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[null,null,null,null,"Ecotoxicity Data Quality Assessment Method V2"]
[null,null,null,null,null,"To be used to assess the quality of data used for the Australian and New Zealand Guidelines for Fresh and Marine Water Quality (including sediment) and the Australian National Environment Protection (Assessment of Site Contamination) Measure"]
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[null,null,null,null,null,null,"Developed by "]
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[null,null,null,null,null,null,"Zoe Zhang"]
[null,null,null,null,null,null,"   Dr. Michael Warne"]
[null,null,null,null,null,null,"Alison Vieritz "]
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[null,null,null,null,null,null,"Water Quality & Investigations "]
[null,null,null,null,null,"                 Department of Science,Information Technology and Innovation"]

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[null,"Click here to see \"All Result\" -->","All Result"]
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[null,null,null,null,"Freshwater "]
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[null,"Tabs contain questionaries -->           ","Freshwater/metal/nonplant",null,"Freshwater/non-metal/non-plant ",null,null,"Freshwater/metal/plant",null,"Freshwater/non-metal/plant"]
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[null,"Tabs contain score of paper and short answers to questions -->","Freshwater/metal/nonplant Worksheet",null,"Freshwater/non-metal/non-plant Worksheet",null,null,"Freshwater/metal/plant Worksheet",null,"Freshwater/non-metal/plant/ Worksheet"]
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[null,null,"Marine ",null,null,null,"Terrestrial",null,null,"Sediment "]
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[null,"Tab contain questionaries -->","Marine or estuarine/non-plant",null,"Marine or estuarine/Plant",null,null,"Terrestrial Data QA",null,"Sediment Data QA"]
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[null,"Tab Contains all scores of paper --> ","Marine or estuarine/non-Plant Worksheet",null,"Marine or estuarine/Plant_Worksheet",null,null,"Terrestrial Quality",null,"Sediment Quality"]

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["Freshwater/metal/plant","1-2",79,0.81443298969072164,"","Potamogeton ochreatus","apical stem cutting","30 d","NOEC","Shoot weight",7.5,"Macrophyte","Angiosperm","Monocot","Freshwater","22°C","Chronic","6.8-7.7","N.A.","Golder Associates (NZ) Limited (2010).  Effects of boron the growth and condition of Potamogeton ochreatus.  Report to Solid Energy New Zealand Ltd, 45 pp. ","1","NR","BA","Accept","Measured"]
["Freshwater/metal/plant","1-3",79,0.81443298969072164,"","Potamogeton ochreatus","apical stem cutting","30 d","NOEC","Node number",20,"Macrophyte","Angiosperm","Monocot","Freshwater","22°C","Chronic","6.8-7.7","N.A.","Golder Associates (NZ) Limited (2010).  Effects of boron the growth and condition of Potamogeton ochreatus.  Report to Solid Energy New Zealand Ltd, 45 pp. ","1","NR","BA","Accept","Measured"]
["Freshwater/metal/plant","1-4",84,0.865979381443299,"","Potamogeton ochreatus","apical stem cutting","30 d","NOEC","Plant condition",5,"Macrophyte","Angiosperm","Monocot","Freshwater","22°C","Chronic","6.8-7.7","N.A.","Golder Associates (NZ) Limited (2010).  Effects of boron the growth and condition of Potamogeton ochreatus.  Report to Solid Energy New Zealand Ltd, 45 pp. ","1","NR","BA","Reject","Measured","Non-ecological endpoint, assessed visually for abnormal plant condition. Subjective."]
["Freshwater/metal/plant","1-5",79,0.81443298969072164,"","Potamogeton ochreatus","apical stem cutting","30 d","IC10","Shoot growth",4.9000000000000004,"Macrophyte","Angiosperm","Monocot","Freshwater","22°C","Chronic","6.8-7.7","N.A.","Golder Associates (NZ) Limited (2010).  Effects of boron the growth and condition of Potamogeton ochreatus.  Report to Solid Energy New Zealand Ltd, 45 pp. ","1","NR","BA","Accept","Measured"]
["Freshwater/metal/plant","2-1",74,0.76288659793814428,"","Spirodella polyrrhiza","frond","10 d","NOEC","Growth",6.1,"Macrophyte","Angiosperm","Monocot","Freshwater","25°C","Chronic","5.2-5.8","N.A.","Davis SMD, K. D. Maier K.J. (2002). Toxicity of boron to the duckweed, Spirodella polyrrhiza. Chemosphere, 48, 615-620. ","2","NR","BA","Accept","Measured"]
["Freshwater/metal/plant","2-2",74,0.76288659793814428,"","Spirodella polyrrhiza","frond","10 d","NOEC","Abnormality",18.899999999999999,"Macrophyte","Angiosperm","Monocot","Freshwater","25°C","Chronic","5.2-5.8","N.A.","Davis SMD, K. D. Maier K.J. (2002). Toxicity of boron to the duckweed, Spirodella polyrrhiza. Chemosphere, 48, 615-620. ","2","NR","BA","Accept","Measured"]
["Freshwater/metal/plant","2-3",74,0.76288659793814428,"","Spirodella polyrrhiza","frond","10 d","NOEC","frond production","<3.5","Macrophyte","Angiosperm","Monocot","Freshwater","25°C","Chronic","5.2-5.8","N.A.","Davis SMD, K. D. Maier K.J. (2002). Toxicity of boron to the duckweed, Spirodella polyrrhiza. Chemosphere, 48, 615-620. ","2","NR","BA","Reject","Measured","Note NOEC reported as 0.9 mg/L, as this was the concentration of B in Control and all test concentrations were significant different to Control (lowest test conc 3.5 mg/L). Unreliable as a real NOEC."]
["Freshwater/metal/non- plant","3-1",78,0.78,"","Micropteris salmoides","embryo","11 d","LC10","Mortality",6,"Fish","Chordata","Actinopterygii","Freshwater","20°C","Chronic",7.5,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Black J, Barnum, JB, Birge, WJ (1993). An integrated assessment of the biological effects of boron to the rainbow trout. Chemosphere, 26(7), 1383-1413. ","3",204,"BA","Accept","Measured","1. Dyer 2001 re-derived LC10 values of work reported in Black 1993 (Ref 3), and in Birge and Black 1977 (ref 4). All three papers used for this QA. Note different pH media has resulted in different sensitivity to B.                                                                                                                                                                                                                                                                                               2. Hickey (2011) accepts the data from Black 1993 (ref 3) but not the rest of the data, stating that those datasets meet the criteria for chronic (≥7 days) but are ‘best considered acute and so was removed from the dataset’. Suggest we reconsider this. The 'accepted' 28d O. mykiss data used 0-4h post-fertilisation embryos through to 8d post hatch, which resulted in total 28d expsore. The 'rejected' data, used embryos immediately after fertilisation through to 4 days post hatching. Because of different lengths of time take for each sp to hatch, the total exposure periods differred from 7-28 days. However, all tests encompassed significant early life stages (devloping embryo, hatching, larval development and survival). Cassarius data is tabulated further down in this table. Note: O. mykiss data (LC10 of 0.7 mg/L) rejected due to large concentration range used (factor of 10).                                                                                                                                                             3. Rana pipiens control survival <90%, but all other controls for same papers were >90% (Dyer and Birge and Black data). OECD acceptable survival for amphibians is 80%, so although not stated as acceptability criteria, this dta was accepted.                                                                                                             .                                                                                                                   4. Kingett Mitchel (2004) state that Birge and Black’s Bufo fowleri, Rana pipiens, Icatalarus punctulatus and Carissius auratus data were not included because the data is unpublished. However, this criteria no longer applies. Also, the data is publihsed  – in Dyer et al and in Black et al 1993 (both journal articles)."]
["Freshwater/metal/non- plant","3-2",78,0.78,"","Oncorhynchus mykiss","embryo","32 d","LC10","Mortality",30,"Fish","Chordata","Actinopterygii","Freshwater","13°C","Chronic",7.4,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Black J, Barnum, JB, Birge, WJ (1993). An integrated assessment of the biological effects of boron to the rainbow trout. Chemosphere, 26(7), 1383-1413. ","3",197,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","3-3",81,0.81,"","Oncorhynchus mykiss","embryo","36 d","LC10","Mortality and teratogenesis",0.7,"Fish","Chordata","Actinopterygii","Freshwater","13.7°C","Chronic",7.7,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Black J, Barnum, JB, Birge, WJ (1993). An integrated assessment of the biological effects of boron to the rainbow trout. Chemosphere, 26(7), 1383-1413. ","3",188,"BA","Reject","Measured"]
["Freshwater/metal/non- plant","4-1",73,0.73,"","Oncorhynchus mykiss","embryo","28 d","LC10","Mortality and teratogenesis",2,"Fish","Chordata","Actinopterygii","Freshwater","13.7°C","Chronic",7.7,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",54,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","4-2",73,0.73,"","Oncorhynchus mykiss","embryo","28 d","LC10","Mortality and teratogenesis",8,"Fish","Chordata","Actinopterygii","Freshwater","14°C","Chronic",7.9,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",49,"BX","Accept","Measured"]
["Freshwater/metal/non- plant","4-3",73,0.73,"","Oncorhynchus mykiss","embryo","28 d","LC10","Mortality and teratogenesis",15,"Fish","Chordata","Actinopterygii","Freshwater","13°C","Chronic",7.8,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",191,"BX","Accept","Measured"]
["Freshwater/metal/non- plant","4-4",73,0.73,"","Ictalurus punctatus","embryo","9 d","LC10","Mortality and teratogenesis",5,"Fish","Chordata","Actinopterygii","Freshwater","25°C","Chronic",7.5,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",52,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","4-5",73,0.73,"","Ictalurus punctatus","embryo","9 d","LC20","Mortality and teratogenesis",9,"Fish","Chordata","Actinopterygii","Freshwater","24.7°C","Chronic",7.6,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",212,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","4-6",73,0.73,"","Ictalurus punctatus","embryo","9 d","LC10","Mortality and teratogenesis",33,"Fish","Chordata","Actinopterygii","Freshwater","29.4°C","Chronic",8.5,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",47,"BX","Accept","Measured"]
["Freshwater/metal/non- plant","4-7",73,0.73,"","Ictalurus punctatus","embryo","9 d","LC10","Mortality and teratogenesis",16,"Fish","Chordata","Actinopterygii","Freshwater","29.4°C","Chronic",8.1999999999999993,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",195,"BX","Accept","Measured"]
["Freshwater/metal/non- plant","4-8",73,0.73,"","Rana pipiens","embryo","7.5 d","LC10","Mortality and teratogenesis",48,"Amphibian","Chordata","Amphibia","Freshwater","25°C","Chronic",7.7,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",53,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","4-9",73,0.73,"","Rana pipiens","embryo","7.5 d","LC10","Mortality and teratogenesis",56,"Amphibian","Chordata","Amphibia","Freshwater","25°C","Chronic",7.8,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",212,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","4-10",73,0.73,"","Rana pipiens","embryo","7.5 d","LC10","Mortality and teratogenesis",18,"Amphibian","Chordata","Amphibia","Freshwater","25.3°C","Chronic",8.3000000000000007,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",46,"BX","Accept","Measured"]
["Freshwater/metal/non- plant","4-11",73,0.73,"","Rana pipiens","embryo","7.5 d","LC10","Mortality and teratogenesis",15,"Amphibian","Chordata","Amphibia","Freshwater","25.3°C","Chronic",8.4,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",203,"BX","Accept","Measured"]
["Freshwater/metal/non- plant","4-12",73,0.73,"","Anaxyrus fowleri","embryo","7.5 d","LC10","Mortality and teratogenesis",55,"Amphibian","Chordata","Amphibia","Freshwater","23.7°C","Chronic",7.6,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",57,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","4-13",73,0.73,"","Anaxyrus fowleri","embryo","7.5 d","LC10","Mortality and teratogenesis",30,"Amphibian","Chordata","Amphibia","Freshwater","23.7°C","Chronic",7.6,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",208,"BA","Accept","Measured"]
["Freshwater/metal/plant","5-1",44,0.45360824742268041,"The highest possible grade for this datum is acceptable. Also there was no acceptability criteria.","Chlorella pyrenoidosa","N.R.","96 h","NOEC","biomass (dry weight)",10,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","26°C","Chronic","N.A.","N.A.","Fernandez E, Sanchez E, Bonilla I, Mateo P and Ortega P (1984). Effect of boron on the growth and cell composition of Chlorella-pyrenoidosa. Phyton-International Journal of Experimental Botany, 44(2), 125-131. ","5","NR","BA","Reject","Nominal","Low quality paper. No statistics, nominal concentrations, no reported phys-chem, no reported replication, no acceptability criteria."]
["Freshwater/metal/plant","6-1",39,0.40206185567010311,"The highest possible grade for this datum is acceptable. Also there was no acceptability criteria.","Chlorella pyrenoidosa","N.R.","72 h","NOEC","Growth",25,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","26°C","Chronic","N.A.","N.A.","Sanchez (1982). Behaviour of Chlorella pyrenoidosa from diverse concentrations of boron in the media. Anales de Edafologia Y Agrobiologia, 41, 2357-2362. ","6","NR","BA","Reject","Nominal","Low quality paper. No statistics, no reported phys-chem, paper is in Spanish and could be scored higher if a translation was available."]
["Freshwater/metal/plant","7-1",27,0.27835051546391754,"The highest possible grade for this datum is acceptable. Also there was no acceptability criteria.","Chlorella vulgaris","N.R.","90-120 d","NOEC","Growth",5.2,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","N.A.","Chronic","N.A.","N.A.","Den Dooren de Jong (1965). Tolerance of Chlorella vulgaris for metallic and non-metallic ions. Antonte van Leeuwenhoek, 31, 301-313. ","7","NR","Na2B407.10H2O","Reject","Nominal","Very low quality paper. Methods are outdated (e.g. inoculation was based on addition of cells from agar slope to test media such that 'no green tinge was observable' and test was conducted on a windowsill at room temperature for 3-4 months."]
["Freshwater/metal/plant","8-1",43,0.44329896907216493,"No acceptability criteria stated or inferred.","Chlorella vulgaris","N.R.","4-7 d","NOEC","Growth",10,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","25°C","Chronic",6.8,"N.A.","McBride L, Chorney W and Skok J (1971). GROWTH OF CHLORELLA IN RELATION TO BORON SUPPLY. Botanical Gazette, 132(1), 10-&. ","8","NR","BA","Reject","Measured","Low quality paper. No statistics, no concentration response curve."]
["Freshwater/metal/plant","9-1",55,0.5670103092783505,"The highest possible grade for this datum is acceptable. Also there was no acceptability criteria.","Chlorella vulgaris","N.R.","24 d","NOEC","biomass (dry weight)",1,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","24°C","Chronic","N.A.","N.A.","McIlrath W, Skok, J (1958). Boron requirement of Chlorella vulgaris. Botanical Gazette, 119(4), 231-233. ","9","NR","BX","Reject","Nominal","Low quality paper due to lack of reported methods. Useful research however on the leaching of B from borosilicate glass. No reported test temperature, (although cited method has 24 deg C). Control (no B in solution) had lowest growth, due to B limitation. Therefore, authors compare B treatments to lowest B conc (0.5 mg/L) as Control. No statstics (although 'significant' effects described in text). Using SE values reported in Figure 1 for growth data (only), the cell number (x10^3) for 1mg/L is 11.5±1, while that for the 0.5 mg B/L (control) is 15 ± 0.1. Based on this, we can take the LOEC to be 1 and NOEC to be <1 mg/L for growth endpoint. Since we want to omit conversions from the guideline to enable high reliability, reject this data. No variability, statistics or text description other than 'probable toxicity' is provided for the biomass endpoint. Therefore biomass NOEC is unreliable. "]
["Freshwater/metal/plant","9-2",55,0.5670103092783505,"The highest possible grade for this datum is acceptable. Also there was no acceptability criteria.","Chlorella vulgaris","N.R.","24 d","NOEC","Growth","<1 ","Microalga","Chlorophyta","Chlorophyceae","Freshwater","24°C","Chronic","N.A.","N.A.","McIlrath W, Skok, J (1958). Boron requirement of Chlorella vulgaris. Botanical Gazette, 119(4), 231-233. ","9","NR","BX","Reject","Nominal"]
["Freshwater/metal/plant","10-1",78,0.80412371134020622,"Based on nominal concs, but measured were within 95%","Pseudokirchneriella subcapitata","exponentially growing cells","72 h","NOEC","Growth (yield)",17,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","23°C","Chronic","7.5-8.3","N.A.","Hanstveit A, Oldersma, H. (2000). Determination of the effect of boric acid, manufacturing grade on the growth of the freshwater green alga Pseudokirchneriella subcapitata (OECD guideline No 201 and EU C.3). TNO Nutrition and Food REsearch Institute, Netherlands. ","10",24,"BA","Accept","Based on nominal, but measured were wtihin 95% of nominal","1. These values differ from that in the previous B GV (132 mg/L). Unclear how value of 132 mg/L achieved. Perhaps preivously an average was taken of the NEC 157, NOEC 100 and NOEC (biomass) 140, you get 132. However these values are reported for boric acid, not B. Therefore, use values for B reported here in this document which have been converted to mg//L B.                                                                                      2. An EC10 of 140 mg/L was also calcualted for biomass, however there is uncertainlty in that value since the report states that statistical significance was only found for the growth endpoint, not the biomass endpoint.                                                         "]
["Freshwater/metal/plant","10-2",83,0.85567010309278346,"Based on nominal concs, but measured were within 95%","Pseudokirchneriella subcapitata","exponentially growing cells","72 h","EC10","Growth (yield)",35,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","23°C","Chronic","7.5-8.3","N.A.","Hanstveit A, Oldersma, H. (2000). Determination of the effect of boric acid, manufacturing grade on the growth of the freshwater green alga Pseudokirchneriella subcapitata (OECD guideline No 201 and EU C.3). TNO Nutrition and Food REsearch Institute, Netherlands. ","10",24,"BA","Accept","Based on nominal, but measured were wtihin 95% of nominal"]
["Freshwater/metal/plant","10-3",82,0.84536082474226804,"Based on nominal concs, but measured were within 95%","Pseudokirchneriella subcapitata","exponentially growing cells","72 h","NEC","Growth (yield)",27,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","23°C","Chronic","7.5-8.3","N.A.","Hanstveit A, Oldersma, H. (2000). Determination of the effect of boric acid, manufacturing grade on the growth of the freshwater green alga Pseudokirchneriella subcapitata (OECD guideline No 201 and EU C.3). TNO Nutrition and Food REsearch Institute, Netherlands. ","10",24,"BA","Accept","Based on nominal, but measured were wtihin 95% of nominal"]
["Freshwater/metal/non- plant","11-1",85,0.85,"","Danio rerio","embryo","34 d","NOEC","Mortality",5.6,"Fish","Chordata","Actinopterygii","Freshwater","25 ± 1°C","Chronic","7.2-8.0","N.A.","Hooftman RN, van Drongelen-Sevenhuijsen, D., de Haan, H.P.M. (2000). Early life stage test under semi-static conditions with boric acid, manufacturing grade and the zebrafish Brachydanio rerio. TNO Report V99.168, TNO Nutrition and Food, REsearch Institute Netherlands. ","11",212,"BA","Accept","Measured","Name change - Danio rerio is currently accepted name for Bracydanio rerio"]
["Freshwater/metal/non- plant","11-2",85,0.85,"","Danio rerio","embryo","34 d","NOEC","Biomass (length)",5.6,"Fish","Chordata","Actinopterygii","Freshwater","25 ± 1°C","Chronic","7.2-8.0","N.A.","Hooftman RN, van Drongelen-Sevenhuijsen, D., de Haan, H.P.M. (2000). Early life stage test under semi-static conditions with boric acid, manufacturing grade and the zebrafish Brachydanio rerio. TNO Report V99.168, TNO Nutrition and Food, REsearch Institute Netherlands. ","11",212,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","11-3",85,0.85,"","Danio rerio","embryo","34 d","NOEC","Biomass (dry weight)",1.8,"Fish","Chordata","Actinopterygii","Freshwater","25 ± 1°C","Chronic","7.2-8.0","N.A.","Hooftman RN, van Drongelen-Sevenhuijsen, D., de Haan, H.P.M. (2000). Early life stage test under semi-static conditions with boric acid, manufacturing grade and the zebrafish Brachydanio rerio. TNO Report V99.168, TNO Nutrition and Food, REsearch Institute Netherlands. ","11",212,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","12-1",57,0.56999999999999995,"Note - if original report was available, this QA will be higher, as study done alognside 10 and 11.","Daphnia magna","neonates","21 d","NOEC","Reproduction",10,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","19.3-20.9°C","Chronic","7.2-8.0","N.A.","Hooftman R.N., van Drongelen-Sevenhuijsen  D., de Haan H.P.M., 2000c, Semi-static reproduction test with Boric Acid, Manufacturing Grade and Daphnia magna. TNO Study 99-9047-10. Report V99-169. TNO Nutrition and Food Research Institute, Delft, The Netherlands, cited in Austria (2008). TRANSITIONAL ANNEX XV DOSSIER, Boric acid. \nHooftman R.N., van Drongelen-Sevenhuijsen D., de Haan H.P.M., 2000c, Semi-static reproduction\ntest with Boric Acid, Manufacturing Grade and Daphnia magna. TNO Study 99-9047-10. Report V99-169. TNO Nutrition and Food Research Institute, Delft, The Netherlands. Reported in Austria 2008 Transitional Annex XV Dossier, Boric Acid.\n\n","12",212,"BA","Accept","Measured","Original paper was not available. Data was taken from Austria Reach document. Assume high quality since study done alongside references 10 and 11 above, which scored high."]
["Freshwater/metal/non- plant","13-1",88,0.88,"","Daphnia magna","neonates","21 d","NOEC","Reproduction (number of young)",6.4,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","19.5-20.5°C","Chronic","8.1 ± 0.1 in Control","N.A.","Gersich FM (1984). Evaluation of a static renewal chronic toxicity test method for daphnia-magna straus using boric-acid. Environmental Toxicology and Chemistry, 3(1), 89-94. ","13",148,"BA","Accept","Measured","Only Gerisch 1984 quoted, since Gerisch 1985 reports identical dataset."]
