LC50 of 86 may not be accurate. Cls will go from 15 to 200 mg/L.","Regression analyses used.","95% Cls 55500-117750","7 +/- 0.5","280 mg/L CaCO3","250 mg/L CaCO3","","9 mg/L","24-26","reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","195-2"]
["Scores",10,5,5,5,5,5,2,0,5,0,0,0,4,4,4,1,1,1,0,1,1,3,62,0.62,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Mortality","LC",5,"Diluent controls","5 reps","APHA methods","","24-h aged borehole water, physico-chem provided.","Assume static","","","Conc response was normal until 15 mg/L, when 52% effect observed. At concs of 30, 100, 125, 150, 175, 200 mg/L, mortality was 52, 58, 54, 76, 56%. Therefore, LC50 of 86 may not be accurate. Cls will go from 15 to 200 mg/L.","Regression analyses used.","95% Cls 55500-117750","7 +/- 0.5","280 mg/L CaCO3","250 mg/L CaCO3","","9 mg/L","24-26","reagent grade. Ferrous sulfate"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-1"]
["Scores",10,0,5,5,5,5,2,5,5,0,0,0,0,0,0,1,1,1,0,1,1,3,50,0.5,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",50,"Diluent controls","5 replicates","APHA 1975","69.9 +/- 0.57 mm, 6.02 +/- 0.133 g.","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",7,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-2"]
["Scores",10,0,5,5,5,5,2,5,5,0,0,0,0,0,0,1,1,1,0,1,1,3,50,0.5,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",50,"Diluent controls","5 replicates","APHA 1975","69.9 +/- 0.57 mm, 6.02 +/- 0.133 g.","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",7,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-3"]
["Scores",10,0,5,5,5,5,2,5,5,0,0,0,0,0,0,1,1,1,0,1,1,3,50,0.5,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",50,"Diluent controls","5 replicates","APHA 1975","69.9 +/- 0.57 mm, 6.02 +/- 0.133 g.","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",6.5,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-4"]
["Scores",10,0,5,5,5,5,2,5,5,0,0,0,0,0,0,1,1,1,0,1,1,3,50,0.5,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",5,"Diluent controls","5 replicates","APHA 1975","69.9 +/- 0.57 mm, 6.02 +/- 0.133 g.","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",6.5,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-5"]
["Scores",10,0,5,5,5,5,2,5,5,0,0,0,0,0,0,1,1,1,0,1,1,3,50,0.5,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",50,"Diluent controls","5 replicates","APHA 1975","69.9 +/- 0.57 mm, 6.02 +/- 0.133 g.","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",8.5,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-6"]
["Scores",10,0,5,5,5,5,2,5,5,0,0,0,0,0,0,1,1,1,0,1,1,3,50,0.5,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",5,"Diluent controls","5 replicates","APHA 1975","69.9 +/- 0.57 mm, 6.02 +/- 0.133 g.","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",8.5,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-7"]
["Scores",10,0,5,5,5,5,2,0,5,0,0,0,0,0,0,1,1,1,0,1,1,3,45,0.45,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",50,"Diluent controls","5 replicates","APHA 1975","","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",7,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-8"]
["Scores",10,0,5,5,5,5,2,0,5,0,0,0,0,0,0,1,1,1,0,1,1,3,45,0.45,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",5,"Diluent controls","5 replicates","APHA 1975","","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",7,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-9"]
["Scores",10,0,5,5,5,5,2,0,5,0,0,0,0,0,0,1,1,1,0,1,1,3,45,0.45,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",50,"Diluent controls","5 replicates","APHA 1975","","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",6.5,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-10"]
["Scores",10,0,5,5,5,5,2,0,5,0,0,0,0,0,0,1,1,1,0,1,1,3,45,0.45,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",5,"Diluent controls","5 replicates","APHA 1975","","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",6.5,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-11"]
["Scores",10,0,5,5,5,5,2,0,5,0,0,0,0,0,0,1,1,1,0,1,1,3,45,0.45,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",50,"Diluent controls","5 replicates","APHA 1975","","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",6.5,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-12"]
["Scores",10,0,5,5,5,5,2,0,5,0,0,0,0,0,0,1,1,1,0,1,1,3,45,0.45,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",5,"Diluent controls","5 replicates","APHA 1975","","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",6.5,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-13"]
["Scores",10,0,5,5,5,5,2,0,5,0,0,0,0,0,0,1,1,1,0,1,1,3,45,0.45,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",50,"Diluent controls","5 replicates","APHA 1975","","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",7,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-14"]
["Scores",10,0,5,5,5,5,2,0,5,0,0,0,0,0,0,1,1,1,0,1,1,3,45,0.45,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",5,"Diluent controls","5 replicates","APHA 1975","","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",7,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-15"]
["Scores",10,0,5,5,5,5,2,0,5,0,0,0,0,0,0,1,1,1,0,1,1,3,45,0.45,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",50,"Diluent controls","5 replicates","APHA 1975","","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",6.5,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-16"]
["Scores",10,0,5,5,5,5,2,0,5,0,0,0,0,0,0,1,1,1,0,1,1,3,45,0.45,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",5,"Diluent controls","5 replicates","APHA 1975","","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",6.5,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-17"]
["Scores",10,0,5,5,5,5,2,0,5,0,0,0,0,0,0,1,1,1,0,1,1,3,45,0.45,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",50,"Diluent controls","5 replicates","APHA 1975","","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",8.5,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","196-18"]
["Scores",10,0,5,5,5,5,2,0,5,0,0,0,0,0,0,1,1,1,0,1,1,3,45,0.45,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","","LC",5,"Diluent controls","5 replicates","APHA 1975","","Unchlorinated borehole water, phys-chem provided.","","","","","Not stated.","",8.5,"268 mg/L CaCO3","115 mg/L","","7.5 mg/L","27 +/- 2","Reagent grade."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","197-1"]
["Scores",10,10,5,5,5,5,2,5,5,0,0,0,0,4,4,3,3,3,0,3,3,0,75,0.75,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Death was determined by complete immobilisation and no heart beat and also no response to gently prodding with a blunt glass rod.","LC",50,"Diluent controls","duplicates","APHA 1977","12h old","filtered aerated tubewell water, characterised in table1.","","","","","moving angle average Harris 1990","95% CLs  20990-32350","7.6 (7.4-7.8) in test waters water","240 mg/L in test waters(235-260)","400 mg/L in test waters (390-415)","","5.6 mg/L in test waters 5.2-6.5","13 (11.5-14.5)","ferric chloride"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","198-1"]
["Scores",10,0,5,5,5,5,0,0,5,4,4,0,0,0,0,1,1,0,0,1,1,0,47,0.47,""]
["Answers","96 h","Assume mortality","LC",50,"Diluent controls","3 replicates","However - assume this to be high quality work, soince it was done for GV in British Columbia","","Well water, hardness 100 mg/L","Static","Measured beginning and end. However, not clear how the measured values were used. Initial values used? Mean? Values are not reported either. ","not stated","not stated","not stated","","minimum pH of 5 maintained. Measured in all test solutions beginning and end but not reported.","50 mg/L","","","Measured throughout but not stated.","15 °C","No specific results or statements around controls being measured for Fe. Nor grade of chemcial used. Ferric chloride"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","198-2"]
["Scores",10,0,5,5,5,5,0,0,5,4,4,0,0,0,0,1,1,0,0,1,1,0,47,0.47,""]
["Answers","96 h","Assume mortality","LC",50,"Diluent controls","3 replicates","However - assume this to be high quality work, soince it was done for GV in British Columbia","","Softwater hardness 50 mg/L","Static","Measured beginning and end. However, not clear how the measured values were used. Initial values used? Mean? Values are not reported either. ","not stated","not stated","not stated","","minimum pH of 5 maintained. Measured in all test solutions beginning and end but not reported.","50 mg/L","","","Measured throughout but not stated.","23 °C","No specific results or statements around controls being measured for Fe. Nor grade of chemcial used. Ferric chloride"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","198-3"]
["Scores",10,0,5,5,5,5,0,0,5,4,4,0,0,0,0,1,1,0,0,1,1,0,47,0.47,""]
["Answers","21 d","Assume reproduction","NOEC","","Diluent controls","10 replicates","However - assume this to be high quality work, soince it was done for GV in British Columbia","","Wellwater, hardness 100 mg/L","Static","Measured beginning and end. However, not clear how the measured values were used. Initial values used? Mean? Values are not reported either. ","not stated","not stated","not stated","","minimum pH of 5 maintained. Measured in all test solutions beginning and end but not reported.","100 mg/L","","","Measured throughout but not stated.","20 °C","No specific results or statements around controls being measured for Fe. Nor grade of chemcial used. Ferric chloride"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","199-1"]
["Scores",10,5,0,5,5,5,0,5,5,0,4,0,4,0,0,1,0,0,0,0,1,3,53,0.53,""]
["Answers","96 h","Growth - length measured by ocular micrometer","","NOEC-equivalent data obtained from graph and description of results.","Diluent controls","replicates within test not defined but variability on graphs shows replicates were used. Test repeated 5 times with different waters.","","First instar larvae, <24h old","Lake Maarsseveen water and several river waters.","","Beginning and end, but not stated how the data used or what the final measured concs reflect (i.e. mean?).","","","Unclear","","Controls 7.8, and top two concentrations adjusted to 7.8.","","","","","20 ± 1 °C","<0.001µM Fe in Controls. FeCl3 used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","200-1"]
["Scores",10,5,0,5,0,0,0,0,0,0,0,0,4,0,4,1,0,0,0,0,0,0,29,0.28999999999999998,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","","","NOEC can be inferred from text and data","Unclear - not stated","Not stated","","","But culture water was described, containing 350 mg/L MgSO4, 540 mg/L NaCHO3, 10 mg/L KCl, 210 mg/L CaSO4","","","However, inert China clay tested alongside as a control for precipitate to comare with Fe precipitate","","Not described",0.05,"In discussion stated that pHs were 7-8.","But will be high based on the ingredients in the culture water. 350 mg/L MgSO4, 540 mg/L NaHCO3, 10 mg/L KCl, 210 mg/L CaSO4","","","","",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","200-2"]
["Scores",10,5,5,5,0,0,0,0,0,0,0,0,4,4,4,1,0,0,0,0,0,0,38,0.38,"The highest possible grade for this datum is acceptable."]
["Answers","48 h","Mortality","ED50 (although as described, I think this is EC50)",50,"Unclear - not stated","Not stated","","","But culture water was described, containing 350 mg/L MgSO4, 540 mg/L NaCHO3, 10 mg/L KCl, 210 mg/L CaSO4","","","However, inert China clay tested alongside as a control for precipitate to comare with Fe precipitate","","Litchfield and Wolcoxen","95% CLs 12.39-10.63 mg/L","In discussion stated that pHs were 7-8.","But will be high based on the ingredients in the culture water. 350 mg/L MgSO4, 540 mg/L NaHCO3, 10 mg/L KCl, 210 mg/L CaSO4","","","","",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","200-3"]
["Scores",10,5,0,5,0,0,0,0,0,0,0,0,4,4,4,1,0,0,0,0,0,0,33,0.33,"The highest possible grade for this datum is acceptable."]
["Answers","21 d","Mortality","","NOEC described in text (as where significant p<0.05) effects occurred)","Unclear - not stated","Not stated","","","But culture water was described, containing 350 mg/L MgSO4, 540 mg/L NaCHO3, 10 mg/L KCl, 210 mg/L CaSO4","","","However, inert China clay tested alongside as a control for precipitate to comare with Fe precipitate","","Litchfield and Wolcoxen",0.05,"In discussion stated that pHs were 7-8.","But will be high based on the ingredients in the culture water. 350 mg/L MgSO4, 540 mg/L NaHCO3, 10 mg/L KCl, 210 mg/L CaSO4","","","","",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","200-4"]
["Scores",10,5,5,5,0,0,0,0,0,0,0,0,4,4,4,1,0,0,0,0,0,0,38,0.38,"The highest possible grade for this datum is acceptable."]
["Answers","21 d","number of broods and mean cluthc size. Although not clear which is the endpoint for the described ED50","ED50 (althgou from description of method I think it is EC50)",50,"Unclear - not stated","Not stated","","","But culture water was described, containing 350 mg/L MgSO4, 540 mg/L NaCHO3, 10 mg/L KCl, 210 mg/L CaSO4","","","However, inert China clay tested alongside as a control for precipitate to comare with Fe precipitate","","Litchfield and Wolcoxen","90% CLs 3090-6510 µg/L","In discussion stated that pHs were 7-8.","But will be high based on the ingredients in the culture water. 350 mg/L MgSO4, 540 mg/L NaHCO3, 10 mg/L KCl, 210 mg/L CaSO4","","","","",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","201-1"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,0,4,3,3,3,0,3,1,3,78,0.78,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","The criterion for determining death was lack of movement and failure to respond to a gentle probing with a blunt dissecting needle.","LC",50,"non-toxicant controls","duplicate","Controls with mortalitygreater than 10% were excluded from the calculation and it was repeated. Physicochemical properties of the test water were analyzed using standard procedures (APHA et al., 1989). ","","Ground well water. Physico-chemical parameters measured and provided","Static, non renewal.","","But 10 heavy meatls tested altogether.","Observed","Probably, since they followed APHA, but no description of method","95% CLs 73560-103790","7.5 (range 7.3-7.8)","237 (range 226-255) mg CaCO3/L","380 (range 360-415) mg/L CaCO3","","5.7 mg/L (range 5.1-6.5)","15°C in temperature controlled cabinet","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","201-2"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,0,4,3,3,3,0,3,1,3,78,0.78,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","The criterion for determining death was lack of movement and failure to respond to a gentle probing with a blunt dissecting needle.","LC",50,"non-toxicant controls","duplicate","Controls with mortalitygreater than 10% were excluded from the calculation and it was repeated. Physicochemical properties of the test water were analyzed using standard procedures (APHA et al., 1989). ","","Ground well water. Physico-chemical parameters measured and provided","Static, non renewal.","","But 10 heavy meatls tested altogether.","Observed","Probably, since they followed APHA, but no description of method","95% CLs 106050-147990","7.5 (range 7.3-7.8)","237 (range 226-255) mg CaCO3/L","380 (range 360-415) mg/L CaCO3","","5.7 mg/L (range 5.1-6.5)","15°C in temperature controlled cabinet","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","201-3"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,0,4,3,3,3,0,3,1,3,78,0.78,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","The criterion for determining death was lack of movement and failure to respond to a gentle probing with a blunt dissecting needle.","LC",50,"non-toxicant controls","duplicate","Controls with mortalitygreater than 10% were excluded from the calculation and it was repeated. Physicochemical properties of the test water were analyzed using standard procedures (APHA et al., 1989). ","","Ground well water. Physico-chemical parameters measured and provided","Static, non renewal.","","But 10 heavy meatls tested altogether.","Observed","Probably, since they followed APHA, but no description of method","95% CLs 80110-121230","7.5 (range 7.3-7.8)","237 (range 226-255) mg CaCO3/L","380 (range 360-415) mg/L CaCO3","","5.7 mg/L (range 5.1-6.5)","15°C in temperature controlled cabinet","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","201-4"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,0,4,3,3,3,0,3,1,3,78,0.78,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","The criterion for determining death was lack of movement and failure to respond to a gentle probing with a blunt dissecting needle.","LC",50,"non-toxicant controls","duplicate","Controls with mortalitygreater than 10% were excluded from the calculation and it was repeated. Physicochemical properties of the test water were analyzed using standard procedures (APHA et al., 1989). ","","Ground well water. Physico-chemical parameters measured and provided","Static, non renewal.","","But 10 heavy meatls tested altogether.","Observed","Probably, since they followed APHA, but no description of method","95% CLs 58700-90540","7.5 (range 7.3-7.8)","237 (range 226-255) mg CaCO3/L","380 (range 360-415) mg/L CaCO3","","5.7 mg/L (range 5.1-6.5)","15°C in temperature controlled cabinet","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","202-1"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,0,3,3,3,0,3,3,3,86,0.86,""]
["Answers","96 h","Death was defined as the absence of all visible signs of mobility, including the  antennae, thoracic appendages, and the postabdomen during a five-second observation after gentle prodding with a glass pipet.","LC",50,"Non-toxicant controls","duplicate","ASTM methods, also 100% survival in controls.","0.912 ± 0.081 g","ASTM reconstituted water","Static renewal, 12-h renewals.","Measured throughout test.","","Observable","ANOVA for NOEC/LOEC, probit for LC50 values","95% CLs could not be determined for this test","6.34 ±\u0090 1.5","100.3 ± 8.1","24.1 ± 17.7","","8.1 ± 0.1","21.5 ± 0.3","Reagent grade FeCl3.6H2O, all test concentrations measured including controls"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","202-2"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,90,0.9,""]
["Answers","96 h","Death was defined as the absence of all visible signs of mobility, including the  antennae, thoracic appendages, and the postabdomen during a five-second observation after gentle prodding with a glass pipet.","LC",50,"Non-toxicant controls","duplicate","ASTM methods, also 100% survival in controls.","0.15 ± 0.005 g","ASTM reconstituted water","Static renewal, 12-h renewals.","Measured throughout test.","","Observable","ANOVA for NOEC/LOEC, probit for LC50 values","95% CLs calculated but may not be reliable due to all-or-nothing response of fish","6.66 ± 1.11","99.6 ± 7.9","30.2 ± 18.4","","8.2 ± 0.1","21.6 ± 0.5","Reagent grade FeCl3.6H2O, all test concentrations measured including controls"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","202-3"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,90,0.9,""]
["Answers","96 h","Death was defined as the absence of all visible signs of mobility, including the  antennae, thoracic appendages, and the postabdomen during a five-second observation after gentle prodding with a glass pipet.","LC",50,"Non-toxicant controls","duplicate","ASTM methods, also 100% survival in controls.","0.008 ± 0.003 g","ASTM reconstituted water","Static renewal, 12-h renewals.","Measured throughout test.","","Observable","ANOVA for NOEC/LOEC, probit for LC50 values","95% CLs  52050-197890","4.92 ± 2.03","115.5 ± 27.4","22.5 ± 18.2","","8.2 ± 0.1","20.9 ± 0.2","Reagent grade FeCl3.6H2O, all test concentrations measured including controls"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","202-4"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,90,0.9,""]
["Answers","96 h","Death was defined as the absence of all visible signs of mobility, including the  antennae, thoracic appendages, and the postabdomen during a five-second observation after gentle prodding with a glass pipet.","LC",50,"Non-toxicant controls","duplicate","ASTM methods, also 100% survival in controls.","0.236 ± 0.003 g","ASTM reconstituted water","Static renewal, 12-h renewals.","Measured throughout test.","","Observable","ANOVA for NOEC/LOEC, probit for LC50 values","95% CLs  10410-13900","4.92 ± 2.03","115.5 ± 27.4","22.5 ± 18.2","","8.2 ± 0.1","20.9 ± 0.2","Reagent grade FeCl3.6H2O, all test concentrations measured including controls"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","202-5"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,90,0.9,""]
["Answers","96 h","Death was defined as the absence of all visible signs of mobility, including the  antennae, thoracic appendages, and the postabdomen during a five-second observation after gentle prodding with a glass pipet.","LC",50,"Non-toxicant controls","duplicate","ASTM methods, also 100% survival in controls.","First instar stage","ASTM reconstituted water","Data combined for static and staic-renewal tests, each with 2 replicates","Measured throughout test.","","Observable","ANOVA for NOEC/LOEC, probit for LC50 values","95% CLs  10410-13900","6.06 ± 1.41","96.2 ± 5.3","27.9 ± 19.1","","8.8 ± 0.2","20.3 ± 0.2","Reagent grade FeCl3.6H2O, all test concentrations measured including controls"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","202-6"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,90,0.9,""]
["Answers","21 d","Reproduction data: brood size","NOEC","p < 0.05","Non-toxicant controls","duplicate","ASTM methods, also 100% survival in controls.","neonates","ASTM reconstituted water","Data combined for static and staic-renewal tests, each with 2 replicates","Measured throughout test.","","Observable","ANOVA for NOEC/LOEC, probit for LC50 values","0.05 level or less","7.57 ± 0.22","93.6 ± 12.7","47.5 ± 6.3","","9.0 ± 0.2","20.1 ± 0.1","Reagent grade FeCl3.6H2O, all test concentrations measured including controls"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","202-7"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,90,0.9,""]
["Answers","96 h","Death was defined as the absence of all visible signs of mobility, including the  antennae, thoracic appendages, and the postabdomen during a five-second observation after gentle prodding with a glass pipet.","LC",50,"Non-toxicant controls","duplicate","ASTM methods, also 100% survival in controls.","First Instar stage","ASTM reconstituted water","Static non-renewal","Measured throughout test.","","Observable","ANOVA for NOEC/LOEC, probit for LC50 values","95% Cls","6.41 ± 1.4","87.8 ± 9.8","13.6 ± 15","","9.0 ± 0.2","20.0 ± 0.07","Reagent grade FeCl3.6H2O, all test concentrations measured including controls"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","202-8"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,90,0.9,""]