["Freshwater/metal/non- plant","13-2",88,0.88,"","Daphnia magna","neonates","21 d","NOEC","Reproduction (number of broods)",6.4,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","19.5-20.5°C","Chronic","8.1 ± 0.1 in Control","N.A.","Gersich FM (1984). Evaluation of a static renewal chronic toxicity test method for daphnia-magna straus using boric-acid. Environmental Toxicology and Chemistry, 3(1), 89-94. ","13",148,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","13-3",88,0.88,"","Daphnia magna","neonates","21 d","NOEC","Reproduction (mean brood size/daphnid)",6.4,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","19.5-20.5°C","Chronic","8.1 ± 0.1 in Control","N.A.","Gersich FM (1984). Evaluation of a static renewal chronic toxicity test method for daphnia-magna straus using boric-acid. Environmental Toxicology and Chemistry, 3(1), 89-94. ","13",148,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","13-4",83,0.83,"","Daphnia magna","neonates","21 d","NOEC","Mortality",29,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","19.5-20.5°C","Chronic","8.1 ± 0.1 in Control","N.A.","Gersich FM (1984). Evaluation of a static renewal chronic toxicity test method for daphnia-magna straus using boric-acid. Environmental Toxicology and Chemistry, 3(1), 89-94. ","13",148,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","13-5",83,0.83,"","Daphnia magna","neonates","21 d","NOEC","Biomass (length)",6.4,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","19.5-20.5°C","Chronic","8.1 ± 0.1 in Control","N.A.","Gersich FM (1984). Evaluation of a static renewal chronic toxicity test method for daphnia-magna straus using boric-acid. Environmental Toxicology and Chemistry, 3(1), 89-94. ","13",148,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","14-1",85,0.85,"","Daphnia magna","neonates","14 d","NOEC","Reproduction ( number of young)",2.4,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","24°C","Chronic","7.3-8.2","N.A.","Gersich FM, Milazzo, D.P. (1990). Evaluation of a 14-day static renewal toxicity test with Daphnia magna Straus. Archives of Environmental Contamination and Toxicology, 19, 72-76. ","14",170,"BA","Accept","Measured","Values converted from H3BO3 concentrations to B."]
["Freshwater/metal/non- plant","14-2",85,0.85,"","Daphnia magna","neonates","14 d","NOEC","Reproduction (brood size)",2.4,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","24°C","Chronic","7.3-8.2","N.A.","Gersich FM, Milazzo, D.P. (1990). Evaluation of a 14-day static renewal toxicity test with Daphnia magna Straus. Archives of Environmental Contamination and Toxicology, 19, 72-76. ","14",170,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","14-3",80,0.8,"","Daphnia magna","neonates","14 d","NOEC","Mortality",2.4,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","24°C","Chronic","7.3-8.2","N.A.","Gersich FM, Milazzo, D.P. (1990). Evaluation of a 14-day static renewal toxicity test with Daphnia magna Straus. Archives of Environmental Contamination and Toxicology, 19, 72-76. ","14",170,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","14-4",80,0.8,"","Daphnia magna","neonates","14 d","NOEC","Biomass (dry weight)",2.4,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","24°C","Chronic","7.3-8.2","N.A.","Gersich FM, Milazzo, D.P. (1990). Evaluation of a 14-day static renewal toxicity test with Daphnia magna Straus. Archives of Environmental Contamination and Toxicology, 19, 72-76. ","14",170,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","14-5",85,0.85,"","Daphnia magna","neonates","14 d","NOEC","Reproduction ( number of young)",2.5,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","24°C","Chronic","7.3-8.2","N.A.","Gersich FM, Milazzo, D.P. (1990). Evaluation of a 14-day static renewal toxicity test with Daphnia magna Straus. Archives of Environmental Contamination and Toxicology, 19, 72-76. ","14",170,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","14-6",85,0.85,"","Daphnia magna","neonates","14 d","NOEC","Reproduction (brood size)",2.5,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","24°C","Chronic","7.3-8.2","N.A.","Gersich FM, Milazzo, D.P. (1990). Evaluation of a 14-day static renewal toxicity test with Daphnia magna Straus. Archives of Environmental Contamination and Toxicology, 19, 72-76. ","14",170,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","14-7",80,0.8,"","Daphnia magna","neonates","14 d","NOEC","Mortality",2.5,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","24°C","Chronic","7.3-8.2","N.A.","Gersich FM, Milazzo, D.P. (1990). Evaluation of a 14-day static renewal toxicity test with Daphnia magna Straus. Archives of Environmental Contamination and Toxicology, 19, 72-76. ","14",170,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","14-8",80,0.8,"","Daphnia magna","neonates","14 d","NOEC","Biomass (dry weight)",2.5,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","24°C","Chronic","7.3-8.2","N.A.","Gersich FM, Milazzo, D.P. (1990). Evaluation of a 14-day static renewal toxicity test with Daphnia magna Straus. Archives of Environmental Contamination and Toxicology, 19, 72-76. ","14",170,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","15-1",72,0.72,"The highest possible grade for this datum is acceptable.","Daphnia magna","neonates","14 d","NOEC","Reproduction ( number of young)","18  nominal","Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","20°C","Chronic",7.9,"N.A.","Hickey CW (1989). Sensitivity of 4 New-Zealand cladoceran species and Daphnia-magna to aquatic toxicants. New Zealand Journal of Marine and Freshwater Research, 23(1), 131-137. ","15",250,"BA","Accept","Nominal","This data is nominal. Article states \"chemical anlaysis of water concentrations of toxicants was not tested\". "]
["Freshwater/metal/non- plant","15-2",72,0.72,"The highest possible grade for this datum is acceptable.","Ceriodaphnia dubia","neonates","14 d","NOEC","Reproduction ( number of young)","10  nominal","Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","20°C","Chronic",7.9,"N.A.","Hickey CW (1989). Sensitivity of 4 New-Zealand cladoceran species and Daphnia-magna to aquatic toxicants. New Zealand Journal of Marine and Freshwater Research, 23(1), 131-137. ","15",250,"BA","Accept","Nominal"]
["Freshwater/metal/non- plant","16-1",87,0.87,"","Daphnia magna","neonates","21 d","NOEC","Reproduction ( number of young)",6,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","19°C","Chronic","7.1-8.7","N.A.","Lewis MA and Valentine LC (1981). Acute and chronic toxicities of boric-acid to Daphnia-magna Straus. Bulletin of Environmental Contamination and Toxicology, 27(3), 309-315. ","16",166,"BA","Accept","Measured","Dilution water incorrect on previous guideline document. Amended in current document."]
["Freshwater/metal/non- plant","16-2",87,0.87,"","Daphnia magna","neonates","21 d","NOEC","Biomass (length)",27,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","19°C","Chronic","7.1-8.7","N.A.","Lewis MA and Valentine LC (1981). Acute and chronic toxicities of boric-acid to Daphnia-magna Straus. Bulletin of Environmental Contamination and Toxicology, 27(3), 309-315. ","16",166,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","17-1",58,0.57999999999999996,"Note: no acceptability criteria stated","Oncorhynchus mykiss","embryo","42 d","NOAEL","Mortality",86,"Fish","Chordata","Actinopterygii","Freshwater","12.5°C","Chronic","N.A.","N.A.","Rowe RI, Bouzan C, Nabili S and Eckhert CD (1998). The response of trout and zebrafish embryos to low and high boron concentrations is U-shaped. Biological Trace Element Research, 66(1-3), 261-270. ","17","NR","BA","Accept","Measured","LOAEL reported as 10 mmol B/L. Test concentrations were 0, 2, 4, 8, 10 mmol. Therefore NOEC taken to be 8. Convert 8 mmol to 86 mg/L. Note also - previous guideline document also included Danio rerio data but this is considered acute (4d) so not included in this assessment."]
["Freshwater/metal/plant","18-1",78,0.80412371134020622,"Note: no acceptability criteria stated","Egeria densa","apical stem cutting","28 d","NOEC","biomass (dry weight)",6.1,"Macrophyte","Angiosperm","Monocot","Freshwater","18.5-21.4°C","Chronic","7.7-10.1","N.A.","Thompson (1987). Studies of the toxicity of boron and Huntley West Mine waste waters to the aquatic macrophyte Egeria densa. Technical Report, University of Waikato, Water Research Unit, 65 pp. ","18",23,null,"Accept","Measured","NOEC of 6.1 is taken from measured concentrations of test treatment solutions in Table 10 at end of report. Hickey and Macasgill report this as NOEC of 5, LOEC 10. Hickey (2011) uses MATC/2 = 3.5 mg/L. Note statistics done differently to current day approaches."]
["Freshwater/metal/non- plant","19-1",68,0.68,"The highest possible grade for this datum is acceptable. Also no acceptability criteria reported","Oncorhynchus kisutch","alevin","11.8 d","LC50","Mortality",113,"Fish","Chordata","Actinopterygii","Freshwater","11 ± 0.5°C","Chronic","N.A.","N.A.","Thompson J, Davis, JC, Drew, RE (1976). Toxicity, uptake and survey studies of boron in the marine environment. Water Research, 10, 869-875. ","19",47,"Na2B2O4.8H2O","Accept, however, only use if NOEC or EC10 not available, or if insufficient NOECs and EC10s for GV derivation as per Warne et al, 2018.","Nominal. Note B was measured in effluent and tissue, not test waters.","Not in previous B GV, but it is in Hickey (2011) and Kinget Mithcell (2004). This test was conducted for 12d, at 11 degrees C, outside the 'temperate' range for which the table of reccomended acute and chronic test exposure lengths are (Warne et al, 2018). Therefore, it is not known if 12d for this species consititues chronic exposure. Additionally, while this paper achieved a pass score, no acceptability criteria are stated, and control responses are not stated. Finally, Warne et al, 2018 states that converted chronic LC50s and LOECs to NOECs are to be used if there are insufficent preferred values (e.g. NECs, EC10s, NOECs). Therefore only use this data if there are insufficient EC10s/NOECs for GV derivation."]
["Freshwater/metal/non- plant","20-1",91,0.91,"","Lampsilis siliquoidea","juvenile","21 d","NOEC","Biomass (length)",10,"Bivalve","Mollusca","Bivalve","Freshwater","20°C","Chronic","6.8-7.9","N.A.","Hall S, Lockwood R and Harrass MC (2014). Application of a Unique Test Design to Determine the Chronic Toxicity of Boron to the Aquatic Worm Lumbriculus variegatus and Fatmucket Mussel Lampsilis siliquoidea. Archives of Environmental Contamination and Toxicology, 66(1), 58-68. ","20","89-108","BA","Accept","Measured","Discrepancy in article between text (LOECs) and Table values. Suspect text is correct and table has typo, based on the numbers themselves and where the effect appears to be significant from dose-response. Therefore have used the text-described LOEC of 17.8 mg/L and NOEC of 10 mg/L for growth endpoint. Survival endpoint in text and table matches."]
["Freshwater/metal/non- plant","20-2",87,0.87,"","Lampsilis siliquoidea","juvenile","21 d","IC25","Biomass (length)",25,"Bivalve","Mollusca","Bivalve","Freshwater","20°C","Chronic","6.8-7.9","N.A.","Hall S, Lockwood R and Harrass MC (2014). Application of a Unique Test Design to Determine the Chronic Toxicity of Boron to the Aquatic Worm Lumbriculus variegatus and Fatmucket Mussel Lampsilis siliquoidea. Archives of Environmental Contamination and Toxicology, 66(1), 58-68. ","20","89-108","BA","Accept","Measured"]
["Freshwater/metal/non- plant","20-3",86,0.86,"","Lampsilis siliquoidea","juvenile","21 d","NOEC","Mortality",32,"Bivalve","Mollusca","Bivalve","Freshwater","20°C","Chronic","6.8-7.9","N.A.","Hall S, Lockwood R and Harrass MC (2014). Application of a Unique Test Design to Determine the Chronic Toxicity of Boron to the Aquatic Worm Lumbriculus variegatus and Fatmucket Mussel Lampsilis siliquoidea. Archives of Environmental Contamination and Toxicology, 66(1), 58-68. ","20","89-108","BA","Accept","Measured"]
["Freshwater/metal/non- plant","20-4",82,0.82,"","Lampsilis siliquoidea","juvenile","21 d","IC25","Mortality",38,"Bivalve","Mollusca","Bivalve","Freshwater","20°C","Chronic","6.8-7.9","N.A.","Hall S, Lockwood R and Harrass MC (2014). Application of a Unique Test Design to Determine the Chronic Toxicity of Boron to the Aquatic Worm Lumbriculus variegatus and Fatmucket Mussel Lampsilis siliquoidea. Archives of Environmental Contamination and Toxicology, 66(1), 58-68. ","20","89-108","BA","Accept","Measured"]
["Freshwater/metal/plant","21-1",44,0.45360824742268041,"The highest possible grade for this datum is acceptable.","Chara corallina","N.R.","7 d","NOEC","Growth","<11 ","Macrophyte","Charophyta","Charophyceae","Freshwater","N.A.","Chronic",6,"N.A.","Reid R, Hayes, JE, Post, A., Stangoulis, JCR, Graham, RD (2004). A critical anlaysis of the causes of boron toxicity in plants. Plant, Cell and Environment, 25, 1405-1414. ","21",null,"BA","Reject","Nominal","Low quality paper. No specified replication, statistics or phys-chem data apart from Control pH."]
["Freshwater/metal/non- plant","22-1",76,0.76,"","Hyalella azteca","juvenile","42 d","NOEC","Mortality",26,"Macro-invertebrate","Arthropoda","Malacostraca","Freshwater","22°C","Chronic",8.1,"N.A.","Soucek DJ, Dickinson A and Koch BT (2011). ACUTE AND CHRONIC TOXICITY OF BORON TO A VARIETY OF FRESHWATER ORGANISMS. Environmental Toxicology and Chemistry, 30(8), 1906-1914. ","22",106,"BA and BX","Accept","Measured","New data"]
["Freshwater/metal/non- plant","22-2",81,0.81,"","Hyalella azteca","juvenile","42 d","NOEC","Reproduction ( number of young)",6.6,"Macro-invertebrate","Arthropoda","Malacostraca","Freshwater","22°C","Chronic",8.1,"N.A.","Soucek DJ, Dickinson A and Koch BT (2011). ACUTE AND CHRONIC TOXICITY OF BORON TO A VARIETY OF FRESHWATER ORGANISMS. Environmental Toxicology and Chemistry, 30(8), 1906-1914. ","22",106,"BA and BX","Accept","Measured","New data"]
["Freshwater/metal/non- plant","22-3",79,0.79,"","Pimephales promelas","embryo","32 d","NOEC","Mortality",11,"Fish","Chordata","Actinopterygii","Freshwater","25°C","Chronic",8,"N.A.","Soucek DJ, Dickinson A and Koch BT (2011). ACUTE AND CHRONIC TOXICITY OF BORON TO A VARIETY OF FRESHWATER ORGANISMS. Environmental Toxicology and Chemistry, 30(8), 1906-1914. ","22",106,"BA and BX","Accept","Measured","New data"]
["Freshwater/metal/plant","23-1",67,0.69072164948453607,"The highest possible grade for this datum is acceptable. Also no acceptability criteria reported","Chlorella pyrenoidosa","late log phase","14 d","NOEC","Growth (rate)",0.4,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","25°C","Chronic","N.A.","N.A.","Wong PK and Wong CK (1990). TOXICITY OF NICKEL AND NICKEL ELECTROPLATING WATER TO CHLORELLA-PYRENOIDOSA. Bulletin of Environmental Contamination and Toxicology, 45(5), 752-759. ","23","NR","BA","Reject","Nominal","Statistics are done correctly, conc-response curve is evident. Tests were run in 'modified complete medium' recipe for which is not given but a thesis is referred to for it. I found a copy of the thesis online (http://repository.lib.cuhk.edu.hk/en/item/cuhk-321132?solr_nav%5Bid%5D=6f52e86a94b4c09ab265&solr_nav%5Bpage%5D=0&solr_nav%5Boffset%5D=0). Recipe does not contain boron, but does contain EDTA. Main justification for rejection, however, is that algal growth was determined using absorbance at 650 nm, not an accepated or reliable proxy for algal cell density."]
["Freshwater/metal/non- plant","24-1",85,0.85,"","Pimephales promelas","embryo","32 d","NOEC","Mortality",13,"Fish","Chordata","Actinopterygii","Freshwater","25 ± 1°C","Chronic","8.1-8.3","N.A.","USEPA (2010). Final Report on Acute and Chornic toxicity of nitrate, nitrite, boron, manganese, fluoride, chloride and sulfate to several aquatic animal species. ","24","112-132","BA","Accept","Measured","Note: 2 of 4 controls crashed part way through the test, but this was explained by mishandling of the fish in those treatments. Therefore, only 2 of four controls were used in statistical comparisons. There is good level of detail int his report, and it scored high, therefore accept this data."]
["Freshwater/metal/non- plant","24-2",85,0.85,"","Pimephales promelas","embryo","32 d","LC10","Mortality",13,"Fish","Chordata","Actinopterygii","Freshwater","25 ± 1°C","Chronic","8.1-8.3","N.A.","USEPA (2010). Final Report on Acute and Chornic toxicity of nitrate, nitrite, boron, manganese, fluoride, chloride and sulfate to several aquatic animal species. ","24","112-132","BA","Accept","Measured"]
["Freshwater/metal/non- plant","24-3",85,0.85,"","Pimephales promelas","embryo","32 d","NOEC","Biomass (dry weight)","<5.9 ","Fish","Chordata","Actinopterygii","Freshwater","25 ± 1°C","Chronic","8.1-8.3","N.A.","USEPA (2010). Final Report on Acute and Chornic toxicity of nitrate, nitrite, boron, manganese, fluoride, chloride and sulfate to several aquatic animal species. ","24","112-132","BA","Accept","Measured"]
["Freshwater/metal/non- plant","24-4",85,0.85,"","Pimephales promelas","embryo","32 d","EC10","Biomass (dry weight)",22,"Fish","Chordata","Actinopterygii","Freshwater","25 ± 1°C","Chronic","8.1-8.3","N.A.","USEPA (2010). Final Report on Acute and Chornic toxicity of nitrate, nitrite, boron, manganese, fluoride, chloride and sulfate to several aquatic animal species. ","24","112-132","BA","Accept","Measured"]
["Freshwater/metal/non- plant","4-14",77,0.77,"","Carassius auratus","embryo","7 d","LC10","Mortality",16,"Fish","Chordata","Actinopterygii","Freshwater","24.8 ± 0.3°C","Chronic",7.9,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",54,"BA","Accept","Measured","See comments above for Reference number 4."]
["Freshwater/metal/non- plant","4-15",77,0.77,"","Carassius auratus","embryo","7 d","LC10","Mortality",15,"Fish","Chordata","Actinopterygii","Freshwater","24.8 ± 0.3°C","Chronic",7.6,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",208,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","4-16",77,0.77,"","Carassius auratus","embryo","7 d","LC10","Mortality",20,"Fish","Chordata","Actinopterygii","Freshwater","27 ± 0.4°C","Chronic",8.3000000000000007,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",46,"BX","Accept","Measured"]
["Freshwater/metal/non- plant","4-17",77,0.77,"","Carassius auratus","embryo","7 d","LC10","Mortality",16,"Fish","Chordata","Actinopterygii","Freshwater","27 ± 0.4°C","Chronic",8.1,"N.A.","Dyer SD (2001). Determination of the aquatic PNEC0.05 for boron. Chemosphere, 44(3), 369-376. AND Birge WJ BJ (1977). Sensitivity of vertebrate embryos to boron compounds. EPA report EPA-560/1-76-008 Final Report. ","4",195,"BX","Accept","Measured"]
["Freshwater/metal/plant","25-1",49,0.50515463917525771,"The highest possible grade for this datum is acceptable. Also no acceptability criteria reported","Anacystis nidulans","N.R.","96 h","NOEC","Growth",50,"Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","26°C","Chronic","N.A.","N.A.","Martinez F, Mateo, P., Bonilla I., Fernandez-Valiente, E. (1986). Cellular changes to boron toxicity in the blue-green alga Anacystis nidulans. Oyton, 46(2), 145-152. ","25","NR","BA","Reject","Nominal. Report staes B was measured, but no reported concentrations","Low quality paper. Data is nominal. MDS statstics done to show signficance of results, but no stats to show which result is significantly different from which. i.e. NOEC unreliable."]