["Answers","96 h","Death was defined as the absence of all visible signs of mobility, including the  antennae, thoracic appendages, and the postabdomen during a five-second observation after gentle prodding with a glass pipet.","LC",50,"Non-toxicant controls","duplicate","ASTM methods, also 100% survival in controls.","First Instar stage","ASTM reconstituted water","Static non-renewal","Measured throughout test.","","Observable","ANOVA for NOEC/LOEC, probit for LC50 values","95% Cls","5.91 ± 1.74","90.4 ± 14.8","16.7 ± 19.3","","8.5 ± 0.3","20.0 ± 0.03","Reagent grade FeCl3.6H2O, all test concentrations measured including controls"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","202-9"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,90,0.9,""]
["Answers","96 h","Death was defined as the absence of all visible signs of mobility, including the  antennae, thoracic appendages, and the postabdomen during a five-second observation after gentle prodding with a glass pipet.","LC",50,"Non-toxicant controls","duplicate","ASTM methods, also 100% survival in controls.","First Instar stage","ASTM reconstituted water","Static non-renewal","Measured throughout test.","","Observable","ANOVA for NOEC/LOEC, probit for LC50 values","95% Cls","6.10 ± 1.78","83.1 ± 4.7","12.6 ± 14.6","","8.2 ± 0.1","20.0 ± 0.00","Reagent grade FeCl3.6H2O, all test concentrations measured including controls"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","202-10"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,85,0.85,""]
["Answers","33 d"," In fish early life stage studies, test responses included growth, mortality, and  teratogenesis.","NOEC","p<0.05","Non-toxicant controls","duplicate","ASTM methods, also 100% survival in controls.","First Instar stage","ASTM reconstituted water","Static renewal, 12-h","Measured at 3-d intervals, with water samples collected within the first h after solutions were renwed.","","Observable","ANOVA for NOEC/LOEC, probit for LC50 values","p<0.05","7.57 ± 0.22","93.6 ± 12.7","47.5 ± 6.3","","9.0 ± 0.2","20.1 ± 0.1","Reagent grade FeCl3.6H2O, all test concentrations measured including controls"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","203-1"]
["Scores",10,10,0,0,5,5,2,5,5,4,4,0,4,4,4,3,1,1,0,3,1,0,71,0.71,""]
["Answers","Exposure not explicit in the method but used to describe effects in results","defined as dead when no tactile response after 2 h recovery in control water","Did ANOVA testing and Tukey's to statistically test for differences","No effect concentration","","3 replicates","Use of ASTM protocol","third instar used","reconstituted soft water (adjusted to pH 4)","static renewal daily","Measured total and dissolved (<0.45 µm filtered) Fe in stocks NOT in test solutions at beginning and end","","","ANOVA for NOEC","","Measured in test beakers","Was measured but not stated","Was measured but not stated","","DO >60% saturation in test beakers","Stated as 22oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","203-2"]
["Scores",10,10,0,0,5,5,2,5,5,4,4,0,4,4,4,3,1,1,0,3,1,0,71,0.71,""]
["Answers","Exposure not explicit in the method but used to describe effects in results","defined as dead when no tactile response after 2 h recovery in control water","Did ANOVA testing and Tukey's to statistically test for differences","No effect concentration","","3 replicates","Use of ASTM protocol","Adult Male","reconstituted soft water (adjusted to pH 4)","static renewal daily","Measured total and dissolved (<0.45 µm filtered) Fe in stocks NOT in test solutions at beginning and end","","","ANOVA for NOEC","","Measured in test beakers","Was measured but not stated","Was measured but not stated","","DO >60% saturation in test beakers","Stated as 22oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","204-1"]
["Scores",10,5,5,5,5,5,0,5,5,0,0,0,0,4,4,0,0,0,0,0,1,3,57,0.56999999999999995,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Dead as defined under stereomicroscope","NOEC","","","5 reps","","< 24 h neonates","USEPA media pH 7.5","24 h no water replacement","","","","probit analysis","95 % CL","only stated for the test media not the test solutions","","","","","25 oC","Used AA standards preserved in 2 % nitric acid - no mention of pH effects"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","204-2"]
["Scores",10,5,5,5,5,5,0,5,5,0,0,0,0,4,4,0,0,0,0,0,1,3,57,0.56999999999999995,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Dead as defined under stereomicroscope","LC50","","","5 reps","","< 24 h neonates","USEPA media pH 7.5","24 h no water replacement","","","","probit analysis","95 % CL","only stated for the test media not the test solutions","","","","","25 oC","Used AA standards preserved in 2 % nitric acid - no mention of pH effects"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","204-3"]
["Scores",10,5,5,5,5,5,0,5,5,0,0,0,0,4,4,0,0,0,0,0,1,3,57,0.56999999999999995,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Dead as defined under stereomicroscope","NOEC","","","5 reps","","< 24 h neonates","USEPA media pH 7.5","24 h no water replacement","","","","probit analysis","95 % CL","only stated for the test media not the test solutions","","","","","25 oC","Used AA standards preserved in 2 % nitric acid - no mention of pH effects"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","204-4"]
["Scores",10,5,5,5,5,5,0,5,5,0,0,0,0,4,4,0,0,0,0,0,1,3,57,0.56999999999999995,"The highest possible grade for this datum is acceptable."]
["Answers","24 h","Dead as defined under stereomicroscope","LC50","","","5 reps","","< 24 h neonates","USEPA media pH 7.5","24 h no water replacement","","","","probit analysis","95 % CL","only stated for the test media not the test solutions","","","","","25 oC","Used AA standards preserved in 2 % nitric acid - no mention of pH effects"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","205-1"]
["Scores",10,10,5,5,5,0,0,5,5,0,0,0,0,4,0,0,0,0,0,0,0,3,52,0.52,"The highest possible grade for this datum is acceptable."]
["Answers","9-h in flask experiment","Relative doubling time as a percentage of the control","IC50","50% effect","","Replication of test flasks not mentioned but the experiment was replicated 3-5 times","","exponential phase","test solutions made in deionized water","Assume static","nominal","","","linear regression analysis of exponential phase of growth","","","","","","","","Purity stated as 99% or greater"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","206-1"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,0,0,3,1,3,85,0.85,""]
["Answers","24 h","death determined by lack of movement with gentle probing","LC50",0.5,"","5 reps","APHA"," 1 cm body length,  wet weight (0.1-0.3 mg), adult","dechlorinated tap water","Static renewal of solutions every 48 h","Measured before and after each water renewal","But multiple metals were tested","","Use of logarithmic probit paper ","246 - 429 ug/L 95 % CL","6.5 +/-0.01","17.89 mg/L","","","6.25 mg/L","28-30 oC","Analytical grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","206-2"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,0,0,3,1,3,85,0.85,""]
["Answers","48 h","death determined by lack of movement with gentle probing","LC50",0.5,"","5 reps","APHA"," 1 cm body length,  wet weight (0.1-0.3 mg), adult","dechlorinated tap water","Static renewal of solutions every 48 h","Measured before and after each water renewal","But multiple metals were tested","","Use of logarithmic probit paper ","162-312 ug/L 95 % CL","6.5 +/-0.01","17.89 mg/L","","","6.25 mg/L","28-30 oC","Analytical grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","206-3"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,0,0,3,1,3,85,0.85,""]
["Answers","72 h","death determined by lack of movement with gentle probing","LC50",0.5,"","5 reps","APHA"," 1 cm body length,  wet weight (0.1-0.3 mg), adult","dechlorinated tap water","Static renewal of solutions every 48 h","Measured before and after each water renewal","But multiple metals were tested","","Use of logarithmic probit paper ","106 - 237 ug/L 95 % CL","6.5 +/-0.01","17.89 mg/L","","","6.25 mg/L","28-30 oC","Analytical grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","206-4"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,0,0,3,1,3,85,0.85,""]
["Answers","96 h","death determined by lack of movement with gentle probing","LC50",0.5,"","5 reps","APHA"," 1 cm body length,  wet weight (0.1-0.3 mg), adult","dechlorinated tap water","Static renewal of solutions every 48 h","Measured before and after each water renewal","But multiple metals were tested","","Use of logarithmic probit paper ","57 - 174 ug/L 95 % CL","6.5 +/-0.01","17.89 mg/L","","","6.25 mg/L","28-30 oC","Analytical grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","207-1"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,0,0,3,1,0,82,0.82,""]
["Answers","24 h","Defined as dead upon failure to respond to gentle physical stimulation","LC50 also calculated LT50s but I have not reported those",0.5,"","5 reps","Cited APHA","Adult (1.5 mm body length), (0.3 mg wet weight)","dechlorinated tap water","static renewal every 48 h","Measured at beginning and end of water change","","Stated ","Litchfield and Wilcoxon references in FORTRAN","654 - 1200 µg/L 95% CL",6.5,"15.63 mg/L as CaCO3","","","6.25 mg/L","28-30 oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","207-2"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,0,0,3,1,0,82,0.82,""]
["Answers","48 h","Defined as dead upon failure to respond to gentle physical stimulation","LC50 also calculated LT50s but I have not reported those",0.5,"","5 reps","Cited APHA","Adult (1.5 mm body length), (0.3 mg wet weight)","dechlorinated tap water","static renewal every 48 h","Measured at beginning and end of water change","","Stated ","Litchfield and Wilcoxon references in FORTRAN","450 - 770 µg/L 95% CL",6.5,"15.63 mg/L as CaCO3","","","6.25 mg/L","28-30 oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","207-3"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,0,0,3,1,0,82,0.82,""]
["Answers","72 h","Defined as dead upon failure to respond to gentle physical stimulation","LC50 also calculated LT50s but I have not reported those",0.5,"","5 reps","Cited APHA","Adult (1.5 mm body length), (0.3 mg wet weight)","dechlorinated tap water","static renewal every 48 h","Measured at beginning and end of water change","","Stated ","Litchfield and Wilcoxon references in FORTRAN","300 - 617 µg/L 95% CL",6.5,"15.63 mg/L as CaCO3","","","6.25 mg/L","28-30 oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","207-4"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,0,3,3,0,0,3,1,0,78,0.78,""]
["Answers","72 h","Defined as dead upon failure to respond to gentle physical stimulation","LC50 also calculated LT50s but I have not reported those",0.5,"","5 reps","Cited APHA","Adult (1.5 mm body length), (0.3 mg wet weight)","dechlorinated tap water","static renewal every 48 h","Measured at beginning and end of water change","","Stated ","Litchfield and Wilcoxon references in FORTRAN"," 95% CL could not be calculated",6.5,"15.63 mg/L as CaCO3","","","6.25 mg/L","28-30 oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","208-1"]
["Scores",10,10,5,5,5,5,2,5,5,4,2,0,0,4,4,3,3,0,0,3,1,3,79,0.79,""]
["Answers","24-h","Mortality - no response to physical stimulus","LC50",0.5,"","2 reps","APHA ","stage 25 tadpole (wet weight = 0.11 g, body length = 2 - 2.5 mm)","dechlorinated tap water","static renewal every 48 h","measured at beginning of 24 h","","","Litchfield and Wilcoxon in FORTRAN","650 - 1620 µg/L 95% CL",6.5,"18.6 mg/L as CaCO3","","","6.3 mg/L","28 - 30oC","analytical grade chemicals"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","208-2"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,0,4,4,3,3,0,0,3,1,3,81,0.81,""]
["Answers","48-h","Mortality - no response to physical stimulus","LC50",0.5,"","2 reps","APHA ","stage 25 tadpole (wet weight = 0.11 g, body length = 2 - 2.5 mm)","dechlorinated tap water","static renewal every 48 h","measured at beginning and end of 48 h","","","Litchfield and Wilcoxon in FORTRAN","180 - 970 µg/L 95% CL",6.5,"18.6 mg/L as CaCO3","","","6.3 mg/L","28 - 30oC","analytical grade chemicals"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","208-3"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,0,4,4,3,3,0,0,3,1,3,81,0.81,""]
["Answers","72-h","Mortality - no response to physical stimulus","LC50","","","2 reps","APHA ","stage 25 tadpole (wet weight = 0.11 g, body length = 2 - 2.5 mm)","dechlorinated tap water","static renewal every 48 h","measured at beginning and end of 48 h","","","Litchfield and Wilcoxon in FORTRAN","380 - 720 µg/L 95% CL",6.5,"18.6 mg/L as CaCO3","","","6.3 mg/L","28 - 30oC","analytical grade chemicals"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","208-4"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,0,4,0,3,3,0,0,3,1,3,77,0.77,""]
["Answers","96-h","Mortality - no response to physical stimulus","LC50","","","2 reps","APHA ","stage 25 tadpole (wet weight = 0.11 g, body length = 2 - 2.5 mm)","dechlorinated tap water","static renewal every 48 h","measured at beginning and end of 48 h","","","Litchfield and Wilcoxon in FORTRAN"," 95% CL could not be calculated",6.5,"18.6 mg/L as CaCO3","","","6.3 mg/L","28 - 30oC","analytical grade chemicals"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","209-1"]
["Scores",10,10,5,5,5,5,0,5,5,4,2,0,0,4,4,3,3,0,0,3,1,0,74,0.74,""]
["Answers","24-h","Mortality - defined as no response to physical disturbance","LC50","","","5 reps","","4th instar larvae","dechlorinated tap water","Static renewal ","total Fe","","","Litchfield Wilcoxon probit analysis","1640 - 4950 µg/L 95% CL",6.5,"15.6 mg/L as CaCO3","","","6.25 mg/L","28 - 30 oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","209-2"]
["Scores",10,10,5,5,5,5,0,5,5,4,4,0,0,4,4,3,3,0,0,3,1,0,76,0.76,""]
["Answers","48-h","Mortality - defined as no response to physical disturbance","LC50","","","5 reps","","4th instar larvae","dechlorinated tap water","Static renewal ","total Fe measured at beginning at end","","","Litchfield Wilcoxon probit analysis","560 - 2680 µg/L 95% CL",6.5,"15.6 mg/L as CaCO3","","","6.25 mg/L","28 - 30 oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","209-3"]
["Scores",10,10,5,5,5,5,0,5,5,4,2,0,0,4,4,3,3,0,0,3,1,0,74,0.74,""]
["Answers","72-h","Mortality - defined as no response to physical disturbance","LC50","","","5 reps","","4th instar larvae","dechlorinated tap water","Static renewal ","total Fe measured at beginning","","","Litchfield Wilcoxon probit analysis","50 - 1660 µg/L 95% CL",6.5,"15.6 mg/L as CaCO3","","","6.25 mg/L","28 - 30 oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","209-4"]
["Scores",10,10,5,5,5,5,0,5,5,4,4,0,0,4,4,3,3,0,0,3,1,0,76,0.76,""]
["Answers","96-h","Mortality - defined as no response to physical disturbance","LC50","","","5 reps","","4th instar larvae","dechlorinated tap water","Static renewal ","total Fe measured at beginning and end","","","Litchfield Wilcoxon probit analysis","4 - 1250 µg/L 95% CL",6.5,"15.6 mg/L as CaCO3","","","6.25 mg/L","28 - 30 oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","210-1"]
["Scores",10,10,5,5,5,5,2,5,5,4,2,0,4,4,4,3,3,0,0,3,1,3,83,0.83,""]
["Answers","24-h","Mortality defined as no response to gentle stimulation","LC50","","","5 reps","OECD protocol","Adult (body length = 2-3.5 cm, wet weight = 1.5-2.5 g) ","dechlorinated tap water","static renewal every 48 h","total Fe measured at beginning","","stated","Litchfield and Wilcoxon and probit paper","3790 - 79560 µg/L 95% CL",6.5,"20 mg/L as CaCO3","","","6.3 mg/L","28 - 30 oC","analytical grade reagents"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","210-2"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,0,3,3,0,0,3,1,3,81,0.81,""]
["Answers","48-h","Mortality defined as no response to gentle stimulation","LC50","","","5 reps","OECD protocol","Adult (body length = 2-3.5 cm, wet weight = 1.5-2.5 g) ","dechlorinated tap water","static renewal every 48 h","total Fe measured at beginning and end","","stated","Litchfield and Wilcoxon and probit paper"," 95% CL could not be determined",6.5,"20 mg/L as CaCO3","","","6.3 mg/L","28 - 30 oC","analytical grade reagents"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","210-3"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,0,3,3,0,0,3,1,3,81,0.81,""]
["Answers","72-h","Mortality defined as no response to gentle stimulation","LC50","","","5 reps","OECD protocol","Adult (body length = 2-3.5 cm, wet weight = 1.5-2.5 g) ","dechlorinated tap water","static renewal every 48 h","total Fe measured at beginning and 48 h","","stated","Litchfield and Wilcoxon and probit paper"," 95% CL could not be determined",6.5,"20 mg/L as CaCO3","","","6.3 mg/L","28 - 30 oC","analytical grade reagents"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","210-4"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,0,3,3,0,0,3,1,3,81,0.81,""]
["Answers","96-h","Mortality defined as no response to gentle stimulation","LC50","","","5 reps","OECD protocol","Adult (body length = 2-3.5 cm, wet weight = 1.5-2.5 g) ","dechlorinated tap water","static renewal every 48 h","total Fe measured at beginning and every 48 h with the renewal","","stated","Litchfield and Wilcoxon and probit paper","35 - 4460 µg/L 95% CL ",6.5,"20 mg/L as CaCO3","","","6.3 mg/L","28 - 30 oC","analytical grade reagents"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","210-5"]
["Scores",10,10,5,5,5,5,2,5,5,4,2,0,4,4,0,3,3,0,0,3,1,3,79,0.79,""]
["Answers","24-h","Mortality defined as no response to gentle stimulation","LC50","","","5 reps","OECD protocol","Adult (body length = 2-3.5 cm, wet weight = 1.5-2.5 g) ","dechlorinated tap water","static renewal every 48 h","total Fe measured at beginning and every 48 h with the renewal","","stated","Litchfield and Wilcoxon and probit paper","7580 - 35660 µg/L 95% CL ",6.5,"20 mg/L as CaCO3","","","6.3 mg/L","28 - 30 oC","analytical grade reagents"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","210-6"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,0,3,3,0,0,3,1,3,81,0.81,""]
["Answers","48-h","Mortality defined as no response to gentle stimulation","LC50","","","5 reps","OECD protocol","Adult (body length = 2-3.5 cm, wet weight = 1.5-2.5 g) ","dechlorinated tap water","static renewal every 48 h","total Fe measured at beginning and every 48 h with the renewal","","stated","Litchfield and Wilcoxon and probit paper","4020 - 20510 µg/L 95% CL ",6.5,"20 mg/L as CaCO3","","","6.3 mg/L","28 - 30 oC","analytical grade reagents"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","210-7"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,0,3,3,0,0,3,1,3,81,0.81,""]
["Answers","72-h","Mortality defined as no response to gentle stimulation","LC50","","","5 reps","OECD protocol","Adult (body length = 2-3.5 cm, wet weight = 1.5-2.5 g) ","dechlorinated tap water","static renewal every 48 h","total Fe measured at beginning and every 48 h with the renewal","","stated","Litchfield and Wilcoxon and probit paper","930 - 16590 µg/L 95% CL ",6.5,"20 mg/L as CaCO3","","","6.3 mg/L","28 - 30 oC","analytical grade reagents"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","210-8"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,0,3,3,0,0,3,1,3,81,0.81,""]
["Answers","96-h","Mortality defined as no response to gentle stimulation","LC50","","","5 reps","OECD protocol","Adult (body length = 2-3.5 cm, wet weight = 1.5-2.5 g) ","dechlorinated tap water","static renewal every 48 h","total Fe measured at beginning and every 48 h with the renewal","","stated","Litchfield and Wilcoxon and probit paper","470 - 2570 µg/L 95% CL ",6.5,"20 mg/L as CaCO3","","","6.3 mg/L","28 - 30 oC","analytical grade reagents"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","211-1"]
["Scores",10,10,5,5,5,5,2,5,5,4,2,0,0,4,4,3,3,0,0,3,1,3,79,0.79,""]
["Answers","24-h","Mortality as defined as no response with physical prodding or no movement when placed in a dish for a few minutes","LC50","","","2 reps","APHA protocol","Adult = 1.54-2 cm body length, 22.5 mg wet weight","dechlorinated tap water","static flow through","total Fe measured at beginning and every 48 h","","","Litchfield and Wilcoxon on probit paper","25740 - 133990 µg/L 95% CL",6.5,"18.7 mg/L as CaCO3","","","6.1 mg/L","28 - 30 oC","analytical grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","211-2"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,0,4,4,3,3,0,0,3,1,3,81,0.81,""]
["Answers","48-h","Mortality as defined as no response with physical prodding or no movement when placed in a dish for a few minutes","LC50","","","2 reps","APHA protocol","Adult = 1.54-2 cm body length, 22.5 mg wet weight","dechlorinated tap water","static flow through","total Fe measured at beginning and every 48 h","","","Litchfield and Wilcoxon on probit paper","10520 - 88850 µg/L 95 % CL",6.5,"18.7 mg/L as CaCO3","","","6.1 mg/L","28 - 30 oC","analytical grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","211-3"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,0,4,4,3,3,0,0,3,1,3,81,0.81,""]
["Answers","72-h","Mortality as defined as no response with physical prodding or no movement when placed in a dish for a few minutes","LC50","","","2 reps","APHA protocol","Adult = 1.54-2 cm body length, 22.5 mg wet weight","dechlorinated tap water","static flow through","total Fe measured at beginning and every 48 h","","","Litchfield and Wilcoxon on probit paper","4090 - 29470 µg/L 95 % CL",6.5,"18.7 mg/L as CaCO3","","","6.1 mg/L","28 - 30 oC","analytical grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","211-4"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,0,4,4,3,3,0,0,3,1,3,81,0.81,""]
["Answers","96-h","Mortality as defined as no response with physical prodding or no movement when placed in a dish for a few minutes","LC50","","","2 reps","APHA protocol","Adult = 1.54-2 cm body length, 22.5 mg wet weight","dechlorinated tap water","static flow through","total Fe measured at beginning and every 48 h","","","Litchfield and Wilcoxon on probit paper","1580 - 15250 µg/L 95 % CL",6.5,"18.7 mg/L as CaCO3","","","6.1 mg/L","28 - 30 oC","analytical grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-1"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,0,1,3,3,0,3,1,0,73,0.73,"The highest possible grade for this datum is acceptable."]
["Answers","24-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","Not applicable","measured pH in media but only stated that it was measured for test solutions","12 mg/L CaCO3 in the media","12 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-2"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,0,1,3,3,0,3,1,0,73,0.73,"The highest possible grade for this datum is acceptable."]