["Freshwater/metal/plant","26-1",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Melosira granulata (currently accepted name: Aulacoseira granulata)","N.R.","10-20d","NOEC","Growth rate (Chl a)",3,"Microalga","Bacillariophyta","Bacillariophyceae ","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject. Dilution water (Lake water) had elevated B (0.47 mg/L) and history of elevated As (Hickey pers comm) ","Nominal. Report staes B was measured, but no reported concentrations","Wilkinson 1985 report:\nThis dataset has been discussed at length amongst authors of this B DGV document. Below is a summary of agreed justifications for use or rejection of each toxicity value, as well as limitations of the data. \n\nHistory: \nThe data was included in previous guideline document, however incorrect values were used (see below). Kingett Mitchell (2004) rejected the data when re-deriving B guideline value. Dr. Chris Hickey accepted the data when reviewing and updating freshwater boron GVs following the ANZECC/ARMCANZ (2000) approach (Hickey, 2011).\n\nLimitations/justifications:\nExposure lengths: Exposure period is not possible to define for any toxicity value in this dataset. Tests were conducted for between 8 and 36 days, depending on species, but the endpoint used for statistically derived NOECs were maximum growth rate and maximum yield – whenever these occurred over the whole exposure period, so for example for Navicula sp 1, Control maximum yield occurred on Day 6, while for all B treatments it was on Day 8 or 10, so the yield values for control Day 6 are compared to those for B treatments on other days to derive NOEC. Inhibition of lag phase was also assessed but these data were not statistically compared to Controls. For all species, maximum growth rates and yield were achieved at least by 96h. \nIt was agreed that comparison of growth data from different timepoints is probably acceptable (and was suited to the aims of the study in question), but may underestimate toxicity – as it doesn’t take into account time. i.e. algae in low concentrations of B can achieve the same max growth rate or yield eventually as the Control but they take longer to do so, which implies a toxic effect that is not captured using the endpoints in this report. The lag-phase endpoint tries to do this to some extent, but this ordinal data (measured in days – Day 2, 4 etc), was not statistically analysed (although a definition of lag phase toxicity is given). \n\nGrowth rate v yield: Where possible, maximum growth rates were preferred to yield values, as the yield values relate to the ability for ecological dominance in the lake, and often yield values for very different time points are compared. Use of yield values were considered on a case-by-case basis for each species if growth rate data were unreliable.    \n                                                                                                                                                                                                                                                                                                                     Chl a: OECD guideline 201 and USEPA methods (e.g. USEPA, 2002) both state that chlorophyll fluorescence (or similar surrogate) can be used in place of direct cell counting methods if a satisfactory correlation with biomass can be demonstrated over the range of biomass occurring in the test. Dr. Jenny Stauber (Jenny Stauber, CSIRO, Australia, pers. comm.) concurred with this – saying that they should be reporting a calibration of some sort between biomass and Chl a, and they also need to show that this doesn’t change over the densities encountered in the testing period. However there is no evidence of this in the report. From our own algal work, we know that effects on the pigments in cells (Chl a) doesn't always translate to effects on biomass. While there is no direct calibration in the Wilkinson report, Wilkinson did run multi-algal tests in which direct microscope algal counts were used to determine maximum growth rates. These were compared to those growth rates achieved in the unialgal experiments in response to B, and gives some confidence in the method. However, this does not strictly serve as a calibration between the Chl a and direct counting methods, since it is not known if any difference between the growth rate of the alga when tested alone and in combination with another alga is due to the presence of the additional alga or to the different methods used to determine biomass. After discussion amongst co-authors Monique Binet, Chris Hickey and Graeme Batley, based on the use of Chl a widely as a proxy for cell density, it has been agreed that Chl a –based endpoints in this study are acceptable.\n\nOther points:                                                                                                                                                                                                                                                                                      1. Nominal values used. No reported measured B concentrations (other than in dilution water for Melosira tests - 0.47 mg/L), even though it states B was measured beginning and end of test. It is likely that the lack of reported B concentrations indicates confirmatory analysis and the use of apparent nominal concentrations in analysis would be taken as achieving target concentrations in experiment. Regardless, since there is no mention of the actual measured values, this data has been listed as nominal.                                                                                                                                                                 \n2. No acceptability criteria stated or inferred, however, growth curves provided that show favourable control growth. Those species where Control growth was suppressed compared to B treatments (i.e. where essentiality of B was likely) were not included.                                                                                                                                     \n3. High initial cell density (1x105 cells/mL), therefore toxicity may be underestimated compared to more environmentally relevant densities.                                                                                                                                         \n4. Wilkinson also mentions 'final yield' occasionally - but the method used to calculate this is not reported, nor are the values. In addition, Wilkinson states that ‘final yields’ are not strictly comparable because the initial concentration of the innoculum was markedly different for different species.                                                                                                                                                                                                                                                                                                                                                                                          \n5. Data values from this report used in previous ANZECC GV were amended - values in previous guideline document appear to have 0.47 mg/L added to all toxicity values. It is possible this was done because there was 0.47 mg B/L in the test media for one algal species Melosira (tested in Lake water), however, the dilution water for all other test species (1/10th diluted Woods Hole MBL Media to match the N and P to that in Waikato River) did not contain B. Also, this approach (correcting NOEC values for B in media) has not been done for other data.                                                                                                                                                                                   \n6. Kingett Mitchel (2004) classified the data as unacceptable for use for moderate or high reliability guideline value. They were concerned about nominal values, and of the use of borisilicate glass in tests which are known to leach boron. Wilkinson states that 0.1-1.0 mg/L leaches from the glassware during autoclaving. Some other studies take into account this leaching, but not all. If we consider this point (use of borosilicate glass) crucial, we must also re-check all other reported nominal data for whether the borosilicate glass leaching was accounted for, for consistency.\n7. Navicula sp 1 and 2 are two separate species, and should not be grouped. \n\nReferences stated here but not elsewhere in this file: \nHickey, C. (2011). Review of freshwater boron guidelines following ANZECC 2000 procedure. Memorandum to Graeme Batley, Chair WQ4, July 2011, Ref MFE11201, NIWA, New Zealand.\nKingett Mitchell (2004). Recalculation of the ANZECC water quality criterion for boron. Appendix 9, October 2004, Kingett Mithcel Ltd, Auckland, New Zealand.\nOECD (2011). Guideline 201. Freshwater alga and cyanobacteria, growth inhibition test. OECD guidelines for the testing of chemicals, updated 28 July 2011. Organisation for Economic Co-operation and Development, Paris.\nUSEPA (2002). Method 1003.0: Green Alga, Selenastrum capricornutum, Growth Test; Chronic Toxicity. Excerpt from: Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms 4th edition (2002), EPA-821-R-02-013. United States Environmental Protection Authority, Office of Water, USA.\n\n"]
["Freshwater/metal/plant","26-2",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Melosira granulata (currently accepted name: Aulacoseira granulata)","N.R.","10-20d","NOEC","Growth, yield (Chl a)",3,"Microalga","Bacillariophyta","Bacillariophyceae ","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject. Dilution water (Lake water) had elevated B (0.47 mg/L) and history of elevated As (Hickey pers comm) ","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-3",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Cyclotella sp","N.R.","4-14d","NOEC","Growth rate (Chl a)",">30 ","Microalga","Bacillariophyta","Mediophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject >value. Unreliable.","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-4",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Cyclotella sp","N.R.","4-14d","NOEC","Growth, yield (Chl a)",10,"Microalga","Bacillariophyta","Mediophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Growth endpoint preferable, however, based on assessment of growth curves, yield acceptable for this species. New IC10 not calculatable from raw data.","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-5",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Navicula sp 1.","N.R.","4-12d","NOEC","Growth rate (Chl a)","<1 ","Microalga","Bacillariophyta","Bacillariophyceae ","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Accept, used re-calculated IC10 of 0.6 mg/L for SSD, using reported mean values for each treatment, as clear monotonic conc-response curve evident.","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-6",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Navicula sp 1.","N.R.","4-12d","NOEC","Growth, yield (Chl a)",3,"Microalga","Bacillariophyta","Bacillariophyceae ","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject, yield values deemed not appropriate for this dataset, see notes in column z.","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-7",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Navicula sp. 2","N.R.","4-16d","NOEC","Growth rate (Chl a)",">30 ","Microalga","Bacillariophyta","Bacillariophyceae ","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Accept","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-8",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Navicula sp. 2","N.R.","4-16d","NOEC","Growth, yield (Chl a)",1,"Microalga","Bacillariophyta","Bacillariophyceae ","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Growth endpoint preferable, however, based on assessment of growth curves, yield acceptable for this species.","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-9",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Synedra sp.","N.R.","4-18d","NOEC","Growth rate (Chl a)","<1 ","Microalga","Bacillariophyta","Bacillariophyceae ","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject.Could use, but would need to use LOEC/2.5 conversion. Cannot re-calcualte IC10 value due to interrupted dose-response curve. ","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-10",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Synedra sp.","N.R.","4-18d","NOEC","Growth, yield (Chl a)","<1 ","Microalga","Bacillariophyta","Bacillariophyceae ","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject, yield values deemed not appropriate for this dataset. Interrupted dose-response, so EC10 cannot be re-calculated.","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-11",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Anabaena oscillarioides 1","N.R.","4-8d","NOEC","Growth rate (Chl a)",10,"Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject - bloom forming/nuisance and/or toxin producing blue-green algal species","Nominal. Report staes B was measured, but no reported concentrations","Use of Wilkinson (1985) Blue-green algal data:\n\nThe aim of the Wilkinson (1985) report was to determine if blue-green algal species would dominate the phytoplankton communities of the Waikito River if B concentrations increased as result of added discharge from a geothermal power station.  \n\nOf the blue-green algal species in this study, Nostoc punctiforme is the only species that is not linked with toxin production or blooms. This nitrogen-fixing species has strong symbiotic relationships with plants and fungi. Therefore data for this species has been accepted. \n\nTwo strains were used in the study, N. punctiforme 1 (isolated 'some years' ago and cultured in Anabaena standard medium which contains 0.12 mg/L added B) and N. punctiforme 2 (isolated specifically for this study from Lake Ohakuri, cultured in boron-free 0.1 strength Woods Hole medium). Therefore only data for N. punctiforme 2 was accepted. \n\nThe NOEC value for N. punctiforme 2 growth rate is <1 mg/L, because all tested concentrations of B (1, 3, 10, 30 mg/L) caused toxicity. The growth rate response data for this species is a flat level response across all treatments for growth rate (63, 74, 79, 65% control for respective B concentrations of 1, 3, 10, 30 mg/L). This is likely because of the way the growth rate is calculated - not at a particular time-point, but wherever it was at maximum for each test treatment. Assessment of the growth curves for this data, the control, 1, 3 and 10 mg/l b treatments overlap and have similar growth patterns at each time point. The 30 mg/l treatment is vastly different. For this dataset, the maximum yield endpoint is a suitable measure of toxicity. Maximum yield for all treatments occur at a similar time and reflects the difference in growth curves of 30 mg/L B treatment to the other treatments. The yeild value of 10 mg/L was deemed reliable and acceptable.  This value used in SSD."]
["Freshwater/metal/plant","26-12",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Anabaena oscillarioides 1","N.R.","4-8d","NOEC","Growth, yield (Chl a)",1,"Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject - bloom forming/nuisance and/or toxin producing blue-green algal species","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-13",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Anabaena oscillarioides 2","N.R.","8-36d","NOEC","Growth rate (Chl a)",3,"Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject - bloom forming/nuisance and/or toxin producing blue-green algal species","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-14",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Anabaena oscillarioides 2","N.R.","8-36d","NOEC","Growth, yield (Chl a)",3,"Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject - bloom forming/nuisance and/or toxin producing blue-green algal species","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-15",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Nostoc muscorum 1","N.R.","7-11d","NOEC","Growth rate (Chl a)",">30 ","Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject - bloom forming/nuisance and/or toxin producing blue-green algal species","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-16",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Nostoc muscorum 1","N.R.","7-11d","NOEC","Growth, yield (Chl a)",">30 ","Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject - bloom forming/nuisance and/or toxin producing blue-green algal species","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-17",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Nostoc muscorum 2","N.R.","12-22d","NOEC","Growth rate (Chl a)",10,"Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject - bloom forming/nuisance and/or toxin producing blue-green algal species","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-18",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Nostoc muscorum 2","N.R.","12-22d","NOEC","Growth, yield (Chl a)",3,"Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject - bloom forming/nuisance and/or toxin producing blue-green algal species","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-19",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Nostoc punctiforme 1","N.R.","7-11d","NOEC","Growth rate (Chl a)",10,"Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject. This strain was cultured in medium with boron as a nutrient for many years prior.","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-20",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Nostoc punctiforme 1","N.R.","7-11d","NOEC","Growth, yield (Chl a)",10,"Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject. This strain was cultured in medium with boron as a nutrient for many years prior.","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-21",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Nostoc punctiforme 2","N.R.","6-26d","NOEC","Growth rate (Chl a)","<1 ","Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject.  Would need to use LOEC/2.5 conversion. Instead assess if yeild value can be used for thi species. Cannot re-calcualte EC10 as conc-response curve is flat. See notes in column z.","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-22",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Nostoc punctiforme 2","N.R.","6-26d","NOEC","Growth, yield (Chl a)",10,"Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Growth endpoint preferable, however, based on assessment of growth curves, yield acceptable for this species. Yield NOEC for any timepoint (on graph) is 10 mg/L, therefore reliable toxicity value.","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-23",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Microcystis aeruginosa","N.R.","7-13d","NOEC","Growth rate (Chl a)",">30 ","Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject - bloom forming/nuisance and/or toxin producing blue-green algal species","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-24",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Microcystis aeruginosa","N.R.","7-13d","NOEC","Growth, yield (Chl a)",">30 ","Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject - bloom forming/nuisance and/or toxin producing blue-green algal species","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-25",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Anabaena flos-aquae","N.R.","4-9d","NOEC","Growth rate (Chl a)",3,"Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject - bloom forming/nuisance and/or toxin producing blue-green algal species","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-26",57,0.57731958762886593,"Note: no acceptability criteria reported, also report states that B was measured beginning and end, but these values are NOT reported.","Anabaena flos-aquae","N.R.","4-9d","NOEC","Growth, yield (Chl a)",10,"Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.A.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Reject - bloom forming/nuisance and/or toxin producing blue-green algal species","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-27",56,0.57731958762886593,"The highest possible grade for this datum is acceptable.","Navicula sp 1.","N.R.","4-12 d","LOEC","Growth rate (Chl a)",1,"Microalga","Bacillariophyta","Bacillariophyceae ","Freshwater","20°C","Chronic","N.R.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Accept, however, only use if NOEC or EC10 not available, or if insufficient NOECs and EC10s for GV derivation as per Warne et al, 2018.","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-28",56,0.57731958762886593,"The highest possible grade for this datum is acceptable.","Synedra sp.","N.R.","4-18 d","LOEC","Growth rate (Chl a)",1,"Microalga","Bacillariophyta","Bacillariophyceae ","Freshwater","20°C","Chronic","N.R.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Accept, however, only use if NOEC or EC10 not available, or if insufficient NOECs and EC10s for GV derivation as per Warne et al, 2018.","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/plant","26-29",56,0.57731958762886593,"The highest possible grade for this datum is acceptable.","Nostoc punctiforme 2","N.R.","6-26d","LOEC","Growth rate (Chl a)",1,"Blue-green algae (Eubacteria)","Cyanobacteria","Cyanophyceae","Freshwater","20°C","Chronic","N.R.","N.A.","V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.","26","NR","BA","Accept, however, only use if NOEC or EC10 not available, or if insufficient NOECs and EC10s for GV derivation as per Warne et al, 2018.","Nominal. Report staes B was measured, but no reported concentrations"]
["Freshwater/metal/non- plant","27-1",79,0.79,null,"Ceriodaphnia dubia","neonates","7 d","NOEC","Reproduction ( number of young)",5.6,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","25°C","Chronic","7.5-8.5","N.A.","Hickey C.W., Macaskill, J.B. (1988). Huntley West No 1 Mine effluent ecotoxicity studies. Consultancy Report T7052, Water Quality Centre, DSIR Hamilton, NZ. ","27",44,"BA","Accept","Nominal, but two test concentrations were confirmed to be within 95% of measured.","Values are nominal. Stock concentrations of 32 and 180 mg/L were analysed: 34 and 186 mg/L. So there is confidence that actual concentrations are within 5% of nominal. Also dilution water measured to be 0 mg/L B."]
["Freshwater/metal/non- plant","27-2",79,0.79,"","Ceriodaphnia dubia","neonates","7 d","MATC","Reproduction ( number of young)",10,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","25°C","Chronic","7.5-8.5","N.A.","Hickey C.W., Macaskill, J.B. (1988). Huntley West No 1 Mine effluent ecotoxicity studies. Consultancy Report T7052, Water Quality Centre, DSIR Hamilton, NZ. ","27",44,"BA","Accept","Nominal, but two test concentrations were confirmed to be within 95% of measured."]
["Freshwater/metal/non- plant","27-3",79,0.79,"","Daphnia magna","neonates","14 d","NOEC","Reproduction ( number of young)",18,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","22°C","Chronic","7.5-8.5","N.A.","Hickey C.W., Macaskill, J.B. (1988). Huntley West No 1 Mine effluent ecotoxicity studies. Consultancy Report T7052, Water Quality Centre, DSIR Hamilton, NZ. ","27",44,"BA","Accept","Nominal, but two test concentrations were confirmed to be within 95% of measured."]
["Freshwater/metal/non- plant","27-4",79,0.79,"","Daphnia magna","neonates","14 d","MATC","Reproduction ( number of young)",24,"Micro-invertebrate","Arthropoda","Branchiopoda","Freshwater","22°C","Chronic","7.5-8.5","N.A.","Hickey C.W., Macaskill, J.B. (1988). Huntley West No 1 Mine effluent ecotoxicity studies. Consultancy Report T7052, Water Quality Centre, DSIR Hamilton, NZ. ","27",44,"BA","Accept","Nominal, but two test concentrations were confirmed to be within 95% of measured."]
["Freshwater/metal/plant","27-5",67,0.69072164948453607,"","Pseudokirchneriella subcapitata","N.R.","96 h","NOEC","Growth",2.8,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","24°C","Chronic","7.5-8.5","N.A.","Hickey C.W., Macaskill, J.B. (1988). Huntley West No 1 Mine effluent ecotoxicity studies. Consultancy Report T7052, Water Quality Centre, DSIR Hamilton, NZ. ","27",9.3000000000000007,"BA","Accept","Nominal, but two test concentrations were confirmed to be within 95% of measured."]
["Freshwater/metal/plant","27-6",67,0.69072164948453607,"","Pseudokirchneriella subcapitata","N.R.","96 h","MATC","Growth",5.7,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","24°C","Chronic","7.5-8.5","N.A.","Hickey C.W., Macaskill, J.B. (1988). Huntley West No 1 Mine effluent ecotoxicity studies. Consultancy Report T7052, Water Quality Centre, DSIR Hamilton, NZ. ","27",9.3000000000000007,"BA","Accept","Nominal, but two test concentrations were confirmed to be within 95% of measured."]
["Freshwater/metal/plant","27-7",67,0.69072164948453607,"","Pseudokirchneriella subcapitata","N.R.","8 d","NOEC","Growth",11.4,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","24°C","Chronic","7.5-8.5","N.A.","Hickey C.W., Macaskill, J.B. (1988). Huntley West No 1 Mine effluent ecotoxicity studies. Consultancy Report T7052, Water Quality Centre, DSIR Hamilton, NZ. ","27",9.3000000000000007,"BA","Accept","Nominal, but two test concentrations were confirmed to be within 95% of measured."]
["Freshwater/metal/plant","27-8",67,0.69072164948453607,"","Pseudokirchneriella subcapitata","N.R.","8 d","MATC","Growth",16.100000000000001,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","24°C","Chronic","7.5-8.5","N.A.","Hickey C.W., Macaskill, J.B. (1988). Huntley West No 1 Mine effluent ecotoxicity studies. Consultancy Report T7052, Water Quality Centre, DSIR Hamilton, NZ. ","27",9.3000000000000007,"BA","Accept","Nominal, but two test concentrations were confirmed to be within 95% of measured."]
["Freshwater/metal/plant","28-1",54,0.55670103092783507,"The highest possible grade for this datum is acceptable.","Eichhornia crassipes","Seed","21 d","NOEC","Germination",10,"Macrophyte","Angiosperm","Monocot","Freshwater","13h 35°C, 11h 10°C","Chronic","6.2-6.4","N.A.","Albano Perez E, Ruiz Tellez T and Sanchez Guzman JM (2011). Influence of physico-chemical parameters of the aquatic medium on germination of Eichhornia crassipes seeds. Plant Biol (Stuttg), 13(4), 643-648. . ","28",null,"BA","Reject","Nominal","Low quality paper. No B measurements in test waters, no proper control (but lowest B conc of 1 mg/L can be assumed to be control based on known essentiality of B). Also this species is a weed species (water hyacinth). "]
["Freshwater/metal/plant","29-1",75,0.77319587628865982,"The highest possible grade for this datum is acceptable.","Lemna minor","3-frond clones","7 d","NOEC","Growth (rate)",8,"Macrophyte","Angiosperm","Monocot","Freshwater","24°C","Chronic","N.R.","N.A.","Gur N, Turker OC and Bocuk H (2016). Toxicity assessment of boron (B) by Lemna minor L. and Lemna gibba L. and their possible use as model plants for ecological risk assessment of aquatic ecosystems with boron pollution. Chemosphere, 157, 1-9. ","29",null,"BA","Accept","Nominal","Nominal values, no B measurements. Control had 2 mg/L B in it as essential nutrient."]
["Freshwater/metal/plant","29-2",75,0.77319587628865982,"The highest possible grade for this datum is acceptable.","Lemna minor","3-frond clones","7 d","NOEC","biomass (dry weight)",16,"Macrophyte","Angiosperm","Monocot","Freshwater","24°C","Chronic","N.R.","N.A.","Gur N, Turker OC and Bocuk H (2016). Toxicity assessment of boron (B) by Lemna minor L. and Lemna gibba L. and their possible use as model plants for ecological risk assessment of aquatic ecosystems with boron pollution. Chemosphere, 157, 1-9. ","29",null,"BA","Accept","Nominal"]
["Freshwater/metal/plant","29-3",75,0.77319587628865982,"The highest possible grade for this datum is acceptable.","Lemna gibba","3-frond clones","7 d","NOEC","Growth (rate)",8,"Macrophyte","Angiosperm","Monocot","Freshwater","24°C","Chronic","N.R.","N.A.","Gur N, Turker OC and Bocuk H (2016). Toxicity assessment of boron (B) by Lemna minor L. and Lemna gibba L. and their possible use as model plants for ecological risk assessment of aquatic ecosystems with boron pollution. Chemosphere, 157, 1-9. ","29",null,"BA","Accept","Nominal"]
["Freshwater/metal/plant","29-4",75,0.77319587628865982,"The highest possible grade for this datum is acceptable.","Lemna gibba","3-frond clones","7 d","NOEC","biomass (dry weight)",32,"Macrophyte","Angiosperm","Monocot","Freshwater","24°C","Chronic","N.R.","N.A.","Gur N, Turker OC and Bocuk H (2016). Toxicity assessment of boron (B) by Lemna minor L. and Lemna gibba L. and their possible use as model plants for ecological risk assessment of aquatic ecosystems with boron pollution. Chemosphere, 157, 1-9. ","29",null,"BA","Accept","Nominal"]
["Freshwater/metal/plant","29-5",75,0.77319587628865982,"The highest possible grade for this datum is acceptable.","Lemna minor","3-frond clones","7 d","NOEC","Growth (yield)",8,"Macrophyte","Angiosperm","Monocot","Freshwater","24°C","Chronic","N.R.","N.A.","Gur N, Turker OC and Bocuk H (2016). Toxicity assessment of boron (B) by Lemna minor L. and Lemna gibba L. and their possible use as model plants for ecological risk assessment of aquatic ecosystems with boron pollution. Chemosphere, 157, 1-9. ","29",null,"BA","Accept","Nominal"]
["Freshwater/metal/plant","29-6",75,0.77319587628865982,"The highest possible grade for this datum is acceptable.","Lemna gibba","3-frond clones","7 d","NOEC","Growth (yield)",8,"Macrophyte","Angiosperm","Monocot","Freshwater","24°C","Chronic","N.R.","N.A.","Gur N, Turker OC and Bocuk H (2016). Toxicity assessment of boron (B) by Lemna minor L. and Lemna gibba L. and their possible use as model plants for ecological risk assessment of aquatic ecosystems with boron pollution. Chemosphere, 157, 1-9. ","29",null,"BA","Accept","Nominal"]
["Freshwater/metal/plant","30-1",58,0.59793814432989689,"The highest possible grade for this datum is acceptable. Note - acceptability criteria not stated or inferred. ","Lemna gibba","N.R.","10 d","NOEC","biomass (dry weight)",0.75,"Macrophyte","Angiosperm","Monocot","Freshwater","25-29°C","Chronic","N.R.","N.A.","Villavicencio MS, Silva, C.A. Arce, G.M. (2007). Boron toxicity in Mena gibba. Hidrobologica, 17, 1-6. ","30",null,"BA","Reject","Nominal","Low quality paper. This test was done according to Penningsfield and Kurzman 1975 methods (a spanish book - I could not read). No acceptability criteria in the article. However, there was only a 1.4-fold increase in the Controls from Day 0 to Day 10. OECD guidelines suggest a minimum of 7-fold increase for Lemna gibba over 7 days. USEPA reccomends a doubling time of 2.5 days. The Lemna in this study did not double at all. B was not measured in test waters, but were measured in plants for accumulation studies. Tests used 1/40th strength Hoaglands medium which has around 12.5 µg B/L in it. Tests were conducted in polypropylene, so no borosilicate glass leaching. "]
["Freshwater/metal/plant","31-1",80,0.82474226804123707,"","Lemna disperma","N.R.","7 d","EC10","Growth",1.4,"Macrophyte","Angiosperm","Monocot","Freshwater","25°C","Chronic","7.0-7.2","N.A.","Acqua Della Vita (2014). Condomine River - DTA of boron and treated CSG water, Final report. Acqua Della Vita, Queensland, Australia. 34 pp.","31",null,"BA","Accept","Measured","New data: Report done by Heather Chapman for Origin Energy. Tests done in Condomine River water, upstream of intended CSG impacts. Ceriodaphnia 7-d data not included due to failed acceptability criteria (refernce toxicant IC50 below limits). Condomine River water B concentration always low. Note for Fish and Selenastrum tests, there were hormesis effects at low concentrations of B, in agreement with previous essentiality studies. Condomine R water contained low concentrations of some additional metals, but these are accounted for with extra RIver water controls and use of this water as a diluent."]
["Freshwater/metal/plant","31-2",80,0.82474226804123707,"","Lemna disperma","N.R.","7 d","NOEC","Growth",3.6,"Macrophyte","Angiosperm","Monocot","Freshwater","25°C","Chronic","7.0-7.2","N.A.","Acqua Della Vita (2014). Condomine River - DTA of boron and treated CSG water, Final report. Acqua Della Vita, Queensland, Australia. 34 pp.","31",null,"BA","Accept","Measured"]
["Freshwater/metal/plant","31-3",80,0.82474226804123707,"","Pseudokirchneriella subcapitata","N.R.","72 h","IC10","Growth",13,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","25°C","Chronic","7.7-8.1","N.A.","Acqua Della Vita (2014). Condomine River - DTA of boron and treated CSG water, Final report. Acqua Della Vita, Queensland, Australia. 34 pp.","31",null,"BA","Accept","Measured"]
["Freshwater/metal/plant","31-4",80,0.82474226804123707,"","Pseudokirchneriella subcapitata","N.R.","72 h","NOEC","Growth",9.5,"Microalga","Chlorophyta","Chlorophyceae","Freshwater","25°C","Chronic","7.7-8.1","N.A.","Acqua Della Vita (2014). Condomine River - DTA of boron and treated CSG water, Final report. Acqua Della Vita, Queensland, Australia. 34 pp.","31",null,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","31-5",79,0.79,"","Melanotaenia spledida","embryo","12 d","EC10","Mortality",102,"Fish","Chordata","Actinopterygii","Freshwater","25 ± 1°C","Chronic","6.9-7.5","N.A.","Acqua Della Vita (2014). Condomine River - DTA of boron and treated CSG water, Final report. Acqua Della Vita, Queensland, Australia. 34 pp.","31",null,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","31-5",83,0.83,"","Melanotaenia spledida","embryo","12 d","NOEC","Mortality",110,"Fish","Chordata","Actinopterygii","Freshwater","25 ± 1°C","Chronic","6.9-7.5","N.A.","Acqua Della Vita (2014). Condomine River - DTA of boron and treated CSG water, Final report. Acqua Della Vita, Queensland, Australia. 34 pp.","31",null,"BA","Accept","Measured"]
["Freshwater/metal/non- plant","32-1",85,0.85,"","Cirrhinus mrigala","Advanced fry","50 d","NOEC","Mortality",8,"Fish","Chordata","Actinopterygii","Freshwater","26 ± 3","Chronic","7.4 ± 0.1","N.A.","Adhikiri, S. and Mohanty, M. (2012). Effect of waterborne boron and molybdenum on survival, growth and feed intake of Indian major carp, Cirrhinus mrigala (Hamilton). Chemistry and Ecology 28:113-121.","32",null,"Na2B4O7 · 10H2O","Accept","Measured","Note 8 mg/L was highest test concentration, survival was reduced by 12%, but this was not significantly different. "]
["Freshwater/metal/non- plant","32-2",85,0.85,"","Cirrhinus mrigala","Advanced fry","50 d","NOEC","Specific growth rate (%.d)",4,"Fish","Chordata","Actinopterygii","Freshwater","26 ± 3","Chronic","7.4 ± 0.1","N.A.","Adhikiri, S. and Mohanty, M. (2012). Effect of waterborne boron and molybdenum on survival, growth and feed intake of Indian major carp, Cirrhinus mrigala (Hamilton). Chemistry and Ecology 28:113-121.","32",null,"Na2B4O7 · 10H2O","Accept","Measured"]
["Freshwater/metal/non- plant","33-1",64,0.64,"Note - acceptability criteria not stated or inferred. ","Ambystoma jeffersonian","Embryo (blastula)",25,"LOEC","Nomral larval development",49.5,"Amphibian","Chordata","Amphibia","Freshwater","5-10°C","Chronic",6.5,"N.A.","Laposata, M. M.; Dunson, W.A. 1998: Effects of boron and nitrate on hatching success of amphibian eggs. Department of Biology, Pennsylvania State University, Pennsylvania, USDA. Archives of Environmental Contamination and Toxicology, 35, 615-619.","33",null,"Na2B4O7 · 10H2O","Reject","Nominal","This study assessed hatching success and larval abnormalities in four species at low test temperature (5-10°C). No acceptability criteria stated, but 82-97% hatching success recorded for controls, and 99-100% normal larval development. Only two B concentrations were tested against a control, 5 replicates per treatment. B was measured as 49.5 and 100.24 mg/L (unlcear if total or dissolved B). <5% change between start and end of test for B concs.\n\nInappropriate conc range (2 concentrations) tested, resulting in unreliable LOEC values. The four LOECs of 49.5 mg/L corresponded to a 44% effect (B. americanus hatching success), 48% effect (R sylvatica larval development), 7% effect (A jeffersonianum LD) and 18% effect (A. maculatum LD). Since Warne et al (2018) states that converted LOECs and EC50s should only be used when insufficent EC10s/NOECs available to derive the GV, all four LOECs were rejected.\n"]
["Freshwater/metal/non- plant","33-2",64,0.64,"Note - acceptability criteria not stated or inferred. ","Rana sylvatica","Embryo (blastula)",23,"LOEC","Normal larval development",49.5,"Amphibian","Chordata","Amphibia","Freshwater","5-10°C","Chronic",6.5,"N.A.","Laposata, M. M.; Dunson, W.A. 1998: Effects of boron and nitrate on hatching success of amphibian eggs. Department of Biology, Pennsylvania State University, Pennsylvania, USDA. Archives of Environmental Contamination and Toxicology, 35, 615-619.","33",null,"Na2B4O7 · 10H2O","Reject","Nominal"]
["Freshwater/metal/non- plant","33-3",64,0.64,"Note - acceptability criteria not stated or inferred. ","Ambystonium maculatum","Embryo (blastula)",44,"LOEC","Normal larval development",49.5,"Amphibian","Chordata","Amphibia","Freshwater","5-10°C","Chronic",6.5,"N.A.","Laposata, M. M.; Dunson, W.A. 1998: Effects of boron and nitrate on hatching success of amphibian eggs. Department of Biology, Pennsylvania State University, Pennsylvania, USDA. Archives of Environmental Contamination and Toxicology, 35, 615-619.","33",null,"Na2B4O7 · 10H2O","Reject","Nominal"]
["Freshwater/metal/non- plant","33-4",64,0.64,"Note - acceptability criteria not stated or inferred. ","Bufo americanus","Embryo (blastula)",23,"LOEC","Hatching success",49.5,"Amphibian","Chordata","Amphibia","Freshwater","10°C","Chronic",6.5,"N.A.","Laposata, M. M.; Dunson, W.A. 1998: Effects of boron and nitrate on hatching success of amphibian eggs. Department of Biology, Pennsylvania State University, Pennsylvania, USDA. Archives of Environmental Contamination and Toxicology, 35, 615-619.","33",null,"Na2B4O7 · 10H2O","Reject","Nominal"]

{"sheet":"FW metal nonplant_WS","index":5,"n_rows":203,"n_cols":26,"rows_kept":203,"cut":false,"hidden":false,"merged":[]}
[]
["Question No.",1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16.100000000000001,16.2,16.3,16.399999999999999,16.600000000000001,17,18,"Total Score","Quality Score"]
["Short Questions","Duration of the exposure ","Biological endpoint","Biological effect stated or not","Biological effect quantified or not","Appropriate controls","Control & contaminant concentration","Test acceptability criteria","Characteristics of the test organism"," Type of test media","Type of exposure","Contaminant concentrations","Parallel reference toxicant toxicity tests","Concentration–response relationship","Determine the toxicity","NOEC/LOEC or LC/EC/BEC","pH","Hardness","Alkalinity","Organic carbon concentration","Dissolved Oxygen","Temperature","Grade or purity"]
["Marks Awarded ","10 or 0","10,5 or 0","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","4 or 0","4 or 2 or 0","4 or 2","4 or 2","4 or 2","4 or 2","3 or 1 or 0","3 or 1 or 0","3 or 1 or 0","3 or 1 or 0","3 or 1 or 0","3 or 0","3 or 0 "]
["Max. Possible Score",10,10,5,5,5,5,5,5,5,4,4,4,4,4,4,3,3,3,3,3,3,3,{"$f":"SUM(B5:W5)","v":100}]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","3-1"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,78,0.78,""]
["Answers","8d post hatch + average 3d to hatch = 11 d","Mortality","LC",10,"Non toxicant controls","2-4 replicates","95% survival in controls","Embryo - immediately after fertilisation to 8d post hatch","Reconstituted water hardness 200 mg/L CaCO3, described in Birge and Cassidy 1983","Flow through","Measured at 1-2 d intervals","","Yes","Modified method in Dyer et al.","",7.5,"204 mg/L CaCO3","Measured but not stated","","8.4 mg/L","20°C","Controls had no Boron detected"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","3-2"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,78,0.78,""]
["Answers","32 d","Mortality","LC",10,"Non toxicant controls","2-6 replicates","","Embryo - from 2-4h post fertilisation to 8d post hatch","Reconstituted hard water","Flow through","Measured throughout, 2-3 days","","Yes","Modified method in Dyer et al.","",7.4,"197 mg CaCO3/L","","","9.8 mg/L",13.2,"Boron not detected in control water"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","3-3"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,0,3,3,0,3,3,3,3,81,0.81,""]
["Answers","32 d","Mortality","LC",10,"Non toxicant controls","2-6 replicates","93% survival in controls","Embryo - from 2-4h post fertilisation to 8d post hatch","Reconstituted hard water","Flow through","Measured throughout, 2-3 days","","Yes","Modified method in Dyer et al.","","7.7 ± 0.2","188 mg CaCO3/L","","<0.5 mg/L","10 ± 0.3 mg/L","13.7 ± 0.3°C","Reagent grade Boric acid"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-1"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,73,0.73,""]
["Answers","28 d","Mortality and/or teratogenic","LC",10,"Non toxicant controls","Not stated although likely.",">90% survival","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","Yes","Modified method in Dyer et al.","","7.7 ± 0.1","54 ± 3.5 mg CaCO3/L","","","9.2 ± 0.1 mg/L","13.7 ± 0.1°C","ACS grade Boric acid"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-2"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,73,0.73,""]
["Answers","28 d","Mortality and/or teratogenic","LC",10,"Non toxicant controls","Not stated although likely.",">90% survival","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","Yes","Modified method in Dyer et al.","","7.9 ± 0.1","49 ± 1.4 mg CaCO3/L","","","10.1 ± 0.1 mg/L","14 ± 0.1°C","ACS grade Borax"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-3"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,73,0.73,""]
["Answers","28 d","Mortality and/or teratogenic","LC",10,"Non toxicant controls","Not stated although likely.",">90% survival","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","Yes","Modified method in Dyer et al.","","7.8 ± 0.1","191 ± 4.0 mg CaCO3/L","","","10.3 ± 0.2 mg/L","13 ± 0.1°C","ACS grade Borax"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-4"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,73,0.73,""]
["Answers","9 d","Mortality and/or teratogenic","LC",10,"Non toxicant controls","Not stated although likely.",">90% survival","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","Yes","Modified method in Dyer et al.","","7.5 ± 0.1","51.8 ± 2.0 mg CaCO3/L","","","7.3 ± 0.1 mg/L","25 ± 0.4°C","ACS grade Boric acid"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-5"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,73,0.73,""]
["Answers","9 d","Mortality and/or teratogenic","LC",20,"Non toxicant controls","Not stated although likely.",">90% survival","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","Yes","Modified method in Dyer et al.","","7.6 ± 0.0","212 ± 11.0 mg CaCO3/L","","","7.6 ± 0.1 mg/L","24.7 ± 0.6°C","ACS grade Boric acid"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-6"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,73,0.73,""]
["Answers","9 d","Mortality and/or teratogenic","LC",10,"Non toxicant controls","Not stated although likely.",">90% survival","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","Yes","Modified method in Dyer et al.","","8.5 ± 0.0","46.7 ± 2.3 mg CaCO3/L","","","6.4 ± 0.1 mg/L","29.4 ± 0.3°C","ACS grade Borax"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-7"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,73,0.73,""]
["Answers","9 d","Mortality and/or teratogenic","LC",10,"Non toxicant controls","Not stated although likely.",">90% survival","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","Yes","Modified method in Dyer et al.","","8.2 ± 0.1","194.7 ± 3.2 mg CaCO3/L","","","6.5 ± 0.1 mg/L","29.4 ± 0.3°C","ACS grade Borax"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-8"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,73,0.73,""]
["Answers","7.5 d","Mortality and/or teratogenic","LC",10,"Non toxicant controls","Not stated although likely.",">80% survival","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","Yes","Modified method in Dyer et al.","","7.7 ± 0.0","52.5 ± 1.4 mg CaCO3/L","","","7.7 ± 0.2 mg/L","25.0 ± 0.3°C","ACS grade Boric acid"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-9"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,73,0.73,""]
["Answers","7.5 d","Mortality and/or teratogenic","LC",10,"Non toxicant controls","Not stated although likely.",">80% survival","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","Yes","Modified method in Dyer et al.","","7.7 ± 0.0","212.3 ± 5.4 mg CaCO3/L","","","7.8 ± 0.2 mg/L","25.0 ± 0.3°C","ACS grade Boric acid"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-10"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,73,0.73,""]
["Answers","7.5 d","Mortality and/or teratogenic","LC",10,"Non toxicant controls","Not stated although likely.",">80% survival","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","Yes","Modified method in Dyer et al.","","8.3 ± 0.1","46.2 ± 4.9 mg CaCO3/L","","","7.7 ± 0.2 mg/L","25.3 ± 0.2°C","ACS grade Borax"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-11"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,73,0.73,""]
["Answers","7.5 d","Mortality and/or teratogenic","LC",10,"Non toxicant controls","Not stated although likely.",">80% survival","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","Yes","Modified method in Dyer et al.","","8.4 ± 0.1","203 ± 2.3 mg CaCO3/L","","","7.8 ± 0.2 mg/L","25.3 ± 0.2°C","ACS grade Borax"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-12"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,73,0.73,""]
["Answers","7.5 d","Mortality and/or teratogenic","LC",10,"Non toxicant controls","Not stated although likely.",">80% survival","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","Yes","Modified method in Dyer et al.","","7.6 ± 0.1","57.4 ± 4.2 mg CaCO3/L","","","6.8 ± 0.1 mg/L","23.7 ± 0.6°C","ACS grade Boric acid"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-13"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,0,3,3,0,0,3,3,3,73,0.73,""]
["Answers","7.5 d","Mortality and/or teratogenic","LC",10,"Non toxicant controls","Not stated although likely.",">80% survival","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","Yes","Modified method in Dyer et al.","","7.6 ± 0.1","207.5 ± 10 mg CaCO3/L","","","6.8 ± 0.1 mg/L","23.7 ± 0.6°C","ACS grade Boric acid"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","11-1"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,0,3,3,3,3,85,0.85,""]
["Answers","34 d","Mortality","NOEC","Tested at 0.05 and 0.01.","Diluent controls","quadruplicate","OECD guidelines. No mortality in controls reported.","Embryonic stage at the start of the test was confrimed to be blastula","DSWL-E water. Recipe provided.","semi-static, renewals three times per week.","Measured beginning and end","Not mentioned","","","0.05 and 0.01","7.2-8.0","212 mg/L CaCO3/L","","1.6 mg/L","lowest was 7.1 mg/L","24.1-25.7°C","99.9% purity and controls were tested for B content."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","11-2"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,0,3,3,3,3,85,0.85,""]
["Answers","34 d","length","NOEC","Tested at 0.05 and 0.01.","Diluent controls","quadruplicate","OECD guidelines. No mortality in controls reported.","Embryonic stage at the start of the test was confrimed to be blastula","DSWL-E water. Recipe provided.","semi-static, renewals three times per week.","Measured beginning and end","Not mentioned","","","0.05 and 0.01","7.2-8.0","212 mg/L CaCO3/L","","1.6 mg/L","lowest was 7.1 mg/L","24.1-25.7°C","99.9% purity and controls were tested for B content."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","11-3"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,0,3,3,3,3,85,0.85,""]
["Answers","34 d","dry weight ","NOEC","Tested at 0.05 and 0.01.","Diluent controls","quadruplicate","OECD guidelines. No mortality in controls reported.","Embryonic stage at the start of the test was confrimed to be blastula","DSWL-E water. Recipe provided.","semi-static, renewals three times per week.","Measured beginning and end","Not mentioned","","","0.05 and 0.01","7.2-8.0","212 mg/L CaCO3/L","","1.6 mg/L","lowest was 7.1 mg/L","24.1-25.7°C","99.9% purity and controls were tested for B content."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","12-1"]
["Scores",10,5,5,0,5,5,2,0,5,0,4,0,0,4,0,3,3,0,0,0,3,3,57,0.56999999999999995,""]
["Answers","21 d","reproduction","NOEC","Originbal report not available, so significance level not found for NOEC effect.","DSWL-E controls","Carried out according to OECD, therefore 10 replicates assumed","OECD","Maybe in report, but not available at present.","DSWL-E media","","Measured values were within 20% of nominal, so nominal value reported","","Probably was, however original report not available","Assume so since OECD","Probably but original report not available.","7.2-8.0","212 mg/L","","Likely was measured and stated, but original report not available","Likely was measured and stated, but original report not available","19.3-20.9°C",">99.9% purity, controls measured"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","13-1"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,1,3,3,0,3,3,3,88,0.88,""]
["Answers","21 d","number of young produced by each adult","NOEC from dataset based on stats","p = 0.05","Control included","quadruplicate, with 5 animals in each rep. 20 animals altogether per treatment","100% survival in Controls","<24h old neonates","Lake Huron water, filtered, UV-irradiates, hardness adjusted, autoclaved.","Static renewal","Measured throughout.","","","",0.05,"Measured but not reported for chronic test. Stated to be 8.1 ± 0.1 in Control water. Only pH values for acute test reported.","148 ± 7 mg/L CaCO3","58 ± 5 mg/L CaCO3/L","","7.3-8.0 mg/L","19.5-20.5°C","Controls measured - mean analyzed B conc was 0 mg/L."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","13-2"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,1,3,3,0,3,3,3,88,0.88,""]
["Answers","21 d","mean number of total broods","NOEC from dataset based on stats","p = 0.05","Control included","quadruplicate, with 5 animals in each rep. 20 animals altogether per treatment","100% survival in Controls","<24h old neonates","Lake Huron water, filtered, UV-irradiates, hardness adjusted, autoclaved.","Static renewal","Measured throughout.","","","",0.05,"Measured but not reported for chronic test. Stated to be 8.1 ± 0.1 in Control water. Only pH values for acute test reported.","148 ± 7 mg/L CaCO3","58 ± 5 mg/L CaCO3/L","","7.3-8.0 mg/L","19.5-20.5°C","Controls measured - mean analyzed B conc was 0 mg/L."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","13-3"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,1,3,3,0,3,3,3,88,0.88,""]
["Answers","21 d","mean number of total broods","NOEC from dataset based on stats","p = 0.05","Control included","quadruplicate, with 5 animals in each rep. 20 animals altogether per treatment","100% survival in Controls","<24h old neonates","Lake Huron water, filtered, UV-irradiates, hardness adjusted, autoclaved.","Static renewal","Measured throughout.","","","",0.05,"Measured but not reported for chronic test. Stated to be 8.1 ± 0.1 in Control water. Only pH values for acute test reported.","148 ± 7 mg/L CaCO3","58 ± 5 mg/L CaCO3/L","","7.3-8.0 mg/L","19.5-20.5°C","Controls measured - mean analyzed B conc was 0 mg/L."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","13-4"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,1,3,3,0,3,3,3,83,0.83,""]
["Answers","21 d","Survival","NOEC from dataset based on stats","p = 0.05","Control included","quadruplicate, with 5 animals in each rep. 20 animals altogether per treatment","100% survival in Controls","<24h old neonates","Lake Huron water, filtered, UV-irradiates, hardness adjusted, autoclaved.","Static renewal","Measured throughout.","","","",0.05,"Measured but not reported for chronic test. Stated to be 8.1 ± 0.1 in Control water. Only pH values for acute test reported.","148 ± 7 mg/L CaCO3","58 ± 5 mg/L CaCO3/L","","7.3-8.0 mg/L","19.5-20.5°C","Controls measured - mean analyzed B conc was 0 mg/L."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","13-5"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,1,3,3,0,3,3,3,83,0.83,""]
["Answers","21 d","Length","NOEC from dataset based on stats","p = 0.05","Control included","quadruplicate, with 5 animals in each rep. 20 animals altogether per treatment","100% survival in Controls","<24h old neonates","Lake Huron water, filtered, UV-irradiates, hardness adjusted, autoclaved.","Static renewal","Measured throughout.","","","",0.05,"Measured but not reported for chronic test. Stated to be 8.1 ± 0.1 in Control water. Only pH values for acute test reported.","148 ± 7 mg/L CaCO3","58 ± 5 mg/L CaCO3/L","","7.3-8.0 mg/L","19.5-20.5°C","Controls measured - mean analyzed B conc was 0 mg/L."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","14-1"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,1,0,0,3,3,3,85,0.85,""]
["Answers","14 d","Reproduction (number of young per animal)","NOEC","p 0.05","Diluent controls","quadruplicate with 5 per rep, 20 animals per treatment. And test repeated.","Aimed to improve ASTM and OECD methods. 0% mortality in controls.","<24h old neonates","Lake Huron water, obtained from Midland Water Treatment Plant prior to chlorination, adjusted to 170 mg/L hardness and then autoclaved.","static renewal (3 times per week).","Measurd throughout.","","","",0.05,"7.3-8.2","170 mg CaCO3/L","","",">90% saturation","23-25.2°C","Controls were measured. Reagent grade H3BO3 used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","14-2"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,1,0,0,3,3,3,85,0.85,""]
["Answers","14 d","Reproduction (mean brood size per adult)","NOEC","p 0.05","Diluent controls","quadruplicate with 5 per rep, 20 animals per treatment. And test repeated.","Aimed to improve ASTM and OECD methods. 0% mortality in controls.","<24h old neonates","Lake Huron water, obtained from Midland Water Treatment Plant prior to chlorination, adjusted to 170 mg/L hardness and then autoclaved.","static renewal (3 times per week).","Measurd throughout.","","","",0.05,"7.3-8.2","170 mg CaCO3/L","","",">90% saturation","23-25.2°C","Controls were measured. Reagent grade H3BO3 used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","14-3"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,3,1,0,0,3,3,3,80,0.8,""]
["Answers","14 d","Mortality","NOEC","p 0.05","Diluent controls","quadruplicate with 5 per rep, 20 animals per treatment. And test repeated.","Aimed to improve ASTM and OECD methods. 0% mortality in controls.","<24h old neonates","Lake Huron water, obtained from Midland Water Treatment Plant prior to chlorination, adjusted to 170 mg/L hardness and then autoclaved.","static renewal (3 times per week).","Measurd throughout.","","","",0.05,"7.3-8.2","170 mg CaCO3/L","","",">90% saturation","23-25.2°C","Controls were measured. Reagent grade H3BO3 used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","14-4"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,3,1,0,0,3,3,3,80,0.8,""]
["Answers","14 d","Dry weight.","NOEC","p 0.05","Diluent controls","quadruplicate with 5 per rep, 20 animals per treatment. And test repeated.","Aimed to improve ASTM and OECD methods. 0% mortality in controls.","<24h old neonates","Lake Huron water, obtained from Midland Water Treatment Plant prior to chlorination, adjusted to 170 mg/L hardness and then autoclaved.","static renewal (3 times per week).","Measurd throughout.","","","",0.05,"7.3-8.2","170 mg CaCO3/L","","",">90% saturation","23-25.2°C","Controls were measured. Reagent grade H3BO3 used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","14-5"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,1,0,0,3,3,3,85,0.85,""]
["Answers","14 d","Number of young per adult","NOEC","p 0.05","Diluent controls","quadruplicate with 5 per rep, 20 animals per treatment. And test repeated.","Aimed to improve ASTM and OECD methods. 0% mortality in controls.","<24h old neonates","Lake Huron water, obtained from Midland Water Treatment Plant prior to chlorination, adjusted to 170 mg/L hardness and then autoclaved.","static renewal (3 times per week).","Measurd throughout.","","","",0.05,"7.3-8.2","170 mg CaCO3/L","","",">90% saturation","23-25.2°C","Controls were measured. Reagent grade H3BO3 used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","14-6"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,1,0,0,3,3,3,85,0.85,""]
["Answers","14 d","mean brood per adult","NOEC","p 0.05","Diluent controls","quadruplicate with 5 per rep, 20 animals per treatment. And test repeated.","Aimed to improve ASTM and OECD methods. 0% mortality in controls.","<24h old neonates","Lake Huron water, obtained from Midland Water Treatment Plant prior to chlorination, adjusted to 170 mg/L hardness and then autoclaved.","static renewal (3 times per week).","Measurd throughout.","","","",0.05,"7.3-8.2","170 mg CaCO3/L","","",">90% saturation","23-25.2°C","Controls were measured. Reagent grade H3BO3 used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","14-7"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,3,1,0,0,3,3,3,80,0.8,""]
["Answers","14 d","mortality","NOEC","p 0.05","Diluent controls","quadruplicate with 5 per rep, 20 animals per treatment. And test repeated.","Aimed to improve ASTM and OECD methods. 0% mortality in controls.","<24h old neonates","Lake Huron water, obtained from Midland Water Treatment Plant prior to chlorination, adjusted to 170 mg/L hardness and then autoclaved.","static renewal (3 times per week).","Measurd throughout.","","","",0.05,"7.3-8.2","170 mg CaCO3/L","","",">90% saturation","23-25.2°C","Controls were measured. Reagent grade H3BO3 used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","14-8"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,3,1,0,0,3,3,3,80,0.8,""]
["Answers","14 d","Dry weight","NOEC","p 0.05","Diluent controls","quadruplicate with 5 per rep, 20 animals per treatment. And test repeated.","Aimed to improve ASTM and OECD methods. 0% mortality in controls.","<24h old neonates","Lake Huron water, obtained from Midland Water Treatment Plant prior to chlorination, adjusted to 170 mg/L hardness and then autoclaved.","static renewal (3 times per week).","Measurd throughout.","","","",0.05,"7.3-8.2","170 mg CaCO3/L","","",">90% saturation","23-25.2°C","Controls were measured. Reagent grade H3BO3 used."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","15-1"]
["Scores",10,10,5,5,5,5,0,5,5,4,0,0,0,4,4,3,3,0,0,0,1,3,72,0.72,"The highest possible grade for this datum is acceptable."]