["Answers","48-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","Not applicable","measured pH in media but only stated that it was measured for test solutions","12 mg/L CaCO3 in the media","12 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-3"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,4,1,3,3,0,3,1,0,77,0.77,"The highest possible grade for this datum is acceptable."]
["Answers","72-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","32000 - 64000 µg/L 95% CL","measured pH in media but only stated that it was measured for test solutions","12 mg/L CaCO3 in the media","12 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-4"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,4,1,3,3,0,3,1,0,77,0.77,"The highest possible grade for this datum is acceptable."]
["Answers","96-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","12880 - 24820 µg/L 95% CL","measured pH in media but only stated that it was measured for test solutions","12 mg/L CaCO3 in the media","12 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-5"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,4,1,3,3,0,3,1,0,77,0.77,"The highest possible grade for this datum is acceptable."]
["Answers","24-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","29220 - 40690 µg/L 95% CL","measured pH in media but only stated that it was measured for test solutions","45 mg/L CaCO3 in the media","33 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-6"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,4,1,3,3,0,3,1,0,77,0.77,"The highest possible grade for this datum is acceptable."]
["Answers","48-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","23670 - 30490 µg/L 95% CL","measured pH in media but only stated that it was measured for test solutions","45 mg/L CaCO3 in the media","33 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-7"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,4,1,3,3,0,3,1,0,77,0.77,"The highest possible grade for this datum is acceptable."]
["Answers","72-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","22770 - 31610 µg/L 95% CL","measured pH in media but only stated that it was measured for test solutions","45 mg/L CaCO3 in the media","33 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-8"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,4,1,3,3,0,3,1,0,77,0.77,"The highest possible grade for this datum is acceptable."]
["Answers","72-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","21520 - 29810 µg/L 95% CL","measured pH in media but only stated that it was measured for test solutions","45 mg/L CaCO3 in the media","33 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-9"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,4,1,3,3,0,3,1,0,77,0.77,"The highest possible grade for this datum is acceptable."]
["Answers","24-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","41470 - 82590 µg/L 95% CL","measured pH in media but only stated that it was measured for test solutions","173 mg/L CaCO3 in the media","115 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-10"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,4,1,3,3,0,3,1,0,77,0.77,"The highest possible grade for this datum is acceptable."]
["Answers","48-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","36390 - 66520 µg/L 95% CL","measured pH in media but only stated that it was measured for test solutions","173 mg/L CaCO3 in the media","115 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-11"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,4,1,3,3,0,3,1,0,77,0.77,"The highest possible grade for this datum is acceptable."]
["Answers","72-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","18360 - 56090 µg/L 95% CL","measured pH in media but only stated that it was measured for test solutions","173 mg/L CaCO3 in the media","115 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-12"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,4,1,3,3,0,3,1,0,77,0.77,"The highest possible grade for this datum is acceptable."]
["Answers","96-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","18360 - 56090 µg/L 95% CL","measured pH in media but only stated that it was measured for test solutions","173 mg/L CaCO3 in the media","115 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-13"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,4,1,3,3,0,3,1,0,77,0.77,"The highest possible grade for this datum is acceptable."]
["Answers","24-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","117940 - 143040 µg/L 95% CL","measured pH in media but only stated that it was measured for test solutions","305 mg/L CaCO3 in the media","225 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-14"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,4,1,3,3,0,3,1,0,77,0.77,"The highest possible grade for this datum is acceptable."]
["Answers","48-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","117940 - 143040 µg/L 95% CL","measured pH in media but only stated that it was measured for test solutions","305 mg/L CaCO3 in the media","225 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-15"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,4,1,3,3,0,3,1,0,77,0.77,"The highest possible grade for this datum is acceptable."]
["Answers","72-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","97820 - 12190 µg/L 95% CL","measured pH in media but only stated that it was measured for test solutions","305 mg/L CaCO3 in the media","225 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","212-16"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,0,4,4,4,1,3,3,0,3,1,0,77,0.77,"The highest possible grade for this datum is acceptable."]
["Answers","96-h","Mortality - did not respond to gentle prodding","EC50 but really LC50","","","2 reps","No greater than 10% mortality in controls and referred to APHA","","Synthetic water at different hardness","Static non-renewal","","","","Moving average method - is o.k. but outdated","97820 - 12190 µg/L 95% CL","measured pH in media but only stated that it was measured for test solutions","305 mg/L CaCO3 in the media","225 mg/L in the media","",">5 mg/L","20 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","213-1"]
["Scores",10,0,5,5,5,5,2,0,5,0,0,4,0,4,4,0,0,0,0,0,1,3,53,0.53,"The highest possible grade for this datum is acceptable."]
["Answers","24-h","","EC50","","","3 reps","ISO 6341 and SFS 5062 protocols cited for methods","","ISO dilution water","","","","","Inferred by reference to ISO protocol","15000  - 16000 µg/L 95% CL","","","","","","25 oC","Analytical reagent grade of FeCl3.6H2O"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","214-1"]
["Scores",10,10,0,0,5,0,0,5,5,4,0,0,0,4,0,1,0,0,0,0,1,3,48,0.48,"The highest possible grade for this datum is acceptable."]
["Answers","48-h","mortality - lack of response when gently touched","Used hypothesis testing but did not define effect concentrations","","","1 replicate","","Freshly collected glochidia","River water from a reference site","static renewal","","","","hypothesis testing","","","","","","","6 oC","280 µg/L background Fe in river water used as dilution water"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","215-1"]
["Scores",10,5,5,0,5,5,5,5,5,0,2,0,4,4,0,1,1,0,0,0,1,0,58,0.57999999999999996,""]
["Answers","48-h","Immobilisation","NOEC","","","","control mortality <10%","neonate but no age","EPA media","","Measured at the beginning of the test","","graphed","Only stated the use of statistica software for NOEC","","","","","","","25 oC","Used Fe(NO3)2 salt"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","215-2"]
["Scores",10,5,5,0,5,5,5,5,5,0,2,0,4,4,0,1,1,0,0,0,1,0,58,0.57999999999999996,""]
["Answers","48-h","Immobilisation","LC50","","","","control mortality <10%","neonate but no age","EPA media","","Measured at the beginning of the test","","graphed","Only stated the use of statistica software to do probit anlaysis for LC50","","","","","","","25 oC","Used Fe(NO3)2 salt"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","217-1"]
["Scores",10,5,5,5,5,0,2,0,5,4,4,0,0,4,0,3,3,3,0,3,3,0,64,0.64,""]
["Answers","96-h","","TL50 which is equivalent to LC50","","","Not stated but seems likely","APHA","Larvae collected from stream","Tap water from Lake Superior","static","Measured using polargraph and found to be within 5% of nominal","","","Followed APHA method but statistics were not mentioned in paper","","7.25 - 8.2","44 - 48 mg/L","40 - 54 mg/L","","9.2 - 8.0 mg/L","18 - 18.5 oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","218-1"]
["Scores",10,5,0,0,5,5,0,5,5,4,4,0,4,0,0,3,3,3,0,3,3,0,62,0.62,""]
["Answers","5 months","","No EC/LC/NOECs calculated in the paper so I used the raw data in the appendix and calculated in Toxcalc","","","2 reps","","3 months old, average weight of 0.4 g","Well water","flow-through","Measured Fe(II) and Fe(III), total and dissolved","","Used data in appendix in Toxcalc to calculate concentration response curve","Statistics not used in the publication, but using the raw data in the appendix I performed the statistical analysis in Toxcalc using probit","Was able to calculate this in Toxcalc from raw data (2954 - 15470 µg/L 95% CL)","6.86 - 7.16","139  - 238 mg/L as CaCO3","79 - 101 mg/L as CaCO3","","90.5 - 96.1 % sat.","24o C","Not stated"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","218-2"]
["Scores",10,5,0,0,5,5,0,5,5,4,4,0,4,0,0,3,3,3,0,3,3,0,62,0.62,""]
["Answers","5 months","","No EC/LC/NOECs calculated in the paper so I used the raw data in the appendix and calculated in Toxcalc","","","2 reps","","3 months old, average weight of 0.4 g","Well water","flow-through","Measured Fe(II) and Fe(III), total and dissolved","","Used data in appendix in Toxcalc to calculate concentration response curve","Statistics not used in the publication, but using the raw data in the appendix I performed the statistical analysis in Toxcalc using probit","Was able to calculate this in Toxcalc from raw data (25862 - 51697 µg/L 95% CL)","6.86 - 7.16","139  - 238 mg/L as CaCO3","79 - 101 mg/L as CaCO3","","90.5 - 96.1 % sat.","24o C","Not stated"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","217-2"]
["Scores",10,5,5,5,5,0,2,0,5,4,4,0,0,4,0,3,3,3,0,3,3,0,64,0.64,""]
["Answers","9 d","mortality","LC50","","","Not stated","APHA stated for test method and statistical methods","","Lake Superior water carbon filtered","Static","Measured with polarograph at beginning and 2 weeks later","","","Used APHA method for statistics but not stated","","7.25 - 7.7 measured at beginning and end","44 - 48 mg/L","40 - 72 mg/L","","9.2 - 8 mg/L","18 - 18.5 oC","not stated"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","217-3"]
["Scores",10,5,5,5,5,0,2,0,5,4,4,0,0,4,0,3,3,3,0,3,3,0,64,0.64,""]