["Answers","14 d","total number of young per female","NOEC","p - 0.05","Diluent controls","10 replicates on one per vial.","","<24h old","HMSO 1983, British Standard, 1983 water.","Static renewal, (transferred every 48h).","","","Final toxicity values reported, but no response curves.","",0.05,"7.9 ± 0.2","250 ± 25 mg/L CaCO3","","","","20°C","AR Grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","15-2"]
["Scores",10,10,5,5,5,5,0,5,5,4,0,0,0,4,4,3,3,0,0,0,1,3,72,0.72,"The highest possible grade for this datum is acceptable."]
["Answers","14 d","total number of young per female","NOEC","p - 0.05","Diluent controls","10 replicates on one per vial.","","<24h old","HMSO 1983, British Standard, 1983 water.","Static renewal, (transferred every 48h).","","","Final toxicity values reported, but no response curves.","",0.05,"7.9 ± 0.2","250 ± 25 mg/L CaCO3","","","","20°C","AR Grade"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","16-1"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,0,0,3,3,3,87,0.87,""]
["Answers","21 d","Reproduction (number of young)","NOEC","p 0.05","Diluent controls","survival and growth measurements:  7 replicates on individual animals, reproduction assessment: 3 replicates of five animals in each. Control had 6x5 and 14x1 animals.","Control mortality 9%.","<24 h","Carbon-filtered well water (Maki and Bishop, 1979).","Static renewal (Mon, Wed, Fri).","Measured throughout and were within 95% of nominal. Values reported as mean-measure, control-corrected values.","","","",0.05,"7.1-8.7","166 mg CaCO3/l (range = 135-217).","","",">9 mg/L, >90% saturation.","19.2°C, range 18-21","Analytical grade boric acid"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","16-2"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,0,0,3,3,3,87,0.87,""]
["Answers","21 d","Length was emasured from apex of helmet to base of spine to nearest 0.1mm.","NOEC","p 0.05","Diluent controls","survival and growth measurements:  7 replicates on individual animals, reproduction assessment: 3 replicates of five animals in each. Control had 6x5 and 14x1 animals.","Control mortality 9%.","<24 h","Carbon-filtered well water (Maki and Bishop, 1979).","Static renewal (Mon, Wed, Fri).","Measured throughout and were within 95% of nominal. Values reported as mean-measure, control-corrected values.","","","",0.05,"7.1-8.7","166 mg CaCO3/l (range = 135-217).","","",">9 mg/L, >90% saturation.","19.2°C, range 18-21","Analytical grade boric acid"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","17-1"]
["Scores",10,5,5,5,5,0,0,5,5,0,2,0,4,4,4,0,0,0,0,0,1,3,58,0.57999999999999996,""]
["Answers","from fertilisation until 2 weeks post-hatch (6 weeks).","Survival","NOAEL determined via the LOAEL and reported concetration series.","p 0.05","","Suspect it was but it is not reported. There are error bars on curves, suggesting replication.","","fertilized eggs","ASTM ultrapure grade water","","Doesn’t state whether measured more than once or not.","","U shape response curve.","",0.05,"","","","","","12.5°C","B measured in controls"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","19-1"]
["Scores",10,10,5,5,5,0,0,5,5,4,0,0,4,4,4,1,1,0,0,1,1,3,68,0.68,"The highest possible grade for this datum is acceptable."]
["Answers","283h (11.8 d)","During bioassays, the criterion for death was cessation of all movement, including respiratory movement","LC",50,"Diluent controls","Unclear. 20 fish per tank, but how many tanks per treatment?","","0.19-0.7g alevins","Aerated well water","Static renewal (24h renewal)","Unclear - B measurements were done, but not in test solutions it seems. Only for tissue anlayses and effluent samples.","","","","95% CLs 104-123 mg/L","Measured but not stated","47 mg/L CaCO3","","","stated as measured but not reported","Measured, but not reported. Stated as 11 ± 0.5°C","Analytical grade Na2B2O4.8H2O"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","20-1"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,0,1,3,3,91,0.91,""]
["Answers","21 d","total shell length ( longest axis) under 50x magnification to the nearest 0.01 mm.","NOEC","p 0.05","Diluent controls","8 replicates","ASTM methods and stated that test acceptability criteria were met.","3 months old","USEPA moderately hard water","static renewal (dialy renewal).","Measured before and after renewal on 4 occasions. Mean conc was within 5% of nominal so nominal used in analyses.","","","","p 0.05","6.82-7.93","89-108 mg/L CaCO3","45-50 at start but dropped to 15-20 in some by end of test.","","Measured daily but not reported.","18.9-21.7°C","Measured in controls. Raneg from <0.19-0.03 mg/L."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","20-2"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,0,3,3,3,0,1,3,3,87,0.87,""]
["Answers","21 d","total shell length ( longest axis) under 50x magnification to the nearest 0.01 mm.","IC",25,"Diluent controls","8 replicates","ASTM methods and stated that test acceptability criteria were met.","3 months old","USEPA moderately hard water","static renewal (dialy renewal).","Measured before and after renewal on 4 occasions. Mean conc was within 5% of nominal so nominal used in analyses.","","","","no CLs","6.82-7.93","89-108 mg/L CaCO3","45-50 at start but dropped to 15-20 in some by end of test.","","Measured daily but not reported.","18.9-21.7°C","Measured in controls. Raneg from <0.19-0.03 mg/L."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","20-3"]
["Scores",10,5,5,5,5,5,5,5,5,4,4,0,4,4,4,3,3,3,0,1,3,3,86,0.86,""]
["Answers","21 d","Mortality","NOEC","p 0.05","Diluent controls","8 replicates","ASTM methods and stated that test acceptability criteria were met.","3 months old","USEPA moderately hard water","static renewal (dialy renewal).","Measured before and after renewal on 4 occasions. Mean conc was within 5% of nominal so nominal used in analyses.","","","",0.05,"6.82-7.93","89-108 mg/L CaCO3","45-50 at start but dropped to 15-20 in some by end of test.","","Measured daily but not reported.","18.9-21.7°C","Measured in controls. Raneg from <0.19-0.03 mg/L."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","20-4"]
["Scores",10,5,5,5,5,5,5,5,5,4,4,0,4,4,0,3,3,3,0,1,3,3,82,0.82,""]
["Answers","21 d","Mortality","IC",25,"Diluent controls","8 replicates","ASTM methods and stated that test acceptability criteria were met.","3 months old","USEPA moderately hard water","static renewal (dialy renewal).","Measured before and after renewal on 4 occasions. Mean conc was within 5% of nominal so nominal used in analyses.","","","","No CLs","6.82-7.93","89-108 mg/L CaCO3","45-50 at start but dropped to 15-20 in some by end of test.","","Measured daily but not reported.","18.9-21.7°C","Measured in controls. Raneg from <0.19-0.03 mg/L."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","22-1"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,1,1,0,0,3,1,3,76,0.76,""]
["Answers","42 d","Mortality","NOEC","p 0.05","Diluent cotnrols","4 reps","USEPA methods, some acceptability criteria reported (water quality).","7- to 14-d old","100b media (recipe provided)","static renewal, 3-4 day renewals","Measured throughout. Measured values were within 3.5% of nominal.","","","",0.05,"8.1. Measured throughout but not reported.","Measured throughout and in acute test, but not reported for chronic. Hardness in acute test (same media) was 106 mg CaCO3/L","","",">6.6 mg/L across all treatments.","Measured but not reported. Stated as 22°C.","Controls were measured. Values not given, but refers to report not publically available."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","22-2"]
["Scores",10,10,5,5,5,5,2,5,5,4,4,0,4,4,4,1,1,0,0,3,1,3,81,0.81,""]
["Answers","42 d","Reproduction (number of young per female)","NOEC","p 0.05","Diluent cotnrols","4 reps","USEPA methods, some acceptability criteria reported (water quality).","7- to 14-d old","100b media (recipe provided)","static renewal, 3-4 day renewals","Measured throughout. Measured values were within 3.5% of nominal.","","","",0.05,"8.1. Measured throughout but not reported.","Measured throughout and in acute test, but not reported for chronic. Hardness in acute test (same media) was 106 mg CaCO3/L","","",">6.6 mg/L across all treatments.","Measured but not reported. Stated as 22°C.","Controls were measured. Values not given, but refers to report not publically available."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","22-3"]
["Scores",10,5,5,5,5,5,5,5,5,4,4,0,4,4,4,1,1,0,0,3,1,3,79,0.79,""]
["Answers","32 d","Mortality","NOEC","p 0.05","Diluent cotnrols","4 reps","ASTM methods, control, water quality criteria stated.","Embryos, 14hpf","100a media (recipe provided)","static renewal, 3 day renewals","Measured throughout. Measured values were within 2.7% of nominal.","","","",0.05,"8.0. Measured throughout but not reported.","Measured throughout and in acute test, but not reported for chronic. Hardness in acute test (same media) was 91 mg CaCO3/L","","",">5 mg/L across all treatments.","Measured but not reported. Stated as 25°C. Measured in acite test is 24.7°C","Controls were measured. Values not given, but refers to report not publically available."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","24-1"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,85,0.85,""]
["Answers","32 d","Survival.","NOEC","p 0.05","Diluent controls","four replicates","Discussion of temperature being outside limits but not affecting results. Also unnacceptable mortality in Controls after operator error, therefore this data was not used in stats. So only two replicates in Controls.","<24h pf","de-chlorinated Lake Michigan water","Flow through","Measured throughout.","","","",0.05,"8.1-8.3","112-132 mg/L CaCO3","99-152 mg/L CaCO3","","7.2-7.6 mg/L","23.5-26.5","Control measured mean 0 mg/L"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","24-2"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,85,0.85,""]
["Answers","32 d","Survival.","LC",10,"Diluent controls","four replicates","Discussion of temperature being outside limits but not affecting results. Also unnacceptable mortality in Controls after operator error, therefore this data was not used in stats. So only two replicates in Controls.","<24h pf","de-chlorinated Lake Michigan water","Flow through","Measured throughout.","","","","95% CLs 9.6-16 mg/L","8.1-8.3","112-132 mg/L CaCO3","99-152 mg/L CaCO3","","7.2-7.6 mg/L","23.5-26.5","Control measured mean 0 mg/L"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","24-3"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,85,0.85,""]
["Answers","32 d","Growth (dry weight), no methods.","NOEC","p 0.05","Diluent controls","four replicates","Discussion of temperature being outside limits but not affecting results. Also unnacceptable mortality in Controls after operator error, therefore this data was not used in stats. So only two replicates in Controls.","<24h pf","de-chlorinated Lake Michigan water","Flow through","Measured throughout.","","","",0.05,"8.1-8.3","112-132 mg/L CaCO3","99-152 mg/L CaCO3","","7.2-7.6 mg/L","23.5-26.5","Control measured mean 0 mg/L"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","24-4"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,85,0.85,""]
["Answers","32 d","Growth (dry weight), no methods.","EC",10,"Diluent controls","four replicates","Discussion of temperature being outside limits but not affecting results. Also unnacceptable mortality in Controls after operator error, therefore this data was not used in stats. So only two replicates in Controls.","<24h pf","de-chlorinated Lake Michigan water","Flow through","Measured throughout.","","","","95% CLs 8.6-56 mg/L","8.1-8.3","112-132 mg/L CaCO3","99-152 mg/L CaCO3","","7.2-7.6 mg/L","23.5-26.5","Control measured mean 0 mg/L"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-14"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,4,3,3,0,0,3,3,3,77,0.77,""]
["Answers","7 d","Mortality","LC",10,"Diluent controls","Not stated although likely.","99% survival in controls","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","","","","7.9 ± 0.1","54.4 ± 4.2 mg/L CaCO3","","","7.4 ± 0 mg/L","24.8 ± 0.3","Controls measured. <det limit."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-15"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,4,3,3,0,0,3,3,3,77,0.77,""]
["Answers","7 d","Mortality","LC",10,"Diluent controls","Not stated although likely.","99% survival in controls","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","","","","7.6 ± 0.1","207.5 ± 10 mg/L CaCO3","","","7.5 ± 0 mg/L","24.8 ± 0.3","Controls measured. <det limit."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-16"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,4,3,3,0,0,3,3,3,77,0.77,""]
["Answers","7 d","Mortality","LC",10,"Diluent controls","Not stated although likely.","99% survival in controls","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","","","","7.5 ± 0.2","46.2 ± 1.8 mg/L CaCO3","","","7.5 ± 0.2 mg/L","27 ± 0.4","Controls measured. <det limit."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","4-17"]
["Scores",10,5,5,5,5,0,2,5,5,4,4,0,4,4,4,3,3,0,0,3,3,3,77,0.77,""]
["Answers","7 d","Mortality","LC",10,"Diluent controls","Not stated although likely.","99% survival in controls","Embryo from fertilsiation to 4d post fert","Reconstituted water","Flow through","Measured throughout","","","","","8.1 ± 0.1","194.8 ± 16","","","7.5 ± 0.2 mg/L","27 ± 0.4","Controls measured. <det limit."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","27-1"]
["Scores",10,10,5,5,5,5,2,5,5,0,2,0,4,4,4,3,3,3,0,0,1,3,79,0.79,""]
["Answers","7 d","Reproduction - mean number of young per adult","NOEC","p 0.05","Diluent Controls","10 replicates","Mount and Norberg Method 1984","<24 h old","Standard soft water EPA 1985 with final pH 7.4 ± 0.2","Assume static","Stock concentrations of 32 and 180 mg/L were analysed: 34 and 186 mg/L.","","","",0.05,"measured and reported for dilution water (7.4), but for test waters report states that pH rnaged from 7.5-8.5 across all tests.","44 mg CaCO3/L","32.5 mg/L CaCO3","","","25°C","Dilution water measured for B = 0 mg/L."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","27-2"]
["Scores",10,10,5,5,5,5,2,5,5,0,2,0,4,4,4,3,3,3,0,0,1,3,79,0.79,""]
["Answers","7 d","Reproduction - mean number of young per adult","MATC","p 0.05","Diluent Controls","10 replicates","Mount and Norberg Method 1984","<24 h old","Standard soft water EPA 1985 with final pH 7.4 ± 0.2","Assume static","Stock concentrations of 32 and 180 mg/L were analysed: 34 and 186 mg/L.","","","",0.05,"measured and reported for dilution water (7.4), but for test waters report states that pH rnaged from 7.5-8.5 across all tests.","44 mg CaCO3/L","32.5 mg/L CaCO3","","","25°C","Dilution water measured for B = 0 mg/L."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","27-3"]
["Scores",10,10,5,5,5,5,2,5,5,0,2,0,4,4,4,3,3,3,0,0,1,3,79,0.79,""]
["Answers","14 d","Reproduction - mean number of young per adult","NOEC","p 0.05","Diluent Controls","10 replicates","Mount and Norberg 1984","<24 h old","Standard soft water EPA 1985 with final pH 7.4 ± 0.2","Assume static","Stock concentrations of 32 and 180 mg/L were analysed: 34 and 186 mg/L.","","","",0.05,"measured and reported for dilution water (7.4), but for test waters report states that pH rnaged from 7.5-8.5 across all tests.","44 mg CaCO3/L","32.5 mg/L CaCO3","","","25°C","Dilution water measured for B = 0 mg/L."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","27-4"]
["Scores",10,10,5,5,5,5,2,5,5,0,2,0,4,4,4,3,3,3,0,0,1,3,79,0.79,""]
["Answers","14 d","Reproduction - mean number of young per adult","MATC","p 0.05","Diluent Controls","10 replicates","Mount and Norberg 1984","<24 h old","Standard soft water EPA 1985 with final pH 7.4 ± 0.2","Assume static","Stock concentrations of 32 and 180 mg/L were analysed: 34 and 186 mg/L.","","","",0.05,"measured and reported for dilution water (7.4), but for test waters report states that pH rnaged from 7.5-8.5 across all tests.","44 mg CaCO3/L","32.5 mg/L CaCO3","","","25°C","Dilution water measured for B = 0 mg/L."]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","31-5"]
["Scores",10,5,5,5,5,5,5,0,5,4,2,4,4,4,0,3,3,3,0,3,1,3,79,0.79,""]
["Answers","6-8 days embryo development, 4 days post hatch = 12 days. Stats shows 12-d exposure.","Survival","EC",10,"River water controls and DMW controls","four replicates","","Embryo stated - but not how soon post fertilisation.","Condomine River Water","Static (in brochure in Appendix)","","Cu","","","95% CLs quoted as not being reliable","6.9-7.5","81 mg/L","89 mg/L","","104-121% saturation","25 ± 1°C","Boric acid, Diluent control had 45µg/L B"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","31-5"]
["Scores",10,5,5,5,5,5,5,0,5,4,2,4,4,4,4,3,3,3,0,3,1,3,83,0.83,""]
["Answers","6-8 days embryo development, 4 days post hatch = 12 days. Stats shows 12-d exposure.","Survival","NOEC","p 0.05","River water controls and DMW controls","four replicates","","Embryo stated - but not how soon post fertilisation.","Condomine River Water","Static (in brochure in Appendix)","","Cu","","","p 0.05","6.9-7.5","81 mg/L","89 mg/L","","104-121% saturation","25 ± 1°C","Boric acid, Diluent control had 45µg/L B"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","32-1"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,85,0.85,""]
["Answers","50 d","Survival","NOEC","p<0.05","","triplicate","100% survival in controls and physico-chemical parameters deemed within acceptable range for the species by the author ","Advanced fry, 2.60 ± 0.14 g","Mix of pond water and tap water in ratio 1:3","Static renewal. Half test solution renewed on laternate days","Measured on laternate days in the test","","","",0.05,"7.4 ± 0.1","114 ± 6 mg/L CaCO3","143 ± 17 mg/L CaCO3","","6.52 ± 0.68 mg/L","24.2 ± 2.8°C in text, but in Table 26 ± 3","AR grade salts, and all solutions measured. 0.01 mg/L B found in controls"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","32-2"]
["Scores",10,5,5,5,5,5,2,5,5,4,4,0,4,4,4,3,3,3,0,3,3,3,85,0.85,""]
["Answers","50 d","Survival","NOEC","p<0.05","","triplicate","100% survival in controls and physico-chemical parameters deemed within acceptable range for the species by the author ","Advanced fry, 2.60 ± 0.14 g","Mix of pond water and tap water in ratio 1:3","Static renewal. Half test solution renewed on laternate days","Measured on laternate days in the test","","","",0.05,"7.4 ± 0.1","114 ± 6 mg/L CaCO3","143 ± 17 mg/L CaCO3","","6.52 ± 0.68 mg/L","24.2 ± 2.8°C in text, but in Table 26 ± 3","AR grade salts, and all solutions measured. 0.01 mg/L B found in controls"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","33-1"]
["Scores",10,10,0,5,5,5,0,5,5,4,4,0,0,4,4,1,1,0,0,0,1,0,64,0.64,""]
["Answers","total 25 days exposure. Eggs started hatching from 17d.","Description of deformity: Bodies of normal larvae were nearly perfectly linear while motionless, but deformed larvae displayed\na crescent-shaped body pattern. This curling defect was most noticeable when the larvae began freely swimming. Normal larvae of these species moved linearly through the\nwater using serpentine tail movements to propel themselves rapidly in a given direction. Curled larvae moved in a helical pattern, rather than linear, at greatly reduced speeds.","","statistically significant difference from Control at p = 0.001","","5 replicates","","Eggs at blastula stage","recipe for synthetic water given.","not stated, but from description = static.","Measured beginning and end.","","Only two B concentrations were tested.","","p = 0.001",6.5,"Calculated from Mg and Ca recipe = 137 mg/L CaCO3","","","","Stated as 5°C for 5 days, then increased incrementally to 10 °C for rest of exposure.","Not stated"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","33-2"]
["Scores",10,10,0,5,5,5,0,5,5,4,4,0,0,4,4,1,1,0,0,0,1,0,64,0.64,""]
["Answers","total 23 days exposure. Eggs started hatching from 13d.","Description of deformity: Bodies of normal larvae were nearly perfectly linear while motionless, but deformed larvae displayed\na crescent-shaped body pattern. This curling defect was most noticeable when the larvae began freely swimming. Normal larvae of these species moved linearly through the\nwater using serpentine tail movements to propel themselves rapidly in a given direction. Curled larvae moved in a helical pattern, rather than linear, at greatly reduced speeds.","","statistically significant difference from Control at p = 0.001","","5 replicates","","Eggs at blastula stage","recipe for synthetic water given.","not stated, but from description = static.","Measured beginning and end.","","Only two B concentrations were tested.","","p = 0.001",6.5,"Calculated from Mg and Ca recipe = 137 mg/L CaCO3","","","","Stated as 5°C for 5 days, then increased incrementally to 10 °C for rest of exposure.","Not stated"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","33-3"]
["Scores",10,10,0,5,5,5,0,5,5,4,4,0,0,4,4,1,1,0,0,0,1,0,64,0.64,""]
["Answers","total 44 days exposure. Eggs started hatching from 38d.","Description of deformity: Deformities in A. maculatum larvae were quite different than\nthe curling defect in the other two species. Spotted salamander larvae from high boron treatments showed a swelling of the thoracic region and enlarged, shortened gills. These larvae displayed greatly reduced movements compared to normal larvae, and were only induced to move after a great deal of mechanical stimulation.","","statistically significant difference from Control at p = 0.001","","5 replicates","","Eggs at blastula stage","recipe for synthetic water given.","not stated, but from description = static.","Measured beginning and end.","","Only two B concentrations were tested.","","p = 0.001",6.5,"Calculated from Mg and Ca recipe = 137 mg/L CaCO3","","","","Stated as 5°C for 5 days, then increased incrementally to 10 °C for rest of exposure.","Not stated"]
["Category","Freshwater/metal/non- plant","Paper and data unique identifier(eg. 1-1,10-150)","33-4"]
["Scores",10,10,0,5,5,5,0,5,5,4,4,0,0,4,4,1,1,0,0,0,1,0,64,0.64,""]
["Answers","total 23 days exposure. Eggs started hatching from 15d.","Number of hatched larvae","","statistically significant difference from Control at p = 0.001","","5 replicates","","Eggs at blastula stage","recipe for synthetic water given.","not stated, but from description = static.","Measured beginning and end.","","Only two B concentrations were tested.","","p = 0.001",6.5,"Calculated from Mg and Ca recipe = 137 mg/L CaCO3","","","","Stated as 5°C for 5 days, then increased incrementally to 10 °C for rest of exposure.","Not stated"]

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[null,1,"Fill in the blank cells below, based on information provided in the paper being assessed. You can either pick an option from the dropdown list or add the appropriate detail using the 'Add to list' button. ",null,null,null,null,null,null,null,null,null,null,null,"* All the text in RED are used for drop down list ,make changes with caution."]