["Answers","7 d","mortality","LC50","","","Not stated","APHA stated for test method and statistical methods","Must be larvae","Lake Superior water carbon filtered","Static","Measured with polarograph at beginning and 2 weeks later","","","Used APHA method for statistics but not stated","","7.25 - 8.1 measured at beginning and end","44 - 50 mg/L","40 - 46 mg/L","","9.2 - 8 mg/L","18 - 18.5 oC","not stated"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","218-3"]
["Scores",10,0,0,0,5,5,0,5,5,4,4,0,4,0,0,3,3,3,0,3,3,0,57,0.56999999999999995,""]
["Answers","1 year","","","","","2 reps","","3 months (34 mm in length)","Well water","Flow-through and mixed to keep precipitate in suspension","Iron speciation (total, dissolved, Fe2+) measured at regular intervals","","In tables","No statistical tests of significance used","","7.0 - 7.3","128 - 214 mg/L hardness","74 - 113 mg/L","","85 - 91 % saturation","15 oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","218-4"]
["Scores",10,0,0,0,5,5,0,5,5,4,4,0,4,0,0,3,3,3,0,3,3,0,57,0.56999999999999995,""]
["Answers","1 year","","","","","2 reps","","3 months (34 mm in length)","Well water","Flow-through and mixed to keep precipitate in suspension","Iron speciation (total, dissolved, Fe2+) measured at regular intervals","","In tables","No statistical tests of significance used","","7.0 - 7.3","128 - 214 mg/L hardness","74 - 113 mg/L","","85 - 91 % saturation","15 oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","218-5"]
["Scores",10,0,0,0,5,5,0,5,5,4,4,0,4,0,0,3,3,3,0,3,3,0,57,0.56999999999999995,""]
["Answers","1 year","","","","","2 reps","","3 months (34 mm in length)","Well water","Flow-through and mixed to keep precipitate in suspension","Iron speciation (total, dissolved, Fe2+) measured at regular intervals","","In tables","No statistical tests of significance used","","7.0 - 7.3","128 - 214 mg/L hardness","74 - 113 mg/L","","85 - 91 % saturation","15 oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","218-6"]
["Scores",10,0,0,0,5,5,0,5,5,4,4,0,4,0,0,3,3,3,0,3,3,0,57,0.56999999999999995,""]
["Answers","14 weeks","","","","","2 reps","","Adults (1:1 male:female)","Well water","Flow-through and mixed to keep precipitate in suspension","Iron speciation (total, dissolved, Fe2+) measured at regular intervals","","In tables","No statistical tests of significance used","","7.1 - 7.6","112 - 256 mg/L hardness","70 - 108 mg/L","",">75 % saturation","18oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","218-7"]
["Scores",0,0,0,0,5,5,0,5,5,4,4,0,4,0,0,3,3,3,0,3,3,0,47,0.47,""]
["Answers","","","","","","2 reps","","Adults (1:1 male:female)","Well water","Flow-through and mixed to keep precipitate in suspension","Iron speciation (total, dissolved, Fe2+) measured at regular intervals","","In tables","No statistical tests of significance used","","7.1 - 7.6","112 - 256 mg/L hardness","70 - 108 mg/L","",">75 % saturation","18oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","219-1"]
["Scores",10,0,0,0,5,5,0,5,5,4,4,0,4,4,0,3,3,3,0,3,3,0,61,0.61,""]
["Answers","35 weeks as shown in results table 5","","Inferred from statistical test conducted that it was a NOEC","","","2 reps","","3 months (average length 34 mm)","Well water","Flow-through","Iron speciation (dissolved, total, Fe2+) measured at regular intervals","","","Test for significant differences in slope","","6.5 - 7.9","102 - 236 mg/L","55 - 138 mg/L","","79.6 - 97.8 % saturation","8.5 - 21.6 oC",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","220-1"]
["Scores",10,0,0,0,5,5,0,5,5,4,4,0,4,0,0,3,3,3,0,3,3,0,57,0.56999999999999995,""]
["Answers","12 months given in abstract and table titles","","","","","2 reps","","3 months (average weight 0.4g)","well water","flow-through","Iron speciation (total, filtered, Fe2+) done throughout the test","","","Test for differences in slope","","6.9 - 7.2","139 - 238 mg/L hardness","79 - 101 mg/L CaCO3","","90.5 - 96.1 % saturation","22.6 - 24.8 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","220-2"]
["Scores",10,0,0,0,5,5,0,5,5,4,4,0,4,0,0,3,3,3,0,3,3,0,57,0.56999999999999995,""]
["Answers","1-5 months given in table 5 of results","","","","","2 reps","","3 months (average weight 0.4g)","well water","flow-through","Iron speciation (total, filtered, Fe2+) done throughout the test","","","","","6.9 - 7.2","139 - 238 mg/L hardness","79 - 101 mg/L CaCO3","","90.5 - 96.1 % saturation","22.6 - 24.8 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","220-3"]
["Scores",10,0,0,0,5,5,0,5,5,4,4,0,4,0,0,3,3,3,0,3,3,0,57,0.56999999999999995,""]
["Answers","12 months given  on Table 3","","","","","2 reps","","3 months (average weight 0.4g)","well water","flow-through","Iron speciation (total, filtered, Fe2+) done throughout the test","","","","","6.9 - 7.2","139 - 238 mg/L hardness","79 - 101 mg/L CaCO3","","90.5 - 96.1 % saturation","22.6 - 24.8 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","220-4"]
["Scores",10,0,0,0,5,5,0,5,5,4,4,0,4,0,0,3,3,3,0,3,3,0,57,0.56999999999999995,""]
["Answers","30 d given at Table 4","","","","","2 reps","","30 d post hatch","well water","flow-through","Iron speciation (total, filtered, Fe2+) done throughout the test","","","","","6.9 - 7.2","139 - 238 mg/L hardness","79 - 101 mg/L CaCO3","","90.5 - 96.1 % saturation","22.6 - 24.8 oC","Reagent grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","221-1"]
["Scores",10,0,0,0,5,5,0,5,5,4,4,0,4,0,0,3,3,3,0,3,0,0,54,0.54,""]
["Answers","19 months in table 4","","","","","2 reps","","3 month old (average length = 34 mm)","Well water","flow-through","iron speciation (filtered, total, Fe2+) measured throughout the experiment","","","No statistics were used","","7 - 7.3","128 - 228 mg/L","74 - 113 mg/L","","77.5 - 91.3 % sat","",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","221-2"]
["Scores",10,0,0,0,5,5,0,5,5,4,4,0,4,0,0,3,3,3,0,3,0,0,54,0.54,""]
["Answers","5 months in table 4","","","","","2 reps","","1 year","Well water","flow-through","iron speciation (filtered, total, Fe2+) measured throughout the experiment","","","No statistics were used","","7 - 7.3","128 - 228 mg/L","74 - 113 mg/L","","77.5 - 91.3 % sat","",""]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","222-1"]
["Scores",10,0,0,0,5,5,0,5,5,4,4,0,4,0,0,3,3,0,0,3,3,0,54,0.54,""]
["Answers","30 d post-hatch","","","","","2 replicates","","30 d post hatch","well water","flow-through","iron speciation (dissolved, total, Fe2+)","","","no statistics used","","7.76 - 8.12","159 - 180 mg/L gradient as a result of neutralizing Fe with Ca(OH)2","","","93.6 - 100 % saturation","10.9 - 11.6 oC",""]

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[null,1,"Fill in the blank cells below, based on information provided in the paper being assessed. You can either pick an option from the dropdown list or add the appropriate detail using the 'Add to list' button. ",null,null,null,null,null,null,null,null,null,null,null,"* All the text in RED are used for drop down list ,make changes with caution."]
[null,null,"Category",{"$f":"IF(AF3=\"\",0,1)","v":1},"Freshwater/metal/plant",{"$f":"IF(E3=\"\",0,1)","v":1},"Citation No.",{"$f":"IF(ISERROR(FIND(\"-\",E3)),E3,LEFT(E3,FIND(\"-\",E3)-1))","v":"216"},null,"Taxonomic group",null,null,null,null,"Species",null,null,"Lifestage",null,null,"Exposure duration",null,null,"Measure of toxicity",null,null,"Toxicity endpoint",null,null,"Taxonomic group",null,null,"Phylum",null,null,"Class",null,null,"Media",null,null,"Temperature ",null,null,"Acute Or Chronic",null,null,"PH",null,null,"Salinity/Comdictivity"]
[null,null,"Paper and data unique identifier(eg. 1-1,10-150)",{"$f":"IF(AI3=\"\",0,1)","v":1},"216-1",{"$f":"IF(E4=\"\",0,1)","v":1},"Species (Scientific name)",null,null,"Phylum",null,null,null,null,"Aedes albopictus",13,{"$f":"IF(P3=0,\"\",INDEX(O3:O108,P3))","v":"Lemna minor"},"1 month",3,{"$f":"IF(S3=0,\"\",INDEX(R3:R108,S3))","v":"frond"},"11 hour",8,{"$f":"IF(V3=0,\"\",INDEX(U3:U108,V3))","v":"96 h"},"96 h",2,{"$f":"IF(Y3=0,\"\",INDEX(X3:X108,Y3))","v":"EC50"},"growth",13,{"$f":"IF(AB3=0,\"\",INDEX(AA3:AA108,AB3))","v":"Growth (frond number)"},"Ascomycota",4,{"$f":"IF(AE3=0,\"\",INDEX(AD3:AD108,AE3))","v":"Macrophyte"},"Ascomycota",5,{"$f":"IF(AH3=0,\"\",INDEX(AG3:AG108,AH3))","v":"Tracheophyta"},"Ascomycetes",6,{"$f":"IF(AK3=0,\"\",INDEX(AJ3:AJ108,AK3))","v":"Liliopsida"},"Freshwater",1,{"$f":"IF(AN3=0,\"\",INDEX(AM3:AM108,AN3))","v":"Freshwater"},"14 (night) 20 (day)°C",9,{"$f":"IF(AQ3=0,\"\",INDEX(AP3:AP108,AQ3))","v":"27 oC"},"Acute ",1,{"$f":"IF(AT3=0,\"\",INDEX(AS3:AS108,AT3))","v":"Acute "},6,10,{"$f":"IF(AW3=0,\"\",INDEX(AV3:AV108,AW3))","v":"not recorded"},"Salinity1",null,"N.A."]
[null,null,"Citation",{"$f":"IF(AL3=\"\",0,1)","v":1},"Wang W (1986). Toxicity tests of aquatic pollutants by using common duckweed. Environmental Pollution Series B, Chemical and Physical, 11(1), 1-14. ",{"$f":"IF(H8=\"\",0,1)","v":1},"Lifestage",null,null,"Class",null,null,null,null,"Alatospora acuminata",null,null,"1 year old",null,null,"13 days",null,null,"EC50",null,null,"growth (biomass)",null,null,"Fish ",null,null,"Charophyta",null,null,"Branchiopoda",null,null,"Marinewater",null,null,"15°C",null,null,"Chronic",null,null,7,null,null,"Salinity2"]
[null,null,null,{"$f":"IF(AO3=\"\",0,1)","v":1},null,{"$f":"IF(AX3=\"\",0,1)","v":1},"Exposure duration",null,null,"Media",null,null,null,null,"Articulospora tetracladia",null,null,"frond",null,null,"21 d",null,null,"LC50",null,null,"Growth (dry weight)",null,null,"Fungi",null,null,"Chlorophyta",null,null,"Chlorophyceae",null,null,"N.A.",null,null,"20°C",null,null,"N.A.",null,null,">5"]
[null,null,null,{"$f":"IF(AR3=\"\",0,1)","v":1},null,{"$f":"IF(Q3=\"\",0,1)","v":1},"Measure of toxicity",null,null,"Temperature ",null,null,null,null,"Australian Bass",null,null,"N.A.",null,null,"3-4 months",null,null,"LOEC",null,null,"Growth (dry weight, rhizomes)",null,null,"Macrophyte",null,null,"N.A.",null,null,"Equisetopsida",null,null,"SIS media",null,null,"22-24",null,null,null,null,null,"7.5 at EC50"]
[null,null,null,{"$f":"IF(AU3=\"\",0,1)","v":1},null,{"$f":"IF(T3=\"\",0,1)","v":1},"Toxicity endpoint",null,null,"Acute Or Chronic ",null,null,null,null,"Chlorella vulgaris",null,null,"Not reported",null,null,"64 d",null,null,"N.A.",null,null,"Growth (dry weight, roots)",null,null,"Microalga",null,null,"Tracheophyta",null,null,"Leotiomycetes",null,null,null,null,null,"24°C",null,null,null,null,null,"7.5-8.1"]
[null,null,null,null,null,{"$f":"IF(W3=\"\",0,1)","v":1},"Toxicity value","3700 µg/L (nominal)",null,"PH",null,null,null,null,"Lactuca sativa",null,null,"seed",null,null,"7 days",null,null,"NOEC",null,null,"Growth (dry weight, shoots)",null,null,"N.A.",null,null,null,null,null,"Liliopsida",null,null,null,null,null,"25°C",null,null,null,null,null,"N.A."]