[null,null,"Category",{"$f":"IF(AF3=\"\",0,1)","v":0},"Freshwater/metal/non- plant",{"$f":"IF(E3=\"\",0,1)","v":1},"Citation No.",{"$f":"IF(ISERROR(FIND(\"-\",E3)),E3,LEFT(E3,FIND(\"-\",E3)-1))","v":"34"},null,"Taxonomic group",null,null,null,null,"Species",null,null,"Lifestage",null,null,"Exposure duration",null,null,"Measure of toxicity",null,null,"Toxicity endpoint",null,null,"Taxonomic group",null,null,"Phylum",null,null,"Class",null,null,"Media",null,null,"Temperature ",null,null,"Acute Or Chronic",null,null,"PH",null,null,"Salinity/Comdictivity"]
[null,null,"Paper and data unique identifier(eg. 1-1,10-150)",{"$f":"IF(AI3=\"\",0,1)","v":0},"34-1",{"$f":"IF(E4=\"\",0,1)","v":1},"Species (Scientific name)",null,null,"Phylum",null,null,null,null,"Aedes albopictus",22,{"$f":"IF(P3=0,\"\",INDEX(O3:O111,P3))","v":"Oncorhynchus kisutch"},"1 month",0,{"$f":"IF(S3=0,\"\",INDEX(R3:R111,S3))","v":""},23,0,{"$f":"IF(V3=0,\"\",INDEX(U3:U111,V3))","v":""},"42 d",0,{"$f":"IF(Y3=0,\"\",INDEX(X3:X111,Y3))","v":""},"Biomass (dry weight)",0,{"$f":"IF(AB3=0,\"\",INDEX(AA3:AA111,AB3))","v":""},"Amphibian",0,{"$f":"IF(AE3=0,\"\",INDEX(AD3:AD111,AE3))","v":""},"Arthropoda",0,{"$f":"IF(AH3=0,\"\",INDEX(AG3:AG111,AH3))","v":""},"Actinopterygii",0,{"$f":"IF(AK3=0,\"\",INDEX(AJ3:AJ111,AK3))","v":""},"Freshwater",0,{"$f":"IF(AN3=0,\"\",INDEX(AM3:AM111,AN3))","v":""},13.7,0,{"$f":"IF(AQ3=0,\"\",INDEX(AP3:AP111,AQ3))","v":""},"Acute ",0,{"$f":"IF(AT3=0,\"\",INDEX(AS3:AS111,AT3))","v":""},6.5,0,{"$f":"IF(AW3=0,\"\",INDEX(AV3:AV111,AW3))","v":""},null,null,"N.A.",1]
[null,null,"Citation",{"$f":"IF(AL3=\"\",0,1)","v":0},"Thompson, J.A., Davis, J.C and Drew, R.E. 1976. Toxicity, uptake and survey studies of boron in the marine environment. Water Research 10, 869-875.",{"$f":"IF(H8=\"\",0,1)","v":0},"Lifestage",null,null,"Class",null,null,null,null,"Ambystoma jeffersonian",null,null,"1 year old",null,null,23,null,null,"EC10",null,null,"Biomass (length)",null,null,"Bivalve",null,null,"Chlorophyta",null,null,"Amphibia",null,null,"Marinewater",null,null,"10°C",null,null,"Chronic",null,null,7.5]
[null,null,null,{"$f":"IF(AO3=\"\",0,1)","v":0},null,{"$f":"IF(AX3=\"\",0,1)","v":0},"Exposure duration",null,null,"Media",null,null,null,null,"Ambystoma jeffersonianum",null,null,"Advanced fry",null,null,25,null,null,"IC25",null,null,"Hatching success",null,null,"Cladoceran",null,null,"Chordata",null,null,"Bivalve",null,null,"N.A.",null,null,"11 ± 0.5°C",null,null,"N.A.",null,null,7.6]
[null,null,null,{"$f":"IF(AR3=\"\",0,1)","v":0},null,{"$f":"IF(Q3=\"\",0,1)","v":1},"Measure of toxicity",null,null,"Temperature ",null,null,null,null,"Ambystonium maculatum",null,null,"alevin",null,null,44,null,null,"LC10",null,null,"Mortality",null,null,"Fish",null,null,"Mollusca",null,null,"Branchiopoda",null,null,null,null,null,"12.5°C",null,null,null,null,null,7.7]
[null,null,null,{"$f":"IF(AU3=\"\",0,1)","v":0},null,{"$f":"IF(T3=\"\",0,1)","v":0},"Toxicity endpoint",null,null,"Acute Or Chronic ",null,null,null,null,"Anaxyrus fowleri",null,null,"embryo",null,null,"10 d",null,null,"LC20",null,null,"Mortality and teratogenesis",null,null,"Macro-invertebrate",null,null,"N.A.",null,null,"Chlorophyceae",null,null,null,null,null,"13.3°C",null,null,null,null,null,7.7]
[null,null,null,null,null,{"$f":"IF(W3=\"\",0,1)","v":0},"Toxicity value",null,null,"PH",null,null,null,null,"Australian Bass",null,null,"Embryo (blastula)",null,null,"11 d",null,null,"LC50",null,null,"N.A.",null,null,"Microalga",null,null,"Tracheophyta",null,null,"Malacostraca",null,null,null,null,null,"13.7°C",null,null,null,null,null,7.8]
[null,2,"Select the appropriate answer option to each question. Comments can be added to the cells in column H.",null,null,{"$f":"IF(Z3=\"\",0,1)","v":0},null,null,null,"Salinity/Conductivity","N.A.",null,null,null,"Bufo americanus",null,null,"juvenile",null,null,"11 hour",null,null,"LOEC",null,null,"Nomral larval development",null,null,"Micro-invertebrate",null,null,null,null,null,"N.A.",null,null,null,null,null,"13°C",null,null,null,null,null,7.9]
[null,"*","If the selected answer option does not work try clicking on any other cell first and then click back on the answer option again.",null,null,{"$f":"IF(AC3=\"\",0,1)","v":0},null,null,null,null,null,null,null,null,"Bufo fowleri",null,null,"juvenile",null,null,"11.8 d",null,null,"MATC",null,null,"Normal larval development",null,null,"N.A.",null,null,null,null,null,null,null,null,null,null,null,"14°C",null,null,null,null,null,8]
[null,"No.","Question","Marks Awarded","Answer Options","Option References","Scores","Answers",null,null,null,null,null,null,"Carassius auratus",null,null,"N.A.",null,null,"12 d",null,null,"N.A.",null,null,"Reproduction",null,null,null,null,null,null,null,null,null,null,null,null,null,null,"15°C",null,null,null,null,null,8.1]
[null,null,"Was the duration of the exposure stated (e.g. 48 or 96 h)?",10,null,0,{"$f":"IF(F15=0,0,INDEX($D12:$D13,F12,1))","v":0},null,null,null,null,null,null,null,"Ceriodaphnia dubia",null,null,"neonates",null,null,"13 days",null,null,"NOAEL",null,null,"Reproduction ( number of young)",null,null,null,null,null,null,null,null,null,null,null,null,null,null,"19.3-20.9°C",null,null,null,null,null,8.1999999999999993]
[null,1,null,0,null,null,null,null,null,null,null,null,null,null,"Cirrhinus mrigala",null,null,null,null,null,"14 d",null,null,"NOEC",null,null,"Reproduction (brood size)",null,null,null,null,null,null,null,null,null,null,null,null,null,null,"19.5-20.5°C",null,null,null,null,null,8.3000000000000007]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Danio rerio",null,null,null,null,null,"21 d",null,null,null,null,null,"Specific growth rate (%.d)",null,null,null,null,null,null,null,null,null,null,null,null,null,null,"19°C",null,null,null,null,null,8.4]
[null,null,"Was the biological endpoint (e.g. immobilization or population growth) stated and defined (10 marks)? Award 5 marks if the biological endpoint is only stated.",10,null,0,{"$f":"IF(F15=0,0,INDEX($D15:$D17,F15,1))","v":0},null,null,null,null,null,null,null,"Daphnia magna",null,null,null,null,null,"28 d",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"20°C",null,null,null,null,null,8.5]
[null,2,null,5,null,null,null,null,null,null,null,null,null,null,"Hyalella azteca",null,null,null,null,null,"32 d",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"22°C",null,null,null,null,null,"6.8-7.9"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Ictalurus punctatus",null,null,null,null,null,"34 d",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"24.7°C",null,null,null,null,null,"6.9-7.5"]
[null,null,"Was the biological effect stated (e.g. LC or NOEC)?",5,null,0,{"$f":"IF(F18=0,0,INDEX($D18:$D19,F18,1))","v":0},null,null,null,null,null,null,null,"Lambriculus variegatus",null,null,null,null,null,"36 d",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"24.8-27°C",null,null,null,null,null,"7.1-8.7"]
[null,3,null,0,null,null,null,null,null,null,null,null,null,null,"Lampsilis siliquoidea",null,null,null,null,null,"42 d",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"24°C",null,null,null,null,null,"7.2-8.0"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Melanotaenia spledida",null,null,null,null,null,"50 d",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"25 ± 1°C",null,null,null,null,null,"7.3-8.2"]
[null,4,"Was the biological effect quantified (e.g. 50% effect, 25% effect)? \nNote: The effect for NOEC and LOEC data must be quantified.",5,null,0,{"$f":"IF(F21=0,0,INDEX($D21:$D22,F21,1))","v":0},null,null,null,null,null,null,null,"Micropteris salmoides",null,null,null,null,null,"7 d",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"25.3°C",null,null,null,null,null,"7.4 ± 0.1"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"N.A.",null,null,null,null,null,"7.5 d",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"25°C",null,null,null,null,null,"7.5-7.9"]
[null,5,"Were appropriate controls (e.g. a no-toxicant control and/or solvent control) used?",5,null,0,{"$f":"IF(F23=0,0,INDEX($D23:$D24,F23,1))","v":0},null,null,null,null,null,null,null,"Onchorynchus mykiss",null,null,null,null,null,"9 d",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"25°C",null,null,null,null,null,"7.5-8.5"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Oncorhynchus kisutch",null,null,null,null,null,"96 h",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"25°C",null,null,null,null,null,"8.1 ± 0.1 in Control"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Pimephales promelas",null,null,null,null,null,"N.A.",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"26 ± 3",null,null,null,null,null,"8.1-8.3"]
[null,6,"Was each control and contaminant concentration at least duplicated?",5,null,0,{"$f":"IF(F26=0,0,INDEX($D26:$D27,F26,1))","v":0},null,null,null,null,null,null,null,"Rana pipiens",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"29.4°C",null,null,null,null,null,"N.A."]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Rana sylvatica",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"5-10°C",null,null,null,null,null,"PH11"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Selenastrum carpicornutum",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"N.A.",null,null,null,null,null,"PH7.0"]
[null,7,"Were test acceptability criteria stated (e.g. mortality in controls must not exceed a certain percentage) (5 marks)?\nOR Were test acceptability criteria inferred (e.g. test method used [USEPA, OECD, ASTM etc.]) (award 2 marks). \nNote: Data that fail the acceptability criteria are automatically deemed to be of unacceptable quality and must not be used. ",5,null,0,{"$f":"IF(F29=0,0,INDEX($D29:$D31,F29,1))","v":0}]
[null,null,null,2]
[null,null,null,0]
[null,8,"Were the characteristics of the test organism (e.g. length, mass, age) stated?",5,null,0,{"$f":"IF(F32=0,0,INDEX($D32:$D34,F32,1))","v":0}]
[null,null,null,0]
[]
[null,9,"Was the type of test media used stated?",5,null,0,{"$f":"IF(F35=0,0,INDEX($D35:$D37,F35,1))","v":0}]
[null,null,null,0]
[]
[null,null,"Was the type of exposure (e.g. static, flow through) stated? ",4,null,0,{"$f":"IF(F38=0,0,INDEX($D38:$D40,F38,1))","v":0}]
[null,10,null,0]
[]
[null,null,"Were the contaminant concentrations measured at the beginning and end of the exposure (4 marks)? Award 2 marks if they were measured only once during the test.\nNote: If the concentrations were not measured then automatically the data cannot be high quality. ",4,null,0,{"$f":"IF(F41=0,0,INDEX($D41:$D43,F41,1))","v":0}]
[null,11,null,2]
[null,null,null,0]
[null,null,"Were parallel reference toxicant toxicity tests conducted?",4,null,0,{"$f":"IF(F44=0,0,INDEX($D44:$D46,F44,1))","v":0}]
[null,12,null,0]
[]
[null,null,"Was there a concentration–response relationship either observable or stated?",4,null,0,{"$f":"IF(F47=0,0,INDEX($D47:$D49,F47,1))","v":0}]
[null,13,null,0]
[]
[null,null,"Was an appropriate statistical method or model used to determine the toxicity?\nNote: They should be accepted by a recognised national or international regulatory body (e.g. USEPA, OECD, ASTM). ",4,null,0,{"$f":"IF(F50=0,0,INDEX($D50:$D52,F50,1))","v":0}]
[null,14,null,0]
[]
[null,15,"For LC/EC/BEC data was an estimate of variability provided? \nOR \nFor NO EC/LOEC data was the significance level 0.05 or less?  \n\n",4,null,0,{"$f":"IF(F53=0,0,INDEX($D53:$D55,F53,1))","v":0}]
[null,null,null,0]
[]
[null,16,"Were the following parameters measured and stated? (3 marks if measured and stated, 1 mark if just measured)."]
[null,null,null,3,null,0,{"$f":"IF(F57=0,0,INDEX($D57:$D59,F57,1))","v":0}]
[null,16.100000000000001,"pH",1]
[null,null,null,0]
[null,null,null,3,null,0,{"$f":"IF(F60=0,0,INDEX($D60:$D62,F60,1))","v":0}]
[null,16.2,"Hardness",1]
[null,null,null,0]
[null,null,null,3,null,0,{"$f":"IF(F63=0,0,INDEX($D63:$D65,F63,1))","v":0}]
[null,16.3,"Alkalinity",1]
[null,null,null,0]
[null,null,null,3,null,0,{"$f":"IF(F66=0,0,INDEX($D66:$D68,F66,1))","v":0}]
[null,16.399999999999999,"Organic carbon concentration",1]
[null,null,null,0]
[null,null,null,3,null,0,{"$f":"IF(F69=0,0,INDEX($D69:$D71,F69,1))","v":0}]
[null,16.5,"Dissolved Oxygen",1]
[null,null,null,0]
[null,null,"Was the temperature measured and stated (3 marks)? Award 1 mark if the temperature was measured but not stated or if only the temperature settings of the room or chamber are stated.",3,null,0,{"$f":"IF(F72=0,0,INDEX($D72:$D74,F72,1))","v":0}]
[null,17,null,1]
[null,null,null,0]
[null,null,"Were test solutions, blanks and/or controls tested for contamination OR were analytical reagent grade chemicals used or the highest possible purity chemicals used for the experiment? (3 marks).",3,null,0,{"$f":"IF(F75=0,0,INDEX($D75:$D76,F75,1))","v":0}]
[null,18,null,0]
[]
[null,null,null,null,null,null,null,null,{"$f":"(COUNTIF(F12:F76,\"=0\"))+COUNTIF(F2:F10,\"=0\")+COUNTIF(D2:D7,\"=0\")","v":34}]
[]
[]
[null,null,"Total score",null,null,null,{"$f":"IF(SUM(G12:G75)=0,\"\",SUM(G12:G75))","v":""}]
[null,null,"Total Max. possible score for the various types of data and contaminants ",null,{"$f":"D12+D15+D18+D21+D23+D26+D29+D32+D35+D38+D41+D44+D47+D50+D53+D57+D66+D69+D72+D75+D63+D60","v":100}]
[null,null,"Quality Score",null,{"$f":"\"= [Total score / Total possible score] * 100 = \"","v":"= [Total score / Total possible score] * 100 = "},null,{"$f":"IF(ISERROR(G81/E82),#N/A,G81/E82)","v":{"$error":"#N/A"}},{"$f":"IF(F41=3,\"The highest possible grade for this datum is acceptable.\",\"\")","v":""}]
[]
[null,null,"H = high quality                           Quality score ≥ 80%     "]
[null,null,"A = acceptable quality         > 50% Quality score < 80%  "]
[null,null,"U = unacceptable quality              Quality score ≤ 50% "]
[]
[3,null,"Click the 'Save & Update' button to save and export the answers to the 'All results' worksheet. "]
[null,null,"Select the 'Reset Answers' button if you wish to clear all answers."]