[null,2,"Select the appropriate answer option to each question. Comments can be added to the cells in column H.",null,null,{"$f":"IF(Z3=\"\",0,1)","v":1},null,null,null,"Salinity/Conductivity","N.A.",null,null,null,"Lemna disperma",null,null,"seedling",null,null,"72 h",null,null,null,null,null,"Growth (root length)",null,null,null,null,null,null,null,null,"N.A.",null,null,null,null,null,"N.A.",null,null,null,null,null,"N.R."]
[null,"*","If the selected answer option does not work try clicking on any other cell first and then click back on the answer option again.",null,null,{"$f":"IF(AC3=\"\",0,1)","v":1},null,null,null,null,null,null,null,null,"N.A.",null,null,null,null,null,"96 h",null,null,null,null,null,"Growth (root:shoot ratio)",null,null,null,null,null,null,null,null,"Trebouxiophyceae",null,null,null,null,null,"not reported",null,null,null,null,null,"PH11"]
[null,"No.","Question","Marks Awarded","Answer Options","Option References","Scores","Answers",null,null,null,null,null,null,"Phragmites australis",null,null,null,null,null,"N.A.",null,null,null,null,null,"Mortality",null,null,null,null,null,null,null,null,null,null,null,null,null,null,"27 oC",null,null,null,null,null,"PH7.0 "]
[null,null,"Was the duration of the exposure stated (e.g. 48 or 96 h)?",10,null,1,{"$f":"IF(F15=0,0,INDEX($D12:$D13,F12,1))","v":10},"96-h",null,null,null,null,null,null,"Pseudokirchneriella subcapitata",null,null,null,null,null,null,null,null,null,null,null,"N.A.",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"not recorded"]
[null,1,null,0,null,null,null,null,null,null,null,null,null,null,"Selenastrum capricornutum",null,null,null,null,null,null,null,null,null,null,null,"Not reported"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Tetrachaetum elegans",null,null,null,null,null,null,null,null,null,null,null,"Reproduction"]
[null,null,"Was the biological endpoint (e.g., immobilization or population growth) stated and defined (10 marks)? Award 5 marks if the biological endpoint is only stated.",10,null,1,{"$f":"IF(F15=0,0,INDEX($D15:$D17,F15,1))","v":10},"Growth rate defined by number of fronds",null,null,null,null,null,null,"Lemna minor",null,null,null,null,null,null,null,null,null,null,null,"Growth (frond number)"]
[null,2,null,5]
[null,null,null,0]
[null,null,"Was the biological effect stated (e.g. LC or NOEC)?",5,null,1,{"$f":"IF(F18=0,0,INDEX($D18:$D19,F18,1))","v":5},"EC50"]
[null,3,null,0]
[]
[null,4,"Was the biological effect quantified (e.g. 50% effect, 25% effect)? \nNote: The effect for NOEC and LOEC data must be quantified.",5,null,1,{"$f":"IF(F21=0,0,INDEX($D21:$D22,F21,1))","v":5}]
[null,null,null,0]
[null,5,"Were appropriate controls (e.g. a no-toxicant control and/or solvent control) used?",5,null,1,{"$f":"IF(F23=0,0,INDEX($D23:$D24,F23,1))","v":5}]
[null,null,null,0]
[]
[null,6,"Was each control and contaminant concentration at least duplicated?",5,null,1,{"$f":"IF(F26=0,0,INDEX($D26:$D27,F26,1))","v":5},"3-6 reps"]
[null,null,null,0]
[]
[null,7,"Were test acceptability criteria stated (e.g. mortality in controls must not exceed a certain percentage) (5 marks)?\nOR Were test acceptability criteria inferred (e.g. test method used [USEPA, OECD, ASTM etc.]) (award 2 marks). \nNote: Data that fail the acceptability criteria are automatically deemed to be of unacceptable quality and must not be used. ",5,null,3,{"$f":"IF(F29=0,0,INDEX($D29:$D31,F29,1))","v":0}]
[null,null,null,2]
[null,null,null,0]
[null,8,"Were the characteristics of the test organism (e.g. length, mass, age) stated?",5,null,2,{"$f":"IF(F32=0,0,INDEX($D32:$D34,F32,1))","v":0}]
[null,null,null,0]
[]
[null,9,"Was the type of test media used stated?",5,null,1,{"$f":"IF(F35=0,0,INDEX($D35:$D37,F35,1))","v":5},"Standard Methods (1980)"]
[null,null,null,0]
[]
[null,null,"Was the type of exposure (e.g. static, flow through) stated? ",4,null,2,{"$f":"IF(F38=0,0,INDEX($D38:$D40,F38,1))","v":0}]
[null,10,null,0]
[]
[null,null,"Were the contaminant concentrations measured at the beginning and end of the exposure (4 marks)? Award 2 marks if they were measured only once during the test.\nNote: If the concentrations were not measured then automatically the data cannot be high quality. ",4,null,3,{"$f":"IF(F41=0,0,INDEX($D41:$D43,F41,1))","v":0}]
[null,11,null,2]
[null,null,null,0]
[null,null,"Were parallel reference toxicant toxicity tests conducted?",4,null,2,{"$f":"IF(F44=0,0,INDEX($D44:$D46,F44,1))","v":0}]
[null,12,null,0]
[]
[null,null,"Was there a concentration–response relationship either observable or stated?",4,null,1,{"$f":"IF(F47=0,0,INDEX($D47:$D49,F47,1))","v":4},"Stated"]
[null,13,null,0]
[]
[null,null,"Was an appropriate statistical method or model used to determine the toxicity?\nNote: They should be accepted by a recognised national or international regulatory body (e.g. USEPA, OECD, ASTM). ",4,null,2,{"$f":"IF(F50=0,0,INDEX($D50:$D52,F50,1))","v":0},"not stated"]
[null,14,null,0]
[]
[null,15,"For LC/EC/BEC data was an estimate of variability provided? \nOR \nFor NO EC/LOEC data was the significance level 0.05 or less?  \n\n",4,null,2,{"$f":"IF(F53=0,0,INDEX($D53:$D55,F53,1))","v":0}]
[null,null,null,0]
[]
[null,16,"Were the following parameters measured and stated? (3 marks if measured and stated, 1 mark if just measured)."]
[null,null,null,3,null,3,{"$f":"IF(F57=0,0,INDEX($D57:$D59,F57,1))","v":0}]
[null,16.100000000000001,"pH ",1]
[null,null,null,0]
[null,null,null,3,null,3,{"$f":"IF(F60=0,0,INDEX($D60:$D62,F60,1))","v":0}]
[null,16.2,"Hardness",1]
[null,null,null,0]
[null,null,null,3,null,3,{"$f":"IF(F63=0,0,INDEX($D63:$D65,F63,1))","v":0}]
[null,16.3,"Alkalinity ",1]
[null,null,null,0]
[null,null,null,3,null,3,{"$f":"IF(F66=0,0,INDEX($D66:$D68,F66,1))","v":0}]
[null,16.399999999999999,"Organic carbon concentration",1]
[null,null,null,0]
[null,null,"Was the temperature measured and stated (3 marks)? Award 1 mark if the temperature was measured but not stated or if only the temperature settings of the room or chamber are stated.",3,null,2,{"$f":"IF(F69=0,0,INDEX($D69:$D71,F69,1))","v":1},"27 oC"]
[null,17,null,1]
[null,null,null,0]
[null,null,"Were test solutions, blanks and/or controls tested for contamination OR were analytical reagent grade chemicals used or the highest possible purity chemicals used for the experiment? (3 marks).",3,null,2,{"$f":"IF(F72=0,0,INDEX($D72:$D73,F72,1))","v":0}]
[null,18,null,0]
[]
[null,null,null,null,null,null,null,null,{"$f":"(COUNTIF(F12:F73,\"=0\"))+COUNTIF(F2:F10,\"=0\")+COUNTIF(D2:D7,\"=0\")","v":0}]
[]
[]
[null,null,"Total score",null,null,null,{"$f":"IF(SUM(G12:G72)=0,\"\",SUM(G12:G72))","v":50}]
[null,null,"Total Max. possible score for the various types of data and contaminants ",null,{"$f":"D12+D15+D18+D21+D23+D26+D29+D32+D35+D38+D41+D44+D47+D50+D53+D57+D66+D69+D72+D60+D63","v":97}]
[null,null,"Quality Score",null,{"$f":"\"= [Total score / Total possible score] * 100 = \"","v":"= [Total score / Total possible score] * 100 = "},null,{"$f":"IF(ISERROR(G78/E79),#N/A,G78/E79)","v":0.51546391752577314},{"$f":"IF(F41=3,\"The highest possible grade for this datum is acceptable.\",\"\")","v":"The highest possible grade for this datum is acceptable."}]
[]
[null,null,"H = high quality                           Quality score ≥ 80%      "]
[null,null,"A = acceptable quality         > 50% Quality score < 80%      "]
[null,null,"U = unacceptable quality              Quality score ≤ 50%  "]
[]
[3,null,"Click the 'Save & Update' button to save and export the answers to the 'All results' worksheet. "]
[null,null,"Select the 'Reset Answers' button if you wish to clear all answers."]

{"sheet":"FW metal plant_WS","index":8,"n_rows":59,"n_cols":25,"rows_kept":59,"cut":false,"hidden":false,"merged":[]}
[]
["Question No.",1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16.100000000000001,16.2,16.3,16.399999999999999,17,18,"Total Score","Quality Score"]
["Short Questions","Duration of the exposure ","Biological endpoint","Biological effect stated or not","Biological effect quantified or not","Appropriate controls","Control & contaminant concentration","Test acceptability criteria","Characteristics of the test organism"," Type of test media","Type of exposure","Contaminant concentrations","Parallel reference toxicant toxicity tests","Concentration–response relationship","Determine the toxicity","NOEC/LOEC or LC/EC/BEC","pH","Hardness","Alkalinity ","Organic carbon concentration","Temperature","Grade or purity"]
["Marks Awarded ","10 or 0","10,5 or 0","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","4 or 0","4 or 2 or 0","4 or 2","4 or 2","4 or 2","4 or 2","3 or 1 or 0","3 or 1 or 0","3 or 1 or 0","3 or 1 or 0","3 or 0","3 or 0 "]
["Max. Possible Score",10,10,5,5,5,5,5,5,5,4,4,4,4,4,4,3,3,3,3,3,3,{"$f":"SUM(B5:V5)","v":97}]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","150-1"]
["Scores",10,10,5,5,5,5,5,0,5,4,0,4,0,4,0,1,0,0,0,1,3,67,0.69072164948453607,"The highest possible grade for this datum is acceptable."]