{"sheet":"FW metal plant","index":7,"n_rows":87,"n_cols":53,"rows_kept":87,"cut":false,"hidden":false,"merged":[[71,2,74,2],[71,7,74,7],[52,2,54,2],[52,7,54,7],[68,2,70,2],[68,7,70,7],[65,7,67,7],[56,7,58,7],[59,7,61,7],[62,7,64,7],[43,2,45,2],[43,7,45,7],[46,2,48,2],[46,7,48,7],[49,2,51,2],[49,7,51,7],[34,2,36,2],[34,7,36,7],[37,2,39,2],[37,7,39,7],[40,2,42,2],[40,7,42,7],[25,2,27,2],[25,7,27,7],[28,2,30,2],[28,7,30,7],[31,2,33,2],[31,7,33,7],[17,2,19,2],[17,7,19,7],[20,2,21,2],[20,7,21,7],[22,2,24,2],[22,7,24,7],[3,4,7,4],[9,2,9,4],[11,2,13,2],[11,7,13,7],[14,2,16,2],[14,7,16,7]]}
[null,1,"Fill in the blank cells below, based on information provided in the paper being assessed. You can either pick an option from the dropdown list or add the appropriate detail using the 'Add to list' button. ",null,null,null,null,null,null,null,null,null,null,null,"* All the text in RED are used for drop down list ,make changes with caution."]
[null,null,"Category",{"$f":"IF(AF3=\"\",0,1)","v":1},"Freshwater/metal/plant",{"$f":"IF(E3=\"\",0,1)","v":1},"Citation No.",{"$f":"IF(ISERROR(FIND(\"-\",E3)),E3,LEFT(E3,FIND(\"-\",E3)-1))","v":"26"},null,"Taxonomic group",null,null,null,null,"Species",null,null,"Lifestage",null,null,"Exposure duration",null,null,"Measure of toxicity",null,null,"Toxicity endpoint",null,null,"Taxonomic group",null,null,"Phylum",null,null,"Class",null,null,"Media",null,null,"Temperature ",null,null,"Acute Or Chronic",null,null,"PH",null,null,"Salinity/Comdictivity"]
[null,null,"Paper and data unique identifier(eg. 1-1,10-150)",{"$f":"IF(AI3=\"\",0,1)","v":1},"26-29",{"$f":"IF(E4=\"\",0,1)","v":1},"Species (Scientific name)",null,null,"Phylum",null,null,null,null,"Aedes albopictus",26,{"$f":"IF(P3=0,\"\",INDEX(O3:O108,P3))","v":"Nostoc punctiforme 2"},"1 month",9,{"$f":"IF(S3=0,\"\",INDEX(R3:R108,S3))","v":"N.R."},"10 d",31,{"$f":"IF(V3=0,\"\",INDEX(U3:U108,V3))","v":"6-26d"},"EC10",9,{"$f":"IF(Y3=0,\"\",INDEX(X3:X108,Y3))","v":"LOEC"},"Abnormality",9,{"$f":"IF(AB3=0,\"\",INDEX(AA3:AA108,AB3))","v":"Growth rate (Chl a)"},"Bacteria",3,{"$f":"IF(AE3=0,\"\",INDEX(AD3:AD108,AE3))","v":"Blue-green algae (Eubacteria)"},"Angiosperm",5,{"$f":"IF(AH3=0,\"\",INDEX(AG3:AG108,AH3))","v":"Cyanobacteria"},"Bacillariophyceae ",5,{"$f":"IF(AK3=0,\"\",INDEX(AJ3:AJ108,AK3))","v":"Cyanophyceae"},"Freshwater",1,{"$f":"IF(AN3=0,\"\",INDEX(AM3:AM108,AN3))","v":"Freshwater"},"13h 35°C, 11h 10°C",4,{"$f":"IF(AQ3=0,\"\",INDEX(AP3:AP108,AQ3))","v":"20°C"},"Acute ",2,{"$f":"IF(AT3=0,\"\",INDEX(AS3:AS108,AT3))","v":"Chronic"},6,12,{"$f":"IF(AW3=0,\"\",INDEX(AV3:AV108,AW3))","v":"N.R."},"Salinity1",null,"N.A."]
[null,null,"Citation",{"$f":"IF(AL3=\"\",0,1)","v":1},"V W (1985). Boron tolerance by freshwater phytoplankton. Division of Marine and Freshwater Science, Taupo Research Laboratory.",{"$f":"IF(H8=\"\",0,1)","v":1},"Lifestage",null,null,"Class",null,null,null,null,"Anabaena flos-aquae",null,null,"1 year old",null,null,"11 d",null,null,"EC50",null,null,"biomass (dry weight)",null,null,"Blue-green algae",null,null,"Bacillariophyta",null,null,"Branchiopoda",null,null,"Marinewater",null,null,"15°C",null,null,"Chronic",null,null,6.8,null,null,"Salinity2"]
[null,null,null,{"$f":"IF(AO3=\"\",0,1)","v":1},null,{"$f":"IF(AX3=\"\",0,1)","v":1},"Exposure duration",null,null,"Media",null,null,null,null,"Anabaena oscillarioides",null,null,"3-frond clones",null,null,"11 d",null,null,"IC10",null,null,"biomass (particle volume)",null,null,"Blue-green algae (Eubacteria)",null,null,"Charophyta",null,null,"Charophyceae",null,null,"N.A.",null,null,"18.5-21.4°C",null,null,"N.A.",null,null,"5.2-5.8"]
[null,null,null,{"$f":"IF(AR3=\"\",0,1)","v":1},null,{"$f":"IF(Q3=\"\",0,1)","v":1},"Measure of toxicity",null,null,"Temperature ",null,null,null,null,"Anabaena oscillarioides 1",null,null,"apical stem cutting",null,null,"11 hour",null,null,"LC50",null,null,"frond production",null,null,"Fish ",null,null,"Chlorophyta",null,null,"Chlorophyceae",null,null,null,null,null,"20°C",null,null,null,null,null,"6.2-6.4"]
[null,null,null,{"$f":"IF(AU3=\"\",0,1)","v":1},null,{"$f":"IF(T3=\"\",0,1)","v":1},"Toxicity endpoint",null,null,"Acute Or Chronic ",null,null,null,null,"Anabaena oscillarioides 2",null,null,"exponentially growing cells",null,null,"12 d",null,null,"MATC",null,null,"Germination",null,null,"Macrophyte",null,null,"Cyanobacteria",null,null,"Cyanophyceae",null,null,null,null,null,"22°C",null,null,null,null,null,"6.8-7.7"]
[null,null,null,null,null,{"$f":"IF(W3=\"\",0,1)","v":1},"Toxicity value",1,null,"PH",null,null,null,null,"Anacystis nidulans",null,null,"frond",null,null,"13 days",null,null,"N.A.",null,null,"Growth",null,null,"Microalga",null,null,"N.A.",null,null,"Mediophyceae",null,null,null,null,null,"23°C",null,null,null,null,null,"7.0-7.2"]
[null,2,"Select the appropriate answer option to each question. Comments can be added to the cells in column H.",null,null,{"$f":"IF(Z3=\"\",0,1)","v":1},null,null,null,"Salinity/Conductivity","N.A.",null,null,null,"Australian Bass",null,null,"late log phase",null,null,"14 d",null,null,"NEC",null,null,"Growth (rate)",null,null,"N.A.",null,null,"Ochrophyta",null,null,"Monocot",null,null,null,null,null,"24°C",null,null,null,null,null,"7.5-8.3"]
[null,"*","If the selected answer option does not work try clicking on any other cell first and then click back on the answer option again.",null,null,{"$f":"IF(AC3=\"\",0,1)","v":1},null,null,null,null,null,null,null,null,"Chara corallina",null,null,"N.A.",null,null,"16 d",null,null,"NOEC",null,null,"Growth (yield)",null,null,null,null,null,"Tracheophyta",null,null,"N.A.",null,null,null,null,null,"25°C",null,null,null,null,null,"7.5-8.5"]
[null,"No.","Question","Marks Awarded","Answer Options","Option References","Scores","Answers",null,null,null,null,null,null,"Chlorella pyrenoidosa",null,null,"N.R.",null,null,"18 d",null,null,"LOEC",null,null,"Growth rate (Chl a)",null,null,null,null,null,null,null,null,null,null,null,null,null,null,"25-29°C",null,null,null,null,null,"7.7-10.1"]
[null,null,"Was the duration of the exposure stated (e.g. 48 or 96 h)?",10,null,2,{"$f":"IF(F15=0,0,INDEX($D12:$D13,F12,1))","v":0},"Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.",null,null,null,null,null,null,"Chlorella vulgaris",null,null,"Seed",null,null,"20 d",null,null,null,null,null,"Growth, yield (Chl a)",null,null,null,null,null,null,null,null,null,null,null,null,null,null,"26°C",null,null,null,null,null,"7.7-8.1"]
[null,1,null,0,null,null,null,null,null,null,null,null,null,null,"Cyclotella sp",null,null,null,null,null,"21 d",null,null,null,null,null,"Mortality",null,null,null,null,null,null,null,null,null,null,null,null,null,null,"N.A.",null,null,null,null,null,"N.A."]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Egeria densa",null,null,null,null,null,"22 d",null,null,null,null,null,"N.A.",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"N.R."]
[null,null,"Was the biological endpoint (e.g., immobilization or population growth) stated and defined (10 marks)? Award 5 marks if the biological endpoint is only stated.",10,null,1,{"$f":"IF(F15=0,0,INDEX($D15:$D17,F15,1))","v":10},"Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.",null,null,null,null,null,null,"Eichhornia crassipes",null,null,null,null,null,"24 d",null,null,null,null,null,"Necrosis",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"PH11"]
[null,2,null,5,null,null,null,null,null,null,null,null,null,null,"Lemna disperma",null,null,null,null,null,"26 d",null,null,null,null,null,"Node number",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"PH7.0 "]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Lemna gibba",null,null,null,null,null,"28 d",null,null,null,null,null,"Plant condition"]
[null,null,"Was the biological effect stated (e.g. LC or NOEC)?",5,null,1,{"$f":"IF(F18=0,0,INDEX($D18:$D19,F18,1))","v":5},"LOEC from text and statistics",null,null,null,null,null,null,"Lemna minor",null,null,null,null,null,"30 days",null,null,null,null,null,"Reproduction"]
[null,3,null,0,null,null,null,null,null,null,null,null,null,null,"Melosira granulata",null,null,null,null,null,"4-7 d",null,null,null,null,null,"Shoot growth"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Microcystis aeruginosa",null,null,null,null,null,"7 d",null,null,null,null,null,"Shoot weight"]
[null,4,"Was the biological effect quantified (e.g. 50% effect, 25% effect)? \nNote: The effect for NOEC and LOEC data must be quantified.",5,null,1,{"$f":"IF(F21=0,0,INDEX($D21:$D22,F21,1))","v":5},"p < 0.05",null,null,null,null,null,null,"N.A.",null,null,null,null,null,"7 d"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Navicula sp 1.",null,null,null,null,null,"7 d"]
[null,5,"Were appropriate controls (e.g. a no-toxicant control and/or solvent control) used?",5,null,1,{"$f":"IF(F23=0,0,INDEX($D23:$D24,F23,1))","v":5},"non-toxicant controls",null,null,null,null,null,null,"Navicula sp. 2",null,null,null,null,null,"7 d"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Nostoc muscorum",null,null,null,null,null,"72 h"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Nostoc muscorum 1",null,null,null,null,null,"8 d"]
[null,6,"Was each control and contaminant concentration at least duplicated?",5,null,1,{"$f":"IF(F26=0,0,INDEX($D26:$D27,F26,1))","v":5},"5 replicates",null,null,null,null,null,null,"Nostoc muscorum 2",null,null,null,null,null,"9 d"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Nostoc punctiforme 1",null,null,null,null,null,"90-120 d"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,"Nostoc punctiforme 2",null,null,null,null,null,"96 h"]
[null,7,"Were test acceptability criteria stated (e.g. mortality in controls must not exceed a certain percentage) (5 marks)?\nOR Were test acceptability criteria inferred (e.g. test method used [USEPA, OECD, ASTM etc.]) (award 2 marks). \nNote: Data that fail the acceptability criteria are automatically deemed to be of unacceptable quality and must not be used. ",5,null,3,{"$f":"IF(F29=0,0,INDEX($D29:$D31,F29,1))","v":0},null,null,null,null,null,null,null,"Potamogeton ochreatus",null,null,null,null,null,"N.A."]
[null,null,null,2,null,null,null,null,null,null,null,null,null,null,"Selenastrum capricornutum",null,null,null,null,null,"4-36d"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,"Spirodella polyrrhiza",null,null,null,null,null,"4-12 d"]
[null,8,"Were the characteristics of the test organism (e.g. length, mass, age) stated?",5,null,1,{"$f":"IF(F32=0,0,INDEX($D32:$D34,F32,1))","v":5},"1 x 10^5 cells/mL",null,null,null,null,null,null,"Synedra sp.",null,null,null,null,null,"4-18 d"]
[null,null,null,0,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"6-26d"]
[]
[null,9,"Was the type of test media used stated?",5,null,1,{"$f":"IF(F35=0,0,INDEX($D35:$D37,F35,1))","v":5},"1/10th Woods Hole Medium"]
[null,null,null,0]
[]
[null,null,"Was the type of exposure (e.g. static, flow through) stated? ",4,null,2,{"$f":"IF(F38=0,0,INDEX($D38:$D40,F38,1))","v":0}]
[null,10,null,0]
[]
[null,null,"Were the contaminant concentrations measured at the beginning and end of the exposure (4 marks)? Award 2 marks if they were measured only once during the test.\nNote: If the concentrations were not measured then automatically the data cannot be high quality. ",4,null,3,{"$f":"IF(F41=0,0,INDEX($D41:$D43,F41,1))","v":0},"Scored 0 because although concs were measured beginning and end, these were not reported anywhere."]
[null,11,null,2]
[null,null,null,0]
[null,null,"Were parallel reference toxicant toxicity tests conducted?",4,null,2,{"$f":"IF(F44=0,0,INDEX($D44:$D46,F44,1))","v":0}]
[null,12,null,0]
[]
[null,null,"Was there a concentration–response relationship either observable or stated?",4,null,1,{"$f":"IF(F47=0,0,INDEX($D47:$D49,F47,1))","v":4}]
[null,13,null,0]
[]
[null,null,"Was an appropriate statistical method or model used to determine the toxicity?\nNote: They should be accepted by a recognised national or international regulatory body (e.g. USEPA, OECD, ASTM). ",4,null,1,{"$f":"IF(F50=0,0,INDEX($D50:$D52,F50,1))","v":4}]
[null,14,null,0]
[]
[null,15,"For LC/EC/BEC data was an estimate of variability provided? \nOR \nFor NO EC/LOEC data was the significance level 0.05 or less?  \n\n",4,null,1,{"$f":"IF(F53=0,0,INDEX($D53:$D55,F53,1))","v":4},"p 0.05"]
[null,null,null,0]
[]
[null,16,"Were the following parameters measured and stated? (3 marks if measured and stated, 1 mark if just measured)."]
[null,null,null,3,null,3,{"$f":"IF(F57=0,0,INDEX($D57:$D59,F57,1))","v":0}]
[null,16.100000000000001,"pH ",1]
[null,null,null,0]
[null,null,null,3,null,3,{"$f":"IF(F60=0,0,INDEX($D60:$D62,F60,1))","v":0}]
[null,16.2,"Hardness",1]
[null,null,null,0]
[null,null,null,3,null,3,{"$f":"IF(F63=0,0,INDEX($D63:$D65,F63,1))","v":0}]
[null,16.3,"Alkalinity ",1]
[null,null,null,0]
[null,null,null,3,null,3,{"$f":"IF(F66=0,0,INDEX($D66:$D68,F66,1))","v":0}]
[null,16.399999999999999,"Organic carbon concentration",1]
[null,null,null,0]
[null,null,"Was the temperature measured and stated (3 marks)? Award 1 mark if the temperature was measured but not stated or if only the temperature settings of the room or chamber are stated.",3,null,2,{"$f":"IF(F69=0,0,INDEX($D69:$D71,F69,1))","v":1},"20°C"]
[null,17,null,1]
[null,null,null,0]
[null,null,"Were test solutions, blanks and/or controls tested for contamination OR were analytical reagent grade chemicals used or the highest possible purity chemicals used for the experiment? (3 marks).",3,null,1,{"$f":"IF(F72=0,0,INDEX($D72:$D73,F72,1))","v":3},"Analytical reagent grade boric acid"]
[null,18,null,0]
[]
[null,null,null,null,null,null,null,null,{"$f":"(COUNTIF(F12:F73,\"=0\"))+COUNTIF(F2:F10,\"=0\")+COUNTIF(D2:D7,\"=0\")","v":0}]
[]
[]
[null,null,"Total score",null,null,null,{"$f":"IF(SUM(G12:G72)=0,\"\",SUM(G12:G72))","v":56}]
[null,null,"Total Max. possible score for the various types of data and contaminants ",null,{"$f":"D12+D15+D18+D21+D23+D26+D29+D32+D35+D38+D41+D44+D47+D50+D53+D57+D66+D69+D72+D60+D63","v":97}]
[null,null,"Quality Score",null,{"$f":"\"= [Total score / Total possible score] * 100 = \"","v":"= [Total score / Total possible score] * 100 = "},null,{"$f":"IF(ISERROR(G78/E79),#N/A,G78/E79)","v":0.57731958762886593},{"$f":"IF(F41=3,\"The highest possible grade for this datum is acceptable.\",\"\")","v":"The highest possible grade for this datum is acceptable."}]
[]
[null,null,"H = high quality                           Quality score ≥ 80%      "]
[null,null,"A = acceptable quality         > 50% Quality score < 80%      "]
[null,null,"U = unacceptable quality              Quality score ≤ 50%  "]
[]
[3,null,"Click the 'Save & Update' button to save and export the answers to the 'All results' worksheet. "]
[null,null,"Select the 'Reset Answers' button if you wish to clear all answers."]

{"sheet":"FW metal plant_WS","index":8,"n_rows":203,"n_cols":25,"rows_kept":203,"cut":false,"hidden":false,"merged":[]}
[]
["Question No.",1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16.100000000000001,16.2,16.3,16.399999999999999,17,18,"Total Score","Quality Score"]
["Short Questions","Duration of the exposure ","Biological endpoint","Biological effect stated or not","Biological effect quantified or not","Appropriate controls","Replication","Test acceptability criteria","Characteristics of the test organism"," Type of test media","Type of exposure","Contaminant concentrations","Parallel reference toxicant toxicity tests","Concentration–response relationship","Determine the toxicity","NOEC/LOEC or LC/EC/BEC","pH","Hardness","Alkalinity ","Organic carbon concentration","Temperature","Grade or purity"]
["Marks Awarded ","10 or 0","10,5 or 0","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","5 or 0 ","4 or 0","4 or 2 or 0","4 or 2","4 or 2","4 or 0","4 or 2","3 or 1 or 0","3 or 1 or 0","3 or 1 or 0","3 or 1 or 0","3 or 0","3 or 0 "]
["Max. Possible Score",10,10,5,5,5,5,5,5,5,4,4,4,4,4,4,3,3,3,3,3,3,{"$f":"SUM(B5:V5)","v":97}]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","1-1"]
["Scores",10,5,5,5,5,5,5,5,5,4,4,0,4,4,4,3,0,0,0,3,3,79,0.81443298969072164,""]
["Answers","30 days","Shoot growth","NOEC","p 0.05 from stats section in Appendix","Non-toxicant controls","5 replicates","Control CV <20%","5 cm apical stem cutting","Dechlorinated tap water","Static renewal 72h rewals","Measured throughout","","Observable and stated","Yes",0.05,"Initial pH was 6.79-7.69. boric acid reduced pH. At test termination, pH was 7.11-7.58","","","","19.39-24.14, overall mean = 21.67","AR Grade"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","1-2"]
["Scores",10,5,5,5,5,5,5,5,5,4,4,0,4,4,4,3,0,0,0,3,3,79,0.81443298969072164,""]
["Answers","30 days","Shoot weight","NOEC","p 0.05 from stats section in Appendix","Non-toxicant controls","5 replicates","Control CV <20%","5 cm apical stem cutting","Dechlorinated tap water","Static renewal 72h rewals","Measured throughout","","Observable and stated","Yes",0.05,"Initial pH was 6.79-7.69. boric acid reduced pH. At test termination, pH was 7.11-7.58","","","","19.39-24.14, overall mean = 21.67","AR Grade"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","1-3"]
["Scores",10,5,5,5,5,5,5,5,5,4,4,0,4,4,4,3,0,0,0,3,3,79,0.81443298969072164,""]
["Answers","30 days","Node number","NOEC","p 0.05 from stats section in Appendix","Non-toxicant controls","5 replicates","Control CV <20%","5 cm apical stem cutting","Dechlorinated tap water","Static renewal 72h rewals","Measured throughout","","Observable and stated","Yes",0.05,"Initial pH was 6.79-7.69. boric acid reduced pH. At test termination, pH was 7.11-7.58","","","","19.39-24.14, overall mean = 21.67","AR Grade"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","1-4"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,4,3,0,0,0,3,3,84,0.865979381443299,""]
["Answers","30 days","Plant condition, defined, but more qualitative than quantitative data","NOEC","p 0.05 from stats section in Appendix","Non-toxicant controls","5 replicates","Control CV <20%","5 cm apical stem cutting","Dechlorinated tap water","Static renewal 72h rewals","Measured throughout","","Observable and stated","Yes",0.05,"Initial pH was 6.79-7.69. boric acid reduced pH. At test termination, pH was 7.11-7.58","","","","19.39-24.14, overall mean = 21.67","AR Grade"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","1-5"]
["Scores",10,5,5,5,5,5,5,5,5,4,4,0,4,4,4,3,0,0,0,3,3,79,0.81443298969072164,""]
["Answers","30 days","Shoot growth","IC",10,"Non-toxicant controls","5 replicates","Control CV <20%","5 cm apical stem cutting","Dechlorinated tap water","Static renewal 72h rewals","Measured throughout","","Observable and stated","Yes","95% CVs 0.93-6.1 mg/L","Initial pH was 6.79-7.69. boric acid reduced pH. At test termination, pH was 7.11-7.58","","","","19.39-24.14, overall mean = 21.67","AR Grade"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","2-1"]
["Scores",10,10,5,5,5,5,2,0,5,4,4,0,4,4,4,3,0,0,0,1,3,74,0.76288659793814428,""]
["Answers","10 d","Growth rate was calculated by the equation [(log total frond number day 10   log number of fronds day 0)/(time day 10 time day 0)] (Thompson and Swigert, 1997).","NOEC","p 0.05","Control contained 0.5 mg/L B for plant growth","6 reps","USEPA 1996","Fronds","pH stabilised growth medium with reference","Static renewal","Measured throughout.","","","","p 0.05","pH ranged from 5.2-5.8","","","","25 ± 1 °C","B measured in controls and samples"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","2-2"]
["Scores",10,10,5,5,5,5,2,0,5,4,4,0,4,4,4,3,0,0,0,1,3,74,0.76288659793814428,""]
["Answers","10 d","The appearance of chlorosis (a progression of green to yellow color on thefronds), necrosis (decaying tissue surrounded by healthy tissue), and death (no living tissue) was observed and classified as abnormal fronds.","NOEC","p 0.05","Control contained 0.5 mg/L B for plant growth","6 reps","USEPA 1996","Fronds","pH stabilised growth medium with reference","Static renewal","Measured throughout.","","","","p 0.05","pH ranged from 5.2-5.8","","","","25 ± 1 °C","B measured in controls and samples"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","2-3"]
["Scores",10,10,5,5,5,5,2,0,5,4,4,0,4,4,4,3,0,0,0,1,3,74,0.76288659793814428,""]
["Answers","10 d","The appearance of chlorosis (a progression of green to yellow color on thefronds), necrosis (decaying tissue surrounded by healthy tissue), and death (no living tissue) was observed and classified as abnormal fronds.","NOEC. Note NOEC reported as 0.9 mg/L, as this was the concentration of B in Control. Unreliable as a real NOEC.","p 0.05","Control contained 0.5 mg/L B for plant growth","6 reps","USEPA 1996","Fronds","pH stabilised growth medium with reference","Static renewal","Measured throughout.","","","","p 0.05","pH ranged from 5.2-5.8","","","","25 ± 1 °C","B measured in controls and samples"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","5-1"]
["Scores",10,10,5,0,5,0,0,0,5,4,0,0,4,0,0,0,0,0,0,1,0,44,0.45360824742268041,"The highest possible grade for this datum is acceptable."]