["Answers","7 days","Growth was determined as the increase in fronds over seven days.","EC","50, note: reported value was 1.8 mM, this was converted to µg/L","non-toxicant control","4 replicates","Seven divisions per initial frond in the\ncontrols (21 fronds per vial) over the week’s exposure, and temperature maintained\nbetween 24 ± 2°C. Also physico-chemical conditions had meet requirements.","","SIS medium, OECD 2006","Static","","Standard MeS tests done in parraelel with each sample test (assessing effectiveness of MeS degradation).","","Toxic equivalents were calculated  using an adaptation of the method described in Leusch et al.(2006).","","7.0 ± 0.1. Stated as being measured at beginning and end of test, but not reported.","","","","24 ± 2 °C","AR Grade"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","153-1"]
["Scores",10,10,5,5,5,5,0,5,0,4,0,0,4,4,4,1,0,0,0,1,0,63,0.64948453608247425,"The highest possible grade for this datum is acceptable."]
["Answers","64 d","Measurements of the longest root","NOEC","root length, tested at p = 0.05","non-toxicant controls","5 replicates","","44-d old Seedlings","nutrient solution","Renewal every 3 d","","","Graphically","one-way ANOVA followed by Tukeys test","p = 0.05",6,"","","","14 (night) 20 (day) °C",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","153-2"]
["Scores",10,10,5,5,5,5,0,5,0,4,0,0,4,4,4,1,0,0,0,1,0,63,0.64948453608247425,"The highest possible grade for this datum is acceptable."]
["Answers","64 d","Measurements of the longest root and longest shoot every 3d","NOEC","root length, tested at p = 0.05","non-toxicant controls","5 replicates","","44-d old Seedlings","nutrient solution","Renewal every 3 d","","","Graphically","one-way ANOVA followed by Tukeys test","p = 0.05",6,"","","","14 (night) 20 (day) °C",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","153-3"]
["Scores",10,10,5,5,5,5,0,5,0,4,0,0,4,4,4,1,0,0,0,1,0,63,0.64948453608247425,"The highest possible grade for this datum is acceptable."]
["Answers","64 d","Each plant was divided into roots, rhizomes, and shoots, each section dried at 40 °C for 3 days. The resulting material was placed in a desiccator and cooled to room temperature before weighing.","NOEC","shoot dry weight, tested at p = 0.05","non-toxicant controls","5 replicates","","44-d old Seedlings","nutrient solution","Renewal every 3 d","","","Graphically","one-way ANOVA followed by Tukeys test","p = 0.05",6,"","","","14 (night) 20 (day) °C",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","153-4"]
["Scores",10,10,5,5,5,5,0,5,0,4,0,0,4,4,4,1,0,0,0,1,0,63,0.64948453608247425,"The highest possible grade for this datum is acceptable."]
["Answers","64 d","Each plant was divided into roots, rhizomes, and shoots, each section dried at 40 °C for 3 days. The resulting material was placed in a desiccator and cooled to room temperature before weighing.","NOEC","root dry weight, tested at p = 0.05","non-toxicant controls","5 replicates","","44-d old Seedlings","nutrient solution","Renewal every 3 d","","","Graphically","one-way ANOVA followed by Tukeys test","p = 0.05",6,"","","","14 (night) 20 (day) °C",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","153-5"]
["Scores",10,10,5,5,5,5,0,5,0,4,0,0,4,4,4,1,0,0,0,1,0,63,0.64948453608247425,"The highest possible grade for this datum is acceptable."]
["Answers","64 d","Each plant was divided into roots, rhizomes, and shoots, each section dried at 40 °C for 3 days. The resulting material was placed in a desiccator and cooled to room temperature before weighing.","NOEC","rhizome dry weight, tested at p = 0.05","non-toxicant controls","5 replicates","","44-d old Seedlings","nutrient solution","Renewal every 3 d","","","Graphically","one-way ANOVA followed by Tukeys test","p = 0.05",6,"","","","14 (night) 20 (day) °C",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","154-1"]
["Scores",10,10,0,5,5,5,0,0,5,0,0,0,4,4,4,3,0,0,0,1,0,56,0.57731958762886593,"The highest possible grade for this datum is acceptable."]
["Answers","21 d","dry weight, samples washed three times with distilled water and then dried to constant weight at 60 °C.","But inferred from ANOVA results","dry weight, ","media controls, no toxicant","15 flasks per treatment","","","Basal medium, recipe supplied in a Table.","Assume static","Metals in media (no added Fe) were measured using AAS.","","Graphical","ANOVA and Tukeys.","p = 0.05","6.98-7.03","","","","15°C",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","154-2"]
["Scores",10,10,0,5,5,5,0,0,5,0,0,0,4,4,4,3,0,0,0,1,0,56,0.57731958762886593,"The highest possible grade for this datum is acceptable."]
["Answers","21 d","dry weight, samples washed three times with distilled water and then dried to constant weight at 60 °C.","But inferred from ANOVA results","dry weight, ","media controls, no toxicant","15 flasks per treatment","","","Basal medium, recipe supplied in a Table.","Assume static","Metals in media (no added Fe) were measured using AAS.","","Graphical","ANOVA and Tukeys.","p = 0.05","6.98-7.03","","","","15°C",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","154-3"]
["Scores",10,10,0,5,5,5,0,0,5,0,0,0,4,4,4,3,0,0,0,1,0,56,0.57731958762886593,"The highest possible grade for this datum is acceptable."]
["Answers","21 d","dry weight, samples washed three times with distilled water and then dried to constant weight at 60 °C.","But inferred from ANOVA results","dry weight, ","media controls, no toxicant","15 flasks per treatment","","","Basal medium, recipe supplied in a Table.","Assume static","Metals in media (no added Fe) were measured using AAS.","","Graphical","ANOVA and Tukeys.","p = 0.05","6.98-7.03","","","","15°C",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","168-1"]
["Scores",0,5,5,5,5,0,0,0,5,0,0,0,0,0,0,1,0,0,0,0,0,26,0.26804123711340205,"The highest possible grade for this datum is acceptable."]
["Answers","ambiguous - 3-4 months","growth rate - not defined","maximum concentration tolerated' considered equivalent to a NOEC","as above. Data in Tables.","non-toxicant controls","not stated","","states algae added at a density that was  'light green'","mineral media","","","","","","","media pH 'just below 7', but test solutons for Fe down to 1.5","","","","room temperature","Saltused not described."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","180-1"]
["Scores",10,5,0,5,5,0,0,0,5,0,0,0,4,4,0,1,0,0,0,1,0,40,0.41237113402061853,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","Growth rate","","First significant effect seen from data in Tables","Diluent Control","Unclear","","","Standard freshwater with reference to Gavaskar et al, 2005","","","","Observed","","not stated","7.5-8.1, however, unclear if this is in Fe tests or nanoFe tests only. Table of data for Nano-Fe only. No pH data for Fe exposure","","","","20°C",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","180-2"]
["Scores",10,5,0,5,5,0,0,0,5,0,0,0,4,4,0,1,0,0,0,1,0,40,0.41237113402061853,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","Growth rate","","First significant effect seen from data in Tables","Diluent Control","Unclear","","","Standard freshwater with reference to Gavaskar et al, 2005","","","","Observed","","not stated","7.5-8.1, however, unclear if this is in Fe tests or nanoFe tests only. Table of data for Nano-Fe only. No pH data for Fe exposure","","","","20°C",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","194-2"]
["Scores",10,5,5,5,5,5,2,0,5,4,2,0,4,4,0,3,0,0,0,1,3,63,0.64948453608247425,""]
["Answers","72 h","Growth rate","EC",50,"Diluent controls","3 replicates","OECD 201","1 x 10^4 cells/mL, but not known if exponentially growing.","OECD medium","Static","Unclear but measured at least once.","not stated","In tables","OECD guidelines, comprehensive toxicity data analysis version 5.0, Dunnett's test","","7.04-8.0 on Day 0, 4.74-7.64 on Day 2. 22 mg/L = pH 5.48 and 44 mg/L = pH 4.74 on Day 2. But at EC50, pH around 7.5.","","","","22-24°C","99% purity FeCl2"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","194-3"]
["Scores",10,5,5,5,5,5,2,0,5,4,2,0,4,4,0,3,0,0,0,1,3,63,0.64948453608247425,""]
["Answers","72 h","biomass","EC",50,"Diluent controls","3 replicates","OECD 201","1 x 10^4 cells/mL, but not known if exponentially growing.","OECD medium","Static","Unclear but measured at least once.","not stated","In tables","OECD guidelines, comprehensive toxicity data analysis version 5.0, Dunnett's test","","7.04-8.0 on Day 0, 4.74-7.64 on Day 2. 22 mg/L = pH 5.48 and 44 mg/L = pH 4.74 on Day 2. But at EC50, pH around 7.5.","","","","22-24°C","99% purity FeCl2"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","194-3"]
["Scores",10,5,5,5,5,5,2,0,5,4,2,0,4,4,0,3,0,0,0,1,3,63,0.64948453608247425,""]
["Answers","72 h","growth rate","NOEC","","Diluent controls","3 replicates","OECD 201","1 x 10^4 cells/mL, but not known if exponentially growing.","OECD medium","Static","Unclear but measured at least once.","not stated","In tables","OECD guidelines, comprehensive toxicity data analysis version 5.0, Dunnett's test","","7.04-8.0 on Day 0, 4.74-7.64 on Day 2. 22 mg/L = pH 5.48 and 44 mg/L = pH 4.74 on Day 2. But at EC50, pH around 7.5.","","","","22-24°C","99% purity FeCl2"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","194-4"]
["Scores",10,5,5,5,5,5,2,0,5,4,2,0,4,4,0,3,0,0,0,1,3,63,0.64948453608247425,""]
["Answers","72 h","growth rate","NOEC","","Diluent controls","3 replicates","OECD 201","1 x 10^4 cells/mL, but not known if exponentially growing.","OECD medium","Static","Unclear but measured at least once.","not stated","In tables","OECD guidelines, comprehensive toxicity data analysis version 5.0, Dunnett's test","","7.04-8.0 on Day 0, 4.74-7.64 on Day 2. 22 mg/L = pH 5.48 and 44 mg/L = pH 4.74 on Day 2. But at EC50, pH around 7.5.","","","","22-24°C","99% purity FeCl2"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","198-4"]
["Scores",10,0,5,5,5,5,0,0,5,4,4,0,0,0,0,1,1,0,0,0,0,45,0.46391752577319589,""]
["Answers","96 h","","LC (although likley to really be EC)",50,"Diluent controls","Five replicates","","","Well water, hardness 100 m/L","Static","However, no values reported. Unclear how measured values were used, how measurements were done, if measurements were of total or dissolved Fe.","not stated","not stated","not stated","","Measured throughout but not reported. Stated as being >5.","100 mg/L","","","","Ferric chloride. Measurements likley to have been done on controls, but report is limited, so not stated."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","216-1"]
["Scores",10,10,5,5,5,5,0,0,5,0,0,0,4,0,0,0,0,0,0,1,0,50,0.51546391752577314,"The highest possible grade for this datum is acceptable."]
["Answers","96-h","Growth rate defined by number of fronds","EC50","","","3-6 reps","","","Standard Methods (1980)","","","","Stated","not stated","","","","","","27 oC",""]