["Answers","96 h","Incorrectly stated as growth, but it is biomass (dry weight). No growth rate calcualted.","NOEC (although not really stated, but text describes it).","no statistics","B-free controls","Not stated","","","Rodriguez Lopex medium","Static","B was measured in dried cells, but not in test waters","","","No statistics","","","","","","26°C",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","6-1"]
["Scores",10,5,5,0,5,0,0,0,5,4,0,0,4,0,0,0,0,0,0,1,0,39,0.40206185567010311,"The highest possible grade for this datum is acceptable."]
["Answers","72 h","Growth. May be defined but in spanish.","NOEC, not specified, but significant difference to control in text is described","no statistics","non-toxicant cotnrol","Not stated","","","Rodriguez Lopez medium","Assume static","Maybe but in spanish. Doesn't seem to be any measured results however. Methods section very small.","","","No statistics","No statistics","","","","","26°C","boric acid"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","7-1"]
["Scores",0,5,5,0,5,0,0,0,5,4,0,0,0,0,0,0,0,0,0,0,3,27,0.27835051546391754,"The highest possible grade for this datum is acceptable."]
["Answers","3-4 months","Growth","NOEC - not stated, but inferred","no statistics","non-toxicant control","","","","mineral media","Static","","","","","","","","","","","AR grade"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","8-1"]
["Scores",0,10,5,0,5,5,0,5,5,4,2,0,0,0,0,1,0,0,0,1,0,43,0.44329896907216493,""]
["Answers","ranged from 4-7d","Growth defined as biomass and final cell number","NOEC, but inferred","no staitstics","Non-toxicant control","","","60 cells/cubic millimeter","Mineral medium I and II","Static","Measured in 'some'","","","","",6.8,"","","","25°C",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","9-1"]
["Scores",10,10,5,0,5,5,0,5,5,4,0,0,4,0,0,1,0,0,0,1,0,55,0.5670103092783505,"The highest possible grade for this datum is acceptable."]
["Answers","24 d","biomass (dry weight)","NOEC inferred (from statement of statistical significance)","no statistics","Non-B control","four replicates","","Several generations established without B in media, then inoculum is 60 cells/cu mm.","Synthetic water - recipe provided.","Static","","","Partial. Hormesis at 0.5 mg/L then decrease, but only 3 concentrations tested altogether.","No statistics described, despite stating 'significnat' differences","no mention of stats","pH measured but not reported","","","",24,""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","9-2"]
["Scores",10,10,5,0,5,5,0,5,5,4,0,0,4,0,0,1,0,0,0,1,0,55,0.5670103092783505,"The highest possible grade for this datum is acceptable."]
["Answers","24 d","Growth determined by cell counts with haemocytometer","NOEC inferred (from statement of statistical significance)","no statistics","Non-B control","four replicates","","Several generations established without B in media, then inoculum is 60 cells/cu mm.","Synthetic water - recipe provided.","Static","","","Partial. Hormesis at 0.5 mg/L then decrease, but only 3 concentrations tested altogether.","No statistics described, despite stating 'significnat' differences","no mention of stats","pH measured but not reported","","","",24,""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","10-1"]
["Scores",10,10,5,0,5,5,5,5,5,4,4,0,4,4,0,3,3,0,0,3,3,78,0.80412371134020622,""]
["Answers","72 h","Equations and methods given for algal counts using particle counter (Coulter).","NOEC","no p value reported","Non-toxicant control","triplicate and 6 controls","Control growth and pH change criteria, as per OECD.","1x10^4 cells/mL of exponentially growing cells as per OECD guideline","Media - recipe provided","Static, with constant shaking","Beginning and end. Values were within 20% of nominal, therefore nominal used for calcuations","","","","significance level not given.","7.5-8.3","24 mg CaCO3/L","","","23 ± 2°C","Manufacturing grade 99.9% Boric acid."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","10-2"]
["Scores",10,10,5,5,5,5,5,5,5,4,4,0,4,4,0,3,3,0,0,3,3,83,0.85567010309278346,""]
["Answers","72 h","Equations and methods given for algal counts using particle counter (Coulter).","EC",10,"Non-toxicant control","triplicate and 6 controls","Control growth and pH change criteria, as per OECD.","1x10^4 cells/mL of exponentially growing cells as per OECD guideline","Media - recipe provided","Static, with constant shaking","Beginning and end. Values were within 20% of nominal, therefore nominal used for calcuations","","","","no confidence limits","7.5-8.3","24 mg CaCO3/L","","","23 ± 2°C","Manufacturing grade 99.9% Boric acid."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","10-3"]
["Scores",10,10,5,0,5,5,5,5,5,4,4,0,4,4,4,3,3,0,0,3,3,82,0.84536082474226804,""]
["Answers","72 h","Equations and methods given for algal counts using particle counter (Coulter).","NEC","p not given","Non-toxicant control","triplicate and 6 controls","Control growth and pH change criteria, as per OECD.","1x10^4 cells/mL of exponentially growing cells as per OECD guideline","Media - recipe provided","Static, with constant shaking","Beginning and end. Values were within 20% of nominal, therefore nominal used for calcuations","","","","145-167 mg boric acid/L = 25-29 mg/L","7.5-8.3","24 mg CaCO3/L","","","23 ± 2°C","Manufacturing grade 99.9% Boric acid."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","18-1"]
["Scores",10,10,5,5,5,0,0,5,5,4,2,0,4,4,4,3,3,3,0,3,3,78,0.80412371134020622,""]
["Answers","28 d","Biomass - dry weight.","NOEC (LOEC stated, NOEC inferred).","p 0.05","Diluent controls","Controls in triplicate, but rest not replicated.","","10 cm cuttings","Modified Ruakura nutrient solution","Static renewal - 7d renewals","Once at end of week 2.","","","Note: Statistics method different to current day approaches.",0.05,"7.7-10.1","Based on measured Ca and Mg - I calculated hardness to be 23 mg/L","5.2 mg/L CaCO3","","18.5-21.4","B in controls was 0.1 mg/L."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","21-1"]
["Scores",10,10,0,0,5,0,0,5,5,4,0,0,4,0,0,1,0,0,0,0,0,44,0.45360824742268041,"The highest possible grade for this datum is acceptable."]
["Answers","7 d","Growth of was measured as the rate of elongation of the youngest internode using a horizontally mounted microscope.","","","APW controls","Not specified","","Chara shoots, containing two to three of the apical internodes with attatched whorls.","Buffered artificial pond water","static renewal, but frequency of renewal not stated. ","","","","","","6.0 in the media","","","","",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","23-1"]
["Scores",10,10,5,5,5,5,0,5,5,0,0,0,4,4,4,1,0,0,0,1,3,67,0.69072164948453607,"The highest possible grade for this datum is acceptable."]
["Answers","14 d","Growth rate and yield, equations provided for both.","NOEC","p 0.05","Diluent controls","triplicate","","late log phase","MCM media","","","","","",0.05,"media pH stated as 6.8, pH said to be measured in test but not reported.","","","","25 ± 1 °C","Reagent grade"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","25-1"]
["Scores",10,5,5,5,5,5,0,0,5,0,0,0,4,0,4,0,0,0,0,1,0,49,0.50515463917525771,"The highest possible grade for this datum is acceptable."]
["Answers","96 h (from graph, not stated elsewhere)","Culture density determined spectrophotometrically. Dry weight also detremined. However, how 'growth rate' was determined is not provided. Graph for growth rate shows units as mg/ml/h. I think they have used dry weight divided by time.","NOEC from text description","mds tested at 5%","","triplicate","","","Kratz and Myers medium","Assume static","","","","non-standard statistics. Very little description of stats. Only that 'Single hierarchial model' used. And mds at 5% is reported. Significance of results reported, but not which result is significantly different from which.",0.05,"","","","","26°C",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-1"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","filtered Lake Ohakuri water (which had mean of 0.47 mg/L)","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-2"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","filtered Lake Ohakuri water (which had mean of 0.47 mg/L)","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-3"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-4"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-5"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-6"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-7"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-8"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-9"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-10"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-11"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-12"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-13"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-14"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-15"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-16"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-17"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-18"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-19"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-20"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-21"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-22"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-23"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-24"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-25"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-26"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,""]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","NOEC from text and statistics","p < 0.05","non-toxicant controls","four replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","",0.05,"","","","","20 °C","Analytical reagent grade."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-27"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,"The highest possible grade for this datum is acceptable."]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","LOEC from text and statistics","p < 0.05","non-toxicant controls","5 replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","","p 0.05","","","","","20°C","Analytical reagent grade boric acid"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-28"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,"The highest possible grade for this datum is acceptable."]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","LOEC from text and statistics","p < 0.05","non-toxicant controls","5 replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","","p 0.05","","","","","20°C","Analytical reagent grade boric acid"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","26-29"]
["Scores",0,10,5,5,5,5,0,5,5,0,0,0,4,4,4,0,0,0,0,1,3,56,0.57731958762886593,"The highest possible grade for this datum is acceptable."]
["Answers","Not possible to define, as endpoint is wherever and whenever maximum growth rate and maximum yield was obtained.","Growth rate defined by umax equaiton, using daily Chl a measurements. Yield (biomass) is defined as wherevr the maximum Chl a was measured. Chl a measurements used.","LOEC from text and statistics","p < 0.05","non-toxicant controls","5 replicates","","1 x 10^5 cells/mL","1/10th Woods Hole Medium","","Scored 0 because although concs were measured beginning and end, these were not reported anywhere.","","","","p 0.05","","","","","20°C","Analytical reagent grade boric acid"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","27-5"]
["Scores",10,5,5,5,5,5,2,5,0,0,2,0,4,4,4,3,3,1,0,1,3,67,0.69072164948453607,""]
["Answers","96 h","Growth","NOEC","p 0.05","Diluent controls","triplicate","Blaise et al 1986","20000 cells/mL innoculum","However, analytes measured in dilution water are provided and it states media was prepared without EDTA for testing.","Assume static","Stock measured once: 32 mg/L nominal was 34 mg/L and 180 mg/L stock was measured as 186 mg/L.","","","","","diluent pH was 7.4, but report states that pH for all tests ranged from 7.5-8.5","9.3 mg/L CaCO3","<0.5 mg/L","","24 ± 2°C","Dilution water measured to have 0.02 mg/L B"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","27-6"]
["Scores",10,5,5,5,5,5,2,5,0,0,2,0,4,4,4,3,3,1,0,1,3,67,0.69072164948453607,""]
["Answers","96 h","Growth","MATC","p 0.05","Diluent controls","triplicate","Blaise et al 1986","20000 cells/mL innoculum","However, analytes measured in dilution water are provided and it states media was prepared without EDTA for testing.","Assume static","Stock measured once: 32 mg/L nominal was 34 mg/L and 180 mg/L stock was measured as 186 mg/L.","","","","","diluent pH was 7.4, but report states that pH for all tests ranged from 7.5-8.5","9.3 mg/L CaCO3","<0.5 mg/L","","24 ± 2°C","Dilution water measured to have 0.02 mg/L B"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","27-7"]
["Scores",10,5,5,5,5,5,2,5,0,0,2,0,4,4,4,3,3,1,0,1,3,67,0.69072164948453607,""]
["Answers","8 d","Growth","NOEC","p 0.05","Diluent controls","triplicate","Blaise et al 1986","20000 cells/mL innoculum","However, analytes measured in dilution water are provided and it states media was prepared without EDTA for testing.","Assume static","Stock measured once: 32 mg/L nominal was 34 mg/L and 180 mg/L stock was measured as 186 mg/L.","","","","","diluent pH was 7.4, but report states that pH for all tests ranged from 7.5-8.5","9.3 mg/L CaCO3","<0.5 mg/L","","24 ± 2°C","Dilution water measured to have 0.02 mg/L B"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","27-8"]
["Scores",10,5,5,5,5,5,2,5,0,0,2,0,4,4,4,3,3,1,0,1,3,67,0.69072164948453607,""]
["Answers","8 d","Growth","MATC","p 0.05","Diluent controls","triplicate","Blaise et al 1986","20000 cells/mL innoculum","However, analytes measured in dilution water are provided and it states media was prepared without EDTA for testing.","Assume static","Stock measured once: 32 mg/L nominal was 34 mg/L and 180 mg/L stock was measured as 186 mg/L.","","","","","diluent pH was 7.4, but report states that pH for all tests ranged from 7.5-8.5","9.3 mg/L CaCO3","<0.5 mg/L","","24 ± 2°C","Dilution water measured to have 0.02 mg/L B"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","28-1"]
["Scores",10,10,0,5,0,5,2,5,5,0,0,0,0,4,4,3,0,0,0,1,0,54,0.55670103092783507,"The highest possible grade for this datum is acceptable."]
["Answers","21 d","During the experimental period, daily counts were made of germinated seeds, being those that under the stereoscopic magnifying glass presented a radicle of at least 0.1 mm in length.","But NOEC can be derived from the stats done (Kruskal Wallis), using lowest B conc as Control.","p 0.05","Although lowest test conc can be used as control (1 mg/L) as it is in the range of that known for essentiality.","Four replicates","International Seed Testing Association 2008","Average seed size was 1.4 x 0.5 mm. Seeds were then pre-treated: stored in jars filled with river water at 5°C for 3 weeks.","Distilled water ","","","","","","p 0.05","6.19-6.36","","","","13h 35°C, 11h 10°C",""]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","29-1"]
["Scores",10,10,5,5,5,5,5,5,5,4,0,0,4,4,4,0,0,0,0,1,3,75,0.77319587628865982,"The highest possible grade for this datum is acceptable."]
["Answers","7 d","growth in plants was based on frond number. Specifically, all visible fronds were counted manually every day (OECD, 2006). Fresh weight determined by surface-drying between layers of paper towels for 2 min, and fresh weight was determined. After fresh weight determination, fronds were dried at 80  C to measure dry as weight described by Radic et al. (2011).","Stated in text as not significantly different to controls (NOEC).","p 0.05","Diluent controls with 2 mg/L B","triplicate","OECD, and results given for controls meeting acceptability growth criteria","3-frond colonies","SIS media","static","","","","","","","","","","24 ± 2 °C",">99% purity boric acid"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","29-2"]
["Scores",10,10,5,5,5,5,5,5,5,4,0,0,4,4,4,0,0,0,0,1,3,75,0.77319587628865982,"The highest possible grade for this datum is acceptable."]
["Answers","7 d","growth in plants was based on frond number. Specifically, all visible fronds were counted manually every day (OECD, 2006). Fresh weight determined by surface-drying between layers of paper towels for 2 min, and fresh weight was determined. After fresh weight determination, fronds were dried at 80  C to measure dry as weight described by Radic et al. (2011).","Stated in text as not significantly different to controls (NOEC).","p 0.05","Diluewnt controls with 2 mg/L B","triplicate","OECD, and results given for controls meeting acceptability growth criteria","3-frond colonies","SIS media","static","","","","","","","","","","24 ± 2 °C",">99% purity boric acid"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","29-3"]
["Scores",10,10,5,5,5,5,5,5,5,4,0,0,4,4,4,0,0,0,0,1,3,75,0.77319587628865982,"The highest possible grade for this datum is acceptable."]
["Answers","7 d","growth in plants was based on frond number. Specifically, all visible fronds were counted manually every day (OECD, 2006). Fresh weight determined by surface-drying between layers of paper towels for 2 min, and fresh weight was determined. After fresh weight determination, fronds were dried at 80  C to measure dry as weight described by Radic et al. (2011).","Stated in text as not significantly different to controls (NOEC).","p 0.05","Diluewnt controls with 2 mg/L B","triplicate","OECD, and results given for controls meeting acceptability growth criteria","3-frond colonies","SIS media","static","","","","","","","","","","24 ± 2 °C",">99% purity boric acid"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","29-4"]
["Scores",10,10,5,5,5,5,5,5,5,4,0,0,4,4,4,0,0,0,0,1,3,75,0.77319587628865982,"The highest possible grade for this datum is acceptable."]
["Answers","7 d","growth in plants was based on frond number. Specifically, all visible fronds were counted manually every day (OECD, 2006). Fresh weight determined by surface-drying between layers of paper towels for 2 min, and fresh weight was determined. After fresh weight determination, fronds were dried at 80  C to measure dry as weight described by Radic et al. (2011).","Stated in text as not significantly different to controls (NOEC).","p 0.05","Diluewnt controls with 2 mg/L B","triplicate","OECD, and results given for controls meeting acceptability growth criteria","3-frond colonies","SIS media","static","","","","","","","","","","24 ± 2 °C",">99% purity boric acid"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","29-5"]
["Scores",10,10,5,5,5,5,5,5,5,4,0,0,4,4,4,0,0,0,0,1,3,75,0.77319587628865982,"The highest possible grade for this datum is acceptable."]
["Answers","7 d","growth in plants was based on frond number. Specifically, all visible fronds were counted manually every day (OECD, 2006). Fresh weight determined by surface-drying between layers of paper towels for 2 min, and fresh weight was determined. After fresh weight determination, fronds were dried at 80  C to measure dry as weight described by Radic et al. (2011).","Stated in text as not significantly different to controls (NOEC).","p 0.05","Diluewnt controls with 2 mg/L B","triplicate","OECD, and results given for controls meeting acceptability growth criteria","3-frond colonies","SIS media","static","","","","","","","","","","24 ± 2 °C",">99% purity boric acid"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","29-6"]
["Scores",10,10,5,5,5,5,5,5,5,4,0,0,4,4,4,0,0,0,0,1,3,75,0.77319587628865982,"The highest possible grade for this datum is acceptable."]
["Answers","7 d","growth in plants was based on frond number. Specifically, all visible fronds were counted manually every day (OECD, 2006). Fresh weight determined by surface-drying between layers of paper towels for 2 min, and fresh weight was determined. After fresh weight determination, fronds were dried at 80  C to measure dry as weight described by Radic et al. (2011).","Stated in text as not significantly different to controls (NOEC).","p 0.05","Diluewnt controls with 2 mg/L B","triplicate","OECD, and results given for controls meeting acceptability growth criteria","3-frond colonies","SIS media","static","","","","","","","","","","24 ± 2 °C",">99% purity boric acid"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","30-1"]
["Scores",10,10,5,5,5,5,0,0,5,0,0,0,4,4,4,0,0,0,0,1,0,58,0.59793814432989689,"The highest possible grade for this datum is acceptable."]
["Answers","10 d","Dry weight. Samples were dried at 80°C for 72 h.","Text describes concs for which there were no significant differences = NOEC","p 0.05","","triplicate","","","1 in 40 Hoaglands medium. Note Hoaglands has 0.5 mg/L B in it. So 1 in 40 dilution has 0.0125 mg/L B","Assume static","","","","","p 0.05","","","","","25-29°C","boric acid"]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","31-1"]
["Scores",10,5,5,5,5,5,5,0,5,4,2,4,4,4,4,3,3,3,0,1,3,80,0.82474226804123707,""]
["Answers","7 d","Growth, text in report says that final number of fronds counted and used as biomass, however, stats use specific growth rate, therefore unclear.","EC",10,"Diluent (River water) control and SIS control","four replicates","","","Condomine River water (analysis provided)","Static (in brochure in Appendix)","Measured at start","Kcl","","","","in Appendix: 7.0-7.2","81 mg CaCO3/L","89 mg/L","","25 ± 1°C","Boric acid, Controls measured to have 0.2mg/L."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","31-2"]
["Scores",10,5,5,5,5,5,5,0,5,4,2,4,4,4,4,3,3,3,0,1,3,80,0.82474226804123707,""]
["Answers","7 d","Growth, text in report says that final number of fronds counted and used as biomass, however, stats use specific growth rate, therefore unclear.","EC",10,"Diluent (River water) control and SIS control","four replicates","","","Condomine River water (analysis provided)","Static (in brochure in Appendix)","Measured at start","Kcl","","","","in Appendix: 7.0-7.2","81 mg CaCO3/L","89 mg/L","","25 ± 1°C","Boric acid, Controls measured to have 0.2mg/L."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","31-3"]
["Scores",10,5,5,5,5,5,5,0,5,4,2,4,4,4,4,3,3,3,0,1,3,80,0.82474226804123707,""]
["Answers","72 h","","IC",10,"Diluent (River water) control and USEPA media control","four replicates","","","Condomine River water (analysis provided)","Static (in brochure in Appendix)","Measured at start","Kcl","NOTE: hormesis at low B concentrations","","9.9-13 95% Cls","in Appendix: 7.7-8.1","81 mg CaCO3/L","89 mg/L","","25 ± 1°C","Boric acid, Controls measured to have 0.07mg/L."]
["Category","Freshwater/metal/plant","Paper and data unique identifier(eg. 1-1,10-150)","31-4"]
["Scores",10,5,5,5,5,5,5,0,5,4,2,4,4,4,4,3,3,3,0,1,3,80,0.82474226804123707,""]
["Answers","72 h","","NOEC","p 0.05","Diluent (River water) control and USEPA media control","four replicates","","","Condomine River water (analysis provided)","Static (in brochure in Appendix)","Measured at start","Kcl","NOTE: hormesis at low B concentrations","","p 0.05","in Appendix: 7.7-8.1","81 mg CaCO3/L","89 mg/L","","25 ± 1°C","Boric acid, Controls measured to have 0.07mg/L."